RLG00000025777

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0019909
Physical Location & Seq
Reverse (-)
48216667 .. 48218692
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025777

Sequence Viewer

Length: 1332 bp
ATGGGGTTCTTCATGCTTTCCAACGAGAATTTCTTGAATGAGGATTGGGATCTTATAGATTTTGAAAAGCTTGCTAAAGGGGGTGACTTACCCATCGAAGAACCAGTGGATGGTGACATCGACATCACTCTTGACTTCGGAGATATGTGTCCATTTACGAGCACTGGAGCTAAATTTAAGGGCACTGGAACTAATTTTAAGTTCTATGGAAATCATGAGGCTACCATTAAGATGTACACAAGTGACACCCTCAGGAAGTTGAGAGAAGAGATACTAAGGCACTTTGATGAACATTTGGGGATCGAATTACGTTTTGGTGAGGAAGTAATATTGAAGTTAGACGGCGATACTCTGTCCTTTCGGGATTGTGGCATTTACGGGACTCAAGATATCAATGTTATCTTGAGATCACCACCAATCTATGATCTCGGTGACTTGACTTCTGTTGGGGAGATAGATGTGATAATACATGCAAAAGGATTTCCATGGTTCATTGTTCCAGTGTTTTTGCTGGATTCTGTGCAAGACATCAAGGAAAAGATTATGATGATCACATTCATTGACCGGGAGAACCAAATGCTGTATTACAGAAAGAAGGTGCTTGAAGAGGATAGCATACTTGCCAACTGTGGACTGTACAACAACTGCCGCATTCAGTTAGTTAATACTGGCAAACCCCGCGAACCCTCTGCATCTCAGCCTGAATCAACACCTGTATCTGTGCCTGAATCAACACCTGGAGCTACTATAAGTAATGAGATCATTCGTGTCTGCCTCTATTACGGGGACTTCAACCTGCCTCTTCCCTCTGCTACCTTTGAAGTGAAGGCAGATAGCTGCCTAAACGTATTGAAAGCAGAGATATTGAAGCAGGTTGAGAGCTACTTGGTCCTTAAATTACCCTCTGCTGAAGAAGTAAGAGTAGGTGGATCTAGTCAGTGTGATATATCGCTTGCCCAGTGTCAGCTTTTTGGAATGGCAAATATCAAGGTGCTCTTGAAACAACCACCACCAGAATGGCTTGGCCCTTATTGTGGAGGAGTGCTCTCTACTAGGTCTATGGCTTTGAAAGAATTGGTAATTTTACGAAATGGAACAGAGAGATGGCCAGCAGTAAGGTTCAAGCTTCGCATGCATCCTCAGACAAAGGTTCTACTTATAAAGGAAGTGATTGAGAAGATTTCCAATGCTAAAAAGTGCTATCAGAGGCTGCTGAACTATGCAACTGAAGAAGAGTTGTTTGATTATTCCCAGATTTCCCAGATCTGTACAATGATAGAGCTCAGAATTAGTGTTCAAGGTTTATCAGAGGCTGCTGAACTATGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

50.04

Weight (kDa)

