RLG00000021389

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018065
Physical Location & Seq
Reverse (-)
74778542 .. 74779278
737 bp
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UTR
Exon/CDS
Intron
RLM00000021389

Sequence Viewer

Length: 501 bp
ATGGCCACCAAGTTCGTCCTCGTCTCGCTTCTCCTCCTCCAGCTTGCCGTAACTGCTTTCTCCGGCGTCGCTCAACAGAACCCTCACCACCAACCACACGGTAAACACAAGACCGCACATGAGCCAGGGGGTCCAGCAGGGGGGATTCCAAAGCCAGGAAGTAAACCACAAGTTCCGTTCGGAGGAGCGGATGAAGCACCTCACAATGATGTACCTGCATCCAGTGGAGGAGCTACCGCCGGCGGCCCTGGCAAGAAGTGGTGCATAGCCAAAACTAGTATTCAGAAAAGCCTTTTGAAGAAGGACTTTGATGGCTTGTGCAAAGAGGTCGATTGCTCGCCTACAGACGGAAAAGGTTTATGCTATACTGAGTCCATCCACGCCAAAGCGTCCTTCGCCATGAATCTAAAATATCAAAAGAACGGTAAGAAGGATGCTGACTGCAACTACGAGGGAAGAGCAGAGATTGTCACCAAAGACCCAAGTACGTCATCAACATAA

Protein Analysis

167

Amino Acids

17.34

Weight (kDa)

