Rh5DG400000

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0021525
Physical Location & Seq
Reverse (-)
63020336 .. 63020581
246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG400000.1

Sequence Viewer

Length: 246 bp
ATGAAGATTGGGAATGGAAGTCCGGCCAAAGCTGAGGCTGAGAGCACGGAGGAGGTGGACTCTAAGCTTACTGAGCTGAAGAGGTGCTTGGTAGATTTGTTTTATGGGACTGAATTGGGATTTCGGGCCGGGTCAGAGGTGAGAGCTGAAGTGGTGGAGTTGGTTAACGAATTAAAGGTGGCGAATCCCACTCCGGCGCCGATGAAGAACCCGGGGCTACTTGATGGCAACTGGGTTTTACTGTAA

Protein Analysis

81

Amino Acids

8.76

Weight (kDa)

4.73

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 25 - 81 1.3e-16 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021525)

Species Orthologous Gene IDs
rosa_laevigata RLG00000007934 RLG00000019510
rosa_multiflora Rmu_sc0003322.1_g000005 Rmu_sc0007667.1_g000001
rosa_samantha Rh5DG400000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 2 cut(s) 98, 168
AcyI GRCGYC 1 cut(s) 197
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 4 cut(s) 32, 67, 76, 146
AluI AGCT 4 cut(s) 32, 67, 76, 146
Alw21I GWGCWC 1 cut(s) 47
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 2 cut(s) 24, 126
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 3 cut(s) 130, 212, 213
AsuHPI GGTGA 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 211
BanI GGYRCC 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 33
BccI CCATC 1 cut(s) 218
BcnI CCSGG 3 cut(s) 130, 212, 213
BfoI RGCGCY 1 cut(s) 200
Bme1390I CCNGG 3 cut(s) 130, 212, 213
BmeT110I CYCGRG 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 3 cut(s) 130, 212, 213
BmrI ACTGGG 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 44, 76
Bpu10I CCTNAGC 1 cut(s) 33
BpuMI CCSGG 3 cut(s) 130, 212, 213
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 211, 212
Bse1I ACTGG 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 211, 212
BseMII CTCAG 3 cut(s) 24, 30, 63
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 1 cut(s) 65
BshFI GGCC 2 cut(s) 26, 128
BshNI GGYRCC 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 4 cut(s) 23, 129, 194, 212
BslFI GGGAC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 2 cut(s) 26, 128
BsoBI CYCGRG 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 47
BspANI GGCC 2 cut(s) 26, 128
BspCNI CTCAG 3 cut(s) 25, 31, 64
BspLI GGNNCC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 196
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 211, 212
BssNI GRCGYC 1 cut(s) 197
Bst4CI ACNGT 1 cut(s) 243
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 1 cut(s) 197
BstDEI CTNAG 4 cut(s) 33, 39, 63, 72
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 1 cut(s) 199
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 128, 210, 211
BsuRI GGCC 2 cut(s) 26, 128
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 126
Cfr9I CCCGGG 1 cut(s) 211
CviJI RGCY 8 cut(s) 26, 32, 38, 67, 76, 128, 146, 217
CviKI_1 RGCY 8 cut(s) 26, 32, 38, 67, 76, 128, 146, 217
DdeI CTNAG 4 cut(s) 33, 39, 63, 72
DinI GGCGCC 1 cut(s) 198
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco57I CTGAAG 2 cut(s) 98, 168
Eco88I CYCGRG 1 cut(s) 211
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
FaiI YATR 1 cut(s) 105
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 1 cut(s) 121
GlaI GCGC 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 2 cut(s) 26, 128
HapII CCGG 4 cut(s) 23, 129, 194, 212
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 197
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 2 cut(s) 59, 184
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 4 cut(s) 23, 129, 194, 212
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 2 cut(s) 58, 166
Hpy188I TCNGA 1 cut(s) 136
Hpy8I GTNNAC 2 cut(s) 58, 166
HpyCH4III ACNGT 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 4 cut(s) 33, 39, 63, 72
Hsp92I GRCGYC 1 cut(s) 197
HspAI GCGC 1 cut(s) 197
KasI GGCGCC 1 cut(s) 196
KspAI GTTAAC 1 cut(s) 166
LpnPI CCDG 5 cut(s) 36, 142, 207, 217, 225
MboII GAAGA 3 cut(s) 16, 91, 217
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 2 cut(s) 113, 170
Mly113I GGCGCC 1 cut(s) 197
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 5 cut(s) 28, 43, 46, 75, 130
MseI TTAA 2 cut(s) 165, 173
MspI CCGG 4 cut(s) 23, 129, 194, 212
MspR9I CCNGG 3 cut(s) 130, 212, 213
MwoI GCNNNNNNNGC 1 cut(s) 73
NarI GGCGCC 1 cut(s) 197
NciI CCSGG 3 cut(s) 130, 212, 213
NlaIV GGNNCC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 184
PleI GAGTC 1 cut(s) 53
PluTI GGCGCC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 126
SaqAI TTAA 2 cut(s) 165, 173
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 3 cut(s) 130, 212, 213
SduI GDGCHC 1 cut(s) 47
SetI ASST 8 cut(s) 34, 57, 69, 78, 86, 141, 148, 180
SfoI GGCGCC 1 cut(s) 198
SmaI CCCGGG 1 cut(s) 213
Sse9I AATT 2 cut(s) 113, 170
SspDI GGCGCC 1 cut(s) 196
StyD4I CCNGG 3 cut(s) 128, 210, 211
TaaI ACNGT 1 cut(s) 243
TasI AATT 2 cut(s) 113, 170
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 2 cut(s) 165, 173
Tru9I TTAA 2 cut(s) 165, 173
TspDTI ATGAA 2 cut(s) 17, 218
TspGWI ACGGA 1 cut(s) 62
TspMI CCCGGG 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.