RLG00000023573

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017297
Physical Location & Seq
Reverse (-)
25361850 .. 25363391
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023573

Sequence Viewer

Length: 984 bp
ATGGCTCTAGTAACTTTACCAACATCCATGACACTCCGTCCATTCGGCGGCAAGTTATTGCAGTACGTACATGAAGATGTGGAGGCACATGGGTTGCTCATAGCAGACGGAGACGACGTTATGAGTCCGTTAAGTAAGCTGGAGGTGTTGCAGGCACGGTCGGGTAACGGACGAGTGCATATAAGATGCAGTTATAACAACAAGTTCTTGGCCGTCCGGAGAGAAGGCGATTCCGTGGTGGCAGCGGTGGCCGAGGAACCTGTGGAGGACGAATCAGCATGGTCATGCACCATGTTCTTGCCTGCAAATATCCCTGGGTACGGTAATAACAACATTCAGTTGTTGCATGTGCAACTCGGACGCAACCTGTTCATCGATCGACAAAATCCTGTTCTTGCAGTATCATCAGAAGTAGCAGCAAATCCCCAACTTGCAACTCACATGATATTCGGTTTCGTCGATTTGGCACCCCTGTTCATGATGCCCAGACACGTGGCGTTTAAAGGGGAAAACGGCGATTACCTTCGGTTCATGTCGATCAACAACCAGCCATTTCTACAGTTCAGTTCCGGCGTCGACAAATGGCATCCATCGGTGTTGCACGAGGTCTCTAACAGCGATCCTGCTTCTGGAAGAGTTCGCATTAGGTGCCATGCTAGCAATGTCAACGACGGATTCTGGAGGCGTAACCGTGATTCTGATGCTGAATGGATTCATGCTGACTCAGTTCTCTTGCCGCACAACTCGCCCTTGGGCCACCGCTGGGACGACTCCCATTTTACGGCAGTCAAGGTTGCAGAAAATGATCAAGGCATCATCGTCGCTTTCAGAAGCGCCAACAACAACTACTTAACTCGACGGAGCGTTTTCAATGTGCAAAACGCTAGAGAGGAATCCCTACCATCCAGCGCAACCAAAGAAACAAAGACCTATCCAATTTCCCCTCATGCTTCTCGACTTCTGTCGTCTGTGCCTGCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

328

Amino Acids

36.29

Weight (kDa)