8.93

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 86 - 154 7.1e-13 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018065)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45460
rosa_chinensis RchiOBHm_Chr2g0163301
rosa_laevigata RLG00000021389
rosa_multiflora Rmu_sc0002045.1_g000007
rosa_roxburghii Rroxscaffold_2G00087400
rosa_rugosa Rorug02G0502100
rosa_samantha Rh2AG568900 Rh2BG581000 Rh2CG550600 Rh2DG590900
rosa_wichuraiana Rw2G047070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 223
AccBSI CCGCTC 1 cut(s) 188
AciI CCGC 4 cut(s) 114, 188, 237, 243
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 213, 487
AfiI CCNNNNNNNGG 4 cut(s) 140, 155, 182, 347
AgsI TTSAA 1 cut(s) 298
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 3 cut(s) 124, 154, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AluBI AGCT 2 cut(s) 43, 233
AluI AGCT 2 cut(s) 43, 233
Alw26I GTCTC 1 cut(s) 28
AoxI GGCC 2 cut(s) 3, 244
AspS9I GGNCC 2 cut(s) 131, 245
AsuHPI GGTGA 2 cut(s) 77, 463
AvaII GGWCC 1 cut(s) 131
BalI TGGCCA 1 cut(s) 5
BccI CCATC 2 cut(s) 305, 383
BceAI ACGGC 1 cut(s) 32
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 3 cut(s) 126, 156, 249
BcoDI GTCTC 1 cut(s) 28
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
Bme1390I CCNGG 3 cut(s) 126, 156, 249
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 131, 245
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 3 cut(s) 126, 156, 249
BmsI GCATC 2 cut(s) 227, 424
BpmI CTGGAG 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 125, 247
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 155, 182, 347
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 222
BseBI CCWGG 3 cut(s) 126, 156, 249
BseDI CCNNGG 2 cut(s) 125, 247
BseGI GGATG 4 cut(s) 196, 218, 375, 439
BseLI CCNNNNNNNGG 4 cut(s) 140, 155, 182, 347
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 222
BseRI GAGGAG 4 cut(s) 23, 26, 198, 243
BshFI GGCC 2 cut(s) 5, 246
BsiSI CCGG 2 cut(s) 63, 240
BslI CCNNNNNNNGG 4 cut(s) 140, 155, 182, 347
BsmAI GTCTC 1 cut(s) 28
BsmBI CGTCTC 1 cut(s) 28
BsnI GGCC 2 cut(s) 5, 246
BspACI CCGC 4 cut(s) 114, 188, 237, 243
BspANI GGCC 2 cut(s) 5, 246
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 132
BspMI ACCTGC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 188
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 1 cut(s) 222
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 125, 247
BssNI GRCGYC 1 cut(s) 66
Bst2UI CCWGG 3 cut(s) 126, 156, 249
Bst4CI ACNGT 2 cut(s) 101, 425
Bst6I CTCTTC 1 cut(s) 451
BstACI GRCGYC 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 45, 241, 338
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 4 cut(s) 196, 218, 375, 439
BstMAI GTCTC 1 cut(s) 28
BstMWI GCNNNNNNNGC 4 cut(s) 53, 194, 249, 395
BstNI CCWGG 3 cut(s) 126, 156, 249
BstSCI CCNGG 3 cut(s) 124, 154, 247
BstSFI CTRYAG 1 cut(s) 342
BsuRI GGCC 2 cut(s) 5, 246
BtsCI GGATG 4 cut(s) 196, 218, 375, 439
BtsIMutI CAGTG 1 cut(s) 229
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 3 cut(s) 45, 241, 338
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 131, 245
CseI GACGC 2 cut(s) 55, 378
Csp6I GTAC 2 cut(s) 212, 486
CviAII CATG 2 cut(s) 119, 400
CviJI RGCY 9 cut(s) 5, 43, 124, 154, 233, 246, 269, 291, 315
CviKI_1 RGCY 9 cut(s) 5, 43, 124, 154, 233, 246, 269, 291, 315
CviQI GTAC 2 cut(s) 212, 486
DdeI CTNAG 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 124, 154, 247
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 2 cut(s) 122, 403
FaiI YATR 6 cut(s) 120, 266, 361, 366, 401, 499
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 2 cut(s) 118, 399
Fnu4HI GCNGC 1 cut(s) 244
FokI GGATG 4 cut(s) 203, 205, 362, 446
Fsp4HI GCNGC 1 cut(s) 244
FspBI CTAG 1 cut(s) 276
GluI GCNGC 1 cut(s) 244
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 2 cut(s) 5, 246
HapII CCGG 2 cut(s) 63, 240
HgaI GACGC 2 cut(s) 55, 378
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 122, 403
HinfI GANTC 3 cut(s) 145, 371, 403
HpaII CCGG 2 cut(s) 63, 240
HphI GGTGA 2 cut(s) 77, 463
Hpy166II GTNNAC 2 cut(s) 104, 164
Hpy188I TCNGA 2 cut(s) 182, 285
Hpy8I GTNNAC 2 cut(s) 104, 164
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 295, 403, 424
HpyCH4III ACNGT 2 cut(s) 101, 425
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 4 cut(s) 218, 264, 321, 444
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 194, 249, 395
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 488
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 122, 403
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
LguI GCTCTTC 1 cut(s) 451
LmnI GCTCC 2 cut(s) 185, 230
LweI GCATC 2 cut(s) 227, 424
MaeI CTAG 1 cut(s) 276
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 49, 469
MbiI CCGCTC 1 cut(s) 188
MboII GAAGA 2 cut(s) 310, 468
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 9 cut(s) 29, 44, 47, 93, 176, 210, 221, 319, 445
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 239
MroNI GCCGGC 1 cut(s) 239
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 63, 240
MspR9I CCNGG 3 cut(s) 126, 156, 249
MvaI CCWGG 3 cut(s) 126, 156, 249
MwoI GCNNNNNNNGC 4 cut(s) 53, 194, 249, 395
NaeI GCCGGC 1 cut(s) 241
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 122, 403
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 451
PdiI GCCGGC 1 cut(s) 241
PfeI GAWTC 2 cut(s) 145, 403
PkrI GCNGC 1 cut(s) 245
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 124, 154, 247
PspGI CCWGG 3 cut(s) 124, 154, 247
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 2 cut(s) 131, 245
RsaI GTAC 2 cut(s) 213, 487
RsaNI GTAC 2 cut(s) 212, 486
RseI CAYNNNNRTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 451
SatI GCNGC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 131, 245
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 3 cut(s) 126, 156, 249
SetI ASST 7 cut(s) 45, 202, 217, 235, 330, 358, 491
SfaNI GCATC 2 cut(s) 227, 424
SfcI CTRYAG 1 cut(s) 342
SgrAI CRCCGGYG 1 cut(s) 239
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 207
SpeI ACTAGT 1 cut(s) 275
SsiI CCGC 4 cut(s) 114, 188, 237, 243
SspMI CTAG 1 cut(s) 276
StyD4I CCNGG 3 cut(s) 124, 154, 247
TaaI ACNGT 2 cut(s) 101, 425
TaiI ACGT 1 cut(s) 491
TaqI TCGA 1 cut(s) 330
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 2 cut(s) 145, 403
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 469
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 2 cut(s) 207, 416
TspGWI ACGGA 2 cut(s) 165, 363
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 131
XspI CTAG 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.