RLG00000025232

Cyclic phosphodiesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011573
Physical Location & Seq
Reverse (-)
43215052 .. 43215800
749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025232

Sequence Viewer

Length: 513 bp
ATGGAGGCCATCCGGAAGGAATTTGGTGGGCCTGAGGTTGAGCCCCACATCACTGTTGCAGGGTCCATTCTCTTCAATCGCTCAGAGGCCATTGAAAAGTTCATCGGTGCATGTAATGAGATAAACCCCTATGTTTGTCAAGTCGATCAACTTGAGACTAGCAAATTCTATTTCCAATGTGTTTCCCTTGTCTTTCAGACAGATCATCAGTTGACACAGGAAACTGGACTCTTGGCTGCGCGTATGTATTGCAATAGTGGTAAGATCTTTGCAACAATTTTCAGGGAAAATCACATTACTATATCTAATATGCCACATTTGAGCTTGTTCTACGGGGATCTTACAGATGACGAGAAGAAAAGAGCAGTAGAAATAGTGAATAGTGTCGATGAGGGACTTTGTACCATTAGCTTCACCATAAATCGGATGCAATTGTACAGGGTTAACTTCGAAGATAAAACTCAAAAATCTTGGGAGATGGTTTCTGAATGCAACCTTCGACCTAAAATCTAA

Protein Analysis

171

Amino Acids

19.52

Weight (kDa)

