Rh3DG023300

Ubiquitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0019909
Physical Location & Seq
Forward (+)
1526651 .. 1528863
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG023300.1

Sequence Viewer

Length: 1116 bp
ATGAACATTTGGGGATCGAAAGAAGTGATATTGAAGTTAGACGGCGATACTCTGTCCTTTCGGGATTGTGACATTTCCAGGACTCAAGATATCAATGTTATCTTGAGATCACCACCACCAATCTATGATCTCGGTGACTTGACTTCTGTTGGGGAGATAGATGTGATAATACATGCAAAAGGATTTCCAAGGTTCATTGTTCCAGTGTTTTTGCTGGATTCTGTGCAAGACATCAAGGAAAAGATTATGATGATCACATTCATTGACCAGGAGAACCAAATGCTGTATTACAGAAAGAAGGTGCTTGAAGAGGATAGCATACTTGCCTACTGTGGACTGTACAACAACTGCCGCATTCAGTTAGTTAATACTGGCAAACCCCGCAAACCCTGCAAACCCTCTGCATCATCTGAGCCTGAATCATCAACACCTGCATCTGAGCCTGAATCAACACCTGCATCTGAGCCTGAATCAACACCTGTATCTGTGCCTGAATCAACACCTGGAGCTACTATACGTAATGATACTATACGTAATGAGATCATTCGTGTCTGCCTCTATTACGGGGACTTCAACCTGCCTCTTCCCTCTGCTACCTTTGAAGTGAAGGCGGATAGCTGCCTCAACGTATTGAAAGCAGAGATATTGAAGCAGGTTGAGAGCTACTTGGTCCTTAAATTGCCCTCTGCTGAAGAAGTAAGAGTAGGTGGATCTAGTCAGTGTGATATATCTCTTGCCCAGTGTCAGCTTTTTGGAATGGCAAATATCAAGGTGCTCTTGAAACAACCACCACCAGAATGGCTTGGCCCTTATTGTGGAGGAGTGCTCTCTACTAGGTCTATGATGACTTTGAAAGAATTGGTAATTTTACGAAATGGAACAGATAGATGGCCAGCAGTAAGGTTCAAGCTTCGTATGCATCCTCAGACAATGGTTCTACATATAAAGGAAGTGATTGAGAAGATTTCCAATGCTAAAAAGCGCTATCAGAGGCTGCTGAACTATGCAACTGAAGAAGAATTGTTTGATTATTCCCAGATTTCCCAGATCTGTACCATGATAGAGCTCAGAATTAGTGTTCAAGGTTTATCAGAGGCTGCTAAACTATGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

371

Amino Acids

41.73

Weight (kDa)

5.78

Isoelectric Point (pI)

