RLG00000022358

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019562
Physical Location & Seq
Forward (+)
82814640 .. 82817425
2786 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022358

Sequence Viewer

Length: 696 bp
ATGGCGGACTGGGGCCCAGTACTGATTGGGGTTGTGCTGTTTATTCTGCTCCAACCGGGACTCCTCTTCTCGTTGCCGGGAAACGGCAAGCAGGTCGAGTTTGGGAGCATGAAGACCAACGATGCCGATTTAGAGGAGAGATGTATTGACAGTCTCTTGCAAGTGGTCAATGATCTGGGAATCATTTTTAGTTATTGTTTTAGGATAGCAATCCCGCTTGTCATCATGTTTCCGAACTCCATCACCCTCGTCCTTGCTGGTAATTGGAATAGCAGCCCCGGCGACAAGAGAATGAACAAGACCACCGCAACAACGACCGGCCCCCAATCAGCACTCATCATTCCCTCAGCTCTCGCTCCGCTTACAAAATCAAGGAAAGAAAAGCTAAAAGGGCACTACACCAAGGATGTAGGTGCTAGCACGGTAACACCACTACTAGTGCTACACATCTTTTCAACTTTTAGAGTAGTAGTAAAAATGGCGGATTGGGGGCCGGTGTTGATAGCGGTGGTGCTGTTCGTACTGTTAACTCCAGGATTGCTGTTTCAGCTACCCGGGAACAGCCGAGTGGTGGAGTTTAACAACATGCAGACGAGTGGAGTTTCCATTTTGGTGCACACCATCATTTACTTTGCACTCATCACTGTCTTTCTCATAGCTATTGGTGTTCACATCTACACCGGCGGCGGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

232

Amino Acids

24.94

Weight (kDa)

