Rh2BG303300

Dolichol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008091
Physical Location & Seq
Reverse (-)
35122161 .. 35128241
6081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG303300.1

Sequence Viewer

Length: 1263 bp
ATGGCTTCGTTGCTGAACGGAGAGAGAGCCGTCGTGGCGCTGTTCGTCGGCCGCGTCCTCTTCTCTCTCCCGCTCTCTCTCCTCCCCCACGGCCTCGCTCTCTCCCTCCTCGCAATCTCCGCTCTCGTCCTCGACGTCCTCGCCGACTCCTCCACCACTCTCCTCTCCCAATTCAACACCAGGCCCGGTGCTTCCTCAGGTATATTATTGGGCGCTGTCACGTTGCCCACTGTCATGATCTCCAAAATGATACAGCTCTCACGACCTTTCTCACTCAATCAAGTTGCATATCAAGAGCTTGAATCTTTGACAATGCAATACTGGGCCATTTCTGCCAGTTCCTTGAGTGTACTTATATTCCTCTGCATCACTATATGGTGTTCACCTGGGAATAAGCCTCCCCCTTCTTTACGCAGTGTTTGGCATGCAATTTTAAGCTTAAGTTGCATAGTATTGCACACAGCAGTGTGTTGCGTAACTCTTGGTACATCATCTCTTACAAGCTCTGAGGCTGTGTTGAAGTTATTGTGGATGCTCTGTCATGGATTGGCAGTTGTAAAATTAATTCAACATGTTTTCAGAACTTTTCCATTTTGTGCTTCGATTGGGGAAGCATGTCTAGTGGCTTCAGGTCTTGTTCTCTATTTTGGTGACATGTTGGCATATACTATTGCAAAGGTGTCTGGCTTCTCAATTAAATCAGATATGGTACAATATGGAAGTAAAACAAATGAGATAAATATTATTATACAGGGTTTGCTGCTTGGCCTTCTTCTTTTTCCAGTGGTCTTTAAACTTGTTCTCCGAATGTGGGAATGCTCTTTCGGTATGGCCCTCTCTGAAGTGAGGACAAACAATGAAACTTGGAGATCAGTTATATTCTTTTCGTCTCTCGGATTTATCATGATTGTGATCATTCCAGCGTGGATGCAGTTTGTTCAGGATTTTCATATGCATCCGTTCTTATGGGTACTGTCATTTATTTTTTCGGAACCACTCAAGAGATTATCTCTGTGTGGCTACTGGGTTTGTGTGATATGTGTGTCTGTTTTGAGGTTCTACAACATTTCAATGAATAGTAAGATTGAGAGAATTCTACTCCGAAAATACTACCATTTGATGGCTGTTTTAATGTTTGTGCCTGCTCTTATCTTCCAGCCAGAGTTTCTTGATCTAGCATTTGGTGCAGCTTTGGCAGTTTTTTTGGCATTGGAAATTGTTCGAGTAAGTTGTCAGAATGGCCAGTTTAATCCATATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko01100 map00510 map01100
Pfam Domains
Protein Families
No domains found.

Protein Analysis

420

Amino Acids

46.55

Weight (kDa)

