RLG00000019978

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018461
Physical Location & Seq
Reverse (-)
59495637 .. 59497481
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019978

Sequence Viewer

Length: 1239 bp
ATGGATAACAAGAAGTCCTCTTCAAGTGCTTTGATAGCATTACCTTCGCCAATCAAAGAAGATGAAATTGAATGGAAGAAGAGCTTACCATCTGGAGTGAAGTTTCGGCCCTCACATAAAGATTTATATCATTATCTCCATATGAAGGTTCATACTGGGCGCATTGATTTTGGTGGAGTTATTAGAAGCATCAATCTCTATATATATAAGCCCGAGGACCTAAGAGGTTTAGCATTTGATAATGATGATGGCCGCTTGTACTTCTTCACACCATTGCAAAAGAAGTTCACAAAGGGAAAAAAGACAAACAGAGTAACCGGACATGGTTTTTGGAAGGTCACACAGAAGTCTGTAAAGATTATGGACGATGACAACAAGCCTATTATGACCAAAACACCTCTGTGTTACTTTTACTTTTGGGGCAAAAAGGGAAGGAAAACTCAGTGGCTCATGGACCAGTACAAGCTCATCAACGAGGGCAGTAATCCTGTGATGTTGAAGAATTGGGTTCTTTGTGTTGTCCACTATAAGCCAAGAGGGAATAATGATACAAACAAAAGAAGCAGCACTGATTCAAGGTCCGAGACAGTGGGATCTTCTCCTCAAAACTCGATGATAGGTCCGTCTTCCGAATCCAATCCCCAAATTTCCTTTGGCAACAATAACTTCAATGTGGCTTCATCTTCTAACCCACAAGGTACTAGTTCATACCCAGAACCGGTGGCATTCCAGACTCCTAGTAACCCAAATAAGAGACAGCAGTGTTCTACTTCTTATGATGAGCCTTTGTGTATCACCTGCCCTGGTTATAACATTGCCTCTTATGCCCAATTTCCTAAACGTAGATCACCTAGTGCTAATGACACCCCCAACATTGATCAAAACCAAGCTAGTGACTCTTCTTTTGATCAAATGGTTTCTAACGTACTCAACCAGGATCCTGATGAATACACGTGTCTAGAGCCATTACTCAACCTAGATATAGGCGATTACGAGTGGAAAGACGATATTTTTAATTATCTTTTTCCATCTGAACTTAACCCTCAAAATAGTAACCCTAATAGCATGAACCCTACAACTACTACTACTAGTCATCCTACAGTCTCTGCCTCAGATCCACCTCCTCCCACCTTCAACTCTTCTGCCGATGAAAATGCCAGGATAGCATCTATGGCAAAAGTGGGTCAATCCAATGCCACAACAAGCTTATCGATCACTGAGGAAGGGAAGACAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

413

Amino Acids

46.23

Weight (kDa)

