Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0012569

Physical Location & Seq

Genomic Coordinates Forward (+)

36265564 .. 36272446

Gene Structure 6883 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G024560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGGGTTGTTCAAGAGCGAGGCTCGTGTGGTTTACGAGCCGGCGAGCCGAGTTGATGTCAGTCCGGCCAGTTCTGAGGTCTACTTGCGAGCCAATGTCAAAGCTCCTCGTATGGCTGGATTTCTGGCCAAATTGTTCGCCTGGTTTTTGGAATCGCGGATCTTTGGGACCATGTTGTTGTACATATTGAAAAGAGATAACTTGATCCATAAGTTGGTTACAAATGCTGAGCTCGAGGAATCACCAGTCTATGTTCCCTTACATCCTTTTGATGAGATTGAAGAACAAGATGTCAAACATGTAGGGTCTGACTTGTCACCACCTGAATTGGTTCAACAGGCAATTGAATGTTTACCTGTATCCTTTGATAAGGAATTAAATGGTTCAAACTCTTCTTTCCGTTATTGGACAATATCAGATTATTCAAGAGCCTACAAGTCTGGAGAAATAACACCTAAATTGGTTGCAGAGCGTCTTATAGCTGCCATCCACGAGTCTTCTAATCCACCATTTAAGATGTCATTCTTTATTAATTACAACACTGAAGACATTCTAAAGCAAGCAACTGAATCAACTCTGCGGTATGAAAAAGGTGTGCCTATTTCAGCCCTAGATGGGATCCCAATTGCTATCAAGGATGAAATAGACTGCAGACCGTATCCAACTACTGGTGGAACGAAGTGGCTTCACAAATTCAGACCTTGTACAGATGACGCGTGCTGTGTCAAGTGCCTCCGACAATGTGGTGCCATACTTATTGGGAAAACCAATATGCATGAACTTGGAGCTGGTACAAGTGGGATAAATCCGCATTATGGGGCAACTAGAAATCCACACGACCCCAGAAAAATCTCTGGGGGTTCTTCTAGTGGATCTGCTGCAGTGGTCGCCTCTGGATTATGCCCTGTTGCTCTTGGTGTTGATGGAGGAGGATCTGTGCGGATGCCAGCAGCTCTTTGTGGTGTTGTAGGTTTAAAACCAACCTTTGGCCGAGTACCTCATTCTGGTGTTCTTCCTCTGAATTGGACGGTCGGGATGGTTGGAATACTAGCAGGCACTGTTGAGGATGCTTTAATAGTTTATGCGGCAATCATTGCCCAAATTTCGCCAAATCAACCCACATTATCCCCCAAAGTATATTTTCCTCTACTGAACTCAGCAAAACCTATATCGGATATCAAATTGGCAAAATATGGTGAGTGGTTTAATGATTGCAGTGATGAGATCAGGGAATGCTGTACCAATGCCCTAGACAGGCTTCACAAGCAATATGGTTGGAAGACTGTAGAGGTGACCATACCAGAGATAGAGGTGATGCGGTTGGCACATTACTTGACAATTGGATCAGAGTGTAGCACTTCGCTTAGCTCTCACCTAGAAAAGTTGGATTTCAAAGAATTAGGGTGGGATGTAAGGGTAGGACTTGCTGTGTATGGTGCTTTCAGCAGTAAGGAGTATATACAGGCTCAGAGAATTAGGAACCGTCAGATGCAATTTCACAAGAATTTGTTTTCTCATGCAGATGTCATTGTTTCCCCAACAGTAGGGGTAACTGCATACCCAATTTTGGATGATGCTCTCAAGACTGGAGAACTTGACTATATAAATGGAGCTGCACTTGTTCGATATTCCATATCCGGAAATTTTTTGGGACTACCAGCAGTGACTATTCCGGTTGGATACGATAAATCAGGTCTGCCAATTGGTCTTCAGTTGATTGGAAAACCATGGTCTGAACCAACATTAATTCACATAGCCTTTGCAGTGCAGAACCTTTATGTCGCTGATTGCAGAAAGCCCCAGGTCTATTATAATTTGCTGAATACAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0005886 GO:0006066 GO:0006576 