5.25

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CPDase PF07823 11 - 159 4.2e-09 Cyclic phosphodiesterase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 241
AccIII TCCGGA 1 cut(s) 12
AclWI GGATC 1 cut(s) 345
AcsI RAATTY 2 cut(s) 20, 164
AfaI GTAC 2 cut(s) 403, 437
AfiI CCNNNNNNNGG 1 cut(s) 423
AgsI TTSAA 2 cut(s) 76, 95
AluBI AGCT 2 cut(s) 324, 411
AluI AGCT 2 cut(s) 324, 411
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 345
Aor13HI TCCGGA 1 cut(s) 12
AoxI GGCC 3 cut(s) 6, 29, 87
ApeKI GCWGC 1 cut(s) 236
ApoI RAATTY 2 cut(s) 20, 164
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 2 cut(s) 29, 63
AsuHPI GGTGA 1 cut(s) 406
AsuII TTCGAA 1 cut(s) 450
AvaII GGWCC 1 cut(s) 63
AxyI CCTNAGG 1 cut(s) 33
BanII GRGCYC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 223
BccI CCATC 2 cut(s) 17, 472
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 1 cut(s) 159
BglII AGATCT 1 cut(s) 264
BisI GCNGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 29, 63
BmiI GGNNCC 1 cut(s) 64
BmsI GCATC 1 cut(s) 417
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 173
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse1I ACTGG 1 cut(s) 229
Bse21I CCTNAGG 1 cut(s) 33
BseAI TCCGGA 1 cut(s) 12
BseGI GGATG 2 cut(s) 9, 432
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMII CTCAG 2 cut(s) 24, 96
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 241
BshFI GGCC 3 cut(s) 8, 31, 89
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 1 cut(s) 423
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 3 cut(s) 8, 31, 89
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 45
Bsp13I TCCGGA 1 cut(s) 12
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 4 cut(s) 145, 202, 264, 337
BspANI GGCC 3 cut(s) 8, 31, 89
BspCNI CTCAG 2 cut(s) 25, 95
BspEI TCCGGA 1 cut(s) 12
BspFNI CGCG 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 64
BspPI GGATC 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 1 cut(s) 229
BssMI GATC 4 cut(s) 145, 202, 264, 337
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 450
BstDEI CTNAG 2 cut(s) 33, 82
BstF5I GGATG 2 cut(s) 9, 432
BstFNI CGCG 1 cut(s) 241
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 4 cut(s) 148, 205, 267, 340
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 4 cut(s) 145, 202, 264, 337
BstNSI RCATGY 1 cut(s) 114
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 264, 337
BstYI RGATCY 2 cut(s) 264, 337
Bsu36I CCTNAGG 1 cut(s) 33
BsuRI GGCC 3 cut(s) 8, 31, 89
BtsCI GGATG 2 cut(s) 9, 432
BtsIMutI CAGTG 1 cut(s) 51
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 2 cut(s) 29, 63
Csp6I GTAC 2 cut(s) 402, 436
CviAII CATG 1 cut(s) 111
CviJI RGCY 7 cut(s) 8, 31, 43, 89, 236, 324, 411
CviKI_1 RGCY 7 cut(s) 8, 31, 43, 89, 236, 324, 411
CviQI GTAC 2 cut(s) 402, 436
DdeI CTNAG 2 cut(s) 33, 82
DpnI GATC 4 cut(s) 147, 204, 266, 339
DpnII GATC 4 cut(s) 145, 202, 264, 337
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 63
Eco81I CCTNAGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 45
FaeI CATG 1 cut(s) 114
FaiI YATR 6 cut(s) 112, 132, 245, 302, 311, 419
FaqI GGGAC 1 cut(s) 408
FatI CATG 1 cut(s) 110
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 1 cut(s) 439
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 1 cut(s) 237
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 237
HaeIII GGCC 3 cut(s) 8, 31, 89
HapII CCGG 1 cut(s) 13
HhaI GCGC 1 cut(s) 241
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 2 cut(s) 213, 445
HindII GTYRAC 2 cut(s) 213, 445
HinfI GANTC 1 cut(s) 228
HpaI GTTAAC 1 cut(s) 445
HpaII CCGG 1 cut(s) 13
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 2 cut(s) 213, 445
Hpy188I TCNGA 4 cut(s) 85, 198, 426, 487
Hpy188III TCNNGA 1 cut(s) 13
Hpy8I GTNNAC 2 cut(s) 213, 445
HpyAV CCTTC 2 cut(s) 10, 506
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 6 cut(s) 59, 110, 252, 272, 430, 492
HpyF3I CTNAG 2 cut(s) 33, 82
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 1 cut(s) 239
Kpn2I TCCGGA 1 cut(s) 12
KspAI GTTAAC 1 cut(s) 445
Kzo9I GATC 4 cut(s) 145, 202, 264, 337
LpnPI CCDG 7 cut(s) 26, 45, 45, 203, 210, 268, 424
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 417
MaeI CTAG 1 cut(s) 159
MalI GATC 4 cut(s) 147, 204, 266, 339
MboI GATC 4 cut(s) 145, 202, 264, 337
MboII GAAGA 3 cut(s) 64, 367, 464
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 2 cut(s) 264, 337
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 4 cut(s) 20, 164, 276, 431
MlyI GAGTC 1 cut(s) 222
MnlI CCTC 3 cut(s) 28, 79, 385
MroI TCCGGA 1 cut(s) 12
MseI TTAA 1 cut(s) 444
MspI CCGG 1 cut(s) 13
MunI CAATTG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 494
MvnI CGCG 1 cut(s) 241
NdeII GATC 4 cut(s) 145, 202, 264, 337
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 1 cut(s) 64
NspI RCATGY 1 cut(s) 114
NspV TTCGAA 1 cut(s) 450
PctI GAATGC 1 cut(s) 494
PkrI GCNGC 1 cut(s) 238
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 2 cut(s) 29, 63
PsuI RGATCY 2 cut(s) 264, 337
RsaI GTAC 2 cut(s) 403, 437
RsaNI GTAC 2 cut(s) 402, 436
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 4 cut(s) 145, 202, 264, 337
Sau96I GGNCC 2 cut(s) 29, 63
SchI GAGTC 1 cut(s) 222
SduI GDGCHC 1 cut(s) 45
SetI ASST 5 cut(s) 39, 326, 413, 498, 505
SfaNI GCATC 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 4 cut(s) 20, 164, 276, 431
SspMI CTAG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 4 cut(s) 144, 387, 450, 499
TasI AATT 4 cut(s) 20, 164, 276, 431
TatI WGTACW 1 cut(s) 435
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 1 cut(s) 58
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 1 cut(s) 58
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 2 cut(s) 20, 164
XceI RCATGY 1 cut(s) 114
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.