Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0012569

Physical Location & Seq

Genomic Coordinates Forward (+)

35668197 .. 35674797

Gene Structure 6601 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018768

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGGGTTGTTCAAGAGCGAGGCTCGTGTGGTTTACGAGCCGGCGAGCCGAGTTGATGTCAGTCCGGCCAGCTCTGAGGTCTACTTGCGAGCCAATGTCAAAGCTCCTCGTATGGCTGGATTTCTGGCCAAATTGTTCGCCTGGTTTTTGGAATCGCGGATCTTTGGGACCGTGTTGTTGTATATATTGAAAAGAGATAACTTGATCCATAAGTTGGTTACAAATGCTGAGCTGGAGGAATCACCAGTCTATGTTCCCTTACATCCTTTTGATGAGATTGAAGAACAAGATGTCAAACATGTAGGGTCTGACTTGTCACCACCTGAATTAGTTCAACAGGCAATTGAATGTTTACCTGAATCCTTTGATAAGGAATTAAATGGTTCAAACTCTTTTTTCCGTTATTGGACAATATCAGATTATTCAAGAGCCTACAAGTCTGGAGAAATAACACCTAAATTGGTTGCAGAGCATCTTATAGCTGCCATCCACGAATCTTCTAATCCACCATTTAAGATGTCATTCTTTATTGATTACAACACGGAAGACATTCTTAAGCAAGCAACTGAATCAACTGTGCGGTATGAAAAAGGTGTGCCTATTTCAGCCCTAGATGGGATCCCAATTGCTATCAAGGATGAAATAGACTGCAGACCATATCCAACTACTGGTGGTACGAAGTGGCTTCACAAATTCAGACCTTGTACAGATGACGCGTGCTGTGTCAAGTGCCTCAGACAATGCGGTGCCATACTTATTGGGAAAACCAATATGCATGAACTTGGAGCTGGTACAAGTGGGATAAATCCGCATTATGGGGCAACTAGAAATCCGCACGACCCCAGCAAAATCTCTGGGGGTTCTTCTAGTGGATCTGCTGCGGTGGTTGCCTCTGGATTATGCCCTGTTGCTCTTGGTGTCGATGGAGGAGGATCTGTGCGGATGCCTGCAGCTCTTTGTGGTGTTGTAGGTTTAAAACCAACCTTTGGCCGAGTACCTCATTCTGGTGTTCTTCCTCTGAATTGGACGGTCGGGATGGTTGGAATACTAGCAGGCACTGTTGAGGATGCTTTAATAGTTTATGCGGCAATCATTGGCCAAATTTCATCAAGTCAACCCACATTATCCCCCAAAGTATATTTTCCTCTATTGAACTCAGCAAAACCTATATCGGATATCAAATTGGCAAAATATGGTGAGTGGTTTAATGATTGCAGTGATGAGATCAGAGAATGCTGTACCAATGCCCTAGACAGGTTTCACAAGCAATATGGTTGGAAGACTGTAGAGGTGACCATACCAGAGATAGAGGTGATGCGGTTGGCACATTACTTGACAATTGGATCAGAGTGTAGCACTTCGCTCAGTTCTCATCTAGAAAAGTTGGATTTCAAAGAATTAGGGTGGGATGTAAGGGTAGGACTTGCTGTGTATGGTGCTTTCAGCAGTAAGGAGTATATACAGGCTCAGAGAATTAGGAACCGGCAGATGCAATTTCACAAGAATTTGTTTTCTCAGGCAGATGTCATTGTTTCCCCAACAGTTGGATACGATAAATCAGGTCTGCCAATTGGTCTTCAGTTGATTGGAAAACCATGGTCTGAACCAACATTAATTCACATAGCCTTTGCAGTGCAGAACCTTTATGTCGCTGATTGCAGAAAGCCCCAGGTCTATTATAATTTGCTGAATACAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0005886 GO:0006066 GO:0006576 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009308 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0016811 GO:0031090 GO:0034308 GO:0034641 GO:0042439 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044106 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0047412 GO:0050896 GO:0051704 GO:0051707 GO:0070291 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidase FPP

