Rw3G001830

Ubiquitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0019909
Physical Location & Seq
Forward (+)
1578885 .. 1580743
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001830.1

Sequence Viewer

Length: 1359 bp
ATGACGTTACACAGAGATGGTGCCATCGACATCACTCTTGACTTTGGAGATATGTGTCCATTTACGAGCACTGGAGCTGAATTTAAGGGCACTGGAACTAATTTTAAGTTCTATGGAAATCATGAGGCTACCATTAAGATGTACACAAGTGACACCCTCAGGAAGTTGAGAGAAGAGATACTAAGGCACTTTGATGAACATTTGGGGATCGAATTACGTTTTGGTGAAGAAGTGATATTGAAGTTAGACGGCGATACTGTGTCCTTTCGGGATTGTGACATTAGCAGGACTCAAGATATCAATGTTATCTTGAGATCACCACCACCAATCTATGATCTCGGTGACTTGAGTTCTGTTGGGGAGATAGATGTGATAGTACATGCAAAAGGATTTCCAAGGTTCATTGTTCCAGTGTTTTTGCTGGATTCTGTGCAAGACATCAAGGAAAAGATTATGATGATCACATTCATTGACCAGGAGAACCAAATGCTGTATTACAGAAAGAAGGTGCTTGAAGAGGATAGCATACTTGCCTACTGTGGACTGTACAACAACTGCCGCATTCAGTTAGTTAATACTGGCAAACCCCGCGAACCCTGCAAACCCTCTGCATCTGAGCCTGAATCATCAACACCTGCATCTGAGCCTGAATCAACACCTGCATCTGAGCCTGAATCAACACCTGCATCTGTGCCTGAATCAACACCTGTATCTGTGCCTGAATCAACACCTGGAGCTACTATACGTAATGAGATCATTCCTGGAGCTACTATACGTAATGAGATCATTCGTGTCTGCCTCTATTACGGGGACTTCAACCTGCCTCTTCCCTCTGCTACCTTTGAAGTGAAGGCGGATAGCTGCCTCAACGTATTGAAAGCAGAGATATTGAAGCAGGTTGAGAGCTACTTGGTCCTTAAATTGCCCTCTGCTGAAGAAGTAAGAGTAGGTGGATCTAGTCAGTGTGATATAACTCTTGCCCAGTGTCAGCTTTTTGGAATGGCAAATATCAAGGTGCTCTTGAAACAACCACCACCAGAATGGCTTGGCCCTTATTGTGGAGGAGTGCTCTCTACTAGGTCTATGATGACTTTGAAAGAATTGGTAATTTTACGAAATGGAACAGATAGATGGCCAGCAGTAAGGTTCAAGCTTCGTATGCATCCTCAGAGAATGGTTCTACATATAAAGGAAGTGATTGAGAAGATTTCCAATGCTAAAAAGCGCTATCAGAGGCTGCTGAACTATGCAACTGAAGAAGAATTGTTTGATTATTCCCAGATTTCCCAGATCTGTACCATGATAGAGCTCAGAATTAGTGTTCAAGGTTTATCAGAGGCTGCTAAACTATGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

452

Amino Acids

50.82

Weight (kDa)

