Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0012569

Physical Location & Seq

Genomic Coordinates Forward (+)

38609024 .. 38620198

Gene Structure 11175 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG306300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGGGTTGTTCAAGAGCAAGGCTCGTGTGGTTTACGAGCCGGCGAGCCGAGTTGATGTCAGTCCGGCCAGTTCTGAGGTCTACTTGCGAGCCAATGTCAAAGCTCCTCGTATGGCTGGATTTCTGGCCAAATTGTTCGCCTGGTTTTTGGAATCGCGGATCTTTGGGACCATGTTGTTGTACATATTGAAAAGAGATAACTTGATCCATAAGTTGGTTACAAATGCTGAGCTCGAGGAATCACCAGTCTATGTTCCCTTACATCCTTTTGATGAGATTGAAGAGCAAGACGTCAAAAATATAGGGTCTGACTTGTCACCACCTGAATTGGTTCAACAGGCAATTGAATGTTTATCTGTATCCTTTGATAAGGAATTAAATGGTTCAAACCCTTCTTTCCGTTATTGGACAATATCAGATTATTCAAGAGCCTACAAGTCTGGAGAAATAACACCTAAATTGGTTGCAGAGCGTCTTATAGCTGCCATCCACGAGTCTTCTAATCCACCATTTAAGATGTCATTCTTTATTAATTACAACACTGAAGACATTCTAAAGCAAGCAACTGAATCAACTCTGCGGTATGAAAAAGGTGTGCCTATTTCAGCCCTAGATGGGATCCCAATTGCTATCAAGGATGAAATAGACTGCAGACCGTATCCAACTACTGGTGGAACGAAGTGGCTTCACAAATTCAGACCTTGTACAGATGACGCGTGCTGTGTCAAGTGCCTCCGACAATGTGGTGCCATACTTATTGGGAAAACCAATATGCATGAACTTGGAGCTGGTACAAGTGGGATAAATCCGCATTATGGGGCAACTAGAAATCCACACGACCCCAGCAAAATCTCTGGGGGTTCTTCTAGTGGATCTGCTGCAGTGGTCGCCTCTGGATTATGCCCTGTTGCTCTTGGTGTTGATGGAGGAGGATCTGTGCGGATGCCTGCAGCTCTTTGTGGTGTTGTAGGTTTAAAACCAACCTTTGGCCGAGTACCTCATTCTGGTGTTCTTCCTCTGAATTGGACGGTCGGGATGGTTGGAATACTAGCAGGCACTGTTGAGGATGCTTTAATAGTTTATGCGGCAATCATTGGCCAAATTTCGCCAAGTCAACCCACATTATCCCCCAAAGTATATTTTCCTCTACTGAACTCAGCAAAACCTATATCGCATATCAAATTGGCAAAATATGGTGAGTGGTTTAATGATTGCAGTGATGAGATCAGGGAATGCTGTACCAATGCCCTAGACAGGCTTCACAAGCAATATGGTTGGAAGACTGTAGAGGTGACCATACCAGAGATAGAGGTGATGCGGTTGGCACATTACTTGACAATTGGATCAGAGTGTAGCACTTCGCTTAGTTCTCACCTAGAAAAGTTGGATTTCAAAGAATTAGGGTGGGATGTAAGGGTAGGACTTGCTGTGTATGGTGCTTTCAGCAGTAAGGAGTATATACAGGCTCAGAGAATTAGTGGCTCTCCTATCGTTGCATATGTGACAAAACTGAACTTTTGTTTCGGGTTGACGTATAAGAGTATAAGAAGAGTGTCAAAAAAGGGTCAAAAAGGAGAAAGTCTACTACCCGGATCTAGCTGGAGCATCCTTCTGAGAAGATCCAATCCATATGGAGAGTCTACCAAAGGTGGCACAATGATCGCCATATCTACGGTTGGTAACTCTGGAATCTTCTCCATCCCGGCTGGCAACACTGTGTCACGTGCACTCGGCCAGTCATCTGTGGTCGGAGTCCCAAGTACACGAAAGATGGAAAAAACCTATCCATGTACTGTGTCATCATCAGTGGCAAAAATAGGAGCTCTAGTGATCATCTCAACAAATAGACAAGCGCAAGCCCAGATGTCAACTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0005886 GO:0006066 GO:0006576 