6.39

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 13 - 121 2.5e-15 Agglutinin domain
Agglutinin PF07468 164 - 242 1.5e-07 Agglutinin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017297)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03090 FvH4_6g05700 FvH4_6g22110
rosa_chinensis RchiOBHm_Chr3g0479441
rosa_laevigata RLG00000023573
rosa_multiflora Rmu_co8054592.1_g000001 Rmu_sc0003405.1_g000001 Rmu_sc0015035.1_g000004
rosa_roxburghii Rroxscaffold_6G00402270
rosa_rugosa Rorug03G0175300
rosa_samantha Rh3CG255500 Rh3DG251700
rosa_wichuraiana Rw3G020220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AccB1I GGYRCC 2 cut(s) 466, 648
AccI GTMKAC 1 cut(s) 576
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 4 cut(s) 48, 245, 737, 760
AclWI GGATC 1 cut(s) 614
AcoI YGGCCR 2 cut(s) 210, 249
AcvI CACGTG 1 cut(s) 493
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 3 cut(s) 65, 69, 320
AfiI CCNNNNNNNGG 5 cut(s) 47, 320, 629, 763, 781
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 1 cut(s) 871
AhdI GACNNNNNGTC 1 cut(s) 36
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 105, 613
AlwI GGATC 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 3 cut(s) 210, 249, 754
ApeKI GCWGC 2 cut(s) 242, 416
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 2 cut(s) 836, 911
AspS9I GGNCC 1 cut(s) 754
AsuNHI GCTAGC 1 cut(s) 656
BanI GGYRCC 2 cut(s) 466, 648
BauI CACGAG 1 cut(s) 602
BbrPI CACGTG 1 cut(s) 493
BbvI GCAGC 2 cut(s) 254, 428
BccI CCATC 2 cut(s) 598, 910
BceAI ACGGC 3 cut(s) 197, 529, 798
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 805
BcoDI GTCTC 2 cut(s) 105, 613
BfaI CTAG 4 cut(s) 8, 657, 885, 982
BfmI CTRYAG 1 cut(s) 557
BfoI RGCGCY 1 cut(s) 837
BisI GCNGC 4 cut(s) 49, 243, 417, 737
BlsI GCNGC 4 cut(s) 50, 244, 418, 738
Bme1390I CCNGG 1 cut(s) 315
BmeRI GACNNNNNGTC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 754
BmiI GGNNCC 3 cut(s) 258, 468, 650
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 5 cut(s) 176, 471, 595, 691, 822
BmtI GCTAGC 1 cut(s) 660
BoxI GACNNNNGTC 1 cut(s) 961
BpmI CTGGAG 2 cut(s) 161, 700
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 2 cut(s) 67, 493
BsaHI GRCGYC 1 cut(s) 573
BsaI GGTCTC 1 cut(s) 613
BsaJI CCNNGG 5 cut(s) 234, 252, 313, 314, 750
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 320, 629, 763, 781
Bse3DI GCAATG 1 cut(s) 667
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 1 cut(s) 315
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 5 cut(s) 234, 252, 313, 314, 750
BseGI GGATG 3 cut(s) 23, 586, 902
BseLI CCNNNNNNNGG 5 cut(s) 47, 320, 629, 763, 781
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 1 cut(s) 738
BseXI GCAGC 2 cut(s) 254, 428
BseYI CCCAGC 1 cut(s) 762
Bsh1285I CGRYCG 2 cut(s) 161, 379
BshFI GGCC 3 cut(s) 212, 251, 756
BshNI GGYRCC 2 cut(s) 466, 648
BshVI ATCGAT 1 cut(s) 375
BsiEI CGRYCG 2 cut(s) 161, 379
BsiSI CCGG 2 cut(s) 217, 570
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 5 cut(s) 47, 320, 629, 763, 781
BsmAI GTCTC 2 cut(s) 105, 613
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 779
BsnI GGCC 3 cut(s) 212, 251, 756
Bso31I GGTCTC 1 cut(s) 613
