RLG00000019510

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021525
Physical Location & Seq
Forward (+)
52943521 .. 52945787
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019510

Sequence Viewer

Length: 738 bp
ATGGATCTTTCGGTGTGTTGGACGGTAGCGGTGTTAGACTGCAGCGGTGCTGGTTTGGTGACTTGGTCATGGCAGTCTTCTAGCAGAGGTGAAGACTGGTCGTGGTATTCTACTCTTGCAGGCAGCAATGATCATGAGTGCTATGACATGTTCTGGTCTAAGTCGACTTGTTTTGGAGGCTGGAGAGGCGGCTCTAGTTCCGAAAGAAGGTTGGGTGTTGGTCATGGCGTTCGAGGTGGCGGAGCGGTGGCGGTGGCGGCGGAGGCGAACAAGTTGGCCGTAATTCTTCTAGGGTTTGTGTTGAATGGTTGTCTTGGGCCTGGATCTGGGCTTGGTTGGGAGTCCTTCTTTAGGGTGGTCTTGGACAGCGAGACGAGGGGGAATTGGGCAGCGAGAGCGATGGCAACATCATCCCAATCTTTCCGATCCAGATACATAATCTGCTTCAATCCTCCATTGAATGAGGACGAGTGGGGAGGAATTGATGAGGTCGTGAAGATCGGGAATGGAAGTCCGGCCAAAGCTGAGGCTGAGAGCATGGAGGAGGTGGACTCTAAGCTTACTGAGCTGAAGAGGTGCTTGGTAGATTTGTTTTATGGGACTGAATTGGGATTTCAGGCTGGGTCGGAGGTGAGAGCAGAAGTGGTGGAGTTGGTTAACGAATTGAAGGTGGCGAATCCCACTTCGGTGCCAACGAAGAACCCGGGGCTACTTGATGGCAACTGGGTTTTACTGTAA

Protein Analysis

246

Amino Acids

26.4

Weight (kDa)

4.76

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 189 - 245 6.9e-15 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021525)