8.34

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 30 - 156 1.4e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018461)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g33050
rosa_laevigata RLG00000019978
rosa_multiflora Rmu_sc0004112.1_g000016
rosa_roxburghii Rroxscaffold_2G00102400
rosa_rugosa Rorug02G0374700
rosa_samantha Rh2AG427000 Rh2BG436300 Rh2CG413500 Rh2DG446200
rosa_wichuraiana Rw2G034980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 810
AarI CACCTGC 1 cut(s) 806
Acc36I ACCTGC 1 cut(s) 806
AciI CCGC 1 cut(s) 253
AclWI GGATC 4 cut(s) 601, 932, 945, 1109
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 1 cut(s) 645
AcvI CACGTG 1 cut(s) 954
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 4 cut(s) 260, 461, 700, 927
AfiI CCNNNNNNNGG 2 cut(s) 145, 718
AflIII ACRYGT 2 cut(s) 951, 953
AgeI ACCGGT 1 cut(s) 718
AgsI TTSAA 6 cut(s) 24, 71, 499, 576, 670, 1135
AhlI ACTAGT 2 cut(s) 701, 1088
AjnI CCWGG 3 cut(s) 802, 933, 1157
AleI CACNNNNGTG 1 cut(s) 400
AluBI AGCT 4 cut(s) 84, 466, 890, 1206
AluI AGCT 4 cut(s) 84, 466, 890, 1206
Alw26I GTCTC 3 cut(s) 578, 748, 1108
AlwI GGATC 4 cut(s) 601, 932, 945, 1109
AlwNI CAGNNNCTG 1 cut(s) 1106
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 107, 250
ApeKI GCWGC 1 cut(s) 564
ApoI RAATTY 1 cut(s) 645
ArsI GACNNNNNNTTYG 4 cut(s) 312, 344, 887, 919
AsiGI ACCGGT 1 cut(s) 718
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 5 cut(s) 108, 217, 454, 579, 620
AsuHPI GGTGA 2 cut(s) 787, 840
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 4 cut(s) 217, 454, 579, 620
BamHI GGATCC 1 cut(s) 937
BbrPI CACGTG 1 cut(s) 954
BbsI GAAGAC 2 cut(s) 618, 1235
BbvI GCAGC 1 cut(s) 576
BccI CCATC 3 cut(s) 97, 242, 1036
BcgI CGANNNNNNTGC 4 cut(s) 27, 61, 1136, 1170
BciT130I CCWGG 3 cut(s) 804, 935, 1159
BclI TGATCA 2 cut(s) 877, 907
BcoDI GTCTC 3 cut(s) 578, 748, 1108
BcuI ACTAGT 2 cut(s) 701, 1088
BfaI CTAG 7 cut(s) 702, 738, 852, 891, 959, 977, 1089
BfmI CTRYAG 1 cut(s) 1098
BfuAI ACCTGC 1 cut(s) 806
BisI GCNGC 2 cut(s) 253, 565
BlsI GCNGC 2 cut(s) 254, 566
Bme1390I CCNGG 3 cut(s) 804, 935, 1159
Bme18I GGWCC 4 cut(s) 217, 454, 579, 620
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 5 cut(s) 108, 217, 454, 579, 620
BmiI GGNNCC 1 cut(s) 939
BmrFI CCNGG 3 cut(s) 804, 935, 1159
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 198, 1175
BmuI ACTGGG 1 cut(s) 165
BpiI GAAGAC 2 cut(s) 618, 1235
BpmI CTGGAG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 1211
BsaAI YACGTR 1 cut(s) 954
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 213, 802
BsaWI WCCGGW 2 cut(s) 317, 718
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 718
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 2 cut(s) 160, 457
Bse3DI GCAATG 2 cut(s) 272, 813
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 3 cut(s) 804, 935, 1159
BseCI ATCGAT 1 cut(s) 1211
BseDI CCNNGG 2 cut(s) 213, 802
BseGI GGATG 1 cut(s) 1093
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 145, 718
BseMI GCAATG 2 cut(s) 272, 813
BseMII CTCAG 3 cut(s) 455, 1125, 1209
BseNI ACTGG 2 cut(s) 160, 457
BseRI GAGGAG 2 cut(s) 591, 1113
BseXI GCAGC 1 cut(s) 576
BshFI GGCC 2 cut(s) 109, 252
BshTI ACCGGT 1 cut(s) 718
BshVI ATCGAT 1 cut(s) 1211
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 318, 719
BslI CCNNNNNNNGG 2 cut(s) 145, 718