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 4 cut(s) 376, 537, 619, 805
BspACI CCGC 4 cut(s) 48, 245, 737, 760
BspANI GGCC 3 cut(s) 212, 251, 756
BspCNI CTCAG 1 cut(s) 737
BspDI ATCGAT 1 cut(s) 375
BspEI TCCGGA 1 cut(s) 216
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 258, 468, 650
BspOI GCTAGC 1 cut(s) 660
BspPI GGATC 1 cut(s) 614
BspT107I GGYRCC 2 cut(s) 466, 648
BspTNI GGTCTC 1 cut(s) 613
BsrDI GCAATG 1 cut(s) 667
BssECI CCNNGG 5 cut(s) 234, 252, 313, 314, 750
BssMI GATC 4 cut(s) 376, 537, 619, 805
BssNI GRCGYC 1 cut(s) 573
BssSI CACGAG 1 cut(s) 602
BssT1I CCWWGG 1 cut(s) 750
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 4 cut(s) 159, 323, 561, 692
Bst6I CTCTTC 1 cut(s) 628
BstACI GRCGYC 1 cut(s) 573
BstAPI GCANNNNNTGC 1 cut(s) 648
BstBAI YACGTR 2 cut(s) 67, 493
BstC8I GCNNGC 5 cut(s) 153, 303, 658, 975, 979
BstDEI CTNAG 1 cut(s) 724
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 3 cut(s) 23, 586, 902
BstH2I RGCGCY 1 cut(s) 837
BstHHI GCGC 2 cut(s) 836, 911
BstKTI GATC 4 cut(s) 379, 540, 622, 808
BstMAI GTCTC 2 cut(s) 105, 613
BstMBI GATC 4 cut(s) 376, 537, 619, 805
BstMCI CGRYCG 2 cut(s) 161, 379
BstMWI GCNNNNNNNGC 4 cut(s) 248, 648, 657, 745
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 350
BstPAI GACNNNNGTC 1 cut(s) 961
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 557
BstSNI TACGTA 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 254, 428
BstXI CCANNNNNNTGG 1 cut(s) 493
Bsu15I ATCGAT 1 cut(s) 375
BsuRI GGCC 3 cut(s) 212, 251, 756
BsuTUI ATCGAT 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 3 cut(s) 23, 586, 902
Cac8I GCNNGC 5 cut(s) 153, 303, 658, 975, 979
CciI TCATGA 1 cut(s) 477
CfoI GCGC 2 cut(s) 836, 911
Cfr13I GGNCC 1 cut(s) 754
ClaI ATCGAT 1 cut(s) 375
CseI GACGC 2 cut(s) 369, 562
Csp6I GTAC 3 cut(s) 64, 68, 319
CviJI RGCY 7 cut(s) 5, 139, 212, 251, 550, 756, 981
CviKI_1 RGCY 7 cut(s) 5, 139, 212, 251, 550, 756, 981
CviQI GTAC 3 cut(s) 64, 68, 319
DdeI CTNAG 1 cut(s) 724
DpnI GATC 4 cut(s) 378, 539, 621, 807
DpnII GATC 4 cut(s) 376, 537, 619, 805
DraI TTTAAA 1 cut(s) 502
DriI GACNNNNNGTC 1 cut(s) 36
EaeI YGGCCR 2 cut(s) 210, 249
Eam1104I CTCTTC 1 cut(s) 628
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 628
Eco105I TACGTA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 613
Eco72I CACGTG 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 750
Esp3I CGTCTC 1 cut(s) 105
FaqI GGGAC 1 cut(s) 779
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 49, 243, 417, 737
FokI GGATG 3 cut(s) 10, 573, 889
Fsp4HI GCNGC 4 cut(s) 49, 243, 417, 737
FspBI CTAG 4 cut(s) 8, 657, 885, 982
GlaI GCGC 2 cut(s) 835, 910
GluI GCNGC 4 cut(s) 49, 243, 417, 737
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 2 cut(s) 161, 700
HaeII RGCGCY 1 cut(s) 837
HaeIII GGCC 3 cut(s) 212, 251, 756
HapII CCGG 2 cut(s) 217, 570
HgaI GACGC 2 cut(s) 369, 562
HhaI GCGC 2 cut(s) 836, 911
Hin1I GRCGYC 1 cut(s) 573
Hin6I GCGC 2 cut(s) 834, 909
HinP1I GCGC 2 cut(s) 834, 909
HincII GTYRAC 2 cut(s) 577, 667
HindII GTYRAC 2 cut(s) 577, 667
HinfI GANTC 9 cut(s) 124, 230, 272, 675, 695, 712, 722, 770, 893
HpaII CCGG 2 cut(s) 217, 570
Hpy166II GTNNAC 2 cut(s) 577, 667
Hpy188I TCNGA 4 cut(s) 359, 409, 700, 830
