RLG00000027334

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018077
Physical Location & Seq
Reverse (-)
10710477 .. 10711643
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027334

Sequence Viewer

Length: 525 bp
ATGCTTTCCTTCAAAATCACTCGTATTATTTCTCTGATATTGAGGATCCTGAGCGCCATTGCGCTCTTTACGTCCATCGTCATCCTTGGCATCAATAATATCGATCTGATTCCTTACAAGAACGGAAAGCCTGCAGGAAAACCATACACCGCCCACTTTTATGACATAGTAGGGTTCCGATACATTTTTGCTACAGCTTTTATTGGAACTGCATATTCAATCGGCCAGGGTGTATGTACAATTATCCATTTGGCGAAAGGAAAAGAAGTAGGGCACGTCACCTTTGATTTCTATGCTGAGGAGGTTATGTCAAATTTTTTAGCTTCAGGAGCTGTCGCAGGGTTTATCACGGCTGCGCAGTACTTGAAAATGTGGGATGAAATTAATTCGCTAATAGACCACCGCTTTATCAATATGACATATATAGCATCTGGACTTGTCATCTTTGCCTTCCTCAGCAGTTTCGTAATATCAATATTCTCTTCATATGCTCTCGGTAGAAATGATGATGATGAGCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

175

Amino Acids

19.26

Weight (kDa)

6.39

Isoelectric Point (pI)

26.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 152 2.1e-13 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018077)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g22220
prunus_persica Prupe.2G234100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366601
rosa_laevigata RLG00000027334
rosa_roxburghii Rroxscaffold_4G00289820
rosa_rugosa Rorug01G0330800
rosa_samantha Rh1AG339200 Rh1BG300800 Rh1CG315700 Rh1DG332500
rosa_wichuraiana Rw1G030040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 357
AciI CCGC 2 cut(s) 150, 403
AclWI GGATC 2 cut(s) 40, 53
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 238, 362
AgsI TTSAA 3 cut(s) 13, 219, 367
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 4 cut(s) 197, 323, 332, 517
AluI AGCT 4 cut(s) 197, 323, 332, 517
AlwI GGATC 2 cut(s) 40, 53
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 313
AseI ATTAAT 1 cut(s) 384
AspLEI GCGC 3 cut(s) 56, 64, 358
AsuHPI GGTGA 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 45
BbvCI CCTCAGC 2 cut(s) 297, 455
BbvI GCAGC 1 cut(s) 340
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 366
BciT130I CCWGG 1 cut(s) 227
BfmI CTRYAG 2 cut(s) 132, 192
BfoI RGCGCY 1 cut(s) 57
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 47, 176
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 99, 437
Bpu10I CCTNAGC 3 cut(s) 50, 297, 455
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 85, 226
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse3DI GCAATG 1 cut(s) 57
BseBI CCWGG 1 cut(s) 227
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 85, 226
BseGI GGATG 2 cut(s) 81, 382
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 3 cut(s) 41, 288, 469
BseRI GAGGAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 1 cut(s) 340
BshFI GGCC 1 cut(s) 225
BshVI ATCGAT 1 cut(s) 102
BsnI GGCC 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 276
Bsp1407I TGTACA 1 cut(s) 236
Bsp143I GATC 2 cut(s) 45, 103
BspACI CCGC 2 cut(s) 150, 403
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 3 cut(s) 42, 289, 468
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 47, 176
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 2 cut(s) 40, 53
BsrDI GCAATG 1 cut(s) 57
BsrGI TGTACA 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 85, 226
BssMI GATC 2 cut(s) 45, 103
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 487
BstAUI TGTACA 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 3 cut(s) 50, 297, 455
BstF5I GGATG 2 cut(s) 81, 382
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 3 cut(s) 56, 64, 358
BstKTI GATC 2 cut(s) 48, 106
BstMBI GATC 2 cut(s) 45, 103
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 2 cut(s) 132, 192
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 102
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 2 cut(s) 81, 382
Cac8I GCNNGC 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 3 cut(s) 56, 64, 358
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 2 cut(s) 237, 361
CviJI RGCY 7 cut(s) 130, 197, 225, 323, 332, 353, 517
CviKI_1 RGCY 7 cut(s) 130, 197, 225, 323, 332, 353, 517
CviQI GTAC 2 cut(s) 237, 361
DdeI CTNAG 3 cut(s) 50, 297, 455
DpnI GATC 2 cut(s) 47, 105
DpnII GATC 2 cut(s) 45, 103
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 85
FauNDI CATATG 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 2 cut(s) 68, 389
Fsp4HI GCNGC 1 cut(s) 354
FspI TGCGCA 1 cut(s) 357
GlaI GCGC 3 cut(s) 55, 63, 357
GluI GCNGC 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 1 cut(s) 225
HhaI GCGC 3 cut(s) 56, 64, 358
Hin6I GCGC 3 cut(s) 54, 62, 356
HinP1I GCGC 3 cut(s) 54, 62, 356
HinfI GANTC 1 cut(s) 109
HphI GGTGA 1 cut(s) 271
Hpy188I TCNGA 3 cut(s) 36, 108, 179
Hpy188III TCNNGA 3 cut(s) 49, 327, 432
HpyAV CCTTC 2 cut(s) 19, 460
HpyCH4IV ACGT 2 cut(s) 71, 276
HpyCH4V TGCA 2 cut(s) 134, 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 3 cut(s) 50, 297, 455
HpySE526I ACGT 2 cut(s) 71, 276
HspAI GCGC 3 cut(s) 54, 62, 356
Kzo9I GATC 2 cut(s) 45, 103
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 8 cut(s) 62, 120, 144, 212, 239, 312, 324, 417
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 2 cut(s) 99, 437
MaeII ACGT 2 cut(s) 71, 276
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 47, 105
MboI GATC 2 cut(s) 45, 103
MboII GAAGA 1 cut(s) 474
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 4 cut(s) 240, 313, 381, 385
MnlI CCTC 4 cut(s) 36, 292, 295, 464
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeI CATATG 1 cut(s) 487
NdeII GATC 2 cut(s) 45, 103
NlaIV GGNNCC 2 cut(s) 47, 176
NmuCI GTSAC 1 cut(s) 277
NsbI TGCGCA 1 cut(s) 357
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 1 cut(s) 355
PshBI ATTAAT 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 47, 176
PstI CTGCAG 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 2 cut(s) 238, 362
RsaNI GTAC 2 cut(s) 237, 361
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 2 cut(s) 45, 103
SbfI CCTGCAGG 1 cut(s) 136
ScaI AGTACT 1 cut(s) 362
ScrFI CCNGG 1 cut(s) 227
SdaI CCTGCAGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 276
SetI ASST 8 cut(s) 74, 199, 279, 284, 306, 325, 334, 519
SfaNI GCATC 2 cut(s) 99, 437
SfcI CTRYAG 2 cut(s) 132, 192
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse8387I CCTGCAGG 1 cut(s) 136
Sse9I AATT 4 cut(s) 240, 313, 381, 385
SsiI CCGC 2 cut(s) 150, 403
SspI AATATT 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 85
TaiI ACGT 2 cut(s) 74, 279
TaqI TCGA 1 cut(s) 102
TasI AATT 4 cut(s) 240, 313, 381, 385
TatI WGTACW 2 cut(s) 236, 360
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 2 cut(s) 393, 474
TspGWI ACGGA 1 cut(s) 138
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 1 cut(s) 313
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.