Rh2DG330000

Fatty acid amide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012569
Physical Location & Seq
Forward (+)
41869072 .. 41872937
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG330000.1

Sequence Viewer

Length: 1167 bp
ATGGGGTTGTTCAAGAGCAAGGCTCGTGTGGTTTACGAGCCGGCGAGCCGAGTTGATGTCAGTCCGGCCAGTTCTGAGGTCTACTTGCGAGCCAATGTCAAAGCTCCTCGTATGGCTGGATTTCTGGCCAAATTGTTCGCCTGGTTTTTGGAATCGCGGATCTTTGGGACCATGTTGTTGTACATATTGAAAAGAGATAACTTGATCCATAAGTTGGTTACAAATGCTGAGCTCGAGGAATCACCAGTCTATGTTCCCTTACATCCTTTTGATGAGATTGAAGAGCAAGACGTCAAAAATATAGGGTCTGACTTGTCACCACCTGAATTGGTTCAACAGGCAATTGAATGTTTATCTGTATCCTTTGATAAGGAATTAAATGGTTCAAACCCTTCTTTCCGTTATTGGACAATATCAGATTATTCAAGAGCCTACAAGTCTGGAGAAATAACACCTAAATTGGTTGCAGAGCGTCTTATAGCTGCCATCCACGAGTCTTCTAATCCACCATTTAAGATGTCATTCTTTATTAATTACAACACTGAAGACATTCTAAAGCAAGCAACTGAATCAACTCTGCGGTATGAAAAAGGTGTGCCTATTTCAGCCCTAGATGGGATCCCAATTGCTATCAAGGATGAAATAGACTGCAGACCGTATCCAACTACTGGTGGAACGAAGTGGCTTCACAAATTCAGACCTTGTACAGATGACGCGTGCTGTGTCAAGTGCCTCCGACAATGTGGTGCCATACTTATTGGGAAAACCAATATGCATGAACTTGGAGCTGGTACAAGTGGGATAAATCCGCATTATGGGGCAACTAGAAATCCACACGACCCCAGCAAAATCTCTGGGGGTTCTTCTAGTGGATCTGCTGCAGTGGTCGCCTCTGGATTATGCCCTGTTGCTCTTGGTGTTGATGGAGGAGGATCTGTGCGGATGCCTGCAGCTCTTTGTGGTGTTGTAGGTTTAAAACCAACCTTTGGCCGAGTACCTCATTCTGGTGTTCTTCCTCTGAATTGGACGGTCGGGATGGTTGGAATACTAGCAGGCACTGTTGAGGATGCTTTAATAGTTTATGCGGCAATCATTGGCCAAATTTCGCCAAGTCAACCCACATTATCCGTAAGTTTGACATCATGTCAACAAATCGTTACCAGATAA

Protein Analysis

388

Amino Acids

41.89

Weight (kDa)