Protein Analysis

567

Amino Acids

62.33

Weight (kDa)

6.33

Isoelectric Point (pI)

44.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	181 - 516	1.2e-75	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012569)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25540 FvH4_1g25540 FvH4_1g25540 FvH4_1g25540
malus_domestica	MD15G1139000.v1.1
prunus_persica	Prupe.4G282000_v2.0.a1 Prupe.4G282000_v2.0.a1
pyrus_communis	pycom15g12560
rosa_chinensis	RchiOBHm_Chr2g0123671
rosa_laevigata	RLG00000018768
rosa_multiflora	Rmu_sc0004915.1_g000003
rosa_roxburghii	Rroxscaffold_2G00120450
rosa_rugosa	Rorug02G0247300
rosa_samantha	Rh2AG306300 Rh2BG314500 Rh2CG292700 Rh2DG330000 Rh7CG421300
rosa_wichuraiana	Rw2G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1680
AccB1I	GGYRCC	1 cut(s)	746
AccB7I	CCANNNNNTGG	4 cut(s)	214, 668, 986, 1544
AccI	GTMKAC	1 cut(s)	81
AccII	CGCG	2 cut(s)	157, 716
AciI	CCGC	9 cut(s)	157, 580, 744, 809, 833, 881, 940, 1085, 1318
AclWI	GGATC	7 cut(s)	167, 199, 613, 626, 880, 940, 1351
AcoI	YGGCCR	4 cut(s)	66, 126, 988, 1096
AcsI	RAATTY	3 cut(s)	692, 1101, 1504
AcuI	CTGAAG	1 cut(s)	1562
AfaI	GTAC	5 cut(s)	676, 706, 793, 996, 1240
AfiI	CCNNNNNNNGG	8 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544
AflII	CTTAAG	1 cut(s)	554
AflIII	ACRYGT	2 cut(s)	298, 714
AgsI	TTSAA	9 cut(s)	13, 190, 281, 335, 347, 387, 426, 1153, 1393
AjnI	CCWGG	2 cut(s)	140, 1668
AluBI	AGCT	6 cut(s)	72, 104, 232, 482, 788, 953
AluI	AGCT	6 cut(s)	72, 104, 232, 482, 788, 953
AlwI	GGATC	7 cut(s)	167, 199, 613, 626, 880, 940, 1351
AlwNI	CAGNNNCTG	1 cut(s)	1058
AoxI	GGCC	4 cut(s)	66, 126, 988, 1096
ApeKI	GCWGC	3 cut(s)	482, 878, 950
ApoI	RAATTY	3 cut(s)	692, 1101, 1504
AseI	ATTAAT	1 cut(s)	1613
Asp700I	GAANNNNTTC	2 cut(s)	330, 549
AspS9I	GGNCC	1 cut(s)	168
AsuHPI	GGTGA	5 cut(s)	234, 309, 1208, 1303, 1324
AvaII	GGWCC	1 cut(s)	168
BalI	TGGCCA	2 cut(s)	128, 1098
BamHI	GGATCC	1 cut(s)	618
BanI	GGYRCC	1 cut(s)	746
BauI	CACGAG	1 cut(s)	24
BbsI	GAAGAC	3 cut(s)	552, 1286, 1568
BbvI	GCAGC	3 cut(s)	469, 865, 962
BccI	CCATC	4 cut(s)	494, 608, 917, 1030
BciT130I	CCWGG	2 cut(s)	142, 1670
BciVI	GTATCC	1 cut(s)	1541
BfaI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
BfmI	CTRYAG	3 cut(s)	649, 948, 1284
BfrI	CTTAAG	1 cut(s)	554
BfuI	GTATCC	1 cut(s)	1541
BisI	GCNGC	4 cut(s)	483, 879, 951, 1086
