RLG00000020612

4-hydroxyphenylpyruvate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010018
Physical Location & Seq
Reverse (-)
66715703 .. 66718685
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020612

Sequence Viewer

Length: 1155 bp
ATGGGCCACGAAACCGATAACCCCTTCAAGCTCGTCGGATTCTCCAACTTCGTTCGATCCAACCCCCGTTCCGACCGCTTCAAGGTCAACTGCTTCCACCACATCGAGTTCTGGTGCACCGACGCCACCAACGCAGCCCTCCGCTTCTCCTGGGGCCTCGGCATCCCCATGGTTGCCAAGTCTGACCTCTCCACTGGCAACCAAACCCACGCCTCCTATCTCCTCCGCTCCGGTGACCTCTCCATCCTCTTCACTGCCCCTTACTCCCCAACCATATCCCACCAGACCCGGCCCGCCTCCATCCCCACCTTCGACCACTCCGCCTCACGCGCCTTCTCCTCCAGACACGGCCTCGGAGTCCGCGCAGTAGCCGTCGAGGTCGCCGACGCGGACCTCGCCTTCCACACCAGCGTCTCCCACGGCGCTAAACCCATGTCCCAACCCATTCTCCTCGACAACCGCGTCACGATCGCCGAGGTCCATCTCTACGGCGACGTGGTTTTGCGGTACGTAAGCTACAAAGACCTAGCCCAGCTCGCCGACCCGAACCCGGACACGTGGTTCCTCCCCGGGTTCGAGCCGGTCCCCTCCTCCTTCCCCCTCGACTTCGGCTTCCGCCGTCTCGACCATGCCGTTGGCAACGTTCCCGACCTCTCCGCCGCCATGACTTACGTGAGAAGCTTCACCGGATTCCACGAGTTCTTGGAGTTCACGGCGGAGGACATCGGGACGAGCGAGAGCGGACTGAACTCGGCAGTTCTGGCCAACAACGACGAGACGGTGCTGCTTCCGATGAACGAGCCGGTGTACGGGACAAAGAGGAAGAGCCAGATTCAGACGTACCTGGAGCACAACGAAGGCGCCGGAGTCCAGCACCTCGCTCTGGTCAGCGAAGATATATTCAGGACCTTGAGGGAGATGAGGCAACGCAGCGGAATCGGAGGATTCGACTTCATGCCGTCGCCGCTGCCGACGTATTACAGGAATTTGAAGAAGAGGGCCGGCGATGTGTTGACGGATGAGCAGATCAAGGAGTGTGAGGAGTTGGGGATTTTGGTTGATAGAGATGACCAGGGGACTCTGCTTCAGATTTTCACAAAGCCTGTGGGAGATAGAAATGAGGTTCTTTCTCTTTCTCTTGTACCTCGTTATTAG

Protein Analysis

385

Amino Acids

42.73

Weight (kDa)

