RLG00000019815

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020018
Physical Location & Seq
Forward (+)
57013738 .. 57015194
1457 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019815

Sequence Viewer

Length: 1116 bp
ATGAGTTCAGGAACAGGCAAGCGAATGTTTGAAGAGCACGACTCCTACCTCAATGATGGACCAACTATGTGCAAGAAAGCCAATACCTCTTCTGGGATTGATAATCAGTCCAGTGATGATGACTCGGATAGCAATGAAGAGTACAGTTGGCTGACTGAATTCTCGAACAAATTCGACAAGCTGAGTGCAGATTCTATTTTGGCAAAGTCTGGGTGGTTCTGTCCTGCATGCAAAAAAGGTGGCAGTCACGGTTGGTATAGAAAGTTCAATTCCCTGATATCCCTGCTTAATCATGCTAAGTCTATAAGCAGGACAAATAAGTTACACATGAAATTTACTCCAGAGTTGGAATCTATGGTGGAGAACAAGGACTTGAAACGCACTCCGAATCACCAACAGGTTGTTATGGATGATTGGGCAGGTATGGGAATTGCGGAGCTTTCCAAGTACTTTGGCGCTTATTCATATAAGAAAGGAAAACAATCGTATGGCTGTCAGGGACACCGTGGGATGAGCATTTTAATTTTTGATAAGTCGCTAAGGGGTTTCTTAGAGGCTAAGCGTCTGGAGAGGATCTTGCAAATCAAGGATCTGGAAGGGATGATTGGACCGAGCATGCTGTTGATGCTTCTGATCGTTCAAGAGGAGAAGGATCTTGTAACTATCATTTCCTTCCTGGCATTCCGAGAGGCGGCCAGAACTTCGGTTCTTAACTGGATCGATAACTATCAACCTACGGTTTTGGACAAAGTTTTTGTAACGTTTTCTCGGCCTAGAAACTTCCCTGTGCTTGTGGAAGAGTGTATCAGATTTGCTATTACGCGTGAAGTTAAGGCTCTAGGGCTTGATTTCTCTGATCCATCATGGGGTGATGATGTTTTACTTGGCAACAGTCCTGAAACTTCATTTGTCCTACCTCAAGTTGTTTATCAACATGGGGTTCTTGAATCTCTAACCCTCTTTTCTTGCAAGTTCAATGTGGCTGAGATAATTTTTCGTCTCTTGAAGCATCTTTCACTTGGTTGGATTGAACTGCCTCTACCTTCACTCACTGCGTTGTTACAGTTGTCAATTGCTCGAAAGCCTGAGTCTAAAGAACTGCTGGAACACCACTAG

Protein Analysis

372

Amino Acids

42.07

Weight (kDa)