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009308 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0016811 GO:0031090 GO:0034308 GO:0034641 GO:0042439 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044106 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0047412 GO:0050896 GO:0051704 GO:0051707 GO:0070291 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidase FPP

Protein Analysis

625

Amino Acids

67.81

Weight (kDa)

8.84

Isoelectric Point (pI)

46.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	180 - 458	9.1e-72	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012569)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25540 FvH4_1g25540 FvH4_1g25540 FvH4_1g25540
malus_domestica	MD15G1139000.v1.1
prunus_persica	Prupe.4G282000_v2.0.a1 Prupe.4G282000_v2.0.a1
pyrus_communis	pycom15g12560
rosa_chinensis	RchiOBHm_Chr2g0123671
rosa_laevigata	RLG00000018768
rosa_multiflora	Rmu_sc0004915.1_g000003
rosa_roxburghii	Rroxscaffold_2G00120450
rosa_rugosa	Rorug02G0247300
rosa_samantha	Rh2AG306300 Rh2BG314500 Rh2CG292700 Rh2DG330000 Rh7CG421300
rosa_wichuraiana	Rw2G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	294
AccB1I	GGYRCC	1 cut(s)	746
AccB7I	CCANNNNNTGG	3 cut(s)	214, 668, 986
AccI	GTMKAC	3 cut(s)	81, 1582, 1640
AccII	CGCG	2 cut(s)	157, 716
AciI	CCGC	6 cut(s)	157, 580, 809, 940, 1085, 1318
AclWI	GGATC	9 cut(s)	167, 199, 613, 626, 880, 940, 1351, 1600, 1614
AcoI	YGGCCR	5 cut(s)	66, 126, 988, 1096, 1732
AcsI	RAATTY	2 cut(s)	692, 1101
AcuI	CTGAAG	1 cut(s)	564
AcvI	CACGTG	1 cut(s)	1724
AcyI	GRCGYC	1 cut(s)	291
AfaI	GTAC	7 cut(s)	182, 706, 793, 996, 1240, 1762, 1792
AfiI	CCNNNNNNNGG	7 cut(s)	214, 615, 668, 815, 986, 1004, 1254
AflIII	ACRYGT	1 cut(s)	714
AgsI	TTSAA	8 cut(s)	13, 190, 281, 335, 347, 387, 426, 1393
AjnI	CCWGG	1 cut(s)	140
AluBI	AGCT	7 cut(s)	104, 232, 482, 788, 953, 1599, 1823
AluI	AGCT	7 cut(s)	104, 232, 482, 788, 953, 1599, 1823
Alw21I	GWGCWC	3 cut(s)	234, 1729, 1825
Alw44I	GTGCAC	1 cut(s)	1725
AlwI	GGATC	9 cut(s)	167, 199, 613, 626, 880, 940, 1351, 1600, 1614
AlwNI	CAGNNNCTG	1 cut(s)	1058
Ama87I	CYCGRG	1 cut(s)	233
AoxI	GGCC	5 cut(s)	66, 126, 988, 1096, 1732
ApaLI	GTGCAC	1 cut(s)	1725
ApeKI	GCWGC	3 cut(s)	482, 878, 950
ApoI	RAATTY	2 cut(s)	692, 1101