4.96

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 165 - 221 4.1e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019909)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0449871
rosa_laevigata RLG00000025777
rosa_samantha Rh3AG022700 Rh3BG023400 Rh3CG022400 Rh3DG023300
rosa_wichuraiana Rw3G001830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1160
Acc36I ACCTGC 2 cut(s) 804, 862
AccB7I CCANNNNNTGG 1 cut(s) 110
AccII CGCG 1 cut(s) 681
AciI CCGC 2 cut(s) 649, 679
AclWI GGATC 3 cut(s) 57, 308, 937
AcoI YGGCCR 1 cut(s) 1106
AcsI RAATTY 2 cut(s) 28, 173
AcuI CTGAAG 2 cut(s) 930, 1248
AfaI GTAC 3 cut(s) 236, 638, 1270
AfiI CCNNNNNNNGG 2 cut(s) 110, 1034
AjnI CCWGG 1 cut(s) 736
AjuI GAANNNNNNNTTGG 4 cut(s) 14, 46, 297, 329
AluBI AGCT 8 cut(s) 70, 170, 743, 837, 882, 967, 1126, 1282
AluI AGCT 8 cut(s) 70, 170, 743, 837, 882, 967, 1126, 1282
Alw21I GWGCWC 4 cut(s) 164, 996, 1047, 1284
AlwI GGATC 3 cut(s) 57, 308, 937
AlwNI CAGNNNCTG 2 cut(s) 1210, 1313
AoxI GGCC 2 cut(s) 1024, 1106
ApeKI GCWGC 3 cut(s) 837, 1210, 1313
ApoI RAATTY 2 cut(s) 28, 173
AspS9I GGNCC 2 cut(s) 889, 1025
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 5 cut(s) 95, 125, 329, 402, 443
AvaII GGWCC 1 cut(s) 889
AxyI CCTNAGG 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 185
BaeI ACNNNNGTAYC 2 cut(s) 699, 732
BalI TGGCCA 1 cut(s) 1108
BanII GRGCYC 1 cut(s) 1284
Bbv12I GWGCWC 4 cut(s) 164, 996, 1047, 1284
BbvI GCAGC 3 cut(s) 824, 1197, 1300
BccI CCATC 3 cut(s) 101, 104, 1098
BceAI ACGGC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 1 cut(s) 738
BclI TGATCA 1 cut(s) 549
BcnI CCSGG 1 cut(s) 566
BfaI CTAG 2 cut(s) 933, 1053
BfuAI ACCTGC 2 cut(s) 804, 862
BglII AGATCT 1 cut(s) 1263
BisI GCNGC 4 cut(s) 649, 838, 1211, 1314
BlsI GCNGC 4 cut(s) 650, 839, 1212, 1315
Bme1390I CCNGG 2 cut(s) 566, 738
Bme18I GGWCC 1 cut(s) 889
BmgT120I GGNCC 2 cut(s) 889, 1025
BmrFI CCNGG 2 cut(s) 566, 738
BmrI ACTGGG 1 cut(s) 952
BmsI GCATC 2 cut(s) 701, 1144
BmuI ACTGGG 1 cut(s) 952
BplI GAGNNNNNCTC 2 cut(s) 1029, 1061
BpmI CTGGAG 2 cut(s) 186, 759
BpuEI CTTGAG 2 cut(s) 369, 424
BpuMI CCSGG 1 cut(s) 566
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 485
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 1034
Bse1I ACTGG 6 cut(s) 104, 169, 190, 500, 673, 958
Bse21I CCTNAGG 1 cut(s) 251
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 115, 1135
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 110, 1034
BseMII CTCAG 4 cut(s) 265, 710, 1154, 1297
BseNI ACTGG 6 cut(s) 104, 169, 190, 500, 673, 958
BseRI GAGGAG 1 cut(s) 1053
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 3 cut(s) 824, 1197, 1300
Bsh1236I CGCG 1 cut(s) 681
BshFI GGCC 2 cut(s) 1026, 1108
BsiHKAI GWGCWC 4 cut(s) 164, 996, 1047, 1284
BsiSI CCGG 1 cut(s) 565
BslFI GGGAC 2 cut(s) 394, 800
BslI CCNNNNNNNGG 2 cut(s) 110, 1034
BsmFI GGGAC 2 cut(s) 394, 800
BsmI GAATGC 1 cut(s) 651
BsnI GGCC 2 cut(s) 1026, 1108
Bsp1286I GDGCHC 5 cut(s) 164, 185, 996, 1047, 1284
Bsp1407I TGTACA 3 cut(s) 234, 636, 1268
Bsp143I GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 2 cut(s) 649, 679
BspANI GGCC 2 cut(s) 1026, 1108
BspCNI CTCAG 4 cut(s) 264, 709, 1153, 1296
BspFNI CGCG 1 cut(s) 681
BspHI TCATGA 1 cut(s) 214
BspMI ACCTGC 2 cut(s) 804, 862
BspPI GGATC 3 cut(s) 57, 308, 937
BsrGI TGTACA 3 cut(s) 234, 636, 1268
BsrI ACTGG 6 cut(s) 104, 169, 190, 500, 673, 958
