RLG00000032276

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018814
Physical Location & Seq
Reverse (-)
12983677 .. 12984401
725 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032276

Sequence Viewer

Length: 645 bp
ATGGCCTCTCGATTTCTGTTGCTAGCATTACTCGTACTCATGACTTGCTCCATTGCTTTGGCAACTGAGAGTCTTCAAGATTTCTGCGTAGCACAGCCAACACGCTTAGCTGCAGTGAATGGCGTTGTCTGCAAGGACCCTAATCTTGTTGAAGCCAATGACTTCTACTATAGTGGACTTGACATAGCAGGCAACACATCAACTGCATTCGGTTCACGTGTGACCTCTGTTAGGGTGAACCAAATTCCAGGACTCAATACCTTTGGCATCTCCATTGCACGCATCGATTTTGCTGCATGGGGTGTTAATCCTCCCCATACACACCCTCGTGCTGCCGAAATCTTGACAGTCCTTGAAGGCAGCCTCCAAGTTGGTTTTGTCACCTCCAACCCCGATAACCGTCTCATCACAAAAATACTTCAGGCAGGTGATGTGTTTGTGTTCCCGGAAGGTCTTGTGCATTTCCAATACAATGTTGGATATACTACTGCAGTCGCCTTTTCAGCTTTCAGCAGCCAAAATCCAGGAACTATTACCCTTGCCAACACAGTATTTGGATCAAGGCCTCACATCTCTAGTGACATTCTTACAAAGGCTTTCCTAGTGGATAAGCAAGCAATCTACAACATACAGGCAAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

215

Amino Acids

23.04

Weight (kDa)

6.4

Isoelectric Point (pI)