58.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 65 - 122 2.7e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019909)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0449871
rosa_laevigata RLG00000025777
rosa_samantha Rh3AG022700 Rh3BG023400 Rh3CG022400 Rh3DG023300
rosa_wichuraiana Rw3G001830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 439, 463
Acc36I ACCTGC 4 cut(s) 439, 463, 585, 643
AciI CCGC 3 cut(s) 352, 382, 611
AclWI GGATC 2 cut(s) 22, 718
AcoI YGGCCR 1 cut(s) 890
AcuI CTGAAG 2 cut(s) 711, 1032
AfaI GTAC 2 cut(s) 341, 1054
AfeI AGCGCT 1 cut(s) 983
AfiI CCNNNNNNNGG 1 cut(s) 815
AjnI CCWGG 3 cut(s) 77, 267, 502
AluBI AGCT 6 cut(s) 509, 618, 663, 748, 910, 1066
AluI AGCT 6 cut(s) 509, 618, 663, 748, 910, 1066
Alw21I GWGCWC 3 cut(s) 777, 828, 1068
AlwI GGATC 2 cut(s) 22, 718
AlwNI CAGNNNCTG 2 cut(s) 994, 1097
Aor51HI AGCGCT 1 cut(s) 983
AoxI GGCC 2 cut(s) 805, 890
ApeKI GCWGC 3 cut(s) 618, 994, 1097
AspLEI GCGC 1 cut(s) 984
AspS9I GGNCC 2 cut(s) 670, 806
AsuHPI GGTGA 2 cut(s) 102, 146
AvaII GGWCC 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BalI TGGCCA 1 cut(s) 892
BanII GRGCYC 1 cut(s) 1068
Bbv12I GWGCWC 3 cut(s) 777, 828, 1068
BbvI GCAGC 3 cut(s) 605, 981, 1084
BccI CCATC 1 cut(s) 882
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 3 cut(s) 79, 269, 504
BclI TGATCA 1 cut(s) 252
BfaI CTAG 2 cut(s) 714, 834
BfoI RGCGCY 1 cut(s) 985
BfuAI ACCTGC 4 cut(s) 439, 463, 585, 643
BglII AGATCT 1 cut(s) 1047
BisI GCNGC 4 cut(s) 352, 619, 995, 1098
BlsI GCNGC 4 cut(s) 353, 620, 996, 1099
Bme1390I CCNGG 3 cut(s) 79, 269, 504
Bme18I GGWCC 1 cut(s) 670
BmgT120I GGNCC 2 cut(s) 670, 806
BmrFI CCNGG 3 cut(s) 79, 269, 504
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 4 cut(s) 413, 443, 467, 928
BmuI ACTGGG 1 cut(s) 733
BplI GAGNNNNNCTC 2 cut(s) 810, 842
BpmI CTGGAG 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 69, 124
BsaAI YACGTR 2 cut(s) 518, 533
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 815
Bse1I ACTGG 3 cut(s) 203, 376, 739
BseBI CCWGG 3 cut(s) 79, 269, 504
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 1 cut(s) 919
BseLI CCNNNNNNNGG 1 cut(s) 815
BseMII CTCAG 5 cut(s) 402, 429, 453, 938, 1081
BseNI ACTGG 3 cut(s) 203, 376, 739
BseRI GAGGAG 1 cut(s) 834
BseXI GCAGC 3 cut(s) 605, 981, 1084
BshFI GGCC 2 cut(s) 807, 892
BsiHKAI GWGCWC 3 cut(s) 777, 828, 1068
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 1 cut(s) 815
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 2 cut(s) 807, 892
Bsp1286I GDGCHC 3 cut(s) 777, 828, 1068
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
BspACI CCGC 3 cut(s) 352, 382, 611
BspANI GGCC 2 cut(s) 807, 892
BspCNI CTCAG 5 cut(s) 403, 430, 454, 937, 1080
BspMI ACCTGC 4 cut(s) 439, 463, 585, 643
BspPI GGATC 2 cut(s) 22, 718
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 3 cut(s) 203, 376, 739
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 79, 269, 504
Bst4CI ACNGT 2 cut(s) 332, 339
Bst6I CTCTTC 2 cut(s) 303, 588
BstAPI GCANNNNNTGC 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 2 cut(s) 518, 533
BstC8I GCNNGC 1 cut(s) 894
BstDEI CTNAG 5 cut(s) 411, 438, 462, 924, 1067
BstF5I GGATG 1 cut(s) 919
BstH2I RGCGCY 1 cut(s) 985
BstHHI GCGC 1 cut(s) 984
BstKTI GATC 7 cut(s) 17, 110, 130, 255, 543, 713, 1050
BstMBI GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
BstMWI GCNNNNNNNGC 3 cut(s) 381, 390, 916
BstNI CCWGG 3 cut(s) 79, 269, 504
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 3 cut(s) 77, 267, 502
BstSNI TACGTA 2 cut(s) 518, 533
BstV1I GCAGC 3 cut(s) 605, 981, 1084
BstX2I RGATCY 2 cut(s) 710, 1047
BstXI CCANNNNNNTGG 1 cut(s) 798
BstYI RGATCY 2 cut(s) 710, 1047
BsuRI GGCC 2 cut(s) 807, 892
BtsCI GGATG 1 cut(s) 919
BtsIMutI CAGTG 4 cut(s) 210, 725, 746, 1111
BveI ACCTGC 4 cut(s) 439, 463, 585, 643
Cac8I GCNNGC 1 cut(s) 894
CaiI CAGNNNCTG 2 cut(s) 994, 1097
CfoI GCGC 1 cut(s) 984
Cfr13I GGNCC 2 cut(s) 670, 806
Csp6I GTAC 2 cut(s) 340, 1053
CviAII CATG 2 cut(s) 173, 1057
CviQI GTAC 2 cut(s) 340, 1053
DdeI CTNAG 5 cut(s) 411, 438, 462, 924, 1067
DpnI GATC 7 cut(s) 16, 109, 129, 254, 542, 712, 1049
DpnII GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 2 cut(s) 303, 588
EarI CTCTTC 2 cut(s) 303, 588
EciI GGCGGA 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 1066
Eco105I TACGTA 2 cut(s) 518, 533
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 1068
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 670
Eco47III AGCGCT 1 cut(s) 983