BlpI	GCTNAGC	1 cut(s)	228
BlsI	GCNGC	4 cut(s)	484, 880, 952, 1087
Bme1390I	CCNGG	2 cut(s)	142, 1670
Bme18I	GGWCC	1 cut(s)	168
BmgT120I	GGNCC	1 cut(s)	168
BmiI	GGNNCC	4 cut(s)	169, 620, 748, 1481
BmrFI	CCNGG	2 cut(s)	142, 1670
BmsI	GCATC	5 cut(s)	481, 933, 1057, 1305, 1479
BpiI	GAAGAC	3 cut(s)	552, 1286, 1568
BplI	GAGNNNNNCTC	2 cut(s)	7, 39
BpmI	CTGGAG	2 cut(s)	254, 462
Bpu1102I	GCTNAGC	1 cut(s)	228
BsaJI	CCNNGG	2 cut(s)	1595, 1668
BsaXI	ACNNNNNCTCC	2 cut(s)	921, 951
Bsc4I	CCNNNNNNNGG	8 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544
Bse118I	RCCGGY	2 cut(s)	40, 1482
Bse1I	ACTGG	2 cut(s)	245, 673
BseBI	CCWGG	2 cut(s)	142, 1670
BseDI	CCNNGG	2 cut(s)	1595, 1668
BseGI	GGATG	7 cut(s)	262, 486, 643, 948, 1041, 1072, 1414
BseLI	CCNNNNNNNGG	8 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544
BseMII	CTCAG	7 cut(s)	66, 219, 748, 1170, 1378, 1481, 1529
BseNI	ACTGG	2 cut(s)	245, 673
BseRI	GAGGAG	2 cut(s)	96, 942
BseXI	GCAGC	3 cut(s)	469, 865, 962
BseYI	CCCAGC	1 cut(s)	842
BsgI	GTGCAG	1 cut(s)	1655
Bsh1236I	CGCG	2 cut(s)	157, 716
Bsh1285I	CGRYCG	1 cut(s)	1032
BshFI	GGCC	4 cut(s)	68, 128, 990, 1098
BshNI	GGYRCC	1 cut(s)	746
BsiEI	CGRYCG	1 cut(s)	1032
BsiSI	CCGG	3 cut(s)	41, 65, 1483
BslFI	GGGAC	1 cut(s)	181
BslI	CCNNNNNNNGG	8 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544
BsmFI	GGGAC	1 cut(s)	181
BsmI	GAATGC	1 cut(s)	1238
BsnI	GGCC	4 cut(s)	68, 128, 990, 1098
Bsp1407I	TGTACA	1 cut(s)	704
Bsp143I	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
Bsp1720I	GCTNAGC	1 cut(s)	228
Bsp19I	CCATGG	1 cut(s)	1595
BspACI	CCGC	9 cut(s)	157, 580, 744, 809, 833, 881, 940, 1085, 1318
BspANI	GGCC	4 cut(s)	68, 128, 990, 1098
BspCNI	CTCAG	7 cut(s)	67, 220, 747, 1169, 1377, 1480, 1528
BspFNI	CGCG	2 cut(s)	157, 716
BspLI	GGNNCC	4 cut(s)	169, 620, 748, 1481
BspMAI	CTGCAG	2 cut(s)	653, 952
BspPI	GGATC	7 cut(s)	167, 199, 613, 626, 880, 940, 1351
BspT107I	GGYRCC	1 cut(s)	746
BspTI	CTTAAG	1 cut(s)	554
BsrFI	RCCGGY	2 cut(s)	40, 1482
BsrGI	TGTACA	1 cut(s)	704
BsrI	ACTGG	2 cut(s)	245, 673
BssAI	RCCGGY	2 cut(s)	40, 1482
BssECI	CCNNGG	2 cut(s)	1595, 1668
BssMI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
BssSI	CACGAG	1 cut(s)	24
BssT1I	CCWWGG	1 cut(s)	1595
Bst2BI	CACGAG	1 cut(s)	24
Bst2UI	CCWGG	2 cut(s)	142, 1670
Bst4CI	ACNGT	6 cut(s)	172, 577, 1030, 1060, 1285, 1543