Hpy188III TCNNGA 5 cut(s) 217, 478, 630, 679, 954
Hpy8I GTNNAC 2 cut(s) 577, 667
Hpy99I CGWCG 6 cut(s) 119, 461, 578, 674, 824, 861
HpyAV CCTTC 2 cut(s) 218, 533
HpyCH4III ACNGT 4 cut(s) 159, 323, 561, 692
HpyCH4IV ACGT 3 cut(s) 66, 117, 492
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 648, 657, 745
HpyF3I CTNAG 1 cut(s) 724
HpySE526I ACGT 3 cut(s) 66, 117, 492
Hsp92I GRCGYC 1 cut(s) 573
HspAI GCGC 2 cut(s) 834, 909
Kpn2I TCCGGA 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 4 cut(s) 376, 537, 619, 805
LmnI GCTCC 1 cut(s) 861
Lsp1109I GCAGC 2 cut(s) 254, 428
LweI GCATC 5 cut(s) 176, 471, 595, 691, 822
MaeI CTAG 4 cut(s) 8, 657, 885, 982
MaeII ACGT 3 cut(s) 66, 117, 492
MaeIII GTNAC 3 cut(s) 10, 164, 686
MalI GATC 4 cut(s) 378, 539, 621, 807
MboI GATC 4 cut(s) 376, 537, 619, 805
MboII GAAGA 2 cut(s) 86, 645
MluCI AATT 1 cut(s) 936
MlyI GAGTC 3 cut(s) 133, 716, 764
MnlI CCTC 8 cut(s) 76, 136, 247, 259, 598, 675, 883, 954
MroI TCCGGA 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 3 cut(s) 131, 501, 851
MslI CAYNNNNRTG 2 cut(s) 75, 283
MspA1I CMGCKG 2 cut(s) 245, 762
MspI CCGG 2 cut(s) 217, 570
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 4 cut(s) 248, 648, 657, 745
NdeII GATC 4 cut(s) 376, 537, 619, 805
NheI GCTAGC 1 cut(s) 656
NlaIV GGNNCC 3 cut(s) 258, 468, 650
NmeAIII GCCGAG 1 cut(s) 277
NspI RCATGY 1 cut(s) 350
PagI TCATGA 1 cut(s) 477
PasI CCCWGGG 1 cut(s) 314
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 456
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 6 cut(s) 230, 272, 675, 695, 712, 893
PkrI GCNGC 4 cut(s) 50, 244, 418, 738
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 3 cut(s) 132, 716, 764
PmaCI CACGTG 1 cut(s) 493
PmlI CACGTG 1 cut(s) 493
PpsI GAGTC 3 cut(s) 132, 716, 764
Ppu21I YACGTR 2 cut(s) 67, 493
PshAI GACNNNNGTC 1 cut(s) 961
PsiI TTATAA 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 313
PspCI CACGTG 1 cut(s) 493
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 258, 468, 650
PspPI GGNCC 1 cut(s) 754
PvuI CGATCG 1 cut(s) 379
RsaI GTAC 3 cut(s) 65, 69, 320
RsaNI GTAC 3 cut(s) 64, 68, 319
RseI CAYNNNNRTG 2 cut(s) 75, 283
SalI GTCGAC 1 cut(s) 575
SaqAI TTAA 3 cut(s) 131, 501, 851
SatI GCNGC 4 cut(s) 49, 243, 417, 737
Sau3AI GATC 4 cut(s) 376, 537, 619, 805
Sau96I GGNCC 1 cut(s) 754
SchI GAGTC 3 cut(s) 133, 716, 764
ScrFI CCNGG 1 cut(s) 315
SfaNI GCATC 5 cut(s) 176, 471, 595, 691, 822
SfcI CTRYAG 1 cut(s) 557
SmiMI CAYNNNNRTG 2 cut(s) 75, 283
SnaBI TACGTA 1 cut(s) 67
Sse9I AATT 1 cut(s) 936
SsiI CCGC 4 cut(s) 48, 245, 737, 760
SspMI CTAG 4 cut(s) 8, 657, 885, 982
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 4 cut(s) 159, 323, 561, 692
TaiI ACGT 3 cut(s) 69, 120, 495
TaqI TCGA 7 cut(s) 375, 379, 459, 536, 576, 856, 955
TasI AATT 1 cut(s) 936
TauI GCSGC 2 cut(s) 51, 739
TfiI GAWTC 6 cut(s) 230, 272, 675, 695, 712, 893
Tru1I TTAA 3 cut(s) 131, 501, 851
Tru9I TTAA 3 cut(s) 131, 501, 851
TseI GCWGC 2 cut(s) 242, 416
TspDTI ATGAA 5 cut(s) 87, 361, 466, 520, 704
TspGWI ACGGA 7 cut(s) 26, 117, 123, 183, 223, 687, 874
XceI RCATGY 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 4 cut(s) 8, 657, 885, 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.