Rh3AG022700

Ubiquitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0019909
Physical Location & Seq
Forward (+)
1526650 .. 1529052
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG022700.1

Sequence Viewer

Length: 906 bp
ATGAACATTTGGGGATCGAAAGAAGTGATATTGAAGTTAGACGGCGATACTCTGTCCTTTCGGGATTGTGACATTTCCAGGACTCAAGATATCAATGTTATCTTGAGATCACCACCACCAATCTATGATCTCGGTGACTTGACTTCTGTTGGGGAGATAGATGTGATAATACATGCAAAAGGATTTCCAAGGTTCATTGTTCCAGTGTTTTTGCTGGATTCTGTGCAAGACATCAAGGAAAAGATTATGATGATCACATTCATTGACCAGGAGAACCAAATGCTGTATTACAGAAAGAAGGTGCTTGAAGAGGATAGCATACTTGCCTACTGTGGACTGTACAACAACTGCCGCATTCAGTTAGTTAATACTGGCAAACCCCGCAAACCCTGCAAACCCTCTGCATCATCTGAGCCTGAATCATCAACACCTGCATCTGAGCCTGAATCAACACCTGCATCTGAGCCTGAATCAACACCTGTATCTGTGCCTGAATCAACACCTGGAGCTACTATACGTAATGATACTATACGTAATGAGATCATTCGTGTCTGCCTCTATTACGGGGACTTCAACCTGCCTCTTCCCTCTGCTACCTTTGAAGTGAAGGCGGATAGCTGCCTCAACGTATTGAAAGCAGAGATATTGAAGCAGGTTGAGAGCTACTTGGTCCTTAAATTGCCCTCTGCTGAAGAAGTAAGAGTAGGTGGATCTAGTCAGTGTGATATATCTCTTGCCCAGTGTCAGCTTTTTGGAATGGCAAATATCAAGGTGCTCTTGAAACAACCACCACCAGAATGGCTTGGCCCTTATTGTGGAGGAGTGCTCTCTACTAGGTACATTTATATATACACGTCTTTTGAATTCTCAATTGCATACATAAATATGTCAAAATGTTCCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

301

Amino Acids

33.55

Weight (kDa)

4.93

Isoelectric Point (pI)

