RLG00000019629

Ninja-family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020613
Physical Location & Seq
Forward (+)
54548031 .. 54548767
737 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019629

Sequence Viewer

Length: 492 bp
ATGGAGGCAACCACCACCGCCGAGACTTCGGCTTCCTACTCTTCCTCATCCTCGTCTTATCAACCATCTCCGGCGGATCTGCGAATCGGATCCGGTTGGTTAAGAATCAGATCCCCAGCTGCTACGACATGTCGTCGGAAGCGCAGTGCTCCCAGAACCCCAAGTGCCGGTGGTGCCGCAGCGAGTCCCTGGACGATATGTGCTTCCCCAAGTCCTAATCTATGTGATTGCCCCCTCAGGAGGGAAATGAGGAGGAGGAGGAGCTGGAGGGAAGAGAACCACTACTCGGCGGCTCCGGCAGCGGCGAAGGCGGCACCGTACTCGGGACTGATGAGGGCGCCATCGGTTCTGAAGGAGACGGAGGATGAGTGGAGGAAGAGGAAGGAGTTGGAGATGCTATGGCGGTTAACGGCGAAGCAGAGGAGGTCGGAGAAGCATAGGAACAGCAGCAGCAGCAAGGAGGAAGAGCTGGCATTAACTAGTTCTTCATGA

Protein Analysis

164

Amino Acids

18.12

Weight (kDa)

10.14

Isoelectric Point (pI)