6.57

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 135 - 194 1.9e-08 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020018)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019815
rosa_multiflora Rmu_ssc0000467.1_g000020
rosa_roxburghii Rroxscaffold_4G00322090
rosa_samantha Rh1AG088900 Rh1AG089000 Rh6BG159300 Rh6DG144700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 410
AccII CGCG 1 cut(s) 823
AciI CCGC 2 cut(s) 434, 692
AclI AACGTT 1 cut(s) 761
AclWI GGATC 5 cut(s) 581, 597, 660, 725, 851
AcoI YGGCCR 1 cut(s) 693
AcsI RAATTY 3 cut(s) 158, 170, 332
AfaI GTAC 2 cut(s) 143, 449
AfiI CCNNNNNNNGG 3 cut(s) 93, 691, 866
AflIII ACRYGT 1 cut(s) 821
AgsI TTSAA 8 cut(s) 32, 268, 376, 641, 947, 976, 1006, 1031
AjnI CCWGG 1 cut(s) 675
AjuI GAANNNNNNNTTGG 2 cut(s) 588, 620
AluBI AGCT 2 cut(s) 181, 439
AluI AGCT 2 cut(s) 181, 439
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 1004
AlwI GGATC 5 cut(s) 581, 597, 660, 725, 851
AoxI GGCC 2 cut(s) 693, 770
ApoI RAATTY 3 cut(s) 158, 170, 332
ArsI GACNNNNNNTTYG 2 cut(s) 737, 769
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 2 cut(s) 59, 608
AsuHPI GGTGA 2 cut(s) 383, 881
AvaII GGWCC 2 cut(s) 59, 608
Bbv12I GWGCWC 1 cut(s) 39
BccI CCATC 2 cut(s) 50, 868
BciT130I CCWGG 1 cut(s) 677
BcoDI GTCTC 1 cut(s) 1004
BfaI CTAG 3 cut(s) 774, 839, 1114
BfoI RGCGCY 1 cut(s) 459
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 1 cut(s) 693
BlpI GCTNAGC 1 cut(s) 558
BlsI GCNGC 1 cut(s) 694
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 1 cut(s) 677
Bme18I GGWCC 2 cut(s) 59, 608
BmgT120I GGNCC 2 cut(s) 59, 608
BmrFI CCNGG 1 cut(s) 677
BmsI GCATC 2 cut(s) 615, 1018
BplI GAGNNNNNCTC 2 cut(s) 26, 58
BpmI CTGGAG 2 cut(s) 324, 587
Bpu10I CCTNAGC 1 cut(s) 539
Bpu1102I GCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 720
BsaBI GATNNNNATC 1 cut(s) 726
BsaJI CCNNGG 1 cut(s) 505
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 691, 866
Bse1I ACTGG 2 cut(s) 111, 719
Bse3DI GCAATG 1 cut(s) 139
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 1 cut(s) 677
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 3 cut(s) 415, 516, 606
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 3 cut(s) 93, 691, 866
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 3 cut(s) 173, 975, 1077
BseNI ACTGG 2 cut(s) 111, 719
BseRI GAGGAG 1 cut(s) 659
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 823
BshFI GGCC 2 cut(s) 695, 772
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 1 cut(s) 39
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 3 cut(s) 93, 691, 866
BsmAI GTCTC 1 cut(s) 1004
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 1 cut(s) 513
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 2 cut(s) 695, 772
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 6 cut(s) 573, 589, 633, 652, 717, 856
Bsp1720I GCTNAGC 1 cut(s) 558
BspACI CCGC 2 cut(s) 434, 692
BspANI GGCC 2 cut(s) 695, 772
BspCNI CTCAG 3 cut(s) 174, 976, 1078
BspDI ATCGAT 1 cut(s) 720
BspFNI CGCG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 5 cut(s) 581, 597, 660, 725, 851
BspQI GCTCTTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 139
BsrI ACTGG 2 cut(s) 111, 719
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 6 cut(s) 573, 589, 633, 652, 717, 856
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 6 cut(s) 146, 251, 506, 739, 893, 1065
Bst6I CTCTTC 4 cut(s) 27, 94, 132, 792
BstC8I GCNNGC 3 cut(s) 20, 229, 617
BstDEI CTNAG 7 cut(s) 182, 297, 539, 550, 558, 984, 1086
BstDSI CCRYGG 1 cut(s) 505
BstF5I GGATG 3 cut(s) 415, 516, 606
BstFNI CGCG 1 cut(s) 823
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 6 cut(s) 576, 592, 636, 655, 720, 859
BstMAI GTCTC 1 cut(s) 1004
BstMBI GATC 6 cut(s) 573, 589, 633, 652, 717, 856
BstMWI GCNNNNNNNGC 1 cut(s) 625
BstNI CCWGG 1 cut(s) 677
BstNSI RCATGY 2 cut(s) 231, 619
BstSCI CCNGG 1 cut(s) 675
BstUI CGCG 1 cut(s) 823
BstX2I RGATCY 3 cut(s) 573, 589, 652
BstYI RGATCY 3 cut(s) 573, 589, 652
Bsu15I ATCGAT 1 cut(s) 720
BsuRI GGCC 2 cut(s) 695, 772
BsuTUI ATCGAT 1 cut(s) 720
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 3 cut(s) 415, 516, 606
BtsI GCAGTG 1 cut(s) 1050
BtsIMutI CAGTG 2 cut(s) 118, 1050
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 3 cut(s) 20, 229, 617
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 59, 608
ClaI ATCGAT 1 cut(s) 720
CseI GACGC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 142, 448
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 6 cut(s) 228, 293, 328, 616, 864, 935