5.68

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 207 - 310 8.8e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 860
AccBSI CCGCTC 2 cut(s) 228, 741
AccII CGCG 4 cut(s) 330, 363, 389, 462
AclI AACGTT 1 cut(s) 642
AclWI GGATC 1 cut(s) 51
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 1 cut(s) 985
AcuI CTGAAG 1 cut(s) 1070
AcvI CACGTG 1 cut(s) 558
AcyI GRCGYC 2 cut(s) 123, 861
AfaI GTAC 4 cut(s) 509, 809, 842, 1143
AfiI CCNNNNNNNGG 4 cut(s) 82, 550, 809, 883
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 3 cut(s) 28, 82, 991
AjiI CACGTC 1 cut(s) 496
AjnI CCWGG 3 cut(s) 149, 843, 1071
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
AluBI AGCT 4 cut(s) 31, 516, 535, 681
AluI AGCT 4 cut(s) 31, 516, 535, 681
Alw21I GWGCWC 2 cut(s) 119, 852
Alw26I GTCTC 3 cut(s) 418, 626, 770
Alw44I GTGCAC 1 cut(s) 115
AlwI GGATC 1 cut(s) 51
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 6 cut(s) 4, 154, 290, 349, 762, 999
ApaLI GTGCAC 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 134, 784, 930, 967
ApoI RAATTY 1 cut(s) 985
AspLEI GCGC 4 cut(s) 332, 365, 425, 863
AspS9I GGNCC 8 cut(s) 4, 154, 291, 391, 478, 583, 906, 999
AsuC2I CCSGG 4 cut(s) 289, 551, 570, 571
AsuHPI GGTGA 2 cut(s) 245, 676
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 4 cut(s) 391, 478, 583, 906
BaeGI GKGCMC 1 cut(s) 119
BalI TGGCCA 1 cut(s) 764
BanI GGYRCC 1 cut(s) 860
BauI CACGAG 1 cut(s) 695
BbrPI CACGTG 1 cut(s) 558
Bbv12I GWGCWC 2 cut(s) 119, 852
BbvI GCAGC 4 cut(s) 146, 771, 942, 954
BccI CCATC 3 cut(s) 251, 308, 489
BceAI ACGGC 8 cut(s) 356, 364, 436, 505, 603, 617, 729, 943
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BciT130I CCWGG 3 cut(s) 151, 845, 1073
BcnI CCSGG 4 cut(s) 289, 551, 570, 571
BcoDI GTCTC 3 cut(s) 418, 626, 770
BfaI CTAG 1 cut(s) 527
BfoI RGCGCY 2 cut(s) 426, 864
BisI GCNGC 6 cut(s) 135, 660, 785, 931, 965, 968
BlsI GCNGC 6 cut(s) 136, 661, 786, 932, 966, 969
Bme1390I CCNGG 7 cut(s) 151, 289, 551, 570, 571, 845, 1073
Bme18I GGWCC 4 cut(s) 391, 478, 583, 906
BmeT110I CYCGRG 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 496
BmgT120I GGNCC 8 cut(s) 4, 154, 291, 391, 478, 583, 906, 999
BmiI GGNNCC 4 cut(s) 155, 563, 585, 862
BmrFI CCNGG 7 cut(s) 151, 289, 551, 570, 571, 845, 1073
BmsI GCATC 1 cut(s) 171
BpmI CTGGAG 2 cut(s) 325, 866
BpuEI CTTGAG 1 cut(s) 931
BpuMI CCSGG 4 cut(s) 289, 551, 570, 571
BsaAI YACGTR 3 cut(s) 511, 558, 673
BsaHI GRCGYC 2 cut(s) 123, 861
BsaJI CCNNGG 9 cut(s) 150, 157, 168, 352, 418, 474, 568, 569, 1072
BsaWI WCCGGW 2 cut(s) 230, 686
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 550, 809, 883
Bse118I RCCGGY 3 cut(s) 580, 802, 1001
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 3 cut(s) 151, 845, 1073
BseDI CCNNGG 9 cut(s) 150, 157, 168, 352, 418, 474, 568, 569, 1072
BseGI GGATG 4 cut(s) 162, 243, 300, 1024
BseLI CCNNNNNNNGG 4 cut(s) 82, 550, 809, 883
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 5 cut(s) 212, 328, 440, 580, 1055
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 4 cut(s) 146, 771, 942, 954
BseYI CCCAGC 1 cut(s) 531