8.66

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB7I CCANNNNNTGG 1 cut(s) 1120
AccBSI CCGCTC 2 cut(s) 73, 122
AccII CGCG 1 cut(s) 54
AciI CCGC 3 cut(s) 52, 71, 120
AcoI YGGCCR 2 cut(s) 49, 1240
AcsI RAATTY 1 cut(s) 1092
AcuI CTGAAG 2 cut(s) 612, 861
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 4 cut(s) 351, 487, 711, 972
AfiI CCNNNNNNNGG 2 cut(s) 811, 1120
AflII CTTAAG 1 cut(s) 439
AflIII ACRYGT 2 cut(s) 571, 654
AgsI TTSAA 5 cut(s) 175, 302, 520, 569, 1071
AjnI CCWGG 2 cut(s) 179, 385
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 5 cut(s) 256, 298, 438, 504, 1190
AluI AGCT 5 cut(s) 256, 298, 438, 504, 1190
Alw26I GTCTC 1 cut(s) 894
AoxI GGCC 7 cut(s) 49, 91, 182, 324, 766, 831, 1240
ApeKI GCWGC 2 cut(s) 760, 1187
ApoI RAATTY 1 cut(s) 1092
AseI ATTAAT 1 cut(s) 563
Asp700I GAANNNNTTC 1 cut(s) 1218
AspLEI GCGC 2 cut(s) 40, 215
AspS9I GGNCC 3 cut(s) 183, 324, 832
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 375, 662
AxyI CCTNAGG 1 cut(s) 196
BalI TGGCCA 1 cut(s) 1242
BbvI GCAGC 2 cut(s) 747, 1199
BccI CCATC 1 cut(s) 1114
BceAI ACGGC 2 cut(s) 14, 106
BciT130I CCWGG 2 cut(s) 181, 387
BclI TGATCA 1 cut(s) 912
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 894
BfaI CTAG 2 cut(s) 620, 1175
BfoI RGCGCY 2 cut(s) 41, 216
BfrI CTTAAG 1 cut(s) 439
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 3 cut(s) 52, 761, 1188
BlsI GCNGC 3 cut(s) 53, 762, 1189
Bme1390I CCNGG 3 cut(s) 181, 186, 387
BmgT120I GGNCC 3 cut(s) 183, 324, 832
BmiI GGNNCC 1 cut(s) 993
BmrFI CCNGG 3 cut(s) 181, 186, 387
BmrI ACTGGG 2 cut(s) 331, 1033
BmsI GCATC 4 cut(s) 375, 522, 918, 964
BmuI ACTGGG 2 cut(s) 331, 1033
BplI GAGNNNNNCTC 2 cut(s) 994, 1026
BpuEI CTTGAG 2 cut(s) 364, 983
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 911
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 88, 386
BsaXI ACNNNNNCTCC 2 cut(s) 786, 816
Bsc4I CCNNNNNNNGG 2 cut(s) 811, 1120
Bse1I ACTGG 5 cut(s) 326, 336, 782, 1028, 1243
Bse21I CCTNAGG 1 cut(s) 196
Bse8I GATNNNNATC 1 cut(s) 911
BseBI CCWGG 2 cut(s) 181, 387
BseDI CCNNGG 2 cut(s) 88, 386
BseGI GGATG 3 cut(s) 537, 933, 955
BseJI GATNNNNATC 1 cut(s) 911
BseLI CCNNNNNNNGG 2 cut(s) 811, 1120
BseMII CTCAG 2 cut(s) 210, 498
BseNI ACTGG 5 cut(s) 326, 336, 782, 1028, 1243
BseRI GAGGAG 4 cut(s) 71, 98, 139, 152
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 2 cut(s) 747, 1199
BsgI GTGCAG 1 cut(s) 1206
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 7 cut(s) 51, 93, 184, 326, 768, 833, 1242
BsiEI CGRYCG 1 cut(s) 52
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 2 cut(s) 811, 1120
BsmAI GTCTC 1 cut(s) 894
BsmBI CGTCTC 1 cut(s) 894
BsmI GAATGC 1 cut(s) 821
BsnI GGCC 7 cut(s) 51, 93, 184, 326, 768, 833, 1242
Bsp143I GATC 4 cut(s) 237, 869, 912, 1171
BspACI CCGC 3 cut(s) 52, 71, 120
BspANI GGCC 7 cut(s) 51, 93, 184, 326, 768, 833, 1242
BspCNI CTCAG 2 cut(s) 209, 499
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 2 cut(s) 234, 903
BspLI GGNNCC 1 cut(s) 993
BspTI CTTAAG 1 cut(s) 439
BsrBI CCGCTC 2 cut(s) 73, 122
BsrI ACTGG 5 cut(s) 326, 336, 782, 1028, 1243
BssECI CCNNGG 2 cut(s) 88, 386
BssMI GATC 4 cut(s) 237, 869, 912, 1171
BssNI GRCGYC 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 181, 387
Bst4CI ACNGT 2 cut(s) 232, 975
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 135