AseI	ATTAAT	1 cut(s)	531
Asp700I	GAANNNNTTC	2 cut(s)	330, 549
AspLEI	GCGC	1 cut(s)	1855
AspS9I	GGNCC	1 cut(s)	168
AsuC2I	CCSGG	2 cut(s)	1590, 1703
AsuHPI	GGTGA	6 cut(s)	234, 309, 1208, 1303, 1324, 1364
AvaI	CYCGRG	1 cut(s)	233
AvaII	GGWCC	1 cut(s)	168
BaeGI	GKGCMC	1 cut(s)	1729
BalI	TGGCCA	2 cut(s)	128, 1098
BamHI	GGATCC	1 cut(s)	618
BanI	GGYRCC	1 cut(s)	746
BanII	GRGCYC	2 cut(s)	234, 1825
BauI	CACGAG	2 cut(s)	24, 491
BbrPI	CACGTG	1 cut(s)	1724
BbsI	GAAGAC	3 cut(s)	489, 552, 1286
Bbv12I	GWGCWC	3 cut(s)	234, 1729, 1825
BbvI	GCAGC	3 cut(s)	469, 865, 962
BccI	CCATC	6 cut(s)	494, 608, 917, 1030, 1706, 1765
BcgI	CGANNNNNNTGC	2 cut(s)	1642, 1676
BciT130I	CCWGG	1 cut(s)	142
BciVI	GTATCC	2 cut(s)	370, 669
BclI	TGATCA	1 cut(s)	1830
BcnI	CCSGG	2 cut(s)	1590, 1703
BfaI	CTAG	8 cut(s)	611, 825, 867, 1049, 1250, 1376, 1596, 1826
BfmI	CTRYAG	4 cut(s)	649, 879, 948, 1284
BfuI	GTATCC	2 cut(s)	370, 669
BisI	GCNGC	4 cut(s)	483, 879, 951, 1086
BlpI	GCTNAGC	1 cut(s)	228
BlsI	GCNGC	4 cut(s)	484, 880, 952, 1087
Bme1390I	CCNGG	3 cut(s)	142, 1590, 1703
Bme18I	GGWCC	1 cut(s)	168
BmeT110I	CYCGRG	1 cut(s)	233
BmgT120I	GGNCC	1 cut(s)	168
BmiI	GGNNCC	3 cut(s)	169, 620, 748
BmrFI	CCNGG	3 cut(s)	142, 1590, 1703
BmsI	GCATC	4 cut(s)	933, 1057, 1305, 1614
BpiI	GAAGAC	3 cut(s)	489, 552, 1286
BplI	GAGNNNNNCTC	2 cut(s)	7, 39
BpmI	CTGGAG	2 cut(s)	462, 1621
Bpu1102I	GCTNAGC	1 cut(s)	228
BpuMI	CCSGG	2 cut(s)	1590, 1703
BsaAI	YACGTR	1 cut(s)	1724
BsaHI	GRCGYC	1 cut(s)	291
BsaXI	ACNNNNNCTCC	4 cut(s)	921, 951, 1812, 1842
Bsc4I	CCNNNNNNNGG	7 cut(s)	214, 615, 668, 815, 986, 1004, 1254
Bse118I	RCCGGY	1 cut(s)	40
Bse1I	ACTGG	4 cut(s)	69, 245, 673, 1735
BseBI	CCWGG	1 cut(s)	142
BseGI	GGATG	9 cut(s)	262, 486, 643, 948, 1041, 1072, 1414, 1605, 1698
BseLI	CCNNNNNNNGG	7 cut(s)	214, 615, 668, 815, 986, 1004, 1254
BseMII	CTCAG	5 cut(s)	66, 219, 1170, 1481, 1604
BseNI	ACTGG	4 cut(s)	69, 245, 673, 1735
BseRI	GAGGAG	2 cut(s)	96, 942
BseSI	GKGCMC	1 cut(s)	1729
BseXI	GCAGC	3 cut(s)	469, 865, 962
BseYI	CCCAGC	1 cut(s)	842
Bsh1236I	CGCG	2 cut(s)	157, 716
Bsh1285I	CGRYCG	1 cut(s)	1032
BshFI	GGCC	5 cut(s)	68, 128, 990, 1098, 1734
BshNI	GGYRCC	1 cut(s)	746
BsiEI	CGRYCG	1 cut(s)	1032
BsiHKAI	GWGCWC	3 cut(s)	234, 1729, 1825
BsiHKCI	CYCGRG	1 cut(s)	233
BsiSI	CCGG	4 cut(s)	41, 65, 1590, 1703