7.06

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 180 - 372 1.4e-66 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012569)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25540 FvH4_1g25540 FvH4_1g25540 FvH4_1g25540
malus_domestica MD15G1139000.v1.1
prunus_persica Prupe.4G282000_v2.0.a1 Prupe.4G282000_v2.0.a1
pyrus_communis pycom15g12560
rosa_chinensis RchiOBHm_Chr2g0123671
rosa_laevigata RLG00000018768
rosa_multiflora Rmu_sc0004915.1_g000003
rosa_roxburghii Rroxscaffold_2G00120450
rosa_rugosa Rorug02G0247300
rosa_samantha Rh2AG306300 Rh2BG314500 Rh2CG292700 Rh2DG330000 Rh7CG421300
rosa_wichuraiana Rw2G024560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 746
AccB7I CCANNNNNTGG 3 cut(s) 214, 668, 986
AccI GTMKAC 1 cut(s) 81
AccII CGCG 2 cut(s) 157, 716
AciI CCGC 5 cut(s) 157, 580, 809, 940, 1085
AclWI GGATC 6 cut(s) 167, 199, 613, 626, 880, 940
AcoI YGGCCR 4 cut(s) 66, 126, 988, 1096
AcsI RAATTY 2 cut(s) 692, 1101
AcuI CTGAAG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 4 cut(s) 182, 706, 793, 996
AfiI CCNNNNNNNGG 6 cut(s) 214, 615, 668, 815, 986, 1004
AflIII ACRYGT 1 cut(s) 714
AgsI TTSAA 7 cut(s) 13, 190, 281, 335, 347, 387, 426
AhdI GACNNNNNGTC 1 cut(s) 1143
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 5 cut(s) 104, 232, 482, 788, 953
AluI AGCT 5 cut(s) 104, 232, 482, 788, 953
Alw21I GWGCWC 1 cut(s) 234
AlwI GGATC 6 cut(s) 167, 199, 613, 626, 880, 940
AlwNI CAGNNNCTG 1 cut(s) 1058
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 4 cut(s) 66, 126, 988, 1096
ApeKI GCWGC 3 cut(s) 482, 878, 950
ApoI RAATTY 2 cut(s) 692, 1101
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 2 cut(s) 330, 549
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 234, 309
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 1 cut(s) 168
BalI TGGCCA 2 cut(s) 128, 1098
BamHI GGATCC 1 cut(s) 618
BanI GGYRCC 1 cut(s) 746
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 2 cut(s) 24, 491
BbsI GAAGAC 2 cut(s) 489, 552
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 469, 865, 962
BccI CCATC 4 cut(s) 494, 608, 917, 1030
BciT130I CCWGG 1 cut(s) 142
BciVI GTATCC 2 cut(s) 370, 669
BfaI CTAG 4 cut(s) 611, 825, 867, 1049
BfmI CTRYAG 3 cut(s) 649, 879, 948
BfuI GTATCC 2 cut(s) 370, 669
BisI GCNGC 4 cut(s) 483, 879, 951, 1086
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 4 cut(s) 484, 880, 952, 1087
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 168
BmeRI GACNNNNNGTC 1 cut(s) 1143
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 3 cut(s) 169, 620, 748
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 933, 1057
BpiI GAAGAC 2 cut(s) 489, 552
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 462
Bpu1102I GCTNAGC 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 291
BsaXI ACNNNNNCTCC 2 cut(s) 921, 951
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 615, 668, 815, 986, 1004
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 69, 245, 673
BseBI CCWGG 1 cut(s) 142
BseGI GGATG 6 cut(s) 262, 486, 643, 948, 1041, 1072
BseLI CCNNNNNNNGG 6 cut(s) 214, 615, 668, 815, 986, 1004
BseMII CTCAG 2 cut(s) 66, 219
BseNI ACTGG 3 cut(s) 69, 245, 673
BseRI GAGGAG 2 cut(s) 96, 942
BseXI GCAGC 3 cut(s) 469, 865, 962
BseYI CCCAGC 1 cut(s) 842
Bsh1236I CGCG 2 cut(s) 157, 716
Bsh1285I CGRYCG 1 cut(s) 1032
BshFI GGCC 4 cut(s) 68, 128, 990, 1098
BshNI GGYRCC 1 cut(s) 746
BsiEI CGRYCG 1 cut(s) 1032
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 2 cut(s) 41, 65
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 6 cut(s) 214, 615, 668, 815, 986, 1004
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 4 cut(s) 68, 128, 990, 1098