BstAFI CTTAAG 1 cut(s) 439
BstAPI GCANNNNNTGC 1 cut(s) 1184
BstC8I GCNNGC 2 cut(s) 426, 1143
BstDEI CTNAG 2 cut(s) 196, 507
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 3 cut(s) 537, 933, 955
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 2 cut(s) 41, 216
BstHHI GCGC 2 cut(s) 40, 215
BstKTI GATC 4 cut(s) 240, 872, 915, 1174
BstMAI GTCTC 1 cut(s) 894
BstMBI GATC 4 cut(s) 237, 869, 912, 1171
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 6 cut(s) 35, 119, 332, 444, 1184, 1193
BstNI CCWGG 2 cut(s) 181, 387
BstNSI RCATGY 4 cut(s) 428, 575, 618, 658
BstSCI CCNGG 3 cut(s) 179, 184, 385
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 747, 1199
BstZI CGGCCG 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 196
BsuRI GGCC 7 cut(s) 51, 93, 184, 326, 768, 833, 1242
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 3 cut(s) 537, 933, 955
BtsI GCAGTG 2 cut(s) 421, 471
BtsIMutI CAGTG 4 cut(s) 228, 421, 471, 789
Cac8I GCNNGC 2 cut(s) 426, 1143
CciI TCATGA 2 cut(s) 234, 903
CfoI GCGC 2 cut(s) 40, 215
Cfr13I GGNCC 3 cut(s) 183, 324, 832
CseI GACGC 1 cut(s) 43
Csp6I GTAC 4 cut(s) 350, 486, 710, 971
CviAII CATG 7 cut(s) 235, 425, 542, 572, 615, 655, 904
CviQI GTAC 4 cut(s) 350, 486, 710, 971
DdeI CTNAG 2 cut(s) 196, 507
DpnI GATC 4 cut(s) 239, 871, 914, 1173
DpnII GATC 4 cut(s) 237, 869, 912, 1171
DraI TTTAAA 1 cut(s) 793
EaeI YGGCCR 2 cut(s) 49, 1240
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EclXI CGGCCG 1 cut(s) 49
Eco52I CGGCCG 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 612, 861
Eco81I CCTNAGG 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 1092
EcoRII CCWGG 2 cut(s) 179, 385
EcoT22I ATGCAT 1 cut(s) 957
Esp3I CGTCTC 1 cut(s) 894
FaeI CATG 7 cut(s) 238, 428, 545, 575, 618, 658, 907
FatI CATG 7 cut(s) 234, 424, 541, 571, 614, 654, 903
FauI CCCGC 1 cut(s) 78
FauNDI CATATG 1 cut(s) 951
FbaI TGATCA 1 cut(s) 912
Fnu4HI GCNGC 3 cut(s) 52, 761, 1188
FokI GGATG 3 cut(s) 544, 940, 942
Fsp4HI GCNGC 3 cut(s) 52, 761, 1188
FspBI CTAG 2 cut(s) 620, 1175
GlaI GCGC 2 cut(s) 39, 214
GluI GCNGC 3 cut(s) 52, 761, 1188
HaeII RGCGCY 2 cut(s) 41, 216
HaeIII GGCC 7 cut(s) 51, 93, 184, 326, 768, 833, 1242
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 43
HhaI GCGC 2 cut(s) 40, 215
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 7 cut(s) 238, 428, 545, 575, 618, 658, 907
Hin6I GCGC 2 cut(s) 38, 213
HinP1I GCGC 2 cut(s) 38, 213
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 2 cut(s) 146, 302
HpaII CCGG 1 cut(s) 186
HphI GGTGA 2 cut(s) 375, 662
Hpy166II GTNNAC 2 cut(s) 350, 383
Hpy188I TCNGA 9 cut(s) 508, 581, 703, 806, 841, 896, 991, 1103, 1236
Hpy188III TCNNGA 7 cut(s) 235, 261, 293, 904, 941, 1000, 1169
Hpy8I GTNNAC 2 cut(s) 350, 383
Hpy99I CGWCG 3 cut(s) 35, 50, 137
HpyAV CCTTC 2 cut(s) 414, 779
HpyCH4III ACNGT 2 cut(s) 232, 975
HpyCH4IV ACGT 2 cut(s) 135, 221
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 119, 332, 444, 1184, 1193
HpyF3I CTNAG 2 cut(s) 196, 507
HpySE526I ACGT 2 cut(s) 135, 221
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 7 cut(s) 238, 428, 545, 575, 618, 658, 907
HspAI GCGC 2 cut(s) 38, 213
Ksp22I TGATCA 1 cut(s) 912
Kzo9I GATC 4 cut(s) 237, 869, 912, 1171
Lsp1109I GCAGC 2 cut(s) 747, 1199
LweI GCATC 4 cut(s) 375, 522, 918, 964