BsmAI GTCTC 3 cut(s) 578, 748, 1108
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 2 cut(s) 109, 252
BsoBI CYCGRG 1 cut(s) 212
Bsp143I GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
BspACI CCGC 1 cut(s) 253
BspANI GGCC 2 cut(s) 109, 252
BspCNI CTCAG 3 cut(s) 454, 1124, 1210
BspDI ATCGAT 1 cut(s) 1211
BspLI GGNNCC 1 cut(s) 939
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 4 cut(s) 601, 932, 945, 1109
BspQI GCTCTTC 1 cut(s) 74
BsrDI GCAATG 2 cut(s) 272, 813
BsrFI RCCGGY 1 cut(s) 718
BsrI ACTGG 2 cut(s) 160, 457
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 2 cut(s) 213, 802
BssMI GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
Bst2UI CCWGG 3 cut(s) 804, 935, 1159
Bst4CI ACNGT 2 cut(s) 589, 1102
Bst6I CTCTTC 4 cut(s) 25, 74, 904, 1144
BstBAI YACGTR 1 cut(s) 954
BstDEI CTNAG 4 cut(s) 221, 441, 1111, 1218
BstF5I GGATG 1 cut(s) 1093
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 7 cut(s) 596, 848, 880, 910, 940, 1117, 1215
BstMAI GTCTC 3 cut(s) 578, 748, 1108
BstMBI GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
BstMWI GCNNNNNNNGC 4 cut(s) 35, 824, 1163, 1172
BstNI CCWGG 3 cut(s) 804, 935, 1159
BstSCI CCNGG 3 cut(s) 802, 933, 1157
BstSFI CTRYAG 1 cut(s) 1098
BstV1I GCAGC 1 cut(s) 576
BstV2I GAAGAC 2 cut(s) 618, 1235
BstX2I RGATCY 3 cut(s) 593, 937, 1114
BstYI RGATCY 3 cut(s) 593, 937, 1114
Bsu15I ATCGAT 1 cut(s) 1211
BsuRI GGCC 2 cut(s) 109, 252
BsuTUI ATCGAT 1 cut(s) 1211
BtsCI GGATG 1 cut(s) 1093
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 5 cut(s) 449, 567, 594, 767, 1215
BveI ACCTGC 1 cut(s) 806
CaiI CAGNNNCTG 1 cut(s) 1106
CfoI GCGC 1 cut(s) 162
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 5 cut(s) 108, 217, 454, 579, 620
ClaI ATCGAT 1 cut(s) 1211
Csp6I GTAC 4 cut(s) 259, 460, 699, 926
CspAI ACCGGT 1 cut(s) 718
CviAII CATG 3 cut(s) 323, 451, 1066
CviQI GTAC 4 cut(s) 259, 460, 699, 926
DdeI CTNAG 4 cut(s) 221, 441, 1111, 1218
DpnI GATC 7 cut(s) 595, 847, 879, 909, 939, 1116, 1214
DpnII GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 4 cut(s) 25, 74, 904, 1144
EarI CTCTTC 4 cut(s) 25, 74, 904, 1144
Eco47I GGWCC 4 cut(s) 217, 454, 579, 620
Eco72I CACGTG 1 cut(s) 954
Eco88I CYCGRG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 3 cut(s) 802, 933, 1157
FaeI CATG 3 cut(s) 326, 454, 1069
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 3 cut(s) 322, 450, 1065
FauNDI CATATG 1 cut(s) 141
FbaI TGATCA 2 cut(s) 877, 907
Fnu4HI GCNGC 2 cut(s) 253, 565
FokI GGATG 1 cut(s) 1080
Fsp4HI GCNGC 2 cut(s) 253, 565
FspBI CTAG 7 cut(s) 702, 738, 852, 891, 959, 977, 1089
GlaI GCGC 1 cut(s) 161
GluI GCNGC 2 cut(s) 253, 565
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 2 cut(s) 109, 252
HapII CCGG 2 cut(s) 318, 719
HhaI GCGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 326, 454, 1069
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 1204
HinfI GANTC 4 cut(s) 572, 632, 733, 896
HpaII CCGG 2 cut(s) 318, 719
HphI GGTGA 2 cut(s) 787, 840
Hpy166II GTNNAC 2 cut(s) 288, 523
Hpy188I TCNGA 4 cut(s) 583, 631, 1033, 1114
Hpy188III TCNNGA 4 cut(s) 93, 730, 941, 959
Hpy8I GTNNAC 2 cut(s) 288, 523
HpyAV CCTTC 6 cut(s) 54, 139, 328, 426, 1141, 1217
HpyCH4III ACNGT 2 cut(s) 589, 1102
HpyCH4IV ACGT 3 cut(s) 841, 924, 953
HpyCH4V TGCA 1 cut(s) 277
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 824, 1163, 1172