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 234
Bsp1407I TGTACA 2 cut(s) 180, 704
Bsp143I GATC 5 cut(s) 159, 204, 618, 872, 932
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 5 cut(s) 157, 580, 809, 940, 1085
BspANI GGCC 4 cut(s) 68, 128, 990, 1098
BspCNI CTCAG 2 cut(s) 67, 220
BspFNI CGCG 2 cut(s) 157, 716
BspLI GGNNCC 3 cut(s) 169, 620, 748
BspMAI CTGCAG 3 cut(s) 653, 883, 952
BspPI GGATC 6 cut(s) 167, 199, 613, 626, 880, 940
BspQI GCTCTTC 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 746
BsrFI RCCGGY 1 cut(s) 40
BsrGI TGTACA 2 cut(s) 180, 704
BsrI ACTGG 3 cut(s) 69, 245, 673
BssAI RCCGGY 1 cut(s) 40
BssMI GATC 5 cut(s) 159, 204, 618, 872, 932
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 2 cut(s) 24, 491
Bst2BI CACGAG 2 cut(s) 24, 491
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 3 cut(s) 657, 1030, 1060
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 291
BstAUI TGTACA 2 cut(s) 180, 704
BstC8I GCNNGC 7 cut(s) 42, 46, 90, 561, 718, 948, 1054
BstDEI CTNAG 2 cut(s) 75, 228
BstF5I GGATG 6 cut(s) 262, 486, 643, 948, 1041, 1072
BstFNI CGCG 2 cut(s) 157, 716
BstKTI GATC 5 cut(s) 162, 207, 621, 875, 935
BstMBI GATC 5 cut(s) 159, 204, 618, 872, 932
BstMCI CGRYCG 1 cut(s) 1032
BstMWI GCNNNNNNNGC 1 cut(s) 887
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 3 cut(s) 649, 879, 948
BstUI CGCG 2 cut(s) 157, 716
BstV1I GCAGC 3 cut(s) 469, 865, 962
BstV2I GAAGAC 2 cut(s) 489, 552
BstX2I RGATCY 4 cut(s) 159, 618, 872, 932
BstYI RGATCY 4 cut(s) 159, 618, 872, 932
BsuI GTATCC 2 cut(s) 370, 669
BsuRI GGCC 4 cut(s) 68, 128, 990, 1098
BtsCI GGATG 6 cut(s) 262, 486, 643, 948, 1041, 1072
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 3 cut(s) 540, 888, 1056
Cac8I GCNNGC 7 cut(s) 42, 46, 90, 561, 718, 948, 1054
CaiI CAGNNNCTG 1 cut(s) 1058
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 168
CseI GACGC 2 cut(s) 461, 722
Csp6I GTAC 4 cut(s) 181, 705, 792, 995
CspCI CAANNNNNGTGG 2 cut(s) 309, 344
CviAII CATG 3 cut(s) 172, 776, 1143
CviQI GTAC 4 cut(s) 181, 705, 792, 995
DdeI CTNAG 2 cut(s) 75, 228
DpnI GATC 5 cut(s) 161, 206, 620, 874, 934
DpnII GATC 5 cut(s) 159, 204, 618, 872, 932
DraI TTTAAA 1 cut(s) 975
DriI GACNNNNNGTC 1 cut(s) 1143
EaeI YGGCCR 4 cut(s) 66, 126, 988, 1096
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 1143
EarI CTCTTC 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 140
EcoT22I ATGCAT 1 cut(s) 777
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 3 cut(s) 175, 779, 1146
FaqI GGGAC 1 cut(s) 181
FatI CATG 3 cut(s) 171, 775, 1142
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 483, 879, 951, 1086
FokI GGATG 6 cut(s) 249, 473, 650, 955, 1048, 1079
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 4 cut(s) 483, 879, 951, 1086
FspBI CTAG 4 cut(s) 611, 825, 867, 1049
GluI GCNGC 4 cut(s) 483, 879, 951, 1086
GsaI CCCAGC 1 cut(s) 846
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 4 cut(s) 68, 128, 990, 1098
HapII CCGG 2 cut(s) 41, 65
HgaI GACGC 2 cut(s) 461, 722
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 3 cut(s) 175, 779, 1146
HincII GTYRAC 2 cut(s) 1115, 1148
HindII GTYRAC 2 cut(s) 1115, 1148
HinfI GANTC 4 cut(s) 152, 239, 494, 569
HpaII CCGG 2 cut(s) 41, 65
HphI GGTGA 2 cut(s) 234, 309
Hpy166II GTNNAC 4 cut(s) 34, 82, 1115, 1148
Hpy188I TCNGA 6 cut(s) 76, 310, 418, 698, 737, 1020
Hpy188III TCNNGA 5 cut(s) 13, 426, 441, 894, 1033
Hpy8I GTNNAC 4 cut(s) 34, 82, 1115, 1148
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 3 cut(s) 657, 1030, 1060
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 5 cut(s) 467, 651, 775, 881, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 887
HpyF3I CTNAG 2 cut(s) 75, 228
