Rroxscaffold_2G00102400

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018461
Physical Location & Seq
Forward (+)
24545757 .. 24547608
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00102400.1

Sequence Viewer

Length: 1236 bp
ATGGATAACAAGAAATCCTCTTCGAGTGCTTTGATAGCATTACCTTCGCCAATTGAAGAAGATGAAATTGAATGGAAGAAGAGCTTACCATCTGGAGTGAAGTTTCGGCCCTCACATAAAGATTTATATCATTATCTAAATATGAAGGTTCATACTGGGCGCATTGATTTTGGTGGAGTTATTAGAAGCATCAATCTTTATATATATAAGCCCGAGGACCTAAGAGGTTTAGCATTTGATAATGATGATGGCCGCTTGTACTTCTTCACACCATTGCAAAAGAAGTTCACAAAGGGAAAAAAGACAAACAGAGTAACCGGACATGGTTTTTGGAAGGTCACGCAGAAGTCTGTAAAGATTATGGATGATGACAACAAGCCTATTATGACCAAAACACCTCTGTGTTACTTTAACTTTTGGGGCAAAAAGGGAAGGAAAACTCAGTGGCTCATGGACCAGTACAAGCTCATCAACGAGGGCAGTAATCCTGTAATGTTGAAGAATTGGGTTCTTTGTGTTGTCCACTACAAGCCAAGAGGGAATAATGATACAAACAAAAGAAGCAGCACTGATTCAAGGTCCGAGGCAGTGGGATGTTCTGCTCAAAACTCGATGATAGGTCCGTCTTCCGAATTCAATCCCCAAATTTCCTTTGGCAACAATAACTTCAATGTGGCTTCATCTTCTAACCCACAAGGTAGTTCATACCCAGAACCGGTGGCATTCCAGACTCCTAGTAACCCAAATAAGAGACAGCAGTGTTCTACTTCTTATGATGAGCCTTTGTGTATCACCTGCCCTGGTAATAACATTGCCTCTTATGCCCAATTTCCTAAACGTAGATTACCTAGTGCTAATGACACCCCCAACATTGATCAAAACCAAGCTAGTGACCCTTCTTTTGATCAAATGGTTTCTAACGTACTCAACCAGGATCCTGATGAATACACCTGTCTAGAGCCATTACTCAACCTAGATATAGACGATTACGAGTGGCAAGACGATATTTTTAATGATCTTTTTCCATCTGAACTTAACCCTCAAAATAGTAACCCTAATAGCATGAACCCTTCAACTACTACCACTAGTCATCCTACTGTCTCTGCCTCAGATCCACCTCCTCCCACCTTCAACTCTTCTGCCGATGAAAATGCCAGGATAGCATCTATGGCAAAAGTGGGTCAATCCAATGCCACAACAAGCTTATCGATCACTAAGGAAGGGAAGACAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

411

Amino Acids

46.06

Weight (kDa)