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009308 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0016811 GO:0031090 GO:0034308 GO:0034641 GO:0042439 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044106 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0047412 GO:0050896 GO:0051704 GO:0051707 GO:0070291 GO:0071704 GO:0071944 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amidase FPP

Protein Analysis

610

Amino Acids

66.96

Weight (kDa)

6.58

Isoelectric Point (pI)

40.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	180 - 582	3.6e-90	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012569)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g25540 FvH4_1g25540 FvH4_1g25540 FvH4_1g25540
malus_domestica	MD15G1139000.v1.1
prunus_persica	Prupe.4G282000_v2.0.a1 Prupe.4G282000_v2.0.a1
pyrus_communis	pycom15g12560
rosa_chinensis	RchiOBHm_Chr2g0123671
rosa_laevigata	RLG00000018768
rosa_multiflora	Rmu_sc0004915.1_g000003
rosa_roxburghii	Rroxscaffold_2G00120450
rosa_rugosa	Rorug02G0247300
rosa_samantha	Rh2AG306300 Rh2BG314500 Rh2CG292700 Rh2DG330000 Rh7CG421300
rosa_wichuraiana	Rw2G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1812
AccB1I	GGYRCC	1 cut(s)	746
AccB7I	CCANNNNNTGG	3 cut(s)	214, 668, 986
AccI	GTMKAC	1 cut(s)	81
AccII	CGCG	2 cut(s)	157, 716
AccIII	TCCGGA	1 cut(s)	1637
AciI	CCGC	6 cut(s)	157, 580, 809, 940, 1085, 1318
AclWI	GGATC	7 cut(s)	167, 199, 613, 626, 880, 940, 1351
AcoI	YGGCCR	3 cut(s)	66, 126, 988
AcsI	RAATTY	4 cut(s)	692, 1101, 1504, 1642
AcuI	CTGAAG	2 cut(s)	564, 1694
AfaI	GTAC	5 cut(s)	182, 706, 793, 996, 1240
AfiI	CCNNNNNNNGG	9 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544, 1567
AflIII	ACRYGT	2 cut(s)	298, 714
AgsI	TTSAA	8 cut(s)	13, 190, 281, 335, 347, 387, 426, 1393
AjnI	CCWGG	2 cut(s)	140, 1800
AluBI	AGCT	7 cut(s)	104, 232, 482, 788, 953, 1368, 1613
AluI	AGCT	7 cut(s)	104, 232, 482, 788, 953, 1368, 1613
Alw21I	GWGCWC	1 cut(s)	234
AlwI	GGATC	7 cut(s)	167, 199, 613, 626, 880, 940, 1351
AlwNI	CAGNNNCTG	1 cut(s)	1058
Ama87I	CYCGRG	1 cut(s)	233
Aor13HI	TCCGGA	1 cut(s)	1637
AoxI	GGCC	3 cut(s)	66, 126, 988
ApeKI	GCWGC	4 cut(s)	482, 878, 950, 1613
ApoI	RAATTY	4 cut(s)	692, 1101, 1504, 1642
