Rroxscaffold_4G00289820

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018077
Physical Location & Seq
Reverse (-)
10456854 .. 10458033
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289820.1

Sequence Viewer

Length: 636 bp
ATGCTTTCCTTCAAAATCACTCGTATTATTTCTCTGATATTGAGGATCCTGAGCGCCATTGCGCTCTTTACGTCCATCGTCATCCTTGGCATCAATAATATCGATCTGATTCCTTACAAGAACGGAAAGCCTGCAGGAAAACCCTACACCGCCCACTTTTATGACATAGTAGGGTTCCGATACGTTTTTGCTACAGCTTTTATTGGAACTGCATATTCAATCGGCCAGGGTGTGTGTACAATTATCCATTTGGCGAAAGGAAAAGAAGTAGGGCACGTCACCTTTGATTTCTATGCTGAGGAGGTGAAAACTGATAAACATATTTGCTTCCTTCGACATTGTTCAGTTTTTCTTTCTCGAGTTCGTTTTTCTGAACCCTCCGATTGTTTCCAACACTCGGATTATGTTGCGCAGGTTATGTCAAATTTTTTAGCTTCAGGAGCTGTCGCAGGATTTATCACGGCTGCGCAGTACTTAAAAATGTGGGATGAAATTAATTCGCTAATAGACCACCGCTTTATCAATATGACGTATATAGCATCTGGACTTGTCATCTTTGCCTTCCTCAGCAGTTTCGTAATATCAATATTCTCTTCATATGCACTCGGTAGAAATGATGATGATGAGCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

211

Amino Acids

23.61

Weight (kDa)