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
BssT1I CCWWGG 1 cut(s) 485
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 2 cut(s) 629, 636
Bst6I CTCTTC 4 cut(s) 261, 600, 807, 1227
BstAUI TGTACA 3 cut(s) 234, 636, 1268
BstC8I GCNNGC 4 cut(s) 72, 954, 1110, 1133
BstDEI CTNAG 5 cut(s) 251, 275, 696, 1140, 1283
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 2 cut(s) 115, 1135
BstFNI CGCG 1 cut(s) 681
BstKTI GATC 8 cut(s) 52, 303, 410, 427, 552, 762, 932, 1266
BstMBI GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
BstMWI GCNNNNNNNGC 2 cut(s) 678, 1132
BstNI CCWGG 1 cut(s) 738
BstNSI RCATGY 2 cut(s) 473, 1135
BstSCI CCNGG 2 cut(s) 564, 736
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 3 cut(s) 824, 1197, 1300
BstX2I RGATCY 3 cut(s) 49, 929, 1263
BstXI CCANNNNNNTGG 1 cut(s) 1017
BstYI RGATCY 3 cut(s) 49, 929, 1263
Bsu36I CCTNAGG 1 cut(s) 251
BsuRI GGCC 2 cut(s) 1026, 1108
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 2 cut(s) 115, 1135
BtsIMutI CAGTG 7 cut(s) 111, 162, 183, 507, 944, 965, 1327
BveI ACCTGC 2 cut(s) 804, 862
Cac8I GCNNGC 4 cut(s) 72, 954, 1110, 1133
CaiI CAGNNNCTG 2 cut(s) 1210, 1313
CciI TCATGA 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 889, 1025
Csp6I GTAC 3 cut(s) 235, 637, 1269
CviAII CATG 5 cut(s) 13, 215, 470, 486, 1132
CviQI GTAC 3 cut(s) 235, 637, 1269
DdeI CTNAG 5 cut(s) 251, 275, 696, 1140, 1283
DpnI GATC 8 cut(s) 51, 302, 409, 426, 551, 761, 931, 1265
DpnII GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
EaeI YGGCCR 1 cut(s) 1106
Eam1104I CTCTTC 4 cut(s) 261, 600, 807, 1227
EarI CTCTTC 4 cut(s) 261, 600, 807, 1227
Ecl136II GAGCTC 1 cut(s) 1282
Eco130I CCWWGG 1 cut(s) 485
Eco24I GRGCYC 1 cut(s) 1284
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 1282
Eco57I CTGAAG 2 cut(s) 930, 1248
Eco81I CCTNAGG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 1282
EcoRII CCWGG 1 cut(s) 736
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 485
EcoT22I ATGCAT 1 cut(s) 1137
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 5 cut(s) 16, 218, 473, 489, 1135
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FaqI GGGAC 2 cut(s) 394, 800
FatI CATG 5 cut(s) 12, 214, 469, 485, 1131
FauI CCCGC 1 cut(s) 686
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 4 cut(s) 649, 838, 1211, 1314
FokI GGATG 2 cut(s) 122, 1122
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 4 cut(s) 649, 838, 1211, 1314
FspBI CTAG 2 cut(s) 933, 1053
GluI GCNGC 4 cut(s) 649, 838, 1211, 1314
GsuI CTGGAG 2 cut(s) 186, 759
HaeIII GGCC 2 cut(s) 1026, 1108
HapII CCGG 1 cut(s) 565
Hin1II CATG 5 cut(s) 16, 218, 473, 489, 1135
HindIII AAGCTT 2 cut(s) 68, 1124
HinfI GANTC 4 cut(s) 382, 515, 704, 728
HpaII CCGG 1 cut(s) 565
HphI GGTGA 5 cut(s) 95, 125, 329, 402, 443
Hpy166II GTNNAC 2 cut(s) 237, 632
Hpy188I TCNGA 5 cut(s) 140, 1143, 1206, 1286, 1309
Hpy188III TCNNGA 8 cut(s) 34, 131, 215, 253, 362, 386, 403, 997
Hpy8I GTNNAC 2 cut(s) 237, 632
HpyAV CCTTC 2 cut(s) 589, 820
HpyCH4III ACNGT 2 cut(s) 629, 636
HpyCH4IV ACGT 2 cut(s) 310, 846
HpyCH4V TGCA 5 cut(s) 473, 523, 692, 1135, 1223
HpyF10VI GCNNNNNNNGC 2 cut(s) 678, 1132
HpyF3I CTNAG 5 cut(s) 251, 275, 696, 1140, 1283
HpySE526I ACGT 2 cut(s) 310, 846
Hsp92II CATG 5 cut(s) 16, 218, 473, 489, 1135
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
LmnI GCTCC 2 cut(s) 167, 740