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 3 cut(s) 175, 779, 1146
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Kzo9I GATC 5 cut(s) 159, 204, 618, 872, 932
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 109, 785
Lsp1109I GCAGC 3 cut(s) 469, 865, 962
LweI GCATC 2 cut(s) 933, 1057
MaeI CTAG 4 cut(s) 611, 825, 867, 1049
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 3 cut(s) 217, 315, 1156
MalI GATC 5 cut(s) 161, 206, 620, 874, 934
MboI GATC 5 cut(s) 159, 204, 618, 872, 932
MboII GAAGA 5 cut(s) 293, 489, 557, 855, 1004
MfeI CAATTG 2 cut(s) 342, 624
MflI RGATCY 4 cut(s) 159, 618, 872, 932
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 2 cut(s) 128, 1098
MluI ACGCGT 1 cut(s) 714
MluNI TGGCCA 2 cut(s) 128, 1098
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 3 cut(s) 686, 760, 1021
Mox20I TGGCCA 2 cut(s) 128, 1098
Mph1103I ATGCAT 1 cut(s) 777
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 2 cut(s) 330, 549
MscI TGGCCA 2 cut(s) 128, 1098
MseI TTAA 5 cut(s) 377, 513, 531, 974, 1073
MslI CAYNNNNRTG 1 cut(s) 1005
Msp20I TGGCCA 2 cut(s) 128, 1098
MspI CCGG 2 cut(s) 41, 65
MspR9I CCNGG 1 cut(s) 142
MunI CAATTG 2 cut(s) 342, 624
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 2 cut(s) 157, 716
MwoI GCNNNNNNNGC 1 cut(s) 887
NaeI GCCGGC 1 cut(s) 42
NdeII GATC 5 cut(s) 159, 204, 618, 872, 932
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 175, 779, 1146
NlaIV GGNNCC 3 cut(s) 169, 620, 748
NmeAIII GCCGAG 2 cut(s) 74, 1016
NmuCI GTSAC 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 777
PaeR7I CTCGAG 1 cut(s) 233
PciSI GCTCTTC 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 330, 549
PfeI GAWTC 3 cut(s) 152, 239, 569
PflMI CCANNNNNTGG 3 cut(s) 214, 668, 986
PkrI GCNGC 4 cut(s) 484, 880, 952, 1087
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
PshBI ATTAAT 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 140
PspFI CCCAGC 1 cut(s) 842
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 169, 620, 748
PspPI GGNCC 1 cut(s) 168
PspXI VCTCGAGB 1 cut(s) 233
PstI CTGCAG 3 cut(s) 653, 883, 952
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 4 cut(s) 159, 618, 872, 932
RsaI GTAC 4 cut(s) 182, 706, 793, 996
RsaNI GTAC 4 cut(s) 181, 705, 792, 995
RseI CAYNNNNRTG 1 cut(s) 1005
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 5 cut(s) 377, 513, 531, 974, 1073
SatI GCNGC 4 cut(s) 483, 879, 951, 1086
Sau3AI GATC 5 cut(s) 159, 204, 618, 872, 932
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 2 cut(s) 933, 1057
SfcI CTRYAG 3 cut(s) 649, 879, 948
Sfr274I CTCGAG 1 cut(s) 233
SinI GGWCC 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 1005
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SsiI CCGC 5 cut(s) 157, 580, 809, 940, 1085
SspMI CTAG 4 cut(s) 611, 825, 867, 1049
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 3 cut(s) 657, 1030, 1060
TaiI ACGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 234
TatI WGTACW 2 cut(s) 180, 704
TauI GCSGC 1 cut(s) 1088
TfiI GAWTC 3 cut(s) 152, 239, 569
Tru1I TTAA 5 cut(s) 377, 513, 531, 974, 1073
Tru9I TTAA 5 cut(s) 377, 513, 531, 974, 1073
TscAI CASTG 3 cut(s) 547, 888, 1063
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 3 cut(s) 482, 878, 950
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 3 cut(s) 600, 654, 792
TspGWI ACGGA 2 cut(s) 389, 1117
TspRI CASTG 3 cut(s) 547, 888, 1063
Van91I CCANNNNNTGG 3 cut(s) 214, 668, 986
VpaK11BI GGWCC 1 cut(s) 168
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 2 cut(s) 692, 1101
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 2 cut(s) 330, 549
XspI CTAG 4 cut(s) 611, 825, 867, 1049
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.