5.41

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 134 - 191 3.5e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019909)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0449871
rosa_laevigata RLG00000025777
rosa_samantha Rh3AG022700 Rh3BG023400 Rh3CG022400 Rh3DG023300
rosa_wichuraiana Rw3G001830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 643, 667, 691
Acc36I ACCTGC 5 cut(s) 643, 667, 691, 828, 886
AccB1I GGYRCC 1 cut(s) 20
AccII CGCG 1 cut(s) 591
AciI CCGC 3 cut(s) 559, 589, 854
AclWI GGATC 2 cut(s) 215, 961
AcoI YGGCCR 1 cut(s) 1133
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 2 cut(s) 954, 1275
AfaI GTAC 4 cut(s) 143, 378, 548, 1297
AfeI AGCGCT 1 cut(s) 1226
AfiI CCNNNNNNNGG 1 cut(s) 1058
AjnI CCWGG 3 cut(s) 474, 730, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AluBI AGCT 8 cut(s) 77, 737, 767, 861, 906, 991, 1153, 1309
AluI AGCT 8 cut(s) 77, 737, 767, 861, 906, 991, 1153, 1309
Alw21I GWGCWC 4 cut(s) 71, 1020, 1071, 1311
AlwI GGATC 2 cut(s) 215, 961
AlwNI CAGNNNCTG 2 cut(s) 1237, 1340
Aor51HI AGCGCT 1 cut(s) 1226
AoxI GGCC 2 cut(s) 1048, 1133
ApeKI GCWGC 3 cut(s) 861, 1237, 1340
ApoI RAATTY 1 cut(s) 80
AspLEI GCGC 1 cut(s) 1227
AspS9I GGNCC 2 cut(s) 913, 1049
AsuHPI GGTGA 3 cut(s) 236, 309, 353
AvaII GGWCC 1 cut(s) 913
AxyI CCTNAGG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BalI TGGCCA 1 cut(s) 1135
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 1311
Bbv12I GWGCWC 4 cut(s) 71, 1020, 1071, 1311
BbvI GCAGC 3 cut(s) 848, 1224, 1327
BccI CCATC 3 cut(s) 11, 32, 1125
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 3 cut(s) 476, 732, 762
BclI TGATCA 1 cut(s) 459
BfaI CTAG 2 cut(s) 957, 1077
BfoI RGCGCY 1 cut(s) 1228
BfuAI ACCTGC 5 cut(s) 643, 667, 691, 828, 886
BglII AGATCT 1 cut(s) 1290
BisI GCNGC 4 cut(s) 559, 862, 1238, 1341
BlsI GCNGC 4 cut(s) 560, 863, 1239, 1342
Bme1390I CCNGG 3 cut(s) 476, 732, 762
Bme18I GGWCC 1 cut(s) 913
BmgT120I GGNCC 2 cut(s) 913, 1049
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 3 cut(s) 476, 732, 762
BmrI ACTGGG 1 cut(s) 976
BmsI GCATC 5 cut(s) 620, 647, 671, 695, 1171
BmuI ACTGGG 1 cut(s) 976
BplI GAGNNNNNCTC 2 cut(s) 1053, 1085
BpmI CTGGAG 3 cut(s) 93, 753, 783
BpuEI CTTGAG 3 cut(s) 276, 331, 367
BsaAI YACGTR 2 cut(s) 746, 776
BsaJI CCNNGG 1 cut(s) 395
Bsc4I CCNNNNNNNGG 1 cut(s) 1058
Bse1I ACTGG 5 cut(s) 76, 97, 410, 583, 982
Bse21I CCTNAGG 1 cut(s) 158
BseBI CCWGG 3 cut(s) 476, 732, 762
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 1 cut(s) 1162
BseLI CCNNNNNNNGG 1 cut(s) 1058
BseMII CTCAG 6 cut(s) 172, 606, 633, 657, 1181, 1324
BseNI ACTGG 5 cut(s) 76, 97, 410, 583, 982
BseRI GAGGAG 1 cut(s) 1077