HpyF3I CTNAG 4 cut(s) 221, 441, 1111, 1218
HpySE526I ACGT 3 cut(s) 841, 924, 953
Hsp92II CATG 3 cut(s) 326, 454, 1069
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 2 cut(s) 877, 907
Kzo9I GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
LguI GCTCTTC 1 cut(s) 74
Lsp1109I GCAGC 1 cut(s) 576
LweI GCATC 2 cut(s) 198, 1175
MaeI CTAG 7 cut(s) 702, 738, 852, 891, 959, 977, 1089
MaeII ACGT 3 cut(s) 841, 924, 953
MaeIII GTNAC 6 cut(s) 313, 337, 404, 740, 893, 1052
MalI GATC 7 cut(s) 595, 847, 879, 909, 939, 1116, 1214
MboI GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
MflI RGATCY 3 cut(s) 593, 937, 1114
MluCI AATT 5 cut(s) 66, 502, 645, 830, 1015
MlyI GAGTC 2 cut(s) 727, 890
MseI TTAA 2 cut(s) 1014, 1038
MslI CAYNNNNRTG 1 cut(s) 400
MspI CCGG 2 cut(s) 318, 719
MspR9I CCNGG 3 cut(s) 804, 935, 1159
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 3 cut(s) 804, 935, 1159
MwoI GCNNNNNNNGC 4 cut(s) 35, 824, 1163, 1172
NdeI CATATG 1 cut(s) 141
NdeII GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
NlaIII CATG 3 cut(s) 326, 454, 1069
NlaIV GGNNCC 1 cut(s) 939
NmuCI GTSAC 2 cut(s) 337, 893
OliI CACNNNNGTG 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 806
PciSI GCTCTTC 1 cut(s) 74
PctI GAATGC 1 cut(s) 725
PfeI GAWTC 2 cut(s) 572, 632
PinAI ACCGGT 1 cut(s) 718
PkrI GCNGC 2 cut(s) 254, 566
PleI GAGTC 2 cut(s) 727, 890
PmaCI CACGTG 1 cut(s) 954
PmlI CACGTG 1 cut(s) 954
PpsI GAGTC 2 cut(s) 727, 890
Ppu21I YACGTR 1 cut(s) 954
PpuMI RGGWCCY 1 cut(s) 217
PsiI TTATAA 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 3 cut(s) 802, 933, 1157
PspCI CACGTG 1 cut(s) 954
PspGI CCWGG 3 cut(s) 802, 933, 1157
PspN4I GGNNCC 1 cut(s) 939
PspPI GGNCC 5 cut(s) 108, 217, 454, 579, 620
PspPPI RGGWCCY 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 1106
PsuI RGATCY 3 cut(s) 593, 937, 1114
RsaI GTAC 4 cut(s) 260, 461, 700, 927
RsaNI GTAC 4 cut(s) 259, 460, 699, 926
RseI CAYNNNNRTG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 2 cut(s) 1014, 1038
SatI GCNGC 2 cut(s) 253, 565
Sau3AI GATC 7 cut(s) 593, 845, 877, 907, 937, 1114, 1212
Sau96I GGNCC 5 cut(s) 108, 217, 454, 579, 620
SchI GAGTC 2 cut(s) 727, 890
ScrFI CCNGG 3 cut(s) 804, 935, 1159
SfaNI GCATC 2 cut(s) 198, 1175
SfcI CTRYAG 1 cut(s) 1098
SinI GGWCC 4 cut(s) 217, 454, 579, 620
SmiMI CAYNNNNRTG 1 cut(s) 400
SpeI ACTAGT 2 cut(s) 701, 1088
Sse9I AATT 5 cut(s) 66, 502, 645, 830, 1015
SsiI CCGC 1 cut(s) 253
SspMI CTAG 7 cut(s) 702, 738, 852, 891, 959, 977, 1089
StyD4I CCNGG 3 cut(s) 802, 933, 1157
TaaI ACNGT 2 cut(s) 589, 1102
TaiI ACGT 3 cut(s) 844, 927, 956
TaqI TCGA 2 cut(s) 611, 1211
TasI AATT 5 cut(s) 66, 502, 645, 830, 1015
TatI WGTACW 2 cut(s) 258, 459
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 2 cut(s) 572, 632
Tru1I TTAA 2 cut(s) 1014, 1038
Tru9I TTAA 2 cut(s) 1014, 1038
TscAI CASTG 5 cut(s) 449, 574, 594, 767, 1222
TseFI GTSAC 2 cut(s) 337, 893
TseI GCWGC 1 cut(s) 564
Tsp45I GTSAC 2 cut(s) 337, 893
TspDTI ATGAA 8 cut(s) 78, 140, 158, 669, 696, 960, 1082, 1164
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 5 cut(s) 449, 574, 594, 767, 1222
VpaK11BI GGWCC 4 cut(s) 217, 454, 579, 620
XapI RAATTY 1 cut(s) 645
XbaI TCTAGA 1 cut(s) 958
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XspI CTAG 7 cut(s) 702, 738, 852, 891, 959, 977, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.