AseI	ATTAAT	2 cut(s)	531, 1745
Asp700I	GAANNNNTTC	2 cut(s)	330, 549
AspS9I	GGNCC	1 cut(s)	168
AsuHPI	GGTGA	6 cut(s)	234, 309, 1208, 1303, 1324, 1364
AvaI	CYCGRG	1 cut(s)	233
AvaII	GGWCC	1 cut(s)	168
BalI	TGGCCA	1 cut(s)	128
BamHI	GGATCC	1 cut(s)	618
BanI	GGYRCC	1 cut(s)	746
BanII	GRGCYC	1 cut(s)	234
BauI	CACGAG	2 cut(s)	24, 491
BbsI	GAAGAC	4 cut(s)	489, 552, 1286, 1700
Bbv12I	GWGCWC	1 cut(s)	234
BbvI	GCAGC	4 cut(s)	469, 865, 962, 1600
BccI	CCATC	4 cut(s)	494, 608, 917, 1030
BciT130I	CCWGG	2 cut(s)	142, 1802
BciVI	GTATCC	3 cut(s)	370, 669, 1673
BfaI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
BfmI	CTRYAG	3 cut(s)	649, 879, 1284
BfuI	GTATCC	3 cut(s)	370, 669, 1673
BisI	GCNGC	5 cut(s)	483, 879, 951, 1086, 1614
BlpI	GCTNAGC	2 cut(s)	228, 1364
BlsI	GCNGC	5 cut(s)	484, 880, 952, 1087, 1615
Bme1390I	CCNGG	2 cut(s)	142, 1802
Bme18I	GGWCC	1 cut(s)	168
BmeT110I	CYCGRG	1 cut(s)	233
BmgT120I	GGNCC	1 cut(s)	168
BmiI	GGNNCC	4 cut(s)	169, 620, 748, 1481
BmrFI	CCNGG	2 cut(s)	142, 1802
BmsI	GCATC	5 cut(s)	933, 1057, 1305, 1479, 1564
BpiI	GAAGAC	4 cut(s)	489, 552, 1286, 1700
BplI	GAGNNNNNCTC	2 cut(s)	7, 39
BpmI	CTGGAG	2 cut(s)	462, 1608
Bpu1102I	GCTNAGC	2 cut(s)	228, 1364
BpuEI	CTTGAG	1 cut(s)	1565
BsaJI	CCNNGG	2 cut(s)	1727, 1800
BsaWI	WCCGGW	2 cut(s)	1637, 1672
BsaXI	ACNNNNNCTCC	2 cut(s)	921, 951
Bsc4I	CCNNNNNNNGG	9 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544, 1567
Bse118I	RCCGGY	1 cut(s)	40
Bse1I	ACTGG	4 cut(s)	69, 245, 673, 1591
Bse3DI	GCAATG	1 cut(s)	1092
BseAI	TCCGGA	1 cut(s)	1637
BseBI	CCWGG	2 cut(s)	142, 1802
BseDI	CCNNGG	2 cut(s)	1727, 1800
BseGI	GGATG	8 cut(s)	262, 486, 643, 948, 1041, 1072, 1414, 1576
BseLI	CCNNNNNNNGG	9 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544, 1567
BseMI	GCAATG	1 cut(s)	1092
BseMII	CTCAG	4 cut(s)	66, 219, 1170, 1481
BseNI	ACTGG	4 cut(s)	69, 245, 673, 1591
BseRI	GAGGAG	2 cut(s)	96, 942
BseXI	GCAGC	4 cut(s)	469, 865, 962, 1600
BsgI	GTGCAG	2 cut(s)	1599, 1787
Bsh1236I	CGCG	2 cut(s)	157, 716
Bsh1285I	CGRYCG	1 cut(s)	1032
BshFI	GGCC	3 cut(s)	68, 128, 990
BshNI	GGYRCC	1 cut(s)	746
BsiEI	CGRYCG	1 cut(s)	1032
BsiHKAI	GWGCWC	1 cut(s)	234
BsiHKCI	CYCGRG	1 cut(s)	233
BsiSI	CCGG	4 cut(s)	41, 65, 1638, 1673