CviQI GTAC 2 cut(s) 142, 448
DdeI CTNAG 7 cut(s) 182, 297, 539, 550, 558, 984, 1086
DpnI GATC 6 cut(s) 575, 591, 635, 654, 719, 858
DpnII GATC 6 cut(s) 573, 589, 633, 652, 717, 856
EaeI YGGCCR 1 cut(s) 693
Eam1104I CTCTTC 4 cut(s) 27, 94, 132, 792
EarI CTCTTC 4 cut(s) 27, 94, 132, 792
Eco32I GATATC 1 cut(s) 279
Eco47I GGWCC 2 cut(s) 59, 608
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 675
EcoRV GATATC 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 6 cut(s) 231, 296, 331, 619, 867, 938
FaqI GGGAC 1 cut(s) 513
FatI CATG 6 cut(s) 227, 292, 327, 615, 863, 934
Fnu4HI GCNGC 1 cut(s) 693
FokI GGATG 3 cut(s) 422, 523, 613
Fsp4HI GCNGC 1 cut(s) 693
FspBI CTAG 3 cut(s) 774, 839, 1114
GlaI GCGC 1 cut(s) 457
GluI GCNGC 1 cut(s) 693
GsuI CTGGAG 2 cut(s) 324, 587
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 2 cut(s) 695, 772
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 458
Hin1II CATG 6 cut(s) 231, 296, 331, 619, 867, 938
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 7 cut(s) 41, 122, 191, 350, 388, 947, 1088
HphI GGTGA 2 cut(s) 383, 881
Hpy188I TCNGA 6 cut(s) 127, 387, 633, 686, 809, 856
Hpy188III TCNNGA 9 cut(s) 9, 163, 341, 566, 593, 641, 896, 944, 1003
HpyAV CCTTC 4 cut(s) 590, 643, 682, 1053
HpyCH4III ACNGT 6 cut(s) 146, 251, 506, 739, 893, 1065
HpyCH4IV ACGT 1 cut(s) 761
HpyCH4V TGCA 6 cut(s) 72, 188, 227, 231, 580, 969
HpyF10VI GCNNNNNNNGC 1 cut(s) 625
HpyF3I CTNAG 7 cut(s) 182, 297, 539, 550, 558, 984, 1086
HpySE526I ACGT 1 cut(s) 761
Hsp92II CATG 6 cut(s) 231, 296, 331, 619, 867, 938
HspAI GCGC 1 cut(s) 456
Kzo9I GATC 6 cut(s) 573, 589, 633, 652, 717, 856
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 436
LweI GCATC 2 cut(s) 615, 1018
MaeI CTAG 3 cut(s) 774, 839, 1114
MaeII ACGT 1 cut(s) 761
MaeIII GTNAC 5 cut(s) 245, 321, 658, 757, 1059
MalI GATC 6 cut(s) 575, 591, 635, 654, 719, 858
MboI GATC 6 cut(s) 573, 589, 633, 652, 717, 856
MboII GAAGA 4 cut(s) 44, 81, 149, 809
MfeI CAATTG 1 cut(s) 1071
MflI RGATCY 3 cut(s) 573, 589, 652
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 8 cut(s) 158, 170, 268, 332, 429, 522, 990, 1071
MluI ACGCGT 1 cut(s) 821
MlyI GAGTC 3 cut(s) 35, 116, 1097
MmeI TCCRAC 2 cut(s) 327, 1004
MnlI CCTC 9 cut(s) 59, 97, 547, 564, 637, 682, 927, 968, 1047
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 4 cut(s) 288, 521, 711, 831
MspR9I CCNGG 1 cut(s) 677
MunI CAATTG 1 cut(s) 1071
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 1 cut(s) 677
MvnI CGCG 1 cut(s) 823
MwoI GCNNNNNNNGC 1 cut(s) 625
NdeII GATC 6 cut(s) 573, 589, 633, 652, 717, 856
NlaIII CATG 6 cut(s) 231, 296, 331, 619, 867, 938
NmeAIII GCCGAG 1 cut(s) 748
NmuCI GTSAC 1 cut(s) 245
NspI RCATGY 2 cut(s) 231, 619
PaeI GCATGC 2 cut(s) 231, 619
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 4 cut(s) 191, 350, 388, 947
PkrI GCNGC 1 cut(s) 694
PleI GAGTC 3 cut(s) 35, 116, 1096
PpsI GAGTC 3 cut(s) 35, 116, 1096
Psp1406I AACGTT 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspPI GGNCC 2 cut(s) 59, 608
PsrI GAACNNNNNNTAC 4 cut(s) 248, 280, 1023, 1055
PsuI RGATCY 3 cut(s) 573, 589, 652
RsaI GTAC 2 cut(s) 143, 449
RsaNI GTAC 2 cut(s) 142, 448
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 4 cut(s) 288, 521, 711, 831
SatI GCNGC 1 cut(s) 693
Sau3AI GATC 6 cut(s) 573, 589, 633, 652, 717, 856
Sau96I GGNCC 2 cut(s) 59, 608
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 3 cut(s) 35, 116, 1097
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 2 cut(s) 615, 1018
SinI GGWCC 2 cut(s) 59, 608
SmlI CTYRAG 1 cut(s) 918
SmoI CTYRAG 1 cut(s) 918
SphI GCATGC 2 cut(s) 231, 619
Sse9I AATT 8 cut(s) 158, 170, 268, 332, 429, 522, 990, 1071
SsiI CCGC 2 cut(s) 434, 692
SspMI CTAG 3 cut(s) 774, 839, 1114
StyD4I CCNGG 1 cut(s) 675
TaaI ACNGT 6 cut(s) 146, 251, 506, 739, 893, 1065
TaiI ACGT 1 cut(s) 764
TaqI TCGA 4 cut(s) 164, 174, 720, 1078
TaqII GACCGA 1 cut(s) 625
TasI AATT 8 cut(s) 158, 170, 268, 332, 429, 522, 990, 1071
TatI WGTACW 2 cut(s) 141, 447
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 4 cut(s) 191, 350, 388, 947
Tru1I TTAA 4 cut(s) 288, 521, 711, 831
Tru9I TTAA 4 cut(s) 288, 521, 711, 831
TscAI CASTG 2 cut(s) 118, 1057
TseFI GTSAC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 4 cut(s) 150, 344, 453, 894
TspRI CASTG 2 cut(s) 118, 1057
VpaK11BI GGWCC 2 cut(s) 59, 608
XapI RAATTY 3 cut(s) 158, 170, 332
XceI RCATGY 2 cut(s) 231, 619
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 3 cut(s) 774, 839, 1114
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.