BstAFI	CTTAAG	1 cut(s)	554
BstAUI	TGTACA	1 cut(s)	704
BstC8I	GCNNGC	8 cut(s)	42, 46, 70, 90, 561, 718, 948, 1054
BstDEI	CTNAG	7 cut(s)	75, 228, 734, 1156, 1364, 1467, 1515
BstDSI	CCRYGG	1 cut(s)	1595
BstEII	GGTNACC	1 cut(s)	1291
BstF5I	GGATG	7 cut(s)	262, 486, 643, 948, 1041, 1072, 1414
BstFNI	CGCG	2 cut(s)	157, 716
BstKTI	GATC	7 cut(s)	162, 207, 621, 875, 935, 1227, 1346
BstMBI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
BstMCI	CGRYCG	1 cut(s)	1032
BstMWI	GCNNNNNNNGC	1 cut(s)	887
BstNI	CCWGG	2 cut(s)	142, 1670
BstNSI	RCATGY	1 cut(s)	302
BstPI	GGTNACC	1 cut(s)	1291
BstSCI	CCNGG	2 cut(s)	140, 1668
BstSFI	CTRYAG	3 cut(s)	649, 948, 1284
BstUI	CGCG	2 cut(s)	157, 716
BstV1I	GCAGC	3 cut(s)	469, 865, 962
BstV2I	GAAGAC	3 cut(s)	552, 1286, 1568
BstX2I	RGATCY	4 cut(s)	159, 618, 872, 932
BstYI	RGATCY	4 cut(s)	159, 618, 872, 932
BsuI	GTATCC	1 cut(s)	1541
BsuRI	GGCC	4 cut(s)	68, 128, 990, 1098
BtgI	CCRYGG	1 cut(s)	1595
BtsCI	GGATG	7 cut(s)	262, 486, 643, 948, 1041, 1072, 1414
BtsI	GCAGTG	2 cut(s)	1222, 1638
BtsIMutI	CAGTG	3 cut(s)	1056, 1222, 1638
Cac8I	GCNNGC	8 cut(s)	42, 46, 70, 90, 561, 718, 948, 1054
CaiI	CAGNNNCTG	1 cut(s)	1058
Cfr10I	RCCGGY	2 cut(s)	40, 1482
Cfr13I	GGNCC	1 cut(s)	168
CseI	GACGC	1 cut(s)	722
Csp6I	GTAC	5 cut(s)	675, 705, 792, 995, 1239
CviAII	CATG	3 cut(s)	299, 776, 1596
CviQI	GTAC	5 cut(s)	675, 705, 792, 995, 1239
DdeI	CTNAG	7 cut(s)	75, 228, 734, 1156, 1364, 1467, 1515
DpnI	GATC	7 cut(s)	161, 206, 620, 874, 934, 1226, 1345
DpnII	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
DraI	TTTAAA	1 cut(s)	975
EaeI	YGGCCR	4 cut(s)	66, 126, 988, 1096
Eco130I	CCWWGG	1 cut(s)	1595
Eco32I	GATATC	1 cut(s)	1177
Eco47I	GGWCC	1 cut(s)	168
Eco57I	CTGAAG	1 cut(s)	1562
Eco91I	GGTNACC	1 cut(s)	1291
EcoO65I	GGTNACC	1 cut(s)	1291
EcoRII	CCWGG	2 cut(s)	140, 1668
EcoRV	GATATC	1 cut(s)	1177
EcoT14I	CCWWGG	1 cut(s)	1595
EcoT22I	ATGCAT	1 cut(s)	777
ErhI	CCWWGG	1 cut(s)	1595
FaeI	CATG	3 cut(s)	302, 779, 1599
FalI	AAGNNNNNCTT	2 cut(s)	537, 569
FaqI	GGGAC	1 cut(s)	181
FatI	CATG	3 cut(s)	298, 775, 1595
FblI	GTMKAC	1 cut(s)	81
Fnu4HI	GCNGC	4 cut(s)	483, 879, 951, 1086
FokI	GGATG	7 cut(s)	249, 473, 650, 955, 1048, 1079, 1421
Fsp4HI	GCNGC	4 cut(s)	483, 879, 951, 1086
FspBI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
GluI	GCNGC	4 cut(s)	483, 879, 951, 1086