BslFI	GGGAC	2 cut(s)	181, 1665
BslI	CCNNNNNNNGG	9 cut(s)	214, 615, 668, 815, 986, 1004, 1254, 1544, 1567
BsmFI	GGGAC	2 cut(s)	181, 1665
BsmI	GAATGC	1 cut(s)	1238
BsnI	GGCC	3 cut(s)	68, 128, 990
BsoBI	CYCGRG	1 cut(s)	233
Bsp1286I	GDGCHC	1 cut(s)	234
Bsp13I	TCCGGA	1 cut(s)	1637
Bsp1407I	TGTACA	2 cut(s)	180, 704
Bsp143I	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
Bsp1720I	GCTNAGC	2 cut(s)	228, 1364
Bsp19I	CCATGG	1 cut(s)	1727
BspACI	CCGC	6 cut(s)	157, 580, 809, 940, 1085, 1318
BspANI	GGCC	3 cut(s)	68, 128, 990
BspCNI	CTCAG	4 cut(s)	67, 220, 1169, 1480
BspEI	TCCGGA	1 cut(s)	1637
BspFNI	CGCG	2 cut(s)	157, 716
BspLI	GGNNCC	4 cut(s)	169, 620, 748, 1481
BspMAI	CTGCAG	2 cut(s)	653, 883
BspPI	GGATC	7 cut(s)	167, 199, 613, 626, 880, 940, 1351
BspT107I	GGYRCC	1 cut(s)	746
BsrDI	GCAATG	1 cut(s)	1092
BsrFI	RCCGGY	1 cut(s)	40
BsrGI	TGTACA	2 cut(s)	180, 704
BsrI	ACTGG	4 cut(s)	69, 245, 673, 1591
BssAI	RCCGGY	1 cut(s)	40
BssECI	CCNNGG	2 cut(s)	1727, 1800
BssMI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
BssSI	CACGAG	2 cut(s)	24, 491
BssT1I	CCWWGG	1 cut(s)	1727
Bst2BI	CACGAG	2 cut(s)	24, 491
Bst2UI	CCWGG	2 cut(s)	142, 1802
Bst4CI	ACNGT	6 cut(s)	657, 1030, 1060, 1285, 1484, 1543
Bst6I	CTCTTC	1 cut(s)	397
BstAPI	GCANNNNNTGC	1 cut(s)	1094
BstAUI	TGTACA	2 cut(s)	180, 704
BstC8I	GCNNGC	7 cut(s)	42, 46, 90, 561, 718, 948, 1054
BstDEI	CTNAG	5 cut(s)	75, 228, 1156, 1364, 1467
BstDSI	CCRYGG	1 cut(s)	1727
BstEII	GGTNACC	1 cut(s)	1291
BstF5I	GGATG	8 cut(s)	262, 486, 643, 948, 1041, 1072, 1414, 1576
BstFNI	CGCG	2 cut(s)	157, 716
BstKTI	GATC	7 cut(s)	162, 207, 621, 875, 935, 1227, 1346
BstMBI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
BstMCI	CGRYCG	1 cut(s)	1032
BstMWI	GCNNNNNNNGC	3 cut(s)	887, 1094, 1264
BstNI	CCWGG	2 cut(s)	142, 1802
BstNSI	RCATGY	1 cut(s)	302
BstPI	GGTNACC	1 cut(s)	1291
BstSCI	CCNGG	2 cut(s)	140, 1800
BstSFI	CTRYAG	3 cut(s)	649, 879, 1284
BstUI	CGCG	2 cut(s)	157, 716
BstV1I	GCAGC	4 cut(s)	469, 865, 962, 1600
BstV2I	GAAGAC	4 cut(s)	489, 552, 1286, 1700
BstX2I	RGATCY	4 cut(s)	159, 618, 872, 932
BstYI	RGATCY	4 cut(s)	159, 618, 872, 932
BsuI	GTATCC	3 cut(s)	370, 669, 1673
BsuRI	GGCC	3 cut(s)	68, 128, 990
BtgI	CCRYGG	1 cut(s)	1727