6.99

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 30 - 156 1.7e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018461)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g33050
rosa_laevigata RLG00000019978
rosa_multiflora Rmu_sc0004112.1_g000016
rosa_roxburghii Rroxscaffold_2G00102400
rosa_rugosa Rorug02G0374700
rosa_samantha Rh2AG427000 Rh2BG436300 Rh2CG413500 Rh2DG446200
rosa_wichuraiana Rw2G034980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 803
Acc36I ACCTGC 1 cut(s) 803
AciI CCGC 1 cut(s) 253
AclWI GGATC 3 cut(s) 929, 942, 1106
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 2 cut(s) 632, 645
AfaI GTAC 3 cut(s) 260, 461, 924
AfiI CCNNNNNNNGG 1 cut(s) 715
AgeI ACCGGT 1 cut(s) 715
AgsI TTSAA 8 cut(s) 56, 71, 499, 576, 637, 670, 1074, 1132
AhlI ACTAGT 1 cut(s) 1085
AjnI CCWGG 3 cut(s) 799, 930, 1154
AleI CACNNNNGTG 1 cut(s) 400
AluBI AGCT 4 cut(s) 84, 466, 887, 1203
AluI AGCT 4 cut(s) 84, 466, 887, 1203
Alw26I GTCTC 2 cut(s) 745, 1105
AlwI GGATC 3 cut(s) 929, 942, 1106
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 107, 250
ApeKI GCWGC 1 cut(s) 564
ApoI RAATTY 2 cut(s) 632, 645
ArsI GACNNNNNNTTYG 4 cut(s) 312, 344, 884, 916
AsiGI ACCGGT 1 cut(s) 715
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 5 cut(s) 108, 217, 454, 579, 620
AsuHPI GGTGA 1 cut(s) 784
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 4 cut(s) 217, 454, 579, 620
BamHI GGATCC 1 cut(s) 934
BbsI GAAGAC 2 cut(s) 618, 1232
BbvI GCAGC 1 cut(s) 576
BccI CCATC 3 cut(s) 97, 242, 1033
BcgI CGANNNNNNTGC 4 cut(s) 27, 61, 1133, 1167
BciT130I CCWGG 3 cut(s) 801, 932, 1156
BclI TGATCA 2 cut(s) 874, 904
BcoDI GTCTC 2 cut(s) 745, 1105
BcuI ACTAGT 1 cut(s) 1085
BfaI CTAG 6 cut(s) 735, 849, 888, 956, 974, 1086
BfuAI ACCTGC 1 cut(s) 803
BisI GCNGC 2 cut(s) 253, 565
BlsI GCNGC 2 cut(s) 254, 566
Bme1390I CCNGG 3 cut(s) 801, 932, 1156
Bme18I GGWCC 4 cut(s) 217, 454, 579, 620
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 5 cut(s) 108, 217, 454, 579, 620
BmiI GGNNCC 1 cut(s) 936
BmrFI CCNGG 3 cut(s) 801, 932, 1156
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 198, 1172
BmuI ACTGGG 1 cut(s) 165
BpiI GAAGAC 2 cut(s) 618, 1232
BpmI CTGGAG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 1208
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 213, 582, 799
BsaWI WCCGGW 2 cut(s) 317, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 715
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 2 cut(s) 160, 457
Bse3DI GCAATG 2 cut(s) 272, 810
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 3 cut(s) 801, 932, 1156
BseCI ATCGAT 1 cut(s) 1208
BseDI CCNNGG 3 cut(s) 213, 582, 799
BseGI GGATG 3 cut(s) 370, 599, 1090
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 715
BseMI GCAATG 2 cut(s) 272, 810
BseMII CTCAG 2 cut(s) 455, 1122
BseNI ACTGG 2 cut(s) 160, 457
BseRI GAGGAG 1 cut(s) 1110
BseXI GCAGC 1 cut(s) 576
BshFI GGCC 2 cut(s) 109, 252
BshTI ACCGGT 1 cut(s) 715
BshVI ATCGAT 1 cut(s) 1208
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 318, 716
BslI CCNNNNNNNGG 1 cut(s) 715
BsmAI GTCTC 2 cut(s) 745, 1105