GsaI	CCCAGC	1 cut(s)	846
GsuI	CTGGAG	2 cut(s)	254, 462
HaeIII	GGCC	4 cut(s)	68, 128, 990, 1098
HapII	CCGG	3 cut(s)	41, 65, 1483
HgaI	GACGC	1 cut(s)	722
Hin1II	CATG	3 cut(s)	302, 779, 1599
HincII	GTYRAC	1 cut(s)	1115
HindII	GTYRAC	1 cut(s)	1115
HinfI	GANTC	5 cut(s)	152, 239, 359, 494, 569
HpaII	CCGG	3 cut(s)	41, 65, 1483
HphI	GGTGA	5 cut(s)	234, 309, 1208, 1303, 1324
Hpy166II	GTNNAC	4 cut(s)	34, 82, 353, 1115
Hpy188III	TCNNGA	6 cut(s)	13, 426, 441, 894, 1033, 1376
Hpy8I	GTNNAC	4 cut(s)	34, 82, 353, 1115
HpyCH4III	ACNGT	6 cut(s)	172, 577, 1030, 1060, 1285, 1543
HpyCH4V	TGCA	9 cut(s)	467, 651, 775, 950, 1215, 1492, 1631, 1636, 1659
HpyF10VI	GCNNNNNNNGC	1 cut(s)	887
HpyF3I	CTNAG	7 cut(s)	75, 228, 734, 1156, 1364, 1467, 1515
Hsp92II	CATG	3 cut(s)	302, 779, 1599
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
Kzo9I	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
LmnI	GCTCC	2 cut(s)	109, 785
Lsp1109I	GCAGC	3 cut(s)	469, 865, 962
LweI	GCATC	5 cut(s)	481, 933, 1057, 1305, 1479
MaeI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
MaeIII	GTNAC	3 cut(s)	217, 315, 1291
MalI	GATC	7 cut(s)	161, 206, 620, 874, 934, 1226, 1345
MboI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
MboII	GAAGA	7 cut(s)	293, 489, 557, 855, 1004, 1291, 1568
MfeI	CAATTG	4 cut(s)	342, 624, 1338, 1569
MflI	RGATCY	4 cut(s)	159, 618, 872, 932
MlsI	TGGCCA	2 cut(s)	128, 1098
MluI	ACGCGT	1 cut(s)	714
MluNI	TGGCCA	2 cut(s)	128, 1098
MmeI	TCCRAC	5 cut(s)	686, 1021, 1256, 1365, 1525
Mox20I	TGGCCA	2 cut(s)	128, 1098
Mph1103I	ATGCAT	1 cut(s)	777
MroNI	GCCGGC	1 cut(s)	40
MroXI	GAANNNNTTC	2 cut(s)	330, 549
MscI	TGGCCA	2 cut(s)	128, 1098
MseI	TTAA	8 cut(s)	377, 513, 555, 974, 1073, 1206, 1613, 1699
MslI	CAYNNNNRTG	1 cut(s)	1005
Msp20I	TGGCCA	2 cut(s)	128, 1098
MspCI	CTTAAG	1 cut(s)	554
MspI	CCGG	3 cut(s)	41, 65, 1483
MspR9I	CCNGG	2 cut(s)	142, 1670
MunI	CAATTG	4 cut(s)	342, 624, 1338, 1569
Mva1269I	GAATGC	1 cut(s)	1238
MvaI	CCWGG	2 cut(s)	142, 1670
MvnI	CGCG	2 cut(s)	157, 716
MwoI	GCNNNNNNNGC	1 cut(s)	887
NaeI	GCCGGC	1 cut(s)	42
NcoI	CCATGG	1 cut(s)	1595
NdeII	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
NgoMIV	GCCGGC	1 cut(s)	40
NlaIII	CATG	3 cut(s)	302, 779, 1599
NlaIV	GGNNCC	4 cut(s)	169, 620, 748, 1481
NmeAIII	GCCGAG	2 cut(s)	74, 1016
NmuCI	GTSAC	2 cut(s)	315, 1291
NsiI	ATGCAT	1 cut(s)	777