BtsCI	GGATG	8 cut(s)	262, 486, 643, 948, 1041, 1072, 1414, 1576
BtsI	GCAGTG	4 cut(s)	888, 1222, 1668, 1770
BtsIMutI	CAGTG	6 cut(s)	540, 888, 1056, 1222, 1668, 1770
Cac8I	GCNNGC	7 cut(s)	42, 46, 90, 561, 718, 948, 1054
CaiI	CAGNNNCTG	1 cut(s)	1058
Cfr10I	RCCGGY	1 cut(s)	40
Cfr13I	GGNCC	1 cut(s)	168
CseI	GACGC	2 cut(s)	461, 722
Csp6I	GTAC	5 cut(s)	181, 705, 792, 995, 1239
CspCI	CAANNNNNGTGG	2 cut(s)	309, 344
CviAII	CATG	5 cut(s)	172, 299, 776, 1517, 1728
CviQI	GTAC	5 cut(s)	181, 705, 792, 995, 1239
DdeI	CTNAG	5 cut(s)	75, 228, 1156, 1364, 1467
DpnI	GATC	7 cut(s)	161, 206, 620, 874, 934, 1226, 1345
DpnII	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
DraI	TTTAAA	1 cut(s)	975
EaeI	YGGCCR	3 cut(s)	66, 126, 988
Eam1104I	CTCTTC	1 cut(s)	397
EarI	CTCTTC	1 cut(s)	397
Ecl136II	GAGCTC	1 cut(s)	232
Eco130I	CCWWGG	1 cut(s)	1727
Eco24I	GRGCYC	1 cut(s)	234
Eco32I	GATATC	1 cut(s)	1177
Eco47I	GGWCC	1 cut(s)	168
Eco53kI	GAGCTC	1 cut(s)	232
Eco57I	CTGAAG	2 cut(s)	564, 1694
Eco88I	CYCGRG	1 cut(s)	233
Eco91I	GGTNACC	1 cut(s)	1291
EcoICRI	GAGCTC	1 cut(s)	232
EcoO65I	GGTNACC	1 cut(s)	1291
EcoRII	CCWGG	2 cut(s)	140, 1800
EcoRV	GATATC	1 cut(s)	1177
EcoT14I	CCWWGG	1 cut(s)	1727
EcoT22I	ATGCAT	1 cut(s)	777
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	1727
FaeI	CATG	5 cut(s)	175, 302, 779, 1520, 1731
FaqI	GGGAC	2 cut(s)	181, 1665
FatI	CATG	5 cut(s)	171, 298, 775, 1516, 1727
FblI	GTMKAC	1 cut(s)	81
Fnu4HI	GCNGC	5 cut(s)	483, 879, 951, 1086, 1614
FokI	GGATG	8 cut(s)	249, 473, 650, 955, 1048, 1079, 1421, 1583
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	5 cut(s)	483, 879, 951, 1086, 1614
FspBI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
GluI	GCNGC	5 cut(s)	483, 879, 951, 1086, 1614
GsuI	CTGGAG	2 cut(s)	462, 1608
HaeIII	GGCC	3 cut(s)	68, 128, 990
HapII	CCGG	4 cut(s)	41, 65, 1638, 1673
HgaI	GACGC	2 cut(s)	461, 722
Hin1II	CATG	5 cut(s)	175, 302, 779, 1520, 1731
HinfI	GANTC	4 cut(s)	152, 239, 494, 569
HpaII	CCGG	4 cut(s)	41, 65, 1638, 1673
HphI	GGTGA	6 cut(s)	234, 309, 1208, 1303, 1324, 1364
Hpy166II	GTNNAC	3 cut(s)	34, 82, 353
Hpy188III	TCNNGA	7 cut(s)	13, 426, 441, 894, 1033, 1582, 1638
Hpy8I	GTNNAC	3 cut(s)	34, 82, 353
HpyCH4III	ACNGT	6 cut(s)	657, 1030, 1060, 1285, 1484, 1543