Bsh1236I CGCG 4 cut(s) 330, 363, 389, 462
Bsh1285I CGRYCG 2 cut(s) 76, 471
BshFI GGCC 6 cut(s) 6, 156, 292, 351, 764, 1001
BshNI GGYRCC 1 cut(s) 860
BsiEI CGRYCG 2 cut(s) 76, 471
BsiHKAI GWGCWC 2 cut(s) 119, 852
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 9 cut(s) 231, 289, 551, 570, 581, 687, 803, 864, 1002
BslFI GGGAC 5 cut(s) 421, 569, 742, 826, 1090
BslI CCNNNNNNNGG 4 cut(s) 82, 550, 809, 883
BsmAI GTCTC 3 cut(s) 418, 626, 770
BsmBI CGTCTC 3 cut(s) 418, 626, 770
BsmFI GGGAC 5 cut(s) 421, 569, 742, 826, 1090
BsnI GGCC 6 cut(s) 6, 156, 292, 351, 764, 1001
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 119, 852
Bsp143I GATC 3 cut(s) 56, 468, 1026
Bsp19I CCATGG 1 cut(s) 168
BspANI GGCC 6 cut(s) 6, 156, 292, 351, 764, 1001
BspFNI CGCG 4 cut(s) 330, 363, 389, 462
BspLI GGNNCC 4 cut(s) 155, 563, 585, 862
BspPI GGATC 1 cut(s) 51
BspQI GCTCTTC 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 860
BsrBI CCGCTC 2 cut(s) 228, 741
BsrFI RCCGGY 3 cut(s) 580, 802, 1001
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 3 cut(s) 580, 802, 1001
BssECI CCNNGG 9 cut(s) 150, 157, 168, 352, 418, 474, 568, 569, 1072
BssMI GATC 3 cut(s) 56, 468, 1026
BssNI GRCGYC 2 cut(s) 123, 861
BssSI CACGAG 1 cut(s) 695
BssT1I CCWWGG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 695
Bst2UI CCWGG 3 cut(s) 151, 845, 1073
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 3 cut(s) 254, 818, 989
BstACI GRCGYC 2 cut(s) 123, 861
BstBAI YACGTR 3 cut(s) 511, 558, 673
BstC8I GCNNGC 3 cut(s) 294, 537, 1003
BstDSI CCRYGG 2 cut(s) 168, 418
BstEII GGTNACC 1 cut(s) 233
BstF5I GGATG 4 cut(s) 162, 243, 300, 1024
BstFNI CGCG 4 cut(s) 330, 363, 389, 462
BstH2I RGCGCY 2 cut(s) 426, 864
BstHHI GCGC 4 cut(s) 332, 365, 425, 863
BstKTI GATC 3 cut(s) 59, 471, 1029
BstMAI GTCTC 3 cut(s) 418, 626, 770
BstMBI GATC 3 cut(s) 56, 468, 1026
BstMCI CGRYCG 2 cut(s) 76, 471
BstMWI GCNNNNNNNGC 6 cut(s) 131, 329, 395, 536, 761, 964
BstNI CCWGG 3 cut(s) 151, 845, 1073
BstPI GGTNACC 1 cut(s) 233
BstSCI CCNGG 7 cut(s) 149, 287, 549, 568, 569, 843, 1071
BstSLI GKGCMC 1 cut(s) 119
BstSNI TACGTA 1 cut(s) 511
BstUI CGCG 4 cut(s) 330, 363, 389, 462
BstV1I GCAGC 4 cut(s) 146, 771, 942, 954
BstXI CCANNNNNNTGG 1 cut(s) 635
BsuRI GGCC 6 cut(s) 6, 156, 292, 351, 764, 1001
BtgI CCRYGG 2 cut(s) 168, 418
BtgZI GCGATG 1 cut(s) 1020
BtrI CACGTC 1 cut(s) 496
BtsCI GGATG 4 cut(s) 162, 243, 300, 1024
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 192, 252
Cac8I GCNNGC 3 cut(s) 294, 537, 1003
CfoI GCGC 4 cut(s) 332, 365, 425, 863
Cfr10I RCCGGY 3 cut(s) 580, 802, 1001
Cfr13I GGNCC 8 cut(s) 4, 154, 291, 391, 478, 583, 906, 999
Cfr9I CCCGGG 1 cut(s) 569
CseI GACGC 4 cut(s) 131, 395, 400, 451
Csp6I GTAC 4 cut(s) 508, 808, 841, 1142
CspCI CAANNNNNGTGG 2 cut(s) 1086, 1121
CviAII CATG 5 cut(s) 169, 433, 629, 664, 955
CviQI GTAC 4 cut(s) 508, 808, 841, 1142
DinI GGCGCC 1 cut(s) 862
DpnI GATC 3 cut(s) 58, 470, 1028
DpnII GATC 3 cut(s) 56, 468, 1026
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 3 cut(s) 254, 818, 989
EarI CTCTTC 3 cut(s) 254, 818, 989
EciI GGCGGA 4 cut(s) 310, 605, 646, 731