BslFI	GGGAC	2 cut(s)	181, 1739
BslI	CCNNNNNNNGG	7 cut(s)	214, 615, 668, 815, 986, 1004, 1254
BsmFI	GGGAC	2 cut(s)	181, 1739
BsmI	GAATGC	1 cut(s)	1238
BsnI	GGCC	5 cut(s)	68, 128, 990, 1098, 1734
BsoBI	CYCGRG	1 cut(s)	233
Bsp1286I	GDGCHC	3 cut(s)	234, 1729, 1825
Bsp1407I	TGTACA	2 cut(s)	180, 704
Bsp1720I	GCTNAGC	1 cut(s)	228
BspACI	CCGC	6 cut(s)	157, 580, 809, 940, 1085, 1318
BspANI	GGCC	5 cut(s)	68, 128, 990, 1098, 1734
BspCNI	CTCAG	5 cut(s)	67, 220, 1169, 1480, 1605
BspFNI	CGCG	2 cut(s)	157, 716
BspLI	GGNNCC	3 cut(s)	169, 620, 748
BspMAI	CTGCAG	3 cut(s)	653, 883, 952
BspPI	GGATC	9 cut(s)	167, 199, 613, 626, 880, 940, 1351, 1600, 1614
BspQI	GCTCTTC	1 cut(s)	276
BspT107I	GGYRCC	1 cut(s)	746
BsrFI	RCCGGY	1 cut(s)	40
BsrGI	TGTACA	2 cut(s)	180, 704
BsrI	ACTGG	4 cut(s)	69, 245, 673, 1735
BssAI	RCCGGY	1 cut(s)	40
BssNI	GRCGYC	1 cut(s)	291
BssSI	CACGAG	2 cut(s)	24, 491
Bst2BI	CACGAG	2 cut(s)	24, 491
Bst2UI	CCWGG	1 cut(s)	142
Bst4CI	ACNGT	7 cut(s)	657, 1030, 1060, 1285, 1675, 1717, 1795
Bst6I	CTCTTC	2 cut(s)	276, 1543
BstACI	GRCGYC	1 cut(s)	291
BstAUI	TGTACA	2 cut(s)	180, 704
BstBAI	YACGTR	1 cut(s)	1724
BstC8I	GCNNGC	9 cut(s)	42, 46, 90, 561, 718, 948, 1054, 1708, 1857
BstDEI	CTNAG	6 cut(s)	75, 228, 1156, 1364, 1467, 1613
BstEII	GGTNACC	1 cut(s)	1291
BstF5I	GGATG	9 cut(s)	262, 486, 643, 948, 1041, 1072, 1414, 1605, 1698
BstFNI	CGCG	2 cut(s)	157, 716
BstHHI	GCGC	1 cut(s)	1855
BstMCI	CGRYCG	1 cut(s)	1032
BstMWI	GCNNNNNNNGC	2 cut(s)	887, 1264
BstNI	CCWGG	1 cut(s)	142
BstPI	GGTNACC	1 cut(s)	1291
BstSCI	CCNGG	3 cut(s)	140, 1588, 1701
BstSFI	CTRYAG	4 cut(s)	649, 879, 948, 1284
BstSLI	GKGCMC	1 cut(s)	1729
BstUI	CGCG	2 cut(s)	157, 716
BstV1I	GCAGC	3 cut(s)	469, 865, 962
BstV2I	GAAGAC	3 cut(s)	489, 552, 1286
BstX2I	RGATCY	6 cut(s)	159, 618, 872, 932, 1592, 1619
BstYI	RGATCY	6 cut(s)	159, 618, 872, 932, 1592, 1619
BsuI	GTATCC	2 cut(s)	370, 669
BsuRI	GGCC	5 cut(s)	68, 128, 990, 1098, 1734
BtsCI	GGATG	9 cut(s)	262, 486, 643, 948, 1041, 1072, 1414, 1605, 1698
BtsI	GCAGTG	2 cut(s)	888, 1222
BtsIMutI	CAGTG	6 cut(s)	540, 888, 1056, 1222, 1713, 1812
Cac8I	GCNNGC	9 cut(s)	42, 46, 90, 561, 718, 948, 1054, 1708, 1857
CaiI	CAGNNNCTG	1 cut(s)	1058
CfoI	GCGC	1 cut(s)	1855
Cfr10I	RCCGGY	1 cut(s)	40
Cfr13I	GGNCC	1 cut(s)	168
