Rh6BG159300

Protein SUPPRESSOR OF GENE SILENCING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0020018
Physical Location & Seq
Forward (+)
25868510 .. 25868911
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG159300.1

Sequence Viewer

Length: 402 bp
ATGATCTTAGTCAGGAATACTAATGCAATAGATGGCAGTAAAGGTATAGGAATTGCAGAGCTTTCCAAGTACTTTGGCGCTTATTCATATAAGAAAGGAAAACAATCGTATGGCCGTCAGGGACACCGTGGGATGATCATTTTAATTTTTGTTAAGTCGCTAAGGGGTTTCTTAGAGGCTAAGCGTCTGGAGAGGGATCTTGCAGATCAAGGATCTGGAACGAATGATTGGACCGAGCACGCTGTCGATGCTTCTGGTAAGCGATTGCTTTATGGTTACATGACAAATGAAGAAGATTTGATTACATTCAAATCGAAGGTAAAATATGAGATCAAGTCATTGCAGCAAATCGTTCAAGAGGAGAATGATCAATCGGAGGATTATGGTTCAGAGGTACAATGA

Protein Analysis

133

Amino Acids

15.06

Weight (kDa)

6.74

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 11 - 100 3.9e-16 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020018)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019815
rosa_multiflora Rmu_ssc0000467.1_g000020
rosa_roxburghii Rroxscaffold_4G00322090
rosa_samantha Rh1AG088900 Rh1AG089000 Rh6BG159300 Rh6DG144700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 204, 220
AcoI YGGCCR 1 cut(s) 112
AfaI GTAC 2 cut(s) 71, 396
AgsI TTSAA 2 cut(s) 310, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 211, 243
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
Alw21I GWGCWC 1 cut(s) 240
AlwI GGATC 2 cut(s) 204, 220
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 1 cut(s) 343
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 231
AvaII GGWCC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 355
BccI CCATC 1 cut(s) 26
BceAI ACGGC 1 cut(s) 99
BclI TGATCA 2 cut(s) 135, 367
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 345
BmcAI AGTACT 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 231
BmsI GCATC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 161
Bpu1102I GCTNAGC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 1 cut(s) 138
BseMI GCAATG 1 cut(s) 338
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 355
BshFI GGCC 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 240
BslFI GGGAC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 135
BsnI GGCC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
Bsp1720I GCTNAGC 1 cut(s) 180
BspANI GGCC 1 cut(s) 114
BspPI GGATC 2 cut(s) 204, 220
BsrDI GCAATG 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
Bst4CI ACNGT 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 4 cut(s) 7, 161, 172, 180
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 7 cut(s) 6, 138, 199, 208, 215, 333, 370
BstMBI GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 355
BstX2I RGATCY 2 cut(s) 196, 212
BstYI RGATCY 2 cut(s) 196, 212
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 70, 395
CviAII CATG 1 cut(s) 280
CviJI RGCY 3 cut(s) 61, 114, 179
CviKI_1 RGCY 3 cut(s) 61, 114, 179
CviQI GTAC 2 cut(s) 70, 395
DdeI CTNAG 4 cut(s) 7, 161, 172, 180
DpnI GATC 7 cut(s) 5, 137, 198, 207, 214, 332, 369
DpnII GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
EaeI YGGCCR 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 231
FaeI CATG 1 cut(s) 283
FaiI YATR 8 cut(s) 47, 88, 90, 111, 273, 281, 327, 384
FaqI GGGAC 1 cut(s) 135
FatI CATG 1 cut(s) 279
FbaI TGATCA 2 cut(s) 135, 367
Fnu4HI GCNGC 1 cut(s) 344
FokI GGATG 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 344
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 114
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 80
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
Hpy188I TCNGA 2 cut(s) 376, 391
Hpy188III TCNNGA 4 cut(s) 13, 188, 216, 356
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 4 cut(s) 26, 56, 203, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 4 cut(s) 7, 161, 172, 180
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 135, 367
Kzo9I GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
LpnPI CCDG 4 cut(s) 104, 173, 201, 240
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 1 cut(s) 238
MaeIII GTNAC 1 cut(s) 275
MalI GATC 7 cut(s) 5, 137, 198, 207, 214, 332, 369
MboI GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
MboII GAAGA 2 cut(s) 302, 305
MflI RGATCY 2 cut(s) 196, 212
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 51, 144
MnlI CCTC 5 cut(s) 169, 186, 352, 370, 385
MseI TTAA 2 cut(s) 143, 153
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
NlaIII CATG 1 cut(s) 283
PkrI GCNGC 1 cut(s) 345
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 2 cut(s) 196, 212
RsaI GTAC 2 cut(s) 71, 396
RsaNI GTAC 2 cut(s) 70, 395
SaqAI TTAA 2 cut(s) 143, 153
SatI GCNGC 1 cut(s) 344
Sau3AI GATC 7 cut(s) 3, 135, 196, 205, 212, 330, 367
Sau96I GGNCC 1 cut(s) 231
ScaI AGTACT 1 cut(s) 71
SduI GDGCHC 1 cut(s) 240
SetI ASST 4 cut(s) 46, 63, 321, 396
SfaNI GCATC 1 cut(s) 238
SinI GGWCC 1 cut(s) 231
Sse9I AATT 2 cut(s) 51, 144
TaaI ACNGT 1 cut(s) 128
TaqI TCGA 2 cut(s) 246, 314
TaqII GACCGA 1 cut(s) 248
TasI AATT 2 cut(s) 51, 144
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 2 cut(s) 143, 153
Tru9I TTAA 2 cut(s) 143, 153
TseI GCWGC 1 cut(s) 343
TspDTI ATGAA 2 cut(s) 75, 303
VpaK11BI GGWCC 1 cut(s) 231
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.