Lsp1109I GCAGC 3 cut(s) 824, 1197, 1300
LweI GCATC 2 cut(s) 701, 1144
MaeI CTAG 2 cut(s) 933, 1053
MaeII ACGT 2 cut(s) 310, 846
MaeIII GTNAC 4 cut(s) 83, 113, 242, 431
MalI GATC 8 cut(s) 51, 302, 409, 426, 551, 761, 931, 1265
MboI GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
MboII GAAGA 8 cut(s) 110, 278, 617, 794, 923, 1189, 1241, 1244
MflI RGATCY 3 cut(s) 49, 929, 1263
MhlI GDGCHC 5 cut(s) 164, 185, 996, 1047, 1284
MlsI TGGCCA 1 cut(s) 1108
MluCI AATT 8 cut(s) 28, 173, 193, 305, 896, 1073, 1080, 1287
MluNI TGGCCA 1 cut(s) 1108
MlyI GAGTC 1 cut(s) 376
MmeI TCCRAC 1 cut(s) 45
Mox20I TGGCCA 1 cut(s) 1108
Mph1103I ATGCAT 1 cut(s) 1137
MscI TGGCCA 1 cut(s) 1108
MseI TTAA 5 cut(s) 177, 198, 228, 663, 894
MslI CAYNNNNRTG 3 cut(s) 230, 285, 1015
Msp20I TGGCCA 1 cut(s) 1108
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 2 cut(s) 566, 738
Mva1269I GAATGC 1 cut(s) 651
MvaI CCWGG 1 cut(s) 738
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 2 cut(s) 678, 1132
NciI CCSGG 1 cut(s) 566
NcoI CCATGG 1 cut(s) 485
NdeII GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
NlaIII CATG 5 cut(s) 16, 218, 473, 489, 1135
NmuCI GTSAC 4 cut(s) 83, 113, 242, 431
NsiI ATGCAT 1 cut(s) 1137
NspI RCATGY 2 cut(s) 473, 1135
PaeI GCATGC 1 cut(s) 1135
PagI TCATGA 1 cut(s) 214
PctI GAATGC 1 cut(s) 651
PfeI GAWTC 3 cut(s) 515, 704, 728
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 4 cut(s) 650, 839, 1212, 1315
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PsiI TTATAA 1 cut(s) 1160
Psp124BI GAGCTC 1 cut(s) 1284
Psp6I CCWGG 1 cut(s) 736
PspGI CCWGG 1 cut(s) 736
PspPI GGNCC 2 cut(s) 889, 1025
PstNI CAGNNNCTG 2 cut(s) 1210, 1313
PsuI RGATCY 3 cut(s) 49, 929, 1263
RsaI GTAC 3 cut(s) 236, 638, 1270
RsaNI GTAC 3 cut(s) 235, 637, 1269
RseI CAYNNNNRTG 3 cut(s) 230, 285, 1015
SacI GAGCTC 1 cut(s) 1284
SaqAI TTAA 5 cut(s) 177, 198, 228, 663, 894
SatI GCNGC 4 cut(s) 649, 838, 1211, 1314
Sau3AI GATC 8 cut(s) 49, 300, 407, 424, 549, 759, 929, 1263
Sau96I GGNCC 2 cut(s) 889, 1025
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 2 cut(s) 566, 738
SduI GDGCHC 5 cut(s) 164, 185, 996, 1047, 1284
SfaNI GCATC 2 cut(s) 701, 1144
SinI GGWCC 1 cut(s) 889
SmiMI CAYNNNNRTG 3 cut(s) 230, 285, 1015
SmlI CTYRAG 2 cut(s) 384, 403
SmoI CTYRAG 2 cut(s) 384, 403
SphI GCATGC 1 cut(s) 1135
Sse9I AATT 8 cut(s) 28, 173, 193, 305, 896, 1073, 1080, 1287
SsiI CCGC 2 cut(s) 649, 679
SspI AATATT 1 cut(s) 330
SspMI CTAG 2 cut(s) 933, 1053
SstI GAGCTC 1 cut(s) 1284
StyD4I CCNGG 2 cut(s) 564, 736
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 2 cut(s) 629, 636
TaiI ACGT 2 cut(s) 313, 849
TaqI TCGA 3 cut(s) 96, 120, 303
TasI AATT 8 cut(s) 28, 173, 193, 305, 896, 1073, 1080, 1287
TatI WGTACW 3 cut(s) 234, 636, 1268
TauI GCSGC 1 cut(s) 651
TfiI GAWTC 3 cut(s) 515, 704, 728
Tru1I TTAA 5 cut(s) 177, 198, 228, 663, 894
Tru9I TTAA 5 cut(s) 177, 198, 228, 663, 894
TscAI CASTG 6 cut(s) 111, 169, 190, 507, 944, 965
TseFI GTSAC 4 cut(s) 83, 113, 242, 431
TseI GCWGC 3 cut(s) 837, 1210, 1313
Tsp45I GTSAC 4 cut(s) 83, 113, 242, 431
TspDTI ATGAA 3 cut(s) 303, 481, 547
TspRI CASTG 6 cut(s) 111, 169, 190, 507, 944, 965
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 889
XapI RAATTY 2 cut(s) 28, 173
XceI RCATGY 2 cut(s) 473, 1135
XcmI CCANNNNNNNNNTGG 1 cut(s) 1014
XspI CTAG 2 cut(s) 933, 1053
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.