BsmI GAATGC 1 cut(s) 722
BsnI GGCC 2 cut(s) 109, 252
BsoBI CYCGRG 1 cut(s) 212
Bsp143I GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
BspACI CCGC 1 cut(s) 253
BspANI GGCC 2 cut(s) 109, 252
BspCNI CTCAG 2 cut(s) 454, 1121
BspDI ATCGAT 1 cut(s) 1208
BspLI GGNNCC 1 cut(s) 936
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 3 cut(s) 929, 942, 1106
BspQI GCTCTTC 1 cut(s) 74
BsrDI GCAATG 2 cut(s) 272, 810
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 2 cut(s) 160, 457
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 3 cut(s) 213, 582, 799
BssMI GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
Bst2UI CCWGG 3 cut(s) 801, 932, 1156
Bst4CI ACNGT 1 cut(s) 1099
Bst6I CTCTTC 3 cut(s) 25, 74, 1141
BstDEI CTNAG 4 cut(s) 221, 441, 1108, 1215
BstF5I GGATG 3 cut(s) 370, 599, 1090
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 6 cut(s) 877, 907, 937, 1018, 1114, 1212
BstMAI GTCTC 2 cut(s) 745, 1105
BstMBI GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
BstMWI GCNNNNNNNGC 4 cut(s) 35, 821, 1160, 1169
BstNI CCWGG 3 cut(s) 801, 932, 1156
BstSCI CCNGG 3 cut(s) 799, 930, 1154
BstV1I GCAGC 1 cut(s) 576
BstV2I GAAGAC 2 cut(s) 618, 1232
BstX2I RGATCY 2 cut(s) 934, 1111
BstYI RGATCY 2 cut(s) 934, 1111
Bsu15I ATCGAT 1 cut(s) 1208
BsuRI GGCC 2 cut(s) 109, 252
BsuTUI ATCGAT 1 cut(s) 1208
BtsCI GGATG 3 cut(s) 370, 599, 1090
BtsI GCAGTG 2 cut(s) 594, 764
BtsIMutI CAGTG 4 cut(s) 449, 567, 594, 764
BveI ACCTGC 1 cut(s) 803
CfoI GCGC 1 cut(s) 162
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 5 cut(s) 108, 217, 454, 579, 620
ClaI ATCGAT 1 cut(s) 1208
Csp6I GTAC 3 cut(s) 259, 460, 923
CspAI ACCGGT 1 cut(s) 715
CviAII CATG 3 cut(s) 323, 451, 1063
CviQI GTAC 3 cut(s) 259, 460, 923
DdeI CTNAG 4 cut(s) 221, 441, 1108, 1215
DpnI GATC 6 cut(s) 876, 906, 936, 1017, 1113, 1211
DpnII GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 3 cut(s) 25, 74, 1141
EarI CTCTTC 3 cut(s) 25, 74, 1141
Eco47I GGWCC 4 cut(s) 217, 454, 579, 620
Eco88I CYCGRG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 3 cut(s) 799, 930, 1154
FaeI CATG 3 cut(s) 326, 454, 1066
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 3 cut(s) 322, 450, 1062
FbaI TGATCA 2 cut(s) 874, 904
Fnu4HI GCNGC 2 cut(s) 253, 565
FokI GGATG 3 cut(s) 377, 606, 1077
Fsp4HI GCNGC 2 cut(s) 253, 565
FspBI CTAG 6 cut(s) 735, 849, 888, 956, 974, 1086
GlaI GCGC 1 cut(s) 161
GluI GCNGC 2 cut(s) 253, 565
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 2 cut(s) 109, 252
HapII CCGG 2 cut(s) 318, 716
HhaI GCGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 326, 454, 1066
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 1201
HinfI GANTC 2 cut(s) 572, 730
HpaII CCGG 2 cut(s) 318, 716
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 2 cut(s) 288, 523
Hpy188I TCNGA 4 cut(s) 583, 631, 1030, 1111
Hpy188III TCNNGA 4 cut(s) 93, 727, 938, 956
Hpy8I GTNNAC 2 cut(s) 288, 523
HpyAV CCTTC 8 cut(s) 54, 139, 328, 426, 906, 1080, 1138, 1214
HpyCH4III ACNGT 1 cut(s) 1099
HpyCH4IV ACGT 2 cut(s) 838, 921
HpyCH4V TGCA 1 cut(s) 277