HpyF10VI	GCNNNNNNNGC	3 cut(s)	887, 1094, 1264
HpyF3I	CTNAG	5 cut(s)	75, 228, 1156, 1364, 1467
Hsp92II	CATG	5 cut(s)	175, 302, 779, 1520, 1731
Kpn2I	TCCGGA	1 cut(s)	1637
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
Kzo9I	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
LmnI	GCTCC	3 cut(s)	109, 785, 1610
Lsp1109I	GCAGC	4 cut(s)	469, 865, 962, 1600
LweI	GCATC	5 cut(s)	933, 1057, 1305, 1479, 1564
MaeI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
MaeIII	GTNAC	5 cut(s)	217, 315, 1291, 1549, 1663
MalI	GATC	7 cut(s)	161, 206, 620, 874, 934, 1226, 1345
MboI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
MboII	GAAGA	8 cut(s)	293, 384, 489, 557, 855, 1004, 1291, 1700
MfeI	CAATTG	4 cut(s)	342, 624, 1338, 1701
MflI	RGATCY	4 cut(s)	159, 618, 872, 932
MhlI	GDGCHC	1 cut(s)	234
MlsI	TGGCCA	1 cut(s)	128
MluI	ACGCGT	1 cut(s)	714
MluNI	TGGCCA	1 cut(s)	128
MlyI	GAGTC	1 cut(s)	503
MmeI	TCCRAC	6 cut(s)	686, 760, 1021, 1256, 1365, 1657
Mox20I	TGGCCA	1 cut(s)	128
Mph1103I	ATGCAT	1 cut(s)	777
MroI	TCCGGA	1 cut(s)	1637
MroNI	GCCGGC	1 cut(s)	40
MroXI	GAANNNNTTC	2 cut(s)	330, 549
MscI	TGGCCA	1 cut(s)	128
MseI	TTAA	8 cut(s)	377, 513, 531, 974, 1073, 1206, 1745, 1831
MslI	CAYNNNNRTG	2 cut(s)	1005, 1521
Msp20I	TGGCCA	1 cut(s)	128
MspI	CCGG	4 cut(s)	41, 65, 1638, 1673
MspR9I	CCNGG	2 cut(s)	142, 1802
MunI	CAATTG	4 cut(s)	342, 624, 1338, 1701
Mva1269I	GAATGC	1 cut(s)	1238
MvaI	CCWGG	2 cut(s)	142, 1802
MvnI	CGCG	2 cut(s)	157, 716
MwoI	GCNNNNNNNGC	3 cut(s)	887, 1094, 1264
NaeI	GCCGGC	1 cut(s)	42
NcoI	CCATGG	1 cut(s)	1727
NdeII	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
NgoMIV	GCCGGC	1 cut(s)	40
NlaIII	CATG	5 cut(s)	175, 302, 779, 1520, 1731
NlaIV	GGNNCC	4 cut(s)	169, 620, 748, 1481
NmeAIII	GCCGAG	2 cut(s)	74, 1016
NmuCI	GTSAC	3 cut(s)	315, 1291, 1663
NsiI	ATGCAT	1 cut(s)	777
NspI	RCATGY	1 cut(s)	302
PaeR7I	CTCGAG	1 cut(s)	233
PciI	ACATGT	1 cut(s)	298
PctI	GAATGC	1 cut(s)	1238
PdiI	GCCGGC	1 cut(s)	42
PdmI	GAANNNNTTC	2 cut(s)	330, 549
PfeI	GAWTC	3 cut(s)	152, 239, 569
PflMI	CCANNNNNTGG	3 cut(s)	214, 668, 986
PkrI	GCNGC	5 cut(s)	484, 880, 952, 1087, 1615
PleI	GAGTC	1 cut(s)	502
PpsI	GAGTC	1 cut(s)	502
PscI	ACATGT	1 cut(s)	298
PshBI	ATTAAT	2 cut(s)	531, 1745