BseSI GKGCMC 1 cut(s) 92
BseXI GCAGC 3 cut(s) 848, 1224, 1327
Bsh1236I CGCG 1 cut(s) 591
BshFI GGCC 2 cut(s) 1050, 1135
BshNI GGYRCC 1 cut(s) 20
BsiHKAI GWGCWC 4 cut(s) 71, 1020, 1071, 1311
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 1 cut(s) 1058
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 1 cut(s) 561
BsnI GGCC 2 cut(s) 1050, 1135
Bsp1286I GDGCHC 5 cut(s) 71, 92, 1020, 1071, 1311
Bsp1407I TGTACA 2 cut(s) 141, 546
Bsp143I GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
BspACI CCGC 3 cut(s) 559, 589, 854
BspANI GGCC 2 cut(s) 1050, 1135
BspCNI CTCAG 6 cut(s) 171, 607, 634, 658, 1180, 1323
BspFNI CGCG 1 cut(s) 591
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 5 cut(s) 643, 667, 691, 828, 886
BspPI GGATC 2 cut(s) 215, 961
BspT107I GGYRCC 1 cut(s) 20
BsrGI TGTACA 2 cut(s) 141, 546
BsrI ACTGG 5 cut(s) 76, 97, 410, 583, 982
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
BssT1I CCWWGG 1 cut(s) 395
Bst2UI CCWGG 3 cut(s) 476, 732, 762
Bst4CI ACNGT 3 cut(s) 259, 539, 546
Bst6I CTCTTC 3 cut(s) 168, 510, 831
BstAUI TGTACA 2 cut(s) 141, 546
BstBAI YACGTR 2 cut(s) 746, 776
BstC8I GCNNGC 1 cut(s) 1137
BstDEI CTNAG 7 cut(s) 158, 182, 615, 642, 666, 1167, 1310
BstF5I GGATG 1 cut(s) 1162
BstFNI CGCG 1 cut(s) 591
BstH2I RGCGCY 1 cut(s) 1228
BstHHI GCGC 1 cut(s) 1227
BstKTI GATC 8 cut(s) 210, 317, 337, 462, 756, 786, 956, 1293
BstMBI GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
BstMWI GCNNNNNNNGC 3 cut(s) 588, 597, 1159
BstNI CCWGG 3 cut(s) 476, 732, 762
BstNSI RCATGY 1 cut(s) 383
BstSCI CCNGG 3 cut(s) 474, 730, 760
BstSLI GKGCMC 1 cut(s) 92
BstSNI TACGTA 2 cut(s) 746, 776
BstUI CGCG 1 cut(s) 591
BstV1I GCAGC 3 cut(s) 848, 1224, 1327
BstX2I RGATCY 2 cut(s) 953, 1290
BstXI CCANNNNNNTGG 1 cut(s) 1041
BstYI RGATCY 2 cut(s) 953, 1290
Bsu36I CCTNAGG 1 cut(s) 158
BsuRI GGCC 2 cut(s) 1050, 1135
BtsCI GGATG 1 cut(s) 1162
BtsIMutI CAGTG 6 cut(s) 69, 90, 417, 968, 989, 1354
BveI ACCTGC 5 cut(s) 643, 667, 691, 828, 886
Cac8I GCNNGC 1 cut(s) 1137
CaiI CAGNNNCTG 2 cut(s) 1237, 1340
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 1227
Cfr13I GGNCC 2 cut(s) 913, 1049
Csp6I GTAC 4 cut(s) 142, 377, 547, 1296
CviAII CATG 3 cut(s) 122, 380, 1300
CviQI GTAC 4 cut(s) 142, 377, 547, 1296
DdeI CTNAG 7 cut(s) 158, 182, 615, 642, 666, 1167, 1310
DpnI GATC 8 cut(s) 209, 316, 336, 461, 755, 785, 955, 1292
DpnII GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
EaeI YGGCCR 1 cut(s) 1133
Eam1104I CTCTTC 3 cut(s) 168, 510, 831
EarI CTCTTC 3 cut(s) 168, 510, 831
EciI GGCGGA 1 cut(s) 869
Ecl136II GAGCTC 1 cut(s) 1309
Eco105I TACGTA 2 cut(s) 746, 776
Eco130I CCWWGG 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 1311