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 821, 1160, 1169
HpyF3I CTNAG 4 cut(s) 221, 441, 1108, 1215
HpySE526I ACGT 2 cut(s) 838, 921
Hsp92II CATG 3 cut(s) 326, 454, 1066
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 2 cut(s) 874, 904
Kzo9I GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
LguI GCTCTTC 1 cut(s) 74
Lsp1109I GCAGC 1 cut(s) 576
LweI GCATC 2 cut(s) 198, 1172
MaeI CTAG 6 cut(s) 735, 849, 888, 956, 974, 1086
MaeII ACGT 2 cut(s) 838, 921
MaeIII GTNAC 6 cut(s) 313, 337, 404, 737, 890, 1049
MalI GATC 6 cut(s) 876, 906, 936, 1017, 1113, 1211
MboI GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 2 cut(s) 934, 1111
MluCI AATT 6 cut(s) 51, 66, 502, 632, 645, 827
MlyI GAGTC 1 cut(s) 724
MseI TTAA 3 cut(s) 411, 1011, 1035
MslI CAYNNNNRTG 1 cut(s) 400
MspI CCGG 2 cut(s) 318, 716
MspR9I CCNGG 3 cut(s) 801, 932, 1156
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 3 cut(s) 801, 932, 1156
MwoI GCNNNNNNNGC 4 cut(s) 35, 821, 1160, 1169
NdeII GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
NlaIII CATG 3 cut(s) 326, 454, 1066
NlaIV GGNNCC 1 cut(s) 936
NmuCI GTSAC 2 cut(s) 337, 890
OliI CACNNNNGTG 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 803
PciSI GCTCTTC 1 cut(s) 74
PctI GAATGC 1 cut(s) 722
PfeI GAWTC 1 cut(s) 572
PinAI ACCGGT 1 cut(s) 715
PkrI GCNGC 2 cut(s) 254, 566
PleI GAGTC 1 cut(s) 724
PpsI GAGTC 1 cut(s) 724
PpuMI RGGWCCY 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 3 cut(s) 799, 930, 1154
PspGI CCWGG 3 cut(s) 799, 930, 1154
PspN4I GGNNCC 1 cut(s) 936
PspPI GGNCC 5 cut(s) 108, 217, 454, 579, 620
PspPPI RGGWCCY 1 cut(s) 217
PsuI RGATCY 2 cut(s) 934, 1111
RsaI GTAC 3 cut(s) 260, 461, 924
RsaNI GTAC 3 cut(s) 259, 460, 923
RseI CAYNNNNRTG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 411, 1011, 1035
SatI GCNGC 2 cut(s) 253, 565
Sau3AI GATC 6 cut(s) 874, 904, 934, 1015, 1111, 1209
Sau96I GGNCC 5 cut(s) 108, 217, 454, 579, 620
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 3 cut(s) 801, 932, 1156
SfaNI GCATC 2 cut(s) 198, 1172
SinI GGWCC 4 cut(s) 217, 454, 579, 620
SmiMI CAYNNNNRTG 1 cut(s) 400
SpeI ACTAGT 1 cut(s) 1085
Sse9I AATT 6 cut(s) 51, 66, 502, 632, 645, 827
SsiI CCGC 1 cut(s) 253
SspMI CTAG 6 cut(s) 735, 849, 888, 956, 974, 1086
StyD4I CCNGG 3 cut(s) 799, 930, 1154
TaaI ACNGT 1 cut(s) 1099
TaiI ACGT 2 cut(s) 841, 924
TaqI TCGA 3 cut(s) 23, 611, 1208
TasI AATT 6 cut(s) 51, 66, 502, 632, 645, 827
TatI WGTACW 2 cut(s) 258, 459
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 1 cut(s) 572
Tru1I TTAA 3 cut(s) 411, 1011, 1035
Tru9I TTAA 3 cut(s) 411, 1011, 1035
TscAI CASTG 4 cut(s) 449, 574, 594, 764
TseFI GTSAC 2 cut(s) 337, 890
TseI GCWGC 1 cut(s) 564
Tsp45I GTSAC 2 cut(s) 337, 890
TspDTI ATGAA 8 cut(s) 78, 140, 158, 669, 693, 957, 1079, 1161
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 4 cut(s) 449, 574, 594, 764
VpaK11BI GGWCC 4 cut(s) 217, 454, 579, 620
XapI RAATTY 2 cut(s) 632, 645
XbaI TCTAGA 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XspI CTAG 6 cut(s) 735, 849, 888, 956, 974, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.