Eco53kI GAGCTC 1 cut(s) 1066
Eco57I CTGAAG 2 cut(s) 711, 1032
EcoICRI GAGCTC 1 cut(s) 1066
EcoRII CCWGG 3 cut(s) 77, 267, 502
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 1 cut(s) 1068
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 2 cut(s) 176, 1060
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 581
FatI CATG 2 cut(s) 172, 1056
FauI CCCGC 1 cut(s) 389
FbaI TGATCA 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 352, 619, 995, 1098
FokI GGATG 1 cut(s) 906
FriOI GRGCYC 1 cut(s) 1068
Fsp4HI GCNGC 4 cut(s) 352, 619, 995, 1098
FspBI CTAG 2 cut(s) 714, 834
GlaI GCGC 1 cut(s) 983
GluI GCNGC 4 cut(s) 352, 619, 995, 1098
GsuI CTGGAG 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 985
HaeIII GGCC 2 cut(s) 807, 892
HhaI GCGC 1 cut(s) 984
Hin1II CATG 2 cut(s) 176, 1060
Hin6I GCGC 1 cut(s) 982
HinP1I GCGC 1 cut(s) 982
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 6 cut(s) 82, 218, 419, 446, 470, 494
HphI GGTGA 2 cut(s) 102, 146
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 7 cut(s) 412, 439, 463, 927, 990, 1070, 1093
Hpy188III TCNNGA 4 cut(s) 62, 86, 103, 778
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 2 cut(s) 292, 601
HpyCH4III ACNGT 2 cut(s) 332, 339
HpyCH4IV ACGT 3 cut(s) 517, 532, 627
HpyCH4V TGCA 8 cut(s) 176, 226, 393, 404, 434, 458, 919, 1007
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 390, 916
HpyF3I CTNAG 5 cut(s) 411, 438, 462, 924, 1067
HpySE526I ACGT 3 cut(s) 517, 532, 627
Hsp92II CATG 2 cut(s) 176, 1060
HspAI GCGC 1 cut(s) 982
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 3 cut(s) 605, 981, 1084
LweI GCATC 4 cut(s) 413, 443, 467, 928
MaeI CTAG 2 cut(s) 714, 834
MaeII ACGT 3 cut(s) 517, 532, 627
MaeIII GTNAC 2 cut(s) 68, 134
MalI GATC 7 cut(s) 16, 109, 129, 254, 542, 712, 1049
MboI GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
MboII GAAGA 6 cut(s) 320, 575, 704, 973, 1025, 1028
MflI RGATCY 2 cut(s) 710, 1047
MhlI GDGCHC 3 cut(s) 777, 828, 1068
MlsI TGGCCA 1 cut(s) 892
MluCI AATT 5 cut(s) 677, 857, 864, 1019, 1071
MluNI TGGCCA 1 cut(s) 892
MlyI GAGTC 1 cut(s) 76
Mox20I TGGCCA 1 cut(s) 892
Mph1103I ATGCAT 1 cut(s) 921
MscI TGGCCA 1 cut(s) 892
MseI TTAA 2 cut(s) 366, 675
MslI CAYNNNNRTG 1 cut(s) 796
Msp20I TGGCCA 1 cut(s) 892
MspR9I CCNGG 3 cut(s) 79, 269, 504
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 3 cut(s) 79, 269, 504
MwoI GCNNNNNNNGC 3 cut(s) 381, 390, 916
NdeII GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
NlaIII CATG 2 cut(s) 176, 1060
NmuCI GTSAC 2 cut(s) 68, 134
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 1 cut(s) 176
PaqCI CACCTGC 2 cut(s) 439, 463
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 5 cut(s) 218, 419, 446, 470, 494
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 4 cut(s) 353, 620, 996, 1099
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 2 cut(s) 518, 533
Psp124BI GAGCTC 1 cut(s) 1068
Psp6I CCWGG 3 cut(s) 77, 267, 502
PspGI CCWGG 3 cut(s) 77, 267, 502
PspPI GGNCC 2 cut(s) 670, 806
PstNI CAGNNNCTG 2 cut(s) 994, 1097
PsuI RGATCY 2 cut(s) 710, 1047
RsaI GTAC 2 cut(s) 341, 1054
RsaNI GTAC 2 cut(s) 340, 1053
RseI CAYNNNNRTG 1 cut(s) 796
SacI GAGCTC 1 cut(s) 1068
SaqAI TTAA 2 cut(s) 366, 675
SatI GCNGC 4 cut(s) 352, 619, 995, 1098
Sau3AI GATC 7 cut(s) 14, 107, 127, 252, 540, 710, 1047
Sau96I GGNCC 2 cut(s) 670, 806
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 79, 269, 504
SduI GDGCHC 3 cut(s) 777, 828, 1068
SfaNI GCATC 4 cut(s) 413, 443, 467, 928
SinI GGWCC 1 cut(s) 670
SmiMI CAYNNNNRTG 1 cut(s) 796
SmlI CTYRAG 2 cut(s) 84, 103
SmoI CTYRAG 2 cut(s) 84, 103
SnaBI TACGTA 2 cut(s) 518, 533
Sse9I AATT 5 cut(s) 677, 857, 864, 1019, 1071
SsiI CCGC 3 cut(s) 352, 382, 611
SspMI CTAG 2 cut(s) 714, 834
SstI GAGCTC 1 cut(s) 1068
StyD4I CCNGG 3 cut(s) 77, 267, 502
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 332, 339
TaiI ACGT 3 cut(s) 520, 535, 630
TaqI TCGA 1 cut(s) 17
TasI AATT 5 cut(s) 677, 857, 864, 1019, 1071
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 5 cut(s) 218, 419, 446, 470, 494
Tru1I TTAA 2 cut(s) 366, 675
Tru9I TTAA 2 cut(s) 366, 675
TscAI CASTG 3 cut(s) 210, 725, 746
TseFI GTSAC 2 cut(s) 68, 134
TseI GCWGC 3 cut(s) 618, 994, 1097
Tsp45I GTSAC 2 cut(s) 68, 134
TspDTI ATGAA 3 cut(s) 17, 184, 250
TspRI CASTG 3 cut(s) 210, 725, 746
VpaK11BI GGWCC 1 cut(s) 670
XceI RCATGY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XspI CTAG 2 cut(s) 714, 834
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.