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 913
Eco47III AGCGCT 1 cut(s) 1226
Eco53kI GAGCTC 1 cut(s) 1309
Eco57I CTGAAG 2 cut(s) 954, 1275
Eco81I CCTNAGG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 1309
EcoRII CCWGG 3 cut(s) 474, 730, 760
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 395
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 1 cut(s) 1311
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 3 cut(s) 125, 383, 1303
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FaqI GGGAC 1 cut(s) 824
FatI CATG 3 cut(s) 121, 379, 1299
FauI CCCGC 1 cut(s) 596
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 4 cut(s) 559, 862, 1238, 1341
FokI GGATG 1 cut(s) 1149
FriOI GRGCYC 1 cut(s) 1311
Fsp4HI GCNGC 4 cut(s) 559, 862, 1238, 1341
FspBI CTAG 2 cut(s) 957, 1077
GlaI GCGC 1 cut(s) 1226
GluI GCNGC 4 cut(s) 559, 862, 1238, 1341
GsuI CTGGAG 3 cut(s) 93, 753, 783
HaeII RGCGCY 1 cut(s) 1228
HaeIII GGCC 2 cut(s) 1050, 1135
HhaI GCGC 1 cut(s) 1227
Hin1II CATG 3 cut(s) 125, 383, 1303
Hin6I GCGC 1 cut(s) 1225
HinP1I GCGC 1 cut(s) 1225
HindIII AAGCTT 1 cut(s) 1151
HinfI GANTC 7 cut(s) 289, 425, 623, 650, 674, 698, 722
HphI GGTGA 3 cut(s) 236, 309, 353
Hpy166II GTNNAC 2 cut(s) 144, 542
Hpy188I TCNGA 7 cut(s) 616, 643, 667, 1170, 1233, 1313, 1336
Hpy188III TCNNGA 7 cut(s) 38, 122, 160, 269, 293, 310, 1021
Hpy8I GTNNAC 2 cut(s) 144, 542
HpyAV CCTTC 2 cut(s) 499, 844
HpyCH4III ACNGT 3 cut(s) 259, 539, 546
HpyCH4IV ACGT 5 cut(s) 5, 217, 745, 775, 870
HpyCH4V TGCA 9 cut(s) 383, 433, 600, 611, 638, 662, 686, 1162, 1250
HpyF10VI GCNNNNNNNGC 3 cut(s) 588, 597, 1159
HpyF3I CTNAG 7 cut(s) 158, 182, 615, 642, 666, 1167, 1310
HpySE526I ACGT 5 cut(s) 5, 217, 745, 775, 870
Hsp92II CATG 3 cut(s) 125, 383, 1303
HspAI GCGC 1 cut(s) 1225
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
LmnI GCTCC 3 cut(s) 74, 734, 764
Lsp1109I GCAGC 3 cut(s) 848, 1224, 1327
LweI GCATC 5 cut(s) 620, 647, 671, 695, 1171
MaeI CTAG 2 cut(s) 957, 1077
MaeII ACGT 5 cut(s) 5, 217, 745, 775, 870
MaeIII GTNAC 4 cut(s) 6, 149, 275, 341
MalI GATC 8 cut(s) 209, 316, 336, 461, 755, 785, 955, 1292
MboI GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
MboII GAAGA 8 cut(s) 185, 239, 527, 818, 947, 1216, 1268, 1271
MflI RGATCY 2 cut(s) 953, 1290
MhlI GDGCHC 5 cut(s) 71, 92, 1020, 1071, 1311
MlsI TGGCCA 1 cut(s) 1135
MluCI AATT 8 cut(s) 80, 100, 212, 920, 1100, 1107, 1262, 1314
MluNI TGGCCA 1 cut(s) 1135
MlyI GAGTC 1 cut(s) 283
Mox20I TGGCCA 1 cut(s) 1135
Mph1103I ATGCAT 1 cut(s) 1164
MscI TGGCCA 1 cut(s) 1135
MseI TTAA 5 cut(s) 84, 105, 135, 573, 918
MslI CAYNNNNRTG 4 cut(s) 15, 137, 192, 1039