7.04

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 93 9.1e-09 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018077)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g22220
prunus_persica Prupe.2G234100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366601
rosa_laevigata RLG00000027334
rosa_roxburghii Rroxscaffold_4G00289820
rosa_rugosa Rorug01G0330800
rosa_samantha Rh1AG339200 Rh1BG300800 Rh1CG315700 Rh1DG332500
rosa_wichuraiana Rw1G030040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 411, 468
Acc36I ACCTGC 1 cut(s) 403
AciI CCGC 2 cut(s) 150, 514
AclWI GGATC 2 cut(s) 40, 53
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 2 cut(s) 238, 473
AfiI CCNNNNNNNGG 1 cut(s) 397
AgsI TTSAA 2 cut(s) 13, 219
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 4 cut(s) 197, 434, 443, 628
AluI AGCT 4 cut(s) 197, 434, 443, 628
AlwI GGATC 2 cut(s) 40, 53
AlwNI CAGNNNCTG 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 357
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 1 cut(s) 424
AseI ATTAAT 1 cut(s) 495
AspLEI GCGC 4 cut(s) 56, 64, 412, 469
AsuHPI GGTGA 2 cut(s) 271, 316
AvaI CYCGRG 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 45
BbvCI CCTCAGC 2 cut(s) 297, 566
BbvI GCAGC 1 cut(s) 451
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 477
BciT130I CCWGG 1 cut(s) 227
BfmI CTRYAG 2 cut(s) 132, 192
BfoI RGCGCY 1 cut(s) 57
BfuAI ACCTGC 1 cut(s) 403
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 357
BmgBI CACGTC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 47, 176
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 99, 548
Bpu10I CCTNAGC 3 cut(s) 50, 297, 566
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 85, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse3DI GCAATG 1 cut(s) 57
BseBI CCWGG 1 cut(s) 227
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 85, 226
BseGI GGATG 2 cut(s) 81, 493
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 3 cut(s) 41, 288, 580
BseRI GAGGAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 1 cut(s) 451
BshFI GGCC 1 cut(s) 225
BshVI ATCGAT 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 397
BsnI GGCC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 276
Bsp1407I TGTACA 1 cut(s) 236
Bsp143I GATC 2 cut(s) 45, 103
BspACI CCGC 2 cut(s) 150, 514
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 3 cut(s) 42, 289, 579
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 47, 176
BspMAI CTGCAG 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 2 cut(s) 40, 53
BsrDI GCAATG 1 cut(s) 57
BsrGI TGTACA 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 85, 226
BssMI GATC 2 cut(s) 45, 103
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 3 cut(s) 50, 297, 566
BstF5I GGATG 2 cut(s) 81, 493
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 4 cut(s) 56, 64, 412, 469
BstKTI GATC 2 cut(s) 48, 106
BstMBI GATC 2 cut(s) 45, 103
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 2 cut(s) 132, 192
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 102
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 2 cut(s) 81, 493
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 443
CfoI GCGC 4 cut(s) 56, 64, 412, 469
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 2 cut(s) 237, 472
CviJI RGCY 7 cut(s) 130, 197, 225, 434, 443, 464, 628
CviKI_1 RGCY 7 cut(s) 130, 197, 225, 434, 443, 464, 628
CviQI GTAC 2 cut(s) 237, 472
DdeI CTNAG 3 cut(s) 50, 297, 566
DpnI GATC 2 cut(s) 47, 105
DpnII GATC 2 cut(s) 45, 103
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 85
FauNDI CATATG 1 cut(s) 598
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 2 cut(s) 68, 500
Fsp4HI GCNGC 1 cut(s) 465
FspI TGCGCA 2 cut(s) 411, 468
GlaI GCGC 4 cut(s) 55, 63, 411, 468
GluI GCNGC 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 1 cut(s) 225
HhaI GCGC 4 cut(s) 56, 64, 412, 469
Hin6I GCGC 4 cut(s) 54, 62, 410, 467
HinP1I GCGC 4 cut(s) 54, 62, 410, 467
HinfI GANTC 1 cut(s) 109
HphI GGTGA 2 cut(s) 271, 316
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 6 cut(s) 36, 108, 179, 373, 382, 400
Hpy188III TCNNGA 4 cut(s) 49, 357, 438, 543
Hpy8I GTNNAC 1 cut(s) 237
HpyAV CCTTC 3 cut(s) 19, 341, 571
HpyCH4IV ACGT 4 cut(s) 71, 183, 276, 530
HpyCH4V TGCA 3 cut(s) 134, 212, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 3 cut(s) 50, 297, 566
HpySE526I ACGT 4 cut(s) 71, 183, 276, 530
HspAI GCGC 4 cut(s) 54, 62, 410, 467
Kzo9I GATC 2 cut(s) 45, 103
LmnI GCTCC 1 cut(s) 440
LpnPI CCDG 9 cut(s) 62, 120, 144, 212, 239, 398, 423, 435, 528
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 2 cut(s) 99, 548
MaeII ACGT 4 cut(s) 71, 183, 276, 530
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 47, 105
MboI GATC 2 cut(s) 45, 103
MboII GAAGA 1 cut(s) 585
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 4 cut(s) 240, 424, 492, 496
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 5 cut(s) 36, 292, 295, 388, 575
MseI TTAA 2 cut(s) 476, 495
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 440
NdeI CATATG 1 cut(s) 598
NdeII GATC 2 cut(s) 45, 103
NlaIV GGNNCC 2 cut(s) 47, 176
NmuCI GTSAC 1 cut(s) 277
NsbI TGCGCA 2 cut(s) 411, 468
PaeR7I CTCGAG 1 cut(s) 357
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 1 cut(s) 466
PshBI ATTAAT 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 47, 176
PstI CTGCAG 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 2 cut(s) 238, 473
RsaNI GTAC 2 cut(s) 237, 472
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 476, 495
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 2 cut(s) 45, 103
SbfI CCTGCAGG 1 cut(s) 136
ScaI AGTACT 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 227
SdaI CCTGCAGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 2 cut(s) 99, 548
SfcI CTRYAG 2 cut(s) 132, 192
Sfr274I CTCGAG 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse8387I CCTGCAGG 1 cut(s) 136
Sse9I AATT 4 cut(s) 240, 424, 492, 496
SsiI CCGC 2 cut(s) 150, 514
SspI AATATT 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 85
TaiI ACGT 4 cut(s) 74, 186, 279, 533
TaqI TCGA 3 cut(s) 102, 334, 358
TasI AATT 4 cut(s) 240, 424, 492, 496
TatI WGTACW 2 cut(s) 236, 471
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 476, 495
Tru9I TTAA 2 cut(s) 476, 495
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 2 cut(s) 504, 585
TspGWI ACGGA 1 cut(s) 138
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 357
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.