Species Orthologous Gene IDs
rosa_laevigata RLG00000007934 RLG00000019510
rosa_multiflora Rmu_sc0003322.1_g000005 Rmu_sc0007667.1_g000001
rosa_samantha Rh5DG400000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 688
AccBSI CCGCTC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 164
AciI CCGC 8 cut(s) 29, 45, 189, 240, 245, 251, 257, 260
AclWI GGATC 3 cut(s) 12, 331, 420
AcoI YGGCCR 2 cut(s) 276, 516
AcuI CTGAAG 1 cut(s) 590
AfiI CCNNNNNNNGG 3 cut(s) 207, 326, 351
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 4 cut(s) 304, 448, 460, 667
AjnI CCWGG 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AleI CACNNNNGTG 1 cut(s) 686
AluBI AGCT 3 cut(s) 524, 559, 568
AluI AGCT 3 cut(s) 524, 559, 568
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 3 cut(s) 12, 331, 420
Ama87I CYCGRG 1 cut(s) 703
AoxI GGCC 3 cut(s) 276, 317, 516
ApeKI GCWGC 3 cut(s) 42, 123, 389
AspS9I GGNCC 1 cut(s) 317
AsuC2I CCSGG 2 cut(s) 704, 705
AsuHPI GGTGA 3 cut(s) 70, 101, 643
AvaI CYCGRG 1 cut(s) 703
BanI GGYRCC 1 cut(s) 688
BbsI GAAGAC 2 cut(s) 69, 99
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 54, 135, 401
BccI CCATC 2 cut(s) 394, 710
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 321
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 2 cut(s) 704, 705
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 3 cut(s) 81, 195, 290
BfmI CTRYAG 1 cut(s) 40
BisI GCNGC 5 cut(s) 43, 124, 190, 258, 390
BlsI GCNGC 5 cut(s) 44, 125, 191, 259, 391
Bme1390I CCNGG 3 cut(s) 321, 704, 705
BmeT110I CYCGRG 1 cut(s) 703
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 3 cut(s) 321, 704, 705
BmrI ACTGGG 1 cut(s) 733
BmuI ACTGGG 1 cut(s) 733
BpiI GAAGAC 2 cut(s) 69, 99
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 1 cut(s) 202
Bpu10I CCTNAGC 1 cut(s) 525
BpuMI CCSGG 2 cut(s) 704, 705
BsaJI CCNNGG 2 cut(s) 703, 704
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 326, 351
Bse1I ACTGG 2 cut(s) 101, 728
Bse3DI GCAATG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 703, 704
BseGI GGATG 1 cut(s) 410
BseLI CCNNNNNNNGG 3 cut(s) 207, 326, 351
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 3 cut(s) 516, 522, 555
BseNI ACTGG 2 cut(s) 101, 728
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 3 cut(s) 54, 135, 401
BseYI CCCAGC 1 cut(s) 620
BshFI GGCC 3 cut(s) 278, 319, 518
BshNI GGYRCC 1 cut(s) 688
BsiHKCI CYCGRG 1 cut(s) 703
BsiSI CCGG 2 cut(s) 515, 704
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 3 cut(s) 207, 326, 351
BsmAI GTCTC 1 cut(s) 365
BsmBI CGTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 3 cut(s) 278, 319, 518
BsoBI CYCGRG 1 cut(s) 703
Bsp143I GATC 5 cut(s) 4, 130, 323, 425, 498
BspACI CCGC 8 cut(s) 29, 45, 189, 240, 245, 251, 257, 260
BspANI GGCC 3 cut(s) 278, 319, 518
BspCNI CTCAG 3 cut(s) 517, 523, 556
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 1 cut(s) 690
BspMAI CTGCAG 1 cut(s) 44
BspPI GGATC 3 cut(s) 12, 331, 420
BspT107I GGYRCC 1 cut(s) 688
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 133
BsrI ACTGG 2 cut(s) 101, 728
BssECI CCNNGG 2 cut(s) 703, 704
BssMI GATC 5 cut(s) 4, 130, 323, 425, 498
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 25, 735
Bst6I CTCTTC 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 5 cut(s) 159, 525, 531, 555, 564
BstENI CCTNNNNNAGG 1 cut(s) 349
BstF5I GGATG 1 cut(s) 410
BstKTI GATC 5 cut(s) 7, 133, 326, 428, 501
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 5 cut(s) 4, 130, 323, 425, 498
BstMWI GCNNNNNNNGC 5 cut(s) 186, 257, 263, 395, 565
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 151
BstSCI CCNGG 3 cut(s) 319, 702, 703
BstSFI CTRYAG 1 cut(s) 40
BstV1I GCAGC 3 cut(s) 54, 135, 401
BstV2I GAAGAC 2 cut(s) 69, 99
BstX2I RGATCY 2 cut(s) 4, 323
BstYI RGATCY 2 cut(s) 4, 323
BsuRI GGCC 3 cut(s) 278, 319, 518
BtgZI GCGATG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 410
Cac8I GCNNGC 1 cut(s) 121
CciI TCATGA 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 317
Cfr9I CCCGGG 1 cut(s) 703
CviAII CATG 5 cut(s) 69, 134, 148, 224, 538
DdeI CTNAG 5 cut(s) 159, 525, 531, 555, 564