52.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 65 - 122 2e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019909)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0449871
rosa_laevigata RLG00000025777
rosa_samantha Rh3AG022700 Rh3BG023400 Rh3CG022400 Rh3DG023300
rosa_wichuraiana Rw3G001830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 439, 463
Acc36I ACCTGC 4 cut(s) 439, 463, 585, 643
AciI CCGC 3 cut(s) 352, 382, 611
AclWI GGATC 2 cut(s) 22, 718
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 1 cut(s) 711
AfaI GTAC 2 cut(s) 341, 839
AfiI CCNNNNNNNGG 1 cut(s) 815
AflIII ACRYGT 1 cut(s) 852
AgsI TTSAA 8 cut(s) 34, 308, 574, 602, 634, 649, 781, 863
AjiI CACGTC 1 cut(s) 855
AjnI CCWGG 3 cut(s) 77, 267, 502
AluBI AGCT 4 cut(s) 509, 618, 663, 748
AluI AGCT 4 cut(s) 509, 618, 663, 748
Alw21I GWGCWC 2 cut(s) 777, 828
AlwI GGATC 2 cut(s) 22, 718
AoxI GGCC 1 cut(s) 805
ApeKI GCWGC 1 cut(s) 618
ApoI RAATTY 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 670, 806
AsuHPI GGTGA 2 cut(s) 102, 146
AvaII GGWCC 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
Bbv12I GWGCWC 2 cut(s) 777, 828
BbvI GCAGC 1 cut(s) 605
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 3 cut(s) 79, 269, 504
BclI TGATCA 1 cut(s) 252
BfaI CTAG 2 cut(s) 714, 834
BfuAI ACCTGC 4 cut(s) 439, 463, 585, 643
BisI GCNGC 2 cut(s) 352, 619
BlsI GCNGC 2 cut(s) 353, 620
Bme1390I CCNGG 3 cut(s) 79, 269, 504
Bme18I GGWCC 1 cut(s) 670
BmgBI CACGTC 1 cut(s) 855
BmgT120I GGNCC 2 cut(s) 670, 806
BmrFI CCNGG 3 cut(s) 79, 269, 504
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 3 cut(s) 413, 443, 467
BmuI ACTGGG 1 cut(s) 733
BplI GAGNNNNNCTC 2 cut(s) 810, 842
BpmI CTGGAG 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 69, 124
BsaAI YACGTR 2 cut(s) 518, 533
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 815
Bse1I ACTGG 3 cut(s) 203, 376, 739
BseBI CCWGG 3 cut(s) 79, 269, 504
BseDI CCNNGG 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 815
BseMII CTCAG 3 cut(s) 402, 429, 453
BseNI ACTGG 3 cut(s) 203, 376, 739
BseRI GAGGAG 1 cut(s) 834
BseXI GCAGC 1 cut(s) 605
BshFI GGCC 1 cut(s) 807
BsiHKAI GWGCWC 2 cut(s) 777, 828
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 1 cut(s) 815
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 1 cut(s) 807
Bsp1286I GDGCHC 2 cut(s) 777, 828
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 6 cut(s) 14, 107, 127, 252, 540, 710
BspACI CCGC 3 cut(s) 352, 382, 611
BspANI GGCC 1 cut(s) 807
BspCNI CTCAG 3 cut(s) 403, 430, 454
BspMI ACCTGC 4 cut(s) 439, 463, 585, 643
BspPI GGATC 2 cut(s) 22, 718
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 3 cut(s) 203, 376, 739
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 6 cut(s) 14, 107, 127, 252, 540, 710
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 79, 269, 504
Bst4CI ACNGT 2 cut(s) 332, 339
Bst6I CTCTTC 2 cut(s) 303, 588
BstAPI GCANNNNNTGC 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 2 cut(s) 518, 533
BstDEI CTNAG 3 cut(s) 411, 438, 462
BstKTI GATC 6 cut(s) 17, 110, 130, 255, 543, 713
BstMBI GATC 6 cut(s) 14, 107, 127, 252, 540, 710
BstMWI GCNNNNNNNGC 2 cut(s) 381, 390
BstNI CCWGG 3 cut(s) 79, 269, 504
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 3 cut(s) 77, 267, 502
BstSNI TACGTA 2 cut(s) 518, 533
BstV1I GCAGC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 710
BstXI CCANNNNNNTGG 1 cut(s) 798
BstYI RGATCY 1 cut(s) 710
BsuRI GGCC 1 cut(s) 807
BtrI CACGTC 1 cut(s) 855
BtsIMutI CAGTG 3 cut(s) 210, 725, 746
BveI ACCTGC 4 cut(s) 439, 463, 585, 643
Cfr13I GGNCC 2 cut(s) 670, 806
Csp6I GTAC 2 cut(s) 340, 838
CviAII CATG 1 cut(s) 173
CviJI RGCY 9 cut(s) 415, 442, 466, 509, 618, 663, 748, 802, 807
CviKI_1 RGCY 9 cut(s) 415, 442, 466, 509, 618, 663, 748, 802, 807
CviQI GTAC 2 cut(s) 340, 838
DdeI CTNAG 3 cut(s) 411, 438, 462
DpnI GATC 6 cut(s) 16, 109, 129, 254, 542, 712
DpnII GATC 6 cut(s) 14, 107, 127, 252, 540, 710