Eco105I TACGTA 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 168
Eco47I GGWCC 4 cut(s) 391, 478, 583, 906
Eco57I CTGAAG 1 cut(s) 1070
Eco72I CACGTG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 569
Eco91I GGTNACC 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 154, 906
EcoO65I GGTNACC 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 149, 843, 1071
EcoT14I CCWWGG 1 cut(s) 168
EgeI GGCGCC 1 cut(s) 862
EheI GGCGCC 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 168
Esp3I CGTCTC 3 cut(s) 418, 626, 770
FaeI CATG 5 cut(s) 172, 436, 632, 667, 958
FaiI YATR 7 cut(s) 170, 275, 434, 630, 665, 899, 956
FaqI GGGAC 5 cut(s) 421, 569, 742, 826, 1090
FatI CATG 5 cut(s) 168, 432, 628, 663, 954
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 6 cut(s) 135, 660, 785, 931, 965, 968
FokI GGATG 4 cut(s) 149, 230, 287, 1031
Fsp4HI GCNGC 6 cut(s) 135, 660, 785, 931, 965, 968
FspBI CTAG 1 cut(s) 527
GlaI GCGC 4 cut(s) 331, 364, 424, 862
GluI GCNGC 6 cut(s) 135, 660, 785, 931, 965, 968
GsaI CCCAGC 1 cut(s) 535
GsuI CTGGAG 2 cut(s) 325, 866
HaeII RGCGCY 2 cut(s) 426, 864
HaeIII GGCC 6 cut(s) 6, 156, 292, 351, 764, 1001
HapII CCGG 9 cut(s) 231, 289, 551, 570, 581, 687, 803, 864, 1002
HgaI GACGC 4 cut(s) 131, 395, 400, 451
HhaI GCGC 4 cut(s) 332, 365, 425, 863
Hin1I GRCGYC 2 cut(s) 123, 861
Hin1II CATG 5 cut(s) 172, 436, 632, 667, 958
Hin6I GCGC 4 cut(s) 330, 363, 423, 861
HinP1I GCGC 4 cut(s) 330, 363, 423, 861
HincII GTYRAC 2 cut(s) 88, 1014
HindII GTYRAC 2 cut(s) 88, 1014
HindIII AAGCTT 1 cut(s) 679
HinfI GANTC 8 cut(s) 39, 357, 690, 832, 867, 936, 945, 1078
HpaII CCGG 9 cut(s) 231, 289, 551, 570, 581, 687, 803, 864, 1002
HphI GGTGA 2 cut(s) 245, 676
Hpy166II GTNNAC 5 cut(s) 88, 117, 711, 808, 1014
Hpy188I TCNGA 8 cut(s) 38, 73, 184, 356, 792, 837, 941, 1089
Hpy188III TCNNGA 6 cut(s) 342, 466, 623, 647, 727, 904
Hpy8I GTNNAC 5 cut(s) 88, 117, 711, 808, 1014
Hpy99I CGWCG 8 cut(s) 38, 125, 377, 389, 497, 776, 964, 976
HpyAV CCTTC 6 cut(s) 34, 319, 343, 409, 604, 851
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 7 cut(s) 495, 510, 557, 642, 672, 839, 974
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 329, 395, 536, 761, 964
HpySE526I ACGT 7 cut(s) 495, 510, 557, 642, 672, 839, 974
Hsp92I GRCGYC 2 cut(s) 123, 861
Hsp92II CATG 5 cut(s) 172, 436, 632, 667, 958
HspAI GCGC 4 cut(s) 330, 363, 423, 861
KasI GGCGCC 1 cut(s) 860
KroI GCCGGC 1 cut(s) 1001
KroNI GCCGGC 1 cut(s) 1003
Kzo9I GATC 3 cut(s) 56, 468, 1026
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 2 cut(s) 233, 847
Lsp1109I GCAGC 4 cut(s) 146, 771, 942, 954
LweI GCATC 1 cut(s) 171
MaeI CTAG 1 cut(s) 527
MaeII ACGT 7 cut(s) 495, 510, 557, 642, 672, 839, 974
MaeIII GTNAC 2 cut(s) 233, 463
MalI GATC 3 cut(s) 58, 470, 1028
MbiI CCGCTC 2 cut(s) 228, 741
MboI GATC 3 cut(s) 56, 468, 1026
MboII GAAGA 5 cut(s) 241, 835, 905, 1003, 1006
MhlI GDGCHC 2 cut(s) 119, 852
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 1 cut(s) 985
MluNI TGGCCA 1 cut(s) 764
Mly113I GGCGCC 1 cut(s) 861
MlyI GAGTC 3 cut(s) 366, 876, 1072