Msp20I TGGCCA 1 cut(s) 1135
MspR9I CCNGG 3 cut(s) 476, 732, 762
Mva1269I GAATGC 1 cut(s) 561
MvaI CCWGG 3 cut(s) 476, 732, 762
MvnI CGCG 1 cut(s) 591
MwoI GCNNNNNNNGC 3 cut(s) 588, 597, 1159
NdeII GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
NlaIII CATG 3 cut(s) 125, 383, 1303
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 3 cut(s) 149, 275, 341
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 1 cut(s) 383
PagI TCATGA 1 cut(s) 121
PaqCI CACCTGC 3 cut(s) 643, 667, 691
PctI GAATGC 1 cut(s) 561
PfeI GAWTC 6 cut(s) 425, 623, 650, 674, 698, 722
PfoI TCCNGGA 1 cut(s) 760
PkrI GCNGC 4 cut(s) 560, 863, 1239, 1342
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Ppu21I YACGTR 2 cut(s) 746, 776
Psp124BI GAGCTC 1 cut(s) 1311
Psp6I CCWGG 3 cut(s) 474, 730, 760
PspGI CCWGG 3 cut(s) 474, 730, 760
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 913, 1049
PstNI CAGNNNCTG 2 cut(s) 1237, 1340
PsuI RGATCY 2 cut(s) 953, 1290
RsaI GTAC 4 cut(s) 143, 378, 548, 1297
RsaNI GTAC 4 cut(s) 142, 377, 547, 1296
RseI CAYNNNNRTG 4 cut(s) 15, 137, 192, 1039
SacI GAGCTC 1 cut(s) 1311
SaqAI TTAA 5 cut(s) 84, 105, 135, 573, 918
SatI GCNGC 4 cut(s) 559, 862, 1238, 1341
Sau3AI GATC 8 cut(s) 207, 314, 334, 459, 753, 783, 953, 1290
Sau96I GGNCC 2 cut(s) 913, 1049
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 3 cut(s) 476, 732, 762
SduI GDGCHC 5 cut(s) 71, 92, 1020, 1071, 1311
SfaNI GCATC 5 cut(s) 620, 647, 671, 695, 1171
SinI GGWCC 1 cut(s) 913
SmiMI CAYNNNNRTG 4 cut(s) 15, 137, 192, 1039
SmlI CTYRAG 3 cut(s) 291, 310, 346
SmoI CTYRAG 3 cut(s) 291, 310, 346
SnaBI TACGTA 2 cut(s) 746, 776
Sse9I AATT 8 cut(s) 80, 100, 212, 920, 1100, 1107, 1262, 1314
SsiI CCGC 3 cut(s) 559, 589, 854
SspMI CTAG 2 cut(s) 957, 1077
SstI GAGCTC 1 cut(s) 1311
StyD4I CCNGG 3 cut(s) 474, 730, 760
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 3 cut(s) 259, 539, 546
TaiI ACGT 5 cut(s) 8, 220, 748, 778, 873
TaqI TCGA 2 cut(s) 27, 210
TasI AATT 8 cut(s) 80, 100, 212, 920, 1100, 1107, 1262, 1314
TatI WGTACW 3 cut(s) 141, 376, 546
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 6 cut(s) 425, 623, 650, 674, 698, 722
Tru1I TTAA 5 cut(s) 84, 105, 135, 573, 918
Tru9I TTAA 5 cut(s) 84, 105, 135, 573, 918
TscAI CASTG 5 cut(s) 76, 97, 417, 968, 989
TseFI GTSAC 3 cut(s) 149, 275, 341
TseI GCWGC 3 cut(s) 861, 1237, 1340
Tsp45I GTSAC 3 cut(s) 149, 275, 341
TspDTI ATGAA 3 cut(s) 210, 391, 457
TspRI CASTG 5 cut(s) 76, 97, 417, 968, 989
VpaK11BI GGWCC 1 cut(s) 913
XapI RAATTY 1 cut(s) 80
XceI RCATGY 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 1038
XspI CTAG 2 cut(s) 957, 1077
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.