8.93

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 41 2.3e-17 Protein of unknown function (DUF3339)
DUF3339 PF11820 160 - 226 2.9e-35 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019562)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g53740
malus_domestica MD09G1009200.v1.1 MD17G1001200.v1.1
prunus_persica Prupe.3G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0175821
rosa_laevigata RLG00000022358
rosa_wichuraiana Rw2G054690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 82
AciI CCGC 9 cut(s) 5, 215, 306, 359, 482, 506, 684, 687, 690
AfaI GTAC 2 cut(s) 21, 522
AfiI CCNNNNNNNGG 2 cut(s) 83, 571
AgsI TTSAA 1 cut(s) 456
AhlI ACTAGT 1 cut(s) 436
AjnI CCWGG 1 cut(s) 532
AluBI AGCT 4 cut(s) 350, 385, 550, 659
AluI AGCT 4 cut(s) 350, 385, 550, 659
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 1 cut(s) 158
Alw44I GTGCAC 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 554
AoxI GGCC 3 cut(s) 13, 319, 491
ApaI GGGCCC 1 cut(s) 17
ApaLI GTGCAC 1 cut(s) 614
ApeKI GCWGC 1 cut(s) 273
AspS9I GGNCC 4 cut(s) 13, 14, 320, 491
AsuC2I CCSGG 5 cut(s) 57, 78, 279, 555, 556
AsuHPI GGTGA 1 cut(s) 235
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 554
BaeGI GKGCMC 3 cut(s) 17, 396, 618
BanII GRGCYC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 618
BbvCI CCTCAGC 1 cut(s) 346
BbvI GCAGC 1 cut(s) 285
BccI CCATC 2 cut(s) 248, 629
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 534
BcnI CCSGG 5 cut(s) 57, 78, 279, 555, 556
BcoDI GTCTC 1 cut(s) 158
BcuI ACTAGT 1 cut(s) 436
BfaI CTAG 3 cut(s) 417, 437, 694
BfuAI ACCTGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 274, 685, 688, 691
BlsI GCNGC 4 cut(s) 275, 686, 689, 692
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 6 cut(s) 57, 78, 279, 534, 555, 556
BmeT110I CYCGRG 1 cut(s) 554
BmgT120I GGNCC 4 cut(s) 13, 14, 320, 491
BmiI GGNNCC 4 cut(s) 14, 15, 322, 492
BmrFI CCNGG 6 cut(s) 57, 78, 279, 534, 555, 556
BmrI ACTGGG 2 cut(s) 11, 19
BmsI GCATC 1 cut(s) 112
BmtI GCTAGC 1 cut(s) 420
BmuI ACTGGG 2 cut(s) 11, 19
BpiI GAAGAC 1 cut(s) 119
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 346
BpuMI CCSGG 5 cut(s) 57, 78, 279, 555, 556
BsaBI GATNNNNATC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 277, 402, 554
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 571
Bse118I RCCGGY 3 cut(s) 317, 493, 680
Bse1I ACTGG 2 cut(s) 14, 17
Bse8I GATNNNNATC 1 cut(s) 209
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 3 cut(s) 277, 402, 554
BseGI GGATG 1 cut(s) 412
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 83, 571
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 14, 17
BseRI GAGGAG 2 cut(s) 53, 149
BseSI GKGCMC 3 cut(s) 17, 396, 618
BseXI GCAGC 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 3 cut(s) 15, 321, 493
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 618
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 7 cut(s) 56, 77, 279, 318, 494, 555, 681
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 2 cut(s) 83, 571
BsmAI GTCTC 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 3 cut(s) 15, 321, 493
BsoBI CYCGRG 1 cut(s) 554
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 3 cut(s) 17, 396, 618
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 9 cut(s) 5, 215, 306, 359, 482, 506, 684, 687, 690
BspANI GGCC 3 cut(s) 15, 321, 493
BspCNI CTCAG 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 14, 15, 322, 492
BspMI ACCTGC 1 cut(s) 82
BspOI GCTAGC 1 cut(s) 420
BsrFI RCCGGY 3 cut(s) 317, 493, 680
BsrI ACTGG 2 cut(s) 14, 17
BssAI RCCGGY 3 cut(s) 317, 493, 680
BssECI CCNNGG 3 cut(s) 277, 402, 554
BssMI GATC 1 cut(s) 172
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 4 cut(s) 152, 424, 525, 646
Bst6I CTCTTC 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 89, 418
BstDEI CTNAG 1 cut(s) 346
BstF5I GGATG 1 cut(s) 412
BstKTI GATC 1 cut(s) 175
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 1 cut(s) 172
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 4 cut(s) 279, 391, 547, 690
BstNI CCWGG 1 cut(s) 534
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 6 cut(s) 55, 76, 277, 532, 553, 554