MmeI TCCRAC 4 cut(s) 16, 69, 84, 96
Mox20I TGGCCA 1 cut(s) 764
MroNI GCCGGC 1 cut(s) 1001
MscI TGGCCA 1 cut(s) 764
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 764
MspA1I CMGCKG 2 cut(s) 933, 967
MspI CCGG 9 cut(s) 231, 289, 551, 570, 581, 687, 803, 864, 1002
MspR9I CCNGG 7 cut(s) 151, 289, 551, 570, 571, 845, 1073
MvaI CCWGG 3 cut(s) 151, 845, 1073
MvnI CGCG 4 cut(s) 330, 363, 389, 462
MwoI GCNNNNNNNGC 6 cut(s) 131, 329, 395, 536, 761, 964
NaeI GCCGGC 1 cut(s) 1003
NarI GGCGCC 1 cut(s) 861
NciI CCSGG 4 cut(s) 289, 551, 570, 571
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 56, 468, 1026
NgoMIV GCCGGC 1 cut(s) 1001
NlaIII CATG 5 cut(s) 172, 436, 632, 667, 958
NlaIV GGNNCC 4 cut(s) 155, 563, 585, 862
NmeAIII GCCGAG 3 cut(s) 138, 499, 731
NmuCI GTSAC 2 cut(s) 233, 463
PciSI GCTCTTC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 6 cut(s) 381, 459, 630, 732, 945, 968
PdiI GCCGGC 1 cut(s) 1003
PfeI GAWTC 5 cut(s) 39, 690, 832, 936, 945
PkrI GCNGC 6 cut(s) 136, 661, 786, 932, 966, 969
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 3 cut(s) 365, 875, 1072
PluTI GGCGCC 1 cut(s) 864
PmaCI CACGTG 1 cut(s) 558
PmlI CACGTG 1 cut(s) 558
PpsI GAGTC 3 cut(s) 365, 875, 1072
Ppu21I YACGTR 3 cut(s) 511, 558, 673
PpuMI RGGWCCY 1 cut(s) 906
Psp1406I AACGTT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 906
Psp6I CCWGG 3 cut(s) 149, 843, 1071
PspCI CACGTG 1 cut(s) 558
PspEI GGTNACC 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 3 cut(s) 149, 843, 1071
PspN4I GGNNCC 4 cut(s) 155, 563, 585, 862
PspPI GGNCC 8 cut(s) 4, 154, 291, 391, 478, 583, 906, 999
PspPPI RGGWCCY 1 cut(s) 906
PvuI CGATCG 1 cut(s) 471
RsaI GTAC 4 cut(s) 509, 809, 842, 1143
RsaNI GTAC 4 cut(s) 508, 808, 841, 1142
RseI CAYNNNNRTG 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 818
SatI GCNGC 6 cut(s) 135, 660, 785, 931, 965, 968
Sau3AI GATC 3 cut(s) 56, 468, 1026
Sau96I GGNCC 8 cut(s) 4, 154, 291, 391, 478, 583, 906, 999
SchI GAGTC 3 cut(s) 366, 876, 1072
ScrFI CCNGG 7 cut(s) 151, 289, 551, 570, 571, 845, 1073
SduI GDGCHC 2 cut(s) 119, 852
SfaNI GCATC 1 cut(s) 171
SfoI GGCGCC 1 cut(s) 862
SinI GGWCC 4 cut(s) 391, 478, 583, 906
SmaI CCCGGG 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 910
SmoI CTYRAG 1 cut(s) 910
SnaBI TACGTA 1 cut(s) 511
Sse9I AATT 1 cut(s) 985
SspDI GGCGCC 1 cut(s) 860
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 7 cut(s) 149, 287, 549, 568, 569, 843, 1071
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 7 cut(s) 498, 513, 560, 645, 675, 842, 977
TaqI TCGA 9 cut(s) 55, 105, 312, 375, 453, 576, 603, 624, 948
TasI AATT 1 cut(s) 985
TauI GCSGC 2 cut(s) 662, 967
TfiI GAWTC 5 cut(s) 39, 690, 832, 936, 945
TscAI CASTG 2 cut(s) 199, 259
TseFI GTSAC 2 cut(s) 233, 463
TseI GCWGC 4 cut(s) 134, 784, 930, 967
Tsp45I GTSAC 2 cut(s) 233, 463
TspDTI ATGAA 2 cut(s) 809, 943
TspGWI ACGGA 1 cut(s) 1031
TspMI CCCGGG 1 cut(s) 569
TspRI CASTG 2 cut(s) 199, 259
VneI GTGCAC 1 cut(s) 115
VpaK11BI GGWCC 4 cut(s) 391, 478, 583, 906
XapI RAATTY 1 cut(s) 985
XmaI CCCGGG 1 cut(s) 569
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.