CseI	GACGC	2 cut(s)	461, 722
Csp6I	GTAC	7 cut(s)	181, 705, 792, 995, 1239, 1761, 1791
CspCI	CAANNNNNGTGG	2 cut(s)	309, 344
CviAII	CATG	3 cut(s)	172, 776, 1788
CviQI	GTAC	7 cut(s)	181, 705, 792, 995, 1239, 1761, 1791
DdeI	CTNAG	6 cut(s)	75, 228, 1156, 1364, 1467, 1613
DraI	TTTAAA	1 cut(s)	975
EaeI	YGGCCR	5 cut(s)	66, 126, 988, 1096, 1732
Eam1104I	CTCTTC	2 cut(s)	276, 1543
EarI	CTCTTC	2 cut(s)	276, 1543
Ecl136II	GAGCTC	2 cut(s)	232, 1823
Eco24I	GRGCYC	2 cut(s)	234, 1825
Eco47I	GGWCC	1 cut(s)	168
Eco53kI	GAGCTC	2 cut(s)	232, 1823
Eco57I	CTGAAG	1 cut(s)	564
Eco72I	CACGTG	1 cut(s)	1724
Eco88I	CYCGRG	1 cut(s)	233
Eco91I	GGTNACC	1 cut(s)	1291
EcoICRI	GAGCTC	2 cut(s)	232, 1823
EcoO65I	GGTNACC	1 cut(s)	1291
EcoRII	CCWGG	1 cut(s)	140
EcoT22I	ATGCAT	1 cut(s)	777
EcoT38I	GRGCYC	2 cut(s)	234, 1825
FaeI	CATG	3 cut(s)	175, 779, 1791
FaqI	GGGAC	2 cut(s)	181, 1739
FatI	CATG	3 cut(s)	171, 775, 1787
FauNDI	CATATG	2 cut(s)	1498, 1630
FbaI	TGATCA	1 cut(s)	1830
FblI	GTMKAC	3 cut(s)	81, 1582, 1640
Fnu4HI	GCNGC	4 cut(s)	483, 879, 951, 1086
FokI	GGATG	9 cut(s)	249, 473, 650, 955, 1048, 1079, 1421, 1592, 1685
FriOI	GRGCYC	2 cut(s)	234, 1825
Fsp4HI	GCNGC	4 cut(s)	483, 879, 951, 1086
FspBI	CTAG	8 cut(s)	611, 825, 867, 1049, 1250, 1376, 1596, 1826
GlaI	GCGC	1 cut(s)	1854
GluI	GCNGC	4 cut(s)	483, 879, 951, 1086
GsaI	CCCAGC	1 cut(s)	846
GsuI	CTGGAG	2 cut(s)	462, 1621
HaeIII	GGCC	5 cut(s)	68, 128, 990, 1098, 1734
HapII	CCGG	4 cut(s)	41, 65, 1590, 1703
HgaI	GACGC	2 cut(s)	461, 722
HhaI	GCGC	1 cut(s)	1855
Hin1I	GRCGYC	1 cut(s)	291
Hin1II	CATG	3 cut(s)	175, 779, 1791
Hin6I	GCGC	1 cut(s)	1853
HinP1I	GCGC	1 cut(s)	1853
HincII	GTYRAC	3 cut(s)	1115, 1530, 1869
HindII	GTYRAC	3 cut(s)	1115, 1530, 1869
HinfI	GANTC	7 cut(s)	152, 239, 494, 569, 1637, 1689, 1752
HpaII	CCGG	4 cut(s)	41, 65, 1590, 1703
HphI	GGTGA	6 cut(s)	234, 309, 1208, 1303, 1324, 1364
Hpy166II	GTNNAC	9 cut(s)	34, 82, 1115, 1530, 1583, 1641, 1727, 1763, 1869
Hpy188III	TCNNGA	6 cut(s)	13, 426, 441, 894, 1033, 1686
Hpy8I	GTNNAC	9 cut(s)	34, 82, 1115, 1530, 1583, 1641, 1727, 1763, 1869
HpyAV	CCTTC	2 cut(s)	402, 1619
HpyCH4III	ACNGT	7 cut(s)	657, 1030, 1060, 1285, 1675, 1717, 1795
HpyCH4IV	ACGT	3 cut(s)	291, 1532, 1723
HpyCH4V	TGCA	8 cut(s)	467, 651, 775, 881, 950, 1215, 1496, 1727