DpnI GATC 5 cut(s) 6, 132, 325, 427, 500
DpnII GATC 5 cut(s) 4, 130, 323, 425, 498
EaeI YGGCCR 2 cut(s) 276, 516
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
EciI GGCGGA 2 cut(s) 255, 275
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 703
EcoNI CCTNNNNNAGG 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 319
Esp3I CGTCTC 1 cut(s) 365
FaeI CATG 5 cut(s) 72, 137, 151, 227, 541
FaiI YATR 8 cut(s) 70, 135, 144, 149, 225, 437, 539, 597
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 1 cut(s) 613
FatI CATG 5 cut(s) 68, 133, 147, 223, 537
FbaI TGATCA 1 cut(s) 130
FblI GTMKAC 1 cut(s) 164
Fnu4HI GCNGC 5 cut(s) 43, 124, 190, 258, 390
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 5 cut(s) 43, 124, 190, 258, 390
FspBI CTAG 3 cut(s) 81, 195, 290
GluI GCNGC 5 cut(s) 43, 124, 190, 258, 390
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 3 cut(s) 278, 319, 518
HapII CCGG 2 cut(s) 515, 704
Hin1II CATG 5 cut(s) 72, 137, 151, 227, 541
HincII GTYRAC 2 cut(s) 165, 658
HindII GTYRAC 2 cut(s) 165, 658
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 341, 551, 676
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 2 cut(s) 515, 704
HphI GGTGA 3 cut(s) 70, 101, 643
Hpy166II GTNNAC 3 cut(s) 165, 550, 658
Hpy188I TCNGA 3 cut(s) 202, 425, 628
Hpy188III TCNNGA 4 cut(s) 134, 429, 493, 502
Hpy8I GTNNAC 3 cut(s) 165, 550, 658
HpyAV CCTTC 3 cut(s) 201, 355, 661
HpyCH4III ACNGT 2 cut(s) 25, 735
HpyCH4V TGCA 2 cut(s) 42, 119
HpyF10VI GCNNNNNNNGC 5 cut(s) 186, 257, 263, 395, 565
HpyF3I CTNAG 5 cut(s) 159, 525, 531, 555, 564
Hsp92II CATG 5 cut(s) 72, 137, 151, 227, 541
Ksp22I TGATCA 1 cut(s) 130
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 5 cut(s) 4, 130, 323, 425, 498
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 3 cut(s) 54, 135, 401
MaeI CTAG 3 cut(s) 81, 195, 290
MaeIII GTNAC 1 cut(s) 58
MalI GATC 5 cut(s) 6, 132, 325, 427, 500
MbiI CCGCTC 1 cut(s) 245
MboI GATC 5 cut(s) 4, 130, 323, 425, 498
MboII GAAGA 6 cut(s) 69, 104, 278, 508, 583, 709
MflI RGATCY 2 cut(s) 4, 323
MluCI AATT 5 cut(s) 282, 382, 480, 605, 662
MlyI GAGTC 2 cut(s) 350, 545
MmeI TCCRAC 1 cut(s) 606
MseI TTAA 1 cut(s) 657
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 2 cut(s) 515, 704
MspR9I CCNGG 3 cut(s) 321, 704, 705
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 5 cut(s) 186, 257, 263, 395, 565
NciI CCSGG 2 cut(s) 704, 705
NdeII GATC 5 cut(s) 4, 130, 323, 425, 498
NlaIII CATG 5 cut(s) 72, 137, 151, 227, 541
NlaIV GGNNCC 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 1 cut(s) 151
OliI CACNNNNGTG 1 cut(s) 686
PagI TCATGA 1 cut(s) 133
PciI ACATGT 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 692
PfeI GAWTC 1 cut(s) 676
PflFI GACNNNGTC 1 cut(s) 64
PkrI GCNGC 5 cut(s) 44, 125, 191, 259, 391
PleI GAGTC 2 cut(s) 349, 545
PpsI GAGTC 2 cut(s) 349, 545
PscI ACATGT 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 690
PspPI GGNCC 1 cut(s) 317
PstI CTGCAG 1 cut(s) 44
PsuI RGATCY 2 cut(s) 4, 323
PsyI GACNNNGTC 1 cut(s) 64
RseI CAYNNNNRTG 1 cut(s) 686
SalI GTCGAC 1 cut(s) 163
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 5 cut(s) 43, 124, 190, 258, 390
Sau3AI GATC 5 cut(s) 4, 130, 323, 425, 498
Sau96I GGNCC 1 cut(s) 317
SchI GAGTC 2 cut(s) 350, 545
ScrFI CCNGG 3 cut(s) 321, 704, 705
SfcI CTRYAG 1 cut(s) 40
SmaI CCCGGG 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 686
Sse9I AATT 5 cut(s) 282, 382, 480, 605, 662
SsiI CCGC 8 cut(s) 29, 45, 189, 240, 245, 251, 257, 260
SspMI CTAG 3 cut(s) 81, 195, 290
StyD4I CCNGG 3 cut(s) 319, 702, 703
TaaI ACNGT 2 cut(s) 25, 735
TaqI TCGA 2 cut(s) 164, 232
TasI AATT 5 cut(s) 282, 382, 480, 605, 662
TauI GCSGC 2 cut(s) 192, 260
TfiI GAWTC 1 cut(s) 676
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 3 cut(s) 42, 123, 389
Tsp45I GTSAC 1 cut(s) 58
TspMI CCCGGG 1 cut(s) 703
Tth111I GACNNNGTC 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 349
XceI RCATGY 1 cut(s) 151
XmaI CCCGGG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 164
XspI CTAG 3 cut(s) 81, 195, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.