26.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 59 - 205 5.1e-49 Cupin
Cupin_2 PF07883 101 - 167 4e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018814)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10640
rosa_laevigata RLG00000032276
rosa_multiflora Rmu_co8267887.1_g000001 Rmu_sc0005319.1_g000007
rosa_rugosa Rorug05G0034200
rosa_samantha Rh5AG129300 Rh5BG127100 Rh5CG139900
rosa_wichuraiana Rw5G011170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 416
AclWI GGATC 1 cut(s) 565
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 404
AcvI CACGTG 1 cut(s) 218
AfaI GTAC 1 cut(s) 36
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 3 cut(s) 77, 152, 356
AjnI CCWGG 2 cut(s) 247, 523
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 2 cut(s) 110, 506
AluI AGCT 2 cut(s) 110, 506
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 1 cut(s) 565
AoxI GGCC 2 cut(s) 3, 563
ApeKI GCWGC 5 cut(s) 110, 293, 332, 360, 513
ApoI RAATTY 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 247, 373, 440
AsuNHI GCTAGC 1 cut(s) 22
AvaII GGWCC 1 cut(s) 136
BauI CACGAG 1 cut(s) 327
BbrPI CACGTG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 65
BbvI GCAGC 5 cut(s) 97, 280, 319, 372, 525
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 2 cut(s) 249, 525
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 3 cut(s) 23, 576, 602
BfmI CTRYAG 3 cut(s) 111, 169, 489
BfuAI ACCTGC 1 cut(s) 416
BisI GCNGC 5 cut(s) 111, 294, 333, 361, 514
BlpI GCTNAGC 1 cut(s) 106
BlsI GCNGC 5 cut(s) 112, 295, 334, 362, 515
Bme1390I CCNGG 3 cut(s) 249, 446, 525
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 3 cut(s) 249, 446, 525
BmsI GCATC 2 cut(s) 276, 291
BmtI GCTAGC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 65
Bpu1102I GCTNAGC 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 446
Bsa29I ATCGAT 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse3DI GCAATG 2 cut(s) 51, 273
BseBI CCWGG 2 cut(s) 249, 525
BseCI ATCGAT 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 2 cut(s) 51, 273
BseMII CTCAG 1 cut(s) 57
BseXI GCAGC 5 cut(s) 97, 280, 319, 372, 525
BshFI GGCC 2 cut(s) 5, 565
BshVI ATCGAT 1 cut(s) 285
BsiSI CCGG 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 407
BsmBI CGTCTC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 2 cut(s) 5, 565
Bsp143I GATC 1 cut(s) 557
Bsp1720I GCTNAGC 1 cut(s) 106
BspANI GGCC 2 cut(s) 5, 565
BspCNI CTCAG 1 cut(s) 58
BspDI ATCGAT 1 cut(s) 285
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 138
BspMAI CTGCAG 2 cut(s) 115, 493
BspMI ACCTGC 1 cut(s) 416
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 565
BsrDI GCAATG 2 cut(s) 51, 273
BssMI GATC 1 cut(s) 557
BssSI CACGAG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 249, 525
Bst4CI ACNGT 3 cut(s) 349, 401, 550
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 24, 190, 280, 615
BstDEI CTNAG 2 cut(s) 66, 106
BstENI CCTNNNNNAGG 1 cut(s) 229
BstKTI GATC 1 cut(s) 560
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 1 cut(s) 557
BstMWI GCNNNNNNNGC 2 cut(s) 129, 503
BstNI CCWGG 2 cut(s) 249, 525
BstSCI CCNGG 3 cut(s) 247, 444, 523
BstSFI CTRYAG 3 cut(s) 111, 169, 489
BstV1I GCAGC 5 cut(s) 97, 280, 319, 372, 525
BstV2I GAAGAC 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 2 cut(s) 5, 565
BsuTUI ATCGAT 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 416
Cac8I GCNNGC 4 cut(s) 24, 190, 280, 615
CciI TCATGA 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 285
Csp6I GTAC 1 cut(s) 35
CviAII CATG 2 cut(s) 40, 297
CviJI RGCY 9 cut(s) 5, 97, 110, 155, 363, 506, 516, 565, 596
CviKI_1 RGCY 9 cut(s) 5, 97, 110, 155, 363, 506, 516, 565, 596
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 2 cut(s) 66, 106
DpnI GATC 1 cut(s) 559
DpnII GATC 1 cut(s) 557
Eco147I AGGCCT 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 404
Eco72I CACGTG 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 247, 523
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 2 cut(s) 43, 300
FaiI YATR 7 cut(s) 41, 171, 185, 298, 318, 483, 629
FatI CATG 2 cut(s) 39, 296
Fnu4HI GCNGC 5 cut(s) 111, 294, 333, 361, 514
Fsp4HI GCNGC 5 cut(s) 111, 294, 333, 361, 514
FspBI CTAG 3 cut(s) 23, 576, 602
GluI GCNGC 5 cut(s) 111, 294, 333, 361, 514
HaeIII GGCC 2 cut(s) 5, 565
HapII CCGG 1 cut(s) 446
Hin1II CATG 2 cut(s) 43, 300
HinfI GANTC 2 cut(s) 70, 252
HpaII CCGG 1 cut(s) 446
HphI GGTGA 3 cut(s) 247, 373, 440
Hpy166II GTNNAC 3 cut(s) 176, 215, 238
Hpy188III TCNNGA 4 cut(s) 9, 40, 77, 343
Hpy8I GTNNAC 3 cut(s) 176, 215, 238
HpyAV CCTTC 2 cut(s) 350, 443
HpyCH4III ACNGT 3 cut(s) 349, 401, 550
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 7 cut(s) 113, 132, 206, 278, 296, 460, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 503
HpyF3I CTNAG 2 cut(s) 66, 106
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 2 cut(s) 43, 300
Kzo9I GATC 1 cut(s) 557
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 9 cut(s) 174, 234, 261, 407, 411, 459, 510, 537, 617
Lsp1109I GCAGC 5 cut(s) 97, 280, 319, 372, 525
LweI GCATC 2 cut(s) 276, 291
MaeI CTAG 3 cut(s) 23, 576, 602
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 3 cut(s) 220, 379, 578
MalI GATC 1 cut(s) 559
MboI GATC 1 cut(s) 557
MboII GAAGA 1 cut(s) 65
MluCI AATT 1 cut(s) 243
MlyI GAGTC 2 cut(s) 79, 246
MmeI TCCRAC 2 cut(s) 411, 457
MnlI CCTC 7 cut(s) 16, 235, 321, 336, 374, 394, 576
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 1 cut(s) 446
MspR9I CCNGG 3 cut(s) 249, 446, 525
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 2 cut(s) 249, 525
MwoI GCNNNNNNNGC 2 cut(s) 129, 503
NciI CCSGG 1 cut(s) 446
NdeII GATC 1 cut(s) 557
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 2 cut(s) 43, 300
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 3 cut(s) 220, 379, 578
OliI CACNNNNGTG 1 cut(s) 327
PagI TCATGA 1 cut(s) 39
PaqCI CACCTGC 1 cut(s) 416
PceI AGGCCT 1 cut(s) 565
PctI GAATGC 1 cut(s) 206
PfoI TCCNGGA 3 cut(s) 247, 444, 523
PkrI GCNGC 5 cut(s) 112, 295, 334, 362, 515
PleI GAGTC 2 cut(s) 78, 246
PmaCI CACGTG 1 cut(s) 218
PmlI CACGTG 1 cut(s) 218
PpsI GAGTC 2 cut(s) 78, 246
Ppu21I YACGTR 1 cut(s) 218
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 247, 523
PspCI CACGTG 1 cut(s) 218
PspGI CCWGG 2 cut(s) 247, 523
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 2 cut(s) 115, 493
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 5 cut(s) 111, 294, 333, 361, 514
Sau3AI GATC 1 cut(s) 557
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 2 cut(s) 79, 246
ScrFI CCNGG 3 cut(s) 249, 446, 525
SetI ASST 9 cut(s) 112, 220, 227, 263, 386, 430, 454, 508, 641
SfaNI GCATC 2 cut(s) 276, 291
SfcI CTRYAG 3 cut(s) 111, 169, 489
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 1 cut(s) 243
SseBI AGGCCT 1 cut(s) 565
SspMI CTAG 3 cut(s) 23, 576, 602
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 3 cut(s) 247, 444, 523
TaaI ACNGT 3 cut(s) 349, 401, 550
TaiI ACGT 1 cut(s) 220
TaqI TCGA 2 cut(s) 10, 285
TasI AATT 1 cut(s) 243
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 3 cut(s) 220, 379, 578
TseI GCWGC 5 cut(s) 110, 293, 332, 360, 513
Tsp45I GTSAC 3 cut(s) 220, 379, 578
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 229
XapI RAATTY 1 cut(s) 243
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XspI CTAG 3 cut(s) 23, 576, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.