Eam1104I CTCTTC 2 cut(s) 303, 588
EarI CTCTTC 2 cut(s) 303, 588
EciI GGCGGA 1 cut(s) 626
Eco105I TACGTA 2 cut(s) 518, 533
Eco130I CCWWGG 1 cut(s) 188
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 711
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 3 cut(s) 77, 267, 502
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 1 cut(s) 176
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 581
FatI CATG 1 cut(s) 172
FauI CCCGC 1 cut(s) 389
FbaI TGATCA 1 cut(s) 252
Fnu4HI GCNGC 2 cut(s) 352, 619
Fsp4HI GCNGC 2 cut(s) 352, 619
FspBI CTAG 2 cut(s) 714, 834
GluI GCNGC 2 cut(s) 352, 619
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 1 cut(s) 807
Hin1II CATG 1 cut(s) 176
HinfI GANTC 6 cut(s) 82, 218, 419, 446, 470, 494
HphI GGTGA 2 cut(s) 102, 146
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 3 cut(s) 412, 439, 463
Hpy188III TCNNGA 4 cut(s) 62, 86, 103, 778
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 2 cut(s) 292, 601
HpyCH4III ACNGT 2 cut(s) 332, 339
HpyCH4IV ACGT 4 cut(s) 517, 532, 627, 854
HpyCH4V TGCA 7 cut(s) 176, 226, 393, 404, 434, 458, 875
HpyF10VI GCNNNNNNNGC 2 cut(s) 381, 390
HpyF3I CTNAG 3 cut(s) 411, 438, 462
HpySE526I ACGT 4 cut(s) 517, 532, 627, 854
Hsp92II CATG 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 6 cut(s) 14, 107, 127, 252, 540, 710
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 3 cut(s) 413, 443, 467
MaeI CTAG 2 cut(s) 714, 834
MaeII ACGT 4 cut(s) 517, 532, 627, 854
MaeIII GTNAC 2 cut(s) 68, 134
MalI GATC 6 cut(s) 16, 109, 129, 254, 542, 712
MboI GATC 6 cut(s) 14, 107, 127, 252, 540, 710
MboII GAAGA 3 cut(s) 320, 575, 704
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 2 cut(s) 777, 828
MluCI AATT 3 cut(s) 677, 863, 870
MlyI GAGTC 1 cut(s) 76
MnlI CCTC 8 cut(s) 304, 409, 566, 591, 598, 632, 694, 812
MseI TTAA 3 cut(s) 366, 675, 904
MslI CAYNNNNRTG 2 cut(s) 796, 884
MspR9I CCNGG 3 cut(s) 79, 269, 504
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 3 cut(s) 79, 269, 504
MwoI GCNNNNNNNGC 2 cut(s) 381, 390
NdeII GATC 6 cut(s) 14, 107, 127, 252, 540, 710
NlaIII CATG 1 cut(s) 176
NmuCI GTSAC 2 cut(s) 68, 134
NspI RCATGY 1 cut(s) 176
PaqCI CACCTGC 2 cut(s) 439, 463
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 5 cut(s) 218, 419, 446, 470, 494
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 2 cut(s) 353, 620
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 2 cut(s) 518, 533
Psp6I CCWGG 3 cut(s) 77, 267, 502
PspGI CCWGG 3 cut(s) 77, 267, 502
PspPI GGNCC 2 cut(s) 670, 806
PsuI RGATCY 1 cut(s) 710
RsaI GTAC 2 cut(s) 341, 839
RsaNI GTAC 2 cut(s) 340, 838
RseI CAYNNNNRTG 2 cut(s) 796, 884
SaqAI TTAA 3 cut(s) 366, 675, 904
SatI GCNGC 2 cut(s) 352, 619
Sau3AI GATC 6 cut(s) 14, 107, 127, 252, 540, 710
Sau96I GGNCC 2 cut(s) 670, 806
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 79, 269, 504
SduI GDGCHC 2 cut(s) 777, 828
SfaNI GCATC 3 cut(s) 413, 443, 467
SinI GGWCC 1 cut(s) 670
SmiMI CAYNNNNRTG 2 cut(s) 796, 884
SmlI CTYRAG 2 cut(s) 84, 103
SmoI CTYRAG 2 cut(s) 84, 103
SnaBI TACGTA 2 cut(s) 518, 533
Sse9I AATT 3 cut(s) 677, 863, 870
SsiI CCGC 3 cut(s) 352, 382, 611
SspMI CTAG 2 cut(s) 714, 834
StyD4I CCNGG 3 cut(s) 77, 267, 502
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 332, 339
TaiI ACGT 4 cut(s) 520, 535, 630, 857
TaqI TCGA 1 cut(s) 17
TasI AATT 3 cut(s) 677, 863, 870
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 5 cut(s) 218, 419, 446, 470, 494
Tru1I TTAA 3 cut(s) 366, 675, 904
Tru9I TTAA 3 cut(s) 366, 675, 904
TscAI CASTG 3 cut(s) 210, 725, 746
TseFI GTSAC 2 cut(s) 68, 134
TseI GCWGC 1 cut(s) 618
Tsp45I GTSAC 2 cut(s) 68, 134
TspDTI ATGAA 3 cut(s) 17, 184, 250
TspRI CASTG 3 cut(s) 210, 725, 746
VpaK11BI GGWCC 1 cut(s) 670
XapI RAATTY 1 cut(s) 863
XceI RCATGY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XspI CTAG 2 cut(s) 714, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.