MaeI CTAG 2 cut(s) 620, 1175
MaeII ACGT 2 cut(s) 135, 221
MaeIII GTNAC 3 cut(s) 217, 475, 650
MalI GATC 4 cut(s) 239, 871, 914, 1173
MbiI CCGCTC 2 cut(s) 73, 122
MboI GATC 4 cut(s) 237, 869, 912, 1171
MboII GAAGA 3 cut(s) 52, 764, 1144
MlsI TGGCCA 1 cut(s) 1242
MluCI AATT 7 cut(s) 170, 429, 560, 564, 693, 1092, 1215
MluNI TGGCCA 1 cut(s) 1242
MlyI GAGTC 1 cut(s) 140
Mox20I TGGCCA 1 cut(s) 1242
Mph1103I ATGCAT 1 cut(s) 957
MroXI GAANNNNTTC 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 1242
MseI TTAA 7 cut(s) 434, 440, 563, 696, 792, 1130, 1248
MslI CAYNNNNRTG 4 cut(s) 233, 464, 908, 1070
Msp20I TGGCCA 1 cut(s) 1242
MspCI CTTAAG 1 cut(s) 439
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 181, 186, 387
Mva1269I GAATGC 1 cut(s) 821
MvaI CCWGG 2 cut(s) 181, 387
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 6 cut(s) 35, 119, 332, 444, 1184, 1193
NciI CCSGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 951
NdeII GATC 4 cut(s) 237, 869, 912, 1171
NlaIII CATG 7 cut(s) 238, 428, 545, 575, 618, 658, 907
NlaIV GGNNCC 1 cut(s) 993
NmuCI GTSAC 2 cut(s) 217, 650
NsiI ATGCAT 1 cut(s) 957
NspI RCATGY 4 cut(s) 428, 575, 618, 658
OliI CACNNNNGTG 1 cut(s) 464
PaeI GCATGC 1 cut(s) 428
PagI TCATGA 2 cut(s) 234, 903
PciI ACATGT 2 cut(s) 571, 654
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 132, 141
PctI GAATGC 1 cut(s) 821
PdmI GAANNNNTTC 1 cut(s) 1218
PfeI GAWTC 1 cut(s) 302
PflMI CCANNNNNTGG 1 cut(s) 1120
PkrI GCNGC 3 cut(s) 53, 762, 1189
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PscI ACATGT 2 cut(s) 571, 654
PshBI ATTAAT 1 cut(s) 563
Psp6I CCWGG 2 cut(s) 179, 385
PspGI CCWGG 2 cut(s) 179, 385
PspN4I GGNNCC 1 cut(s) 993
PspPI GGNCC 3 cut(s) 183, 324, 832
RsaI GTAC 4 cut(s) 351, 487, 711, 972
RsaNI GTAC 4 cut(s) 350, 486, 710, 971
RseI CAYNNNNRTG 4 cut(s) 233, 464, 908, 1070
SaqAI TTAA 7 cut(s) 434, 440, 563, 696, 792, 1130, 1248
SatI GCNGC 3 cut(s) 52, 761, 1188
Sau3AI GATC 4 cut(s) 237, 869, 912, 1171
Sau96I GGNCC 3 cut(s) 183, 324, 832
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 3 cut(s) 181, 186, 387
SfaNI GCATC 4 cut(s) 375, 522, 918, 964
SmiMI CAYNNNNRTG 4 cut(s) 233, 464, 908, 1070
SmlI CTYRAG 3 cut(s) 343, 439, 998
SmoI CTYRAG 3 cut(s) 343, 439, 998
SphI GCATGC 1 cut(s) 428
Sse9I AATT 7 cut(s) 170, 429, 560, 564, 693, 1092, 1215
SsiI CCGC 3 cut(s) 52, 71, 120
SspI AATATT 1 cut(s) 742
SspMI CTAG 2 cut(s) 620, 1175
StyD4I CCNGG 3 cut(s) 179, 184, 385
TaaI ACNGT 2 cut(s) 232, 975
TaiI ACGT 2 cut(s) 138, 224
TaqI TCGA 3 cut(s) 132, 602, 1222
TasI AATT 7 cut(s) 170, 429, 560, 564, 693, 1092, 1215
TatI WGTACW 1 cut(s) 349
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 7 cut(s) 434, 440, 563, 696, 792, 1130, 1248
Tru9I TTAA 7 cut(s) 434, 440, 563, 696, 792, 1130, 1248
TscAI CASTG 4 cut(s) 235, 421, 471, 789
TseFI GTSAC 2 cut(s) 217, 650
TseI GCWGC 2 cut(s) 760, 1187
Tsp45I GTSAC 2 cut(s) 217, 650
TspDTI ATGAA 3 cut(s) 873, 938, 1088
TspGWI ACGGA 2 cut(s) 33, 948
TspRI CASTG 4 cut(s) 235, 421, 471, 789
Van91I CCANNNNNTGG 1 cut(s) 1120
Vha464I CTTAAG 1 cut(s) 439
VspI ATTAAT 1 cut(s) 563
XapI RAATTY 1 cut(s) 1092
XceI RCATGY 4 cut(s) 428, 575, 618, 658
XmnI GAANNNNTTC 1 cut(s) 1218
XspI CTAG 2 cut(s) 620, 1175
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.