HpyF10VI	GCNNNNNNNGC	2 cut(s)	887, 1264
HpyF3I	CTNAG	6 cut(s)	75, 228, 1156, 1364, 1467, 1613
HpySE526I	ACGT	3 cut(s)	291, 1532, 1723
Hsp92I	GRCGYC	1 cut(s)	291
Hsp92II	CATG	3 cut(s)	175, 779, 1791
HspAI	GCGC	1 cut(s)	1853
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
Ksp22I	TGATCA	1 cut(s)	1830
LguI	GCTCTTC	1 cut(s)	276
LmnI	GCTCC	4 cut(s)	109, 785, 1602, 1820
Lsp1109I	GCAGC	3 cut(s)	469, 865, 962
LweI	GCATC	4 cut(s)	933, 1057, 1305, 1614
MaeI	CTAG	8 cut(s)	611, 825, 867, 1049, 1250, 1376, 1596, 1826
MaeII	ACGT	3 cut(s)	291, 1532, 1723
MaeIII	GTNAC	6 cut(s)	217, 315, 1291, 1501, 1679, 1719
MboII	GAAGA	9 cut(s)	293, 489, 557, 855, 1004, 1291, 1560, 1629, 1684
MfeI	CAATTG	3 cut(s)	342, 624, 1338
MflI	RGATCY	6 cut(s)	159, 618, 872, 932, 1592, 1619
MhlI	GDGCHC	3 cut(s)	234, 1729, 1825
MlsI	TGGCCA	2 cut(s)	128, 1098
MluI	ACGCGT	1 cut(s)	714
MluNI	TGGCCA	2 cut(s)	128, 1098
MlyI	GAGTC	3 cut(s)	503, 1646, 1761
MmeI	TCCRAC	6 cut(s)	686, 760, 1021, 1256, 1365, 1729
Mox20I	TGGCCA	2 cut(s)	128, 1098
Mph1103I	ATGCAT	1 cut(s)	777
MroNI	GCCGGC	1 cut(s)	40
MroXI	GAANNNNTTC	2 cut(s)	330, 549
MscI	TGGCCA	2 cut(s)	128, 1098
MseI	TTAA	6 cut(s)	377, 513, 531, 974, 1073, 1206
MslI	CAYNNNNRTG	1 cut(s)	1005
Msp20I	TGGCCA	2 cut(s)	128, 1098
MspI	CCGG	4 cut(s)	41, 65, 1590, 1703
MspR9I	CCNGG	3 cut(s)	142, 1590, 1703
MunI	CAATTG	3 cut(s)	342, 624, 1338
Mva1269I	GAATGC	1 cut(s)	1238
MvaI	CCWGG	1 cut(s)	142
MvnI	CGCG	2 cut(s)	157, 716
MwoI	GCNNNNNNNGC	2 cut(s)	887, 1264
NaeI	GCCGGC	1 cut(s)	42
NciI	CCSGG	2 cut(s)	1590, 1703
NdeI	CATATG	2 cut(s)	1498, 1630
NgoMIV	GCCGGC	1 cut(s)	40
NlaIII	CATG	3 cut(s)	175, 779, 1791
NlaIV	GGNNCC	3 cut(s)	169, 620, 748
NmeAIII	GCCGAG	3 cut(s)	74, 1016, 1710
NmuCI	GTSAC	4 cut(s)	315, 1291, 1501, 1719
NsiI	ATGCAT	1 cut(s)	777
PaeR7I	CTCGAG	1 cut(s)	233
PciSI	GCTCTTC	1 cut(s)	276
PctI	GAATGC	1 cut(s)	1238
PdiI	GCCGGC	1 cut(s)	42
PdmI	GAANNNNTTC	2 cut(s)	330, 549
PfeI	GAWTC	4 cut(s)	152, 239, 569, 1689
PflMI	CCANNNNNTGG	3 cut(s)	214, 668, 986
PkrI	GCNGC	4 cut(s)	484, 880, 952, 1087
PleI	GAGTC	3 cut(s)	502, 1645, 1760
PmaCI	CACGTG	1 cut(s)	1724
PmlI	CACGTG	1 cut(s)	1724
PpsI	GAGTC	3 cut(s)	502, 1645, 1760
Ppu21I	YACGTR	1 cut(s)	1724
PshBI	ATTAAT	1 cut(s)	531
Psp124BI	GAGCTC	2 cut(s)	234, 1825