104.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NLS_NINJA_AFP PF16136 110 - 145 1.8e-10 Putative nuclear localisation signal in Ninja/AFP proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020613)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003618 RLG00000019629 RLG00000036609
rosa_multiflora Rmu_sc0002677.1_g000021
rosa_rugosa Rorug05G0349200 Rorug06G0268500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 173, 313, 337
AciI CCGC 7 cut(s) 18, 74, 177, 290, 302, 311, 403
AclWI GGATC 4 cut(s) 84, 84, 97, 105
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 338
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 5 cut(s) 167, 240, 241, 286, 323
AflIII ACRYGT 1 cut(s) 128
AhdI GACNNNNNGTC 1 cut(s) 132
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 3 cut(s) 119, 264, 469
AluI AGCT 3 cut(s) 119, 264, 469
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 2 cut(s) 17, 350
AlwI GGATC 4 cut(s) 84, 84, 97, 105
Ama87I CYCGRG 1 cut(s) 322
ApeKI GCWGC 6 cut(s) 119, 179, 299, 447, 450, 453
AspLEI GCGC 2 cut(s) 144, 340
AvaI CYCGRG 1 cut(s) 322
AxyI CCTNAGG 1 cut(s) 236
BamHI GGATCC 1 cut(s) 89
BanI GGYRCC 3 cut(s) 173, 313, 337
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 6 cut(s) 106, 191, 311, 459, 462, 465
BccI CCATC 2 cut(s) 73, 349
BceAI ACGGC 1 cut(s) 426
BcgI CGANNNNNNTGC 2 cut(s) 303, 337
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 17, 350
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 190
BmeRI GACNNNNNGTC 1 cut(s) 132
BmeT110I CYCGRG 1 cut(s) 322
BmiI GGNNCC 5 cut(s) 91, 175, 294, 315, 339
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 188
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 240, 241, 286, 323
Bse118I RCCGGY 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 2 cut(s) 47, 370
BseLI CCNNNNNNNGG 5 cut(s) 167, 240, 241, 286, 323
BseMII CTCAG 1 cut(s) 250
BseRI GAGGAG 5 cut(s) 265, 268, 271, 274, 436
BseXI GCAGC 6 cut(s) 106, 191, 311, 459, 462, 465
BseYI CCCAGC 1 cut(s) 115
BshNI GGYRCC 3 cut(s) 173, 313, 337
BsiHKAI GWGCWC 1 cut(s) 151
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 4 cut(s) 71, 93, 168, 296
BslFI GGGAC 2 cut(s) 171, 339
BslI CCNNNNNNNGG 5 cut(s) 167, 240, 241, 286, 323
BsmAI GTCTC 2 cut(s) 17, 350
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 2 cut(s) 171, 339
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 3 cut(s) 76, 89, 110
BspACI CCGC 7 cut(s) 18, 74, 177, 290, 302, 311, 403
BspCNI CTCAG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 488
BspLI GGNNCC 5 cut(s) 91, 175, 294, 315, 339
BspPI GGATC 4 cut(s) 84, 84, 97, 105
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 3 cut(s) 173, 313, 337
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 3 cut(s) 76, 89, 110
BssNI GRCGYC 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 4 cut(s) 46, 267, 371, 459
BstACI GRCGYC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 47, 370
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 2 cut(s) 144, 340
BstKTI GATC 3 cut(s) 79, 92, 113
BstMAI GTCTC 2 cut(s) 17, 350
BstMBI GATC 3 cut(s) 76, 89, 110
BstMWI GCNNNNNNNGC 6 cut(s) 173, 296, 299, 308, 311, 453
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 188
BstV1I GCAGC 6 cut(s) 106, 191, 311, 459, 462, 465
BstX2I RGATCY 3 cut(s) 76, 89, 110
BstYI RGATCY 3 cut(s) 76, 89, 110
Bsu36I CCTNAGG 1 cut(s) 236
BtsCI GGATG 2 cut(s) 47, 370
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 471
CciI TCATGA 1 cut(s) 488
CfoI GCGC 2 cut(s) 144, 340
Cfr10I RCCGGY 1 cut(s) 167
Csp6I GTAC 1 cut(s) 319
CviAII CATG 2 cut(s) 129, 489
CviJI RGCY 5 cut(s) 32, 119, 264, 293, 469
CviKI_1 RGCY 5 cut(s) 32, 119, 264, 293, 469
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 236
DinI GGCGCC 1 cut(s) 339
DpnI GATC 3 cut(s) 78, 91, 112
DpnII GATC 3 cut(s) 76, 89, 110
DriI GACNNNNNGTC 1 cut(s) 132
Eam1104I CTCTTC 4 cut(s) 46, 267, 371, 459
Eam1105I GACNNNNNGTC 1 cut(s) 132
EarI CTCTTC 4 cut(s) 46, 267, 371, 459
EciI GGCGGA 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 371
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 188
EgeI GGCGCC 1 cut(s) 339
EheI GGCGCC 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 2 cut(s) 132, 492
FaiI YATR 6 cut(s) 130, 199, 223, 400, 438, 490
FaqI GGGAC 2 cut(s) 171, 339
FatI CATG 2 cut(s) 128, 488
FokI GGATG 2 cut(s) 34, 377
FspBI CTAG 1 cut(s) 480
GlaI GCGC 2 cut(s) 143, 339
GsaI CCCAGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 286
HaeII RGCGCY 1 cut(s) 341
HapII CCGG 4 cut(s) 71, 93, 168, 296
HhaI GCGC 2 cut(s) 144, 340
Hin1I GRCGYC 1 cut(s) 338
Hin1II CATG 2 cut(s) 132, 492
Hin6I GCGC 2 cut(s) 142, 338
HinP1I GCGC 2 cut(s) 142, 338
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 3 cut(s) 84, 105, 184
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 4 cut(s) 71, 93, 168, 296
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 5 cut(s) 89, 110, 138, 351, 430
Hpy188III TCNNGA 3 cut(s) 238, 324, 489
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 3 cut(s) 301, 346, 376
HpyCH4III ACNGT 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 296, 299, 308, 311, 453
HpyF3I CTNAG 1 cut(s) 236
Hsp92I GRCGYC 1 cut(s) 338
Hsp92II CATG 2 cut(s) 132, 492
HspAI GCGC 2 cut(s) 142, 338
KasI GGCGCC 1 cut(s) 337
KspAI GTTAAC 1 cut(s) 408
Kzo9I GATC 3 cut(s) 76, 89, 110
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 3 cut(s) 154, 261, 298
Lsp1109I GCAGC 6 cut(s) 106, 191, 311, 459, 462, 465
LweI GCATC 1 cut(s) 384
MaeI CTAG 1 cut(s) 480
MalI GATC 3 cut(s) 78, 91, 112
MboI GATC 3 cut(s) 76, 89, 110
MboII GAAGA 5 cut(s) 33, 284, 388, 476, 477
MflI RGATCY 3 cut(s) 76, 89, 110
MhlI GDGCHC 1 cut(s) 151
Mly113I GGCGCC 1 cut(s) 338
MlyI GAGTC 1 cut(s) 193
MmeI TCCRAC 3 cut(s) 116, 369, 408
MseI TTAA 3 cut(s) 101, 407, 476
MspA1I CMGCKG 2 cut(s) 119, 302
MspI CCGG 4 cut(s) 71, 93, 168, 296
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 6 cut(s) 173, 296, 299, 308, 311, 453
NarI GGCGCC 1 cut(s) 338
NdeII GATC 3 cut(s) 76, 89, 110
NlaIII CATG 2 cut(s) 132, 492
NlaIV GGNNCC 5 cut(s) 91, 175, 294, 315, 339
NmeAIII GCCGAG 2 cut(s) 46, 266
NspI RCATGY 1 cut(s) 132
PagI TCATGA 1 cut(s) 488
PciI ACATGT 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 84, 105
PleI GAGTC 1 cut(s) 192
PluTI GGCGCC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 192
PscI ACATGT 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 115
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 5 cut(s) 91, 175, 294, 315, 339
PsuI RGATCY 3 cut(s) 76, 89, 110
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 101, 407, 476
Sau3AI GATC 3 cut(s) 76, 89, 110
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 151
SetI ASST 4 cut(s) 121, 266, 428, 471
SfaNI GCATC 1 cut(s) 384
SfoI GGCGCC 1 cut(s) 339
SpeI ACTAGT 1 cut(s) 479
SsiI CCGC 7 cut(s) 18, 74, 177, 290, 302, 311, 403
SspDI GGCGCC 1 cut(s) 337
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 318
TauI GCSGC 4 cut(s) 179, 293, 305, 314
TfiI GAWTC 2 cut(s) 84, 105
Tru1I TTAA 3 cut(s) 101, 407, 476
Tru9I TTAA 3 cut(s) 101, 407, 476
TscAI CASTG 1 cut(s) 151
TseI GCWGC 6 cut(s) 119, 179, 299, 447, 450, 453
TspDTI ATGAA 1 cut(s) 477
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 1 cut(s) 151
XceI RCATGY 1 cut(s) 132
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.