PsiI	TTATAA	1 cut(s)	1812
Psp124BI	GAGCTC	1 cut(s)	234
Psp6I	CCWGG	2 cut(s)	140, 1800
PspEI	GGTNACC	1 cut(s)	1291
PspGI	CCWGG	2 cut(s)	140, 1800
PspN4I	GGNNCC	4 cut(s)	169, 620, 748, 1481
PspPI	GGNCC	1 cut(s)	168
PspXI	VCTCGAGB	1 cut(s)	233
PstI	CTGCAG	2 cut(s)	653, 883
PstNI	CAGNNNCTG	1 cut(s)	1058
PsuI	RGATCY	4 cut(s)	159, 618, 872, 932
RsaI	GTAC	5 cut(s)	182, 706, 793, 996, 1240
RsaNI	GTAC	5 cut(s)	181, 705, 792, 995, 1239
RseI	CAYNNNNRTG	2 cut(s)	1005, 1521
SacI	GAGCTC	1 cut(s)	234
SaqAI	TTAA	8 cut(s)	377, 513, 531, 974, 1073, 1206, 1745, 1831
SatI	GCNGC	5 cut(s)	483, 879, 951, 1086, 1614
Sau3AI	GATC	7 cut(s)	159, 204, 618, 872, 932, 1224, 1343
Sau96I	GGNCC	1 cut(s)	168
SchI	GAGTC	1 cut(s)	503
ScrFI	CCNGG	2 cut(s)	142, 1802
SduI	GDGCHC	1 cut(s)	234
SfaNI	GCATC	5 cut(s)	933, 1057, 1305, 1479, 1564
SfcI	CTRYAG	3 cut(s)	649, 879, 1284
Sfr274I	CTCGAG	1 cut(s)	233
SinI	GGWCC	1 cut(s)	168
SlaI	CTCGAG	1 cut(s)	233
SmiMI	CAYNNNNRTG	2 cut(s)	1005, 1521
SmlI	CTYRAG	2 cut(s)	233, 1580
SmoI	CTYRAG	2 cut(s)	233, 1580
SsiI	CCGC	6 cut(s)	157, 580, 809, 940, 1085, 1318
SspMI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
SstI	GAGCTC	1 cut(s)	234
StyD4I	CCNGG	2 cut(s)	140, 1800
StyI	CCWWGG	1 cut(s)	1727
TaaI	ACNGT	6 cut(s)	657, 1030, 1060, 1285, 1484, 1543
TaqI	TCGA	2 cut(s)	234, 1624
TatI	WGTACW	2 cut(s)	180, 704
TauI	GCSGC	1 cut(s)	1088
TfiI	GAWTC	3 cut(s)	152, 239, 569
Tru1I	TTAA	8 cut(s)	377, 513, 531, 974, 1073, 1206, 1745, 1831
Tru9I	TTAA	8 cut(s)	377, 513, 531, 974, 1073, 1206, 1745, 1831
TscAI	CASTG	6 cut(s)	547, 888, 1063, 1222, 1668, 1770
TseFI	GTSAC	3 cut(s)	315, 1291, 1663
TseI	GCWGC	4 cut(s)	482, 878, 950, 1613
Tsp45I	GTSAC	3 cut(s)	315, 1291, 1663
TspDTI	ATGAA	3 cut(s)	600, 654, 792
TspGWI	ACGGA	1 cut(s)	389
TspRI	CASTG	6 cut(s)	547, 888, 1063, 1222, 1668, 1770
Van91I	CCANNNNNTGG	3 cut(s)	214, 668, 986
VpaK11BI	GGWCC	1 cut(s)	168
VspI	ATTAAT	2 cut(s)	531, 1745
XapI	RAATTY	4 cut(s)	692, 1101, 1504, 1642
XceI	RCATGY	1 cut(s)	302
XhoI	CTCGAG	1 cut(s)	233
XmiI	GTMKAC	1 cut(s)	81
XmnI	GAANNNNTTC	2 cut(s)	330, 549
XspI	CTAG	6 cut(s)	611, 825, 867, 1049, 1250, 1376
Zsp2I	ATGCAT	1 cut(s)	777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.