Psp6I	CCWGG	1 cut(s)	140
PspCI	CACGTG	1 cut(s)	1724
PspEI	GGTNACC	1 cut(s)	1291
PspFI	CCCAGC	1 cut(s)	842
PspGI	CCWGG	1 cut(s)	140
PspN4I	GGNNCC	3 cut(s)	169, 620, 748
PspPI	GGNCC	1 cut(s)	168
PspXI	VCTCGAGB	1 cut(s)	233
PstI	CTGCAG	3 cut(s)	653, 883, 952
PstNI	CAGNNNCTG	1 cut(s)	1058
PsuI	RGATCY	6 cut(s)	159, 618, 872, 932, 1592, 1619
RsaI	GTAC	7 cut(s)	182, 706, 793, 996, 1240, 1762, 1792
RsaNI	GTAC	7 cut(s)	181, 705, 792, 995, 1239, 1761, 1791
RseI	CAYNNNNRTG	1 cut(s)	1005
SacI	GAGCTC	2 cut(s)	234, 1825
SapI	GCTCTTC	1 cut(s)	276
SaqAI	TTAA	6 cut(s)	377, 513, 531, 974, 1073, 1206
SatI	GCNGC	4 cut(s)	483, 879, 951, 1086
Sau96I	GGNCC	1 cut(s)	168
SchI	GAGTC	3 cut(s)	503, 1646, 1761
ScrFI	CCNGG	3 cut(s)	142, 1590, 1703
SduI	GDGCHC	3 cut(s)	234, 1729, 1825
SfaNI	GCATC	4 cut(s)	933, 1057, 1305, 1614
SfcI	CTRYAG	4 cut(s)	649, 879, 948, 1284
Sfr274I	CTCGAG	1 cut(s)	233
SinI	GGWCC	1 cut(s)	168
SlaI	CTCGAG	1 cut(s)	233
SmiMI	CAYNNNNRTG	1 cut(s)	1005
SmlI	CTYRAG	1 cut(s)	233
SmoI	CTYRAG	1 cut(s)	233
SsiI	CCGC	6 cut(s)	157, 580, 809, 940, 1085, 1318
SspMI	CTAG	8 cut(s)	611, 825, 867, 1049, 1250, 1376, 1596, 1826
SstI	GAGCTC	2 cut(s)	234, 1825
StyD4I	CCNGG	3 cut(s)	140, 1588, 1701
TaaI	ACNGT	7 cut(s)	657, 1030, 1060, 1285, 1675, 1717, 1795
TaiI	ACGT	3 cut(s)	294, 1535, 1726
TaqI	TCGA	1 cut(s)	234
TatI	WGTACW	4 cut(s)	180, 704, 1760, 1790
TauI	GCSGC	1 cut(s)	1088
TfiI	GAWTC	4 cut(s)	152, 239, 569, 1689
Tru1I	TTAA	6 cut(s)	377, 513, 531, 974, 1073, 1206
Tru9I	TTAA	6 cut(s)	377, 513, 531, 974, 1073, 1206
TscAI	CASTG	6 cut(s)	547, 888, 1063, 1222, 1720, 1812
TseFI	GTSAC	4 cut(s)	315, 1291, 1501, 1719
TseI	GCWGC	3 cut(s)	482, 878, 950
Tsp45I	GTSAC	4 cut(s)	315, 1291, 1501, 1719
TspDTI	ATGAA	3 cut(s)	600, 654, 792
TspGWI	ACGGA	1 cut(s)	389
TspRI	CASTG	6 cut(s)	547, 888, 1063, 1222, 1720, 1812
Van91I	CCANNNNNTGG	3 cut(s)	214, 668, 986
VneI	GTGCAC	1 cut(s)	1725
VpaK11BI	GGWCC	1 cut(s)	168
VspI	ATTAAT	1 cut(s)	531
XapI	RAATTY	2 cut(s)	692, 1101
XhoI	CTCGAG	1 cut(s)	233
XmiI	GTMKAC	3 cut(s)	81, 1582, 1640
XmnI	GAANNNNTTC	2 cut(s)	330, 549
XspI	CTAG	8 cut(s)	611, 825, 867, 1049, 1250, 1376, 1596, 1826
ZraI	GACGTC	1 cut(s)	292
Zsp2I	ATGCAT	1 cut(s)	777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.