Rh1AG089000

Protein SUPPRESSOR OF GENE SILENCING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0020018
Physical Location & Seq
Forward (+)
15277018 .. 15277362
345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG089000.1

Sequence Viewer

Length: 345 bp
ATGATCTTAGTCAGGAATACTAATGCAATGGATGACAGTAAAGGTATAGGAATTGCAGAGCTTTCCAAGTACTTTGGCGCTTATTCATATAAGAAAGGAAAACAATCGTATGGCCGTCAAGGACACCGTGGGATGAGCGTTTTAATTTTTGATAAGTCGCTAAGGGGTTTCTTAGAGGCAAAGCGTTTGGAGAAGGATCTTGCAGATCAAGGATCTAGAAGGGATGATTGGACCGAGCACGCTATTGATGCTTCTGGTAAGCGATTGCTTTATGGTTACATGCCAAATGAAGAAGATTTGATTACATTCAAATCGAAGGTGAAATATGAGATCAAGTCATTGTAG

Protein Analysis

114

Amino Acids

13.05

Weight (kDa)

9.4

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 2 - 100 2.1e-17 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020018)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019815
rosa_multiflora Rmu_ssc0000467.1_g000020
rosa_roxburghii Rroxscaffold_4G00322090
rosa_samantha Rh1AG088900 Rh1AG089000 Rh6BG159300 Rh6DG144700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 204, 220
AcoI YGGCCR 1 cut(s) 112
AfaI GTAC 1 cut(s) 71
AgsI TTSAA 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 211, 243
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
Alw21I GWGCWC 1 cut(s) 240
AlwI GGATC 2 cut(s) 204, 220
AoxI GGCC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 240
BceAI ACGGC 1 cut(s) 99
BfaI CTAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 81
BmcAI AGTACT 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 231
BmsI GCATC 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 33
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 3 cut(s) 37, 138, 229
BseMI GCAATG 1 cut(s) 33
BshFI GGCC 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 240
BsnI GGCC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 5 cut(s) 3, 196, 205, 212, 330
BspANI GGCC 1 cut(s) 114
BspPI GGATC 2 cut(s) 204, 220
BsrDI GCAATG 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 5 cut(s) 3, 196, 205, 212, 330
Bst4CI ACNGT 2 cut(s) 38, 128
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 3 cut(s) 7, 161, 172
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 3 cut(s) 37, 138, 229
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 5 cut(s) 6, 199, 208, 215, 333
BstMBI GATC 5 cut(s) 3, 196, 205, 212, 330
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 283
BstX2I RGATCY 2 cut(s) 196, 212
BstYI RGATCY 2 cut(s) 196, 212
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 3 cut(s) 37, 138, 229
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 70
CviAII CATG 1 cut(s) 280
CviJI RGCY 2 cut(s) 61, 114
CviKI_1 RGCY 2 cut(s) 61, 114
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 3 cut(s) 7, 161, 172
DpnI GATC 5 cut(s) 5, 198, 207, 214, 332
DpnII GATC 5 cut(s) 3, 196, 205, 212, 330
EaeI YGGCCR 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 231
FaeI CATG 1 cut(s) 283
FaiI YATR 7 cut(s) 47, 88, 90, 111, 273, 281, 327
FatI CATG 1 cut(s) 279
FokI GGATG 3 cut(s) 44, 145, 236
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 79
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 114
HhaI GCGC 1 cut(s) 80
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HphI GGTGA 1 cut(s) 331
Hpy188III TCNNGA 2 cut(s) 13, 216
HpyAV CCTTC 3 cut(s) 187, 213, 310
HpyCH4III ACNGT 2 cut(s) 38, 128
HpyCH4V TGCA 3 cut(s) 26, 56, 203
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 7, 161, 172
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 5 cut(s) 3, 196, 205, 212, 330
LpnPI CCDG 1 cut(s) 240
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 275
MalI GATC 5 cut(s) 5, 198, 207, 214, 332
MboI GATC 5 cut(s) 3, 196, 205, 212, 330
MboII GAAGA 2 cut(s) 302, 305
MflI RGATCY 2 cut(s) 196, 212
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 51, 144
MnlI CCTC 1 cut(s) 169
MseI TTAA 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 5 cut(s) 3, 196, 205, 212, 330
NlaIII CATG 1 cut(s) 283
NspI RCATGY 1 cut(s) 283
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 2 cut(s) 196, 212
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 143
Sau3AI GATC 5 cut(s) 3, 196, 205, 212, 330
Sau96I GGNCC 1 cut(s) 231
ScaI AGTACT 1 cut(s) 71
SduI GDGCHC 1 cut(s) 240
SetI ASST 3 cut(s) 46, 63, 321
SfaNI GCATC 1 cut(s) 238
SinI GGWCC 1 cut(s) 231
Sse9I AATT 2 cut(s) 51, 144
SspMI CTAG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 38, 128
TaqI TCGA 1 cut(s) 314
TaqII GACCGA 1 cut(s) 248
TasI AATT 2 cut(s) 51, 144
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 75, 303
VpaK11BI GGWCC 1 cut(s) 231
XbaI TCTAGA 1 cut(s) 215
XceI RCATGY 1 cut(s) 283
XspI CTAG 1 cut(s) 216
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.