Rorug05G0034200

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018814
Physical Location & Seq
Forward (+)
2358118 .. 2359771
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0034200.1

Sequence Viewer

Length: 984 bp
ATGAATTCTTCAACATCATTGCTTTTCATTTTTGCCACTATTATCGTTGCTCCGGCCATAGTTGACGCTCAATGCACTACCAATGCTGACTACTGCTGGAGCTGTAATACTACATCTAATGCCACCACCACCTACAAACAAAACCTCAACACACTCCTCGTCTCTTTGTCTACCAACAACCAAACCAACTACGGGTTCCTCAATTTGTCTCTGGGAACTGGCACCAACAAAGTGAATGTAATTGCACTCTGTAGAGGAGACCTTGCCCAAGACCAGTGTCGTAGTTGTTACGGCGAAGCCAACATCATTCTCTCTCAGAATTGCTCTAATCATCACGAAGCAATCATATGGGCCCTGCGTTGCACTGTTCGATACTCGAACATCACCATGTTCGGAATCGAAGAAGACGAGCCCGCTCAGTTGTTGCCGAGCCCTAACAGTGCGTCAAACCCTGAAGATTTCAAGCTGGTGCTTAATCCCTTGTTGGAAACTTTGAGCCGAAATGCATCATCGGGGGATTCGATGAAGAAATTTGCAGCAGGACATGCGCCTGTCCCTGCAGGAAGTGAAACAAATCAAACCATATATGCAAGTGCTCAGTGCAGTCCTGATCTGGACAAGCAAAACTGCAGTGAGTGCCTTGAAGATTCTATTAAGGATATTCCTAAATGTTGTGAAGGAAAGGATGGAGGGAGGGTTCTTAAACCCAGCTGTACTCTGAGGTTTGAGAGAGGAATGTTCTATGAGCCTTCAGCTGATTCGCTAATAAAAATTTCTACAGCCACTCCGCCCCCTACATCCACTCCACCACCTACAGCCAGTCCGCCTACTTCAGCCACTCCGCCCCCTACAGCCAATCCGTCACCTACAGCCAATCCGCCACCTACAGCCACTCCGCCCACTACAGCCACAGAAGGAAATAATACAAGTAACATAACGATAAAAGACGAGAAGGAAGGGAAGGGCCAAACTTGGAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

327

Amino Acids

34.95

Weight (kDa)

5.07

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 35 - 127 2.7e-19 Salt stress response/antifungal
Stress-antifung PF01657 149 - 243 8e-13 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018814)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10640
rosa_laevigata RLG00000032276
rosa_multiflora Rmu_co8267887.1_g000001 Rmu_sc0005319.1_g000007
rosa_rugosa Rorug05G0034200
rosa_samantha Rh5AG129300 Rh5BG127100 Rh5CG139900
rosa_wichuraiana Rw5G011170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 416
AccI GTMKAC 1 cut(s) 170
AciI CCGC 6 cut(s) 414, 788, 824, 842, 878, 896
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 3 cut(s) 4, 530, 771
AcuI CTGAAG 3 cut(s) 474, 735, 816
AfaI GTAC 1 cut(s) 715
AfiI CCNNNNNNNGG 1 cut(s) 192
AgsI TTSAA 3 cut(s) 12, 463, 644
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AloI GAACNNNNNNTCC 2 cut(s) 681, 713
AluBI AGCT 4 cut(s) 102, 466, 711, 755
AluI AGCT 4 cut(s) 102, 466, 711, 755
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 3 cut(s) 166, 213, 252
AoxI GGCC 3 cut(s) 54, 351, 964
ApaI GGGCCC 1 cut(s) 355
ApeKI GCWGC 1 cut(s) 536
ApoI RAATTY 3 cut(s) 4, 530, 771
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 3 cut(s) 351, 352, 964
AsuHPI GGTGA 2 cut(s) 376, 855
BaeGI GKGCMC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 3 cut(s) 355, 414, 434
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 1 cut(s) 548
BccI CCATC 1 cut(s) 680
BceAI ACGGC 1 cut(s) 307
BcoDI GTCTC 3 cut(s) 166, 213, 252
BfmI CTRYAG 9 cut(s) 250, 558, 628, 777, 813, 849, 867, 885, 903
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
BmgT120I GGNCC 3 cut(s) 351, 352, 964
BmiI GGNNCC 3 cut(s) 197, 223, 353
BmsI GCATC 1 cut(s) 515
BoxI GACNNNNGTC 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 118
BsaI GGTCTC 1 cut(s) 252
BsaXI ACNNNNNCTCC 8 cut(s) 681, 711, 769, 787, 799, 817, 877, 907
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 3 cut(s) 223, 274, 819
Bse3DI GCAATG 1 cut(s) 17
BseGI GGATG 2 cut(s) 691, 797
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMI GCAATG 1 cut(s) 17
BseMII CTCAG 4 cut(s) 329, 431, 611, 710
BseNI ACTGG 3 cut(s) 223, 274, 819
BseRI GAGGAG 2 cut(s) 146, 270
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 1 cut(s) 548
BseYI CCCAGC 1 cut(s) 707
BsgI GTGCAG 1 cut(s) 622
BshFI GGCC 3 cut(s) 56, 353, 966
BshNI GGYRCC 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 3 cut(s) 166, 213, 252
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 539
BsnI GGCC 3 cut(s) 56, 353, 966
Bso31I GGTCTC 1 cut(s) 252
Bsp120I GGGCCC 1 cut(s) 351
Bsp1286I GDGCHC 4 cut(s) 355, 414, 434, 598
Bsp143I GATC 1 cut(s) 610
BspACI CCGC 6 cut(s) 414, 788, 824, 842, 878, 896
BspANI GGCC 3 cut(s) 56, 353, 966
BspCNI CTCAG 4 cut(s) 328, 430, 610, 711
BspLI GGNNCC 3 cut(s) 197, 223, 353
BspMAI CTGCAG 2 cut(s) 562, 632
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 252
BsrBI CCGCTC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 17
BsrI ACTGG 3 cut(s) 223, 274, 819
BssMI GATC 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 367, 440
BstAPI GCANNNNNTGC 2 cut(s) 545, 636
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 4 cut(s) 315, 417, 597, 719
BstF5I GGATG 2 cut(s) 691, 797
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 1 cut(s) 613
BstