RLG00000021219

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018496
Physical Location & Seq
Reverse (-)
73051236 .. 73051565
330 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021219

Sequence Viewer

Length: 330 bp
ATGAAGTCTCTCCTCTCTGATTTCCGCTCTTGCTACCGCGCGGGGACGTCGAAGCGGCGACCACCTTCTCCGGCTCAGGAGGCGCGTACTGATCACGCGCCGCCCTCGCGGAGGTTGAGGTCCCCGAAATCGGAGTCCTCTGCAGGTACGCAGTGGCGGCCGGCTCTCAACGCAATCTCGGAGGATGGGGATGCTACTCCTAATAGTTCTAAAGGCAGATCATCATCCAAGACTACAGTGGCCCAGTCCCGCAGTATTGCTAGCATTGACCAAGTCAACGCGCCGGTGATGATTCCGTCGGCTTACTGGGCGACGCCGTTTATGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

11.84

Weight (kDa)

10.6

Isoelectric Point (pI)

90.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018496)

Species Orthologous Gene IDs
malus_domestica MD09G1103100.v1.1
prunus_persica Prupe.3G222700_v2.0.a1
pyrus_communis pycom09g02630
rosa_laevigata RLG00000021219
rosa_roxburghii Rroxscaffold_2G00089770
rosa_rugosa Rorug02G0483500
rosa_samantha Rh2AG548800 Rh2BG562500 Rh2CG533100 Rh2DG571200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 134
AccBSI CCGCTC 1 cut(s) 27
AccII CGCG 6 cut(s) 39, 41, 85, 98, 109, 281
AciI CCGC 8 cut(s) 25, 37, 41, 55, 101, 109, 157, 250
AcoI YGGCCR 1 cut(s) 158
AcyI GRCGYC 2 cut(s) 47, 314
AfaI GTAC 2 cut(s) 88, 148
AfiI CCNNNNNNNGG 2 cut(s) 111, 130
Alw26I GTCTC 1 cut(s) 12
AoxI GGCC 2 cut(s) 158, 240
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AspLEI GCGC 4 cut(s) 41, 85, 100, 283
AspS9I GGNCC 2 cut(s) 120, 241
AsuHPI GGTGA 1 cut(s) 298
AsuNHI GCTAGC 1 cut(s) 260
AvaII GGWCC 1 cut(s) 120
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 301
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 2 cut(s) 141, 234
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 3 cut(s) 56, 101, 158
BlsI GCNGC 3 cut(s) 57, 102, 159
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 120, 241
BmiI GGNNCC 1 cut(s) 122
BmrI ACTGGG 2 cut(s) 238, 316
BmsI GCATC 1 cut(s) 181
BmtI GCTAGC 1 cut(s) 264
BmuI ACTGGG 2 cut(s) 238, 316
Bpu10I CCTNAGC 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 223
BsaHI GRCGYC 2 cut(s) 47, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 130
Bse118I RCCGGY 2 cut(s) 160, 283
Bse1I ACTGG 2 cut(s) 244, 311
Bse8I GATNNNNATC 1 cut(s) 223
BseGI GGATG 3 cut(s) 190, 196, 224
BseJI GATNNNNATC 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 111, 130
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 244, 311
BseX3I CGGCCG 1 cut(s) 158
Bsh1236I CGCG 6 cut(s) 39, 41, 85, 98, 109, 281
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 160, 242
BsiEI CGRYCG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 71, 161, 284
BslFI GGGAC 3 cut(s) 58, 106, 232
BslI CCNNNNNNNGG 2 cut(s) 111, 130
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 58, 106, 232
BsnI GGCC 2 cut(s) 160, 242
Bsp143I GATC 2 cut(s) 91, 218
BspACI CCGC 8 cut(s) 25, 37, 41, 55, 101, 109, 157, 250
BspANI GGCC 2 cut(s) 160, 242
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 6 cut(s) 39, 41, 85, 98, 109, 281
BspLI GGNNCC 1 cut(s) 122
BspMAI CTGCAG 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 134
BspOI GCTAGC 1 cut(s) 264
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 160, 283
BsrI ACTGG 2 cut(s) 244, 311
BssAI RCCGGY 2 cut(s) 160, 283
BssMI GATC 2 cut(s) 91, 218
BssNI GRCGYC 2 cut(s) 47, 314
Bst4CI ACNGT 1 cut(s) 238
BstACI GRCGYC 2 cut(s) 47, 314
BstC8I GCNNGC 2 cut(s) 162, 262
BstDEI CTNAG 1 cut(s) 75
BstENI CCTNNNNNAGG 1 cut(s) 109
BstF5I GGATG 3 cut(s) 190, 196, 224
BstFNI CGCG 6 cut(s) 39, 41, 85, 98, 109, 281
BstHHI GCGC 4 cut(s) 41, 85, 100, 283
BstKTI GATC 2 cut(s) 94, 221
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 91, 218
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 5 cut(s) 80, 106, 157, 170, 308
BstSFI CTRYAG 2 cut(s) 141, 234
BstUI CGCG 6 cut(s) 39, 41, 85, 98, 109, 281
BstZI CGGCCG 1 cut(s) 158
BsuRI GGCC 2 cut(s) 160, 242
BtsCI GGATG 3 cut(s) 190, 196, 224
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 2 cut(s) 158, 243
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 2 cut(s) 162, 262
CfoI GCGC 4 cut(s) 41, 85, 100, 283
Cfr10I RCCGGY 2 cut(s) 160, 283
Cfr13I GGNCC 2 cut(s) 120, 241
CseI GACGC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 87, 147
CviJI RGCY 5 cut(s) 74, 160, 164, 242, 302
CviKI_1 RGCY 5 cut(s) 74, 160, 164, 242, 302
CviQI GTAC 2 cut(s) 87, 147
DdeI CTNAG 1 cut(s) 75
DpnI GATC 2 cut(s) 93, 220
DpnII GATC 2 cut(s) 91, 218
EaeI YGGCCR 1 cut(s) 158
EagI CGGCCG 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 120
Eco52I CGGCCG 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 120
FaiI YATR 1 cut(s) 323
FaqI GGGAC 3 cut(s) 58, 106, 232
FauI CCCGC 2 cut(s) 34, 257
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 3 cut(s) 56, 101, 158
FokI GGATG 3 cut(s) 197, 203, 211
Fsp4HI GCNGC 3 cut(s) 56, 101, 158
FspBI CTAG 1 cut(s) 261
GlaI GCGC 4 cut(s) 40, 84, 99, 282
GluI GCNGC 3 cut(s) 56, 101, 158
HaeIII GGCC 2 cut(s) 160, 242
HapII CCGG 3 cut(s) 71, 161, 284
HgaI GACGC 1 cut(s) 322
HhaI GCGC 4 cut(s) 41, 85, 100, 283
Hin1I GRCGYC 2 cut(s) 47, 314
Hin6I GCGC 4 cut(s) 39, 83, 98, 281
HinP1I GCGC 4 cut(s) 39, 83, 98, 281
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 2 cut(s) 134, 292
HpaII CCGG 3 cut(s) 71, 161, 284
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 19, 133, 181, 329
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 3 cut(s) 52, 301, 316
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 106, 157, 170, 308
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 1 cut(s) 47
Hsp92I GRCGYC 2 cut(s) 47, 314
HspAI GCGC 4 cut(s) 39, 83, 98, 281
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 91, 218
LpnPI CCDG 7 cut(s) 62, 84, 129, 174, 257, 292, 297
LweI GCATC 1 cut(s) 181
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 47
MalI GATC 2 cut(s) 93, 220
MbiI CCGCTC 1 cut(s) 27
MboI GATC 2 cut(s) 91, 218
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 7 cut(s) 23, 73, 105, 111, 115, 148, 175
MroNI GCCGGC 1 cut(s) 160
MspI CCGG 3 cut(s) 71, 161, 284
MvnI CGCG 6 cut(s) 39, 41, 85, 98, 109, 281
MwoI GCNNNNNNNGC 5 cut(s) 80, 106, 157, 170, 308
NaeI GCCGGC 1 cut(s) 162
NdeII GATC 2 cut(s) 91, 218
NgoMIV GCCGGC 1 cut(s) 160
NheI GCTAGC 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 122
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 292
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 3 cut(s) 57, 102, 159
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 2 cut(s) 120, 241
PspPPI RGGWCCY 1 cut(s) 120
PstI CTGCAG 1 cut(s) 145
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 2 cut(s) 88, 148
RsaNI GTAC 2 cut(s) 87, 147
SatI GCNGC 3 cut(s) 56, 101, 158
Sau3AI GATC 2 cut(s) 91, 218
Sau96I GGNCC 2 cut(s) 120, 241
SchI GAGTC 1 cut(s) 143
SetI ASST 5 cut(s) 50, 67, 116, 122, 148
SfaNI GCATC 1 cut(s) 181
SfcI CTRYAG 2 cut(s) 141, 234
SgrAI CRCCGGYG 1 cut(s) 283
SinI GGWCC 1 cut(s) 120
SsiI CCGC 8 cut(s) 25, 37, 41, 55, 101, 109, 157, 250
SspMI CTAG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 50
TaqI TCGA 1 cut(s) 50
TauI GCSGC 3 cut(s) 58, 103, 160
TfiI GAWTC 1 cut(s) 292
TscAI CASTG 2 cut(s) 158, 243
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 2 cut(s) 158, 243
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 120
XagI CCTNNNNNAGG 1 cut(s) 109
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XspI CTAG 1 cut(s) 261
ZraI GACGTC 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.