BstSLI GKGCMC 3 cut(s) 17, 396, 618
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 119
BsuRI GGCC 3 cut(s) 15, 321, 493
BtsCI GGATG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 642
BveI ACCTGC 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 89, 418
Cfr10I RCCGGY 3 cut(s) 317, 493, 680
Cfr13I GGNCC 4 cut(s) 13, 14, 320, 491
Cfr9I CCCGGG 1 cut(s) 554
Csp6I GTAC 2 cut(s) 20, 521
CviAII CATG 3 cut(s) 109, 226, 586
CviQI GTAC 2 cut(s) 20, 521
DdeI CTNAG 1 cut(s) 346
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
EciI GGCGGA 2 cut(s) 20, 497
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 1 cut(s) 17
Eco88I CYCGRG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 3 cut(s) 112, 229, 589
FaiI YATR 4 cut(s) 110, 227, 587, 656
FaqI GGGAC 1 cut(s) 72
FatI CATG 3 cut(s) 108, 225, 585
FauI CCCGC 1 cut(s) 222
Fnu4HI GCNGC 4 cut(s) 274, 685, 688, 691
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 4 cut(s) 274, 685, 688, 691
FspBI CTAG 3 cut(s) 417, 437, 694
GluI GCNGC 4 cut(s) 274, 685, 688, 691
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 3 cut(s) 15, 321, 493
HapII CCGG 7 cut(s) 56, 77, 279, 318, 494, 555, 681
Hin1II CATG 3 cut(s) 112, 229, 589
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HinfI GANTC 2 cut(s) 60, 180
HpaI GTTAAC 1 cut(s) 528
HpaII CCGG 7 cut(s) 56, 77, 279, 318, 494, 555, 681
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 528, 616, 670
Hpy188I TCNGA 1 cut(s) 234
Hpy8I GTNNAC 3 cut(s) 528, 616, 670
HpyCH4III ACNGT 4 cut(s) 152, 424, 525, 646
HpyCH4V TGCA 4 cut(s) 160, 589, 616, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 279, 391, 547, 690
HpyF3I CTNAG 1 cut(s) 346
Hsp92II CATG 3 cut(s) 112, 229, 589
KspAI GTTAAC 1 cut(s) 528
Kzo9I GATC 1 cut(s) 172
LmnI GCTCC 3 cut(s) 54, 105, 361
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 1 cut(s) 112
MaeI CTAG 3 cut(s) 417, 437, 694
MaeIII GTNAC 1 cut(s) 424
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 2 cut(s) 58, 124
MhlI GDGCHC 3 cut(s) 17, 396, 618
MluCI AATT 1 cut(s) 262
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 4 cut(s) 74, 127, 257, 355
MseI TTAA 2 cut(s) 527, 579
MslI CAYNNNNRTG 1 cut(s) 611
MspI CCGG 7 cut(s) 56, 77, 279, 318, 494, 555, 681
MspR9I CCNGG 6 cut(s) 57, 78, 279, 534, 555, 556
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 4 cut(s) 279, 391, 547, 690
NciI CCSGG 5 cut(s) 57, 78, 279, 555, 556
NdeII GATC 1 cut(s) 172
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 3 cut(s) 112, 229, 589
NlaIV GGNNCC 4 cut(s) 14, 15, 322, 492
NmeAIII GCCGAG 1 cut(s) 590
NspI RCATGY 1 cut(s) 589
PfeI GAWTC 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 4 cut(s) 275, 686, 689, 692
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 4 cut(s) 14, 15, 322, 492
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 4 cut(s) 13, 14, 320, 491
RsaI GTAC 2 cut(s) 21, 522
RsaNI GTAC 2 cut(s) 20, 521
RseI CAYNNNNRTG 1 cut(s) 611
SaqAI TTAA 2 cut(s) 527, 579
SatI GCNGC 4 cut(s) 274, 685, 688, 691
Sau3AI GATC 1 cut(s) 172
Sau96I GGNCC 4 cut(s) 13, 14, 320, 491
ScaI AGTACT 1 cut(s) 21
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 6 cut(s) 57, 78, 279, 534, 555, 556
SduI GDGCHC 3 cut(s) 17, 396, 618
SetI ASST 6 cut(s) 96, 352, 387, 415, 552, 661
SfaNI GCATC 1 cut(s) 112
SgrAI CRCCGGYG 1 cut(s) 680
SmaI CCCGGG 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 611
SpeI ACTAGT 1 cut(s) 436
Sse9I AATT 1 cut(s) 262
SsiI CCGC 9 cut(s) 5, 215, 306, 359, 482, 506, 684, 687, 690
SspMI CTAG 3 cut(s) 417, 437, 694
StyD4I CCNGG 6 cut(s) 55, 76, 277, 532, 553, 554
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 4 cut(s) 152, 424, 525, 646
TaqI TCGA 1 cut(s) 96
TasI AATT 1 cut(s) 262
TatI WGTACW 1 cut(s) 19
TauI GCSGC 3 cut(s) 687, 690, 693
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 2 cut(s) 527, 579
Tru9I TTAA 2 cut(s) 527, 579
TscAI CASTG 1 cut(s) 649
TseI GCWGC 1 cut(s) 273
TspDTI ATGAA 2 cut(s) 125, 308
TspMI CCCGGG 1 cut(s) 554
TspRI CASTG 1 cut(s) 649
VneI GTGCAC 1 cut(s) 614
XceI RCATGY 1 cut(s) 589
XmaI CCCGGG 1 cut(s) 554
XspI CTAG 3 cut(s) 417, 437, 694
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.