NspI	RCATGY	1 cut(s)	302
PciI	ACATGT	1 cut(s)	298
PctI	GAATGC	1 cut(s)	1238
PdiI	GCCGGC	1 cut(s)	42
PdmI	GAANNNNTTC	2 cut(s)	330, 549
PfeI	GAWTC	5 cut(s)	152, 239, 359, 494, 569
PflMI	CCANNNNNTGG	4 cut(s)	214, 668, 986, 1544
PkrI	GCNGC	4 cut(s)	484, 880, 952, 1087
PscI	ACATGT	1 cut(s)	298
PshBI	ATTAAT	1 cut(s)	1613
PsiI	TTATAA	1 cut(s)	1680
Psp6I	CCWGG	2 cut(s)	140, 1668
PspEI	GGTNACC	1 cut(s)	1291
PspFI	CCCAGC	1 cut(s)	842
PspGI	CCWGG	2 cut(s)	140, 1668
PspN4I	GGNNCC	4 cut(s)	169, 620, 748, 1481
PspPI	GGNCC	1 cut(s)	168
PstI	CTGCAG	2 cut(s)	653, 952
PstNI	CAGNNNCTG	1 cut(s)	1058
PsuI	RGATCY	4 cut(s)	159, 618, 872, 932
RsaI	GTAC	5 cut(s)	676, 706, 793, 996, 1240
RsaNI	GTAC	5 cut(s)	675, 705, 792, 995, 1239
RseI	CAYNNNNRTG	1 cut(s)	1005
SaqAI	TTAA	8 cut(s)	377, 513, 555, 974, 1073, 1206, 1613, 1699
SatI	GCNGC	4 cut(s)	483, 879, 951, 1086
Sau3AI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
Sau96I	GGNCC	1 cut(s)	168
ScrFI	CCNGG	2 cut(s)	142, 1670
SfaNI	GCATC	5 cut(s)	481, 933, 1057, 1305, 1479
SfcI	CTRYAG	3 cut(s)	649, 948, 1284
SinI	GGWCC	1 cut(s)	168
SmiMI	CAYNNNNRTG	1 cut(s)	1005
SmlI	CTYRAG	1 cut(s)	554
SmoI	CTYRAG	1 cut(s)	554
SsiI	CCGC	9 cut(s)	157, 580, 744, 809, 833, 881, 940, 1085, 1318
SspMI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
StyD4I	CCNGG	2 cut(s)	140, 1668
StyI	CCWWGG	1 cut(s)	1595
TaaI	ACNGT	6 cut(s)	172, 577, 1030, 1060, 1285, 1543
TaqI	TCGA	1 cut(s)	921
TatI	WGTACW	1 cut(s)	704
TauI	GCSGC	1 cut(s)	1088
TfiI	GAWTC	5 cut(s)	152, 239, 359, 494, 569
Tru1I	TTAA	8 cut(s)	377, 513, 555, 974, 1073, 1206, 1613, 1699
Tru9I	TTAA	8 cut(s)	377, 513, 555, 974, 1073, 1206, 1613, 1699
TscAI	CASTG	3 cut(s)	1063, 1222, 1638
TseFI	GTSAC	2 cut(s)	315, 1291
TseI	GCWGC	3 cut(s)	482, 878, 950
Tsp45I	GTSAC	2 cut(s)	315, 1291
TspDTI	ATGAA	4 cut(s)	600, 654, 792, 1095
TspGWI	ACGGA	2 cut(s)	389, 557
TspRI	CASTG	3 cut(s)	1063, 1222, 1638
Van91I	CCANNNNNTGG	4 cut(s)	214, 668, 986, 1544
Vha464I	CTTAAG	1 cut(s)	554
VpaK11BI	GGWCC	1 cut(s)	168
VspI	ATTAAT	1 cut(s)	1613
XapI	RAATTY	3 cut(s)	692, 1101, 1504
XbaI	TCTAGA	1 cut(s)	1375
XceI	RCATGY	1 cut(s)	302
XmiI	GTMKAC	1 cut(s)	81
XmnI	GAANNNNTTC	2 cut(s)	330, 549
XspI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
Zsp2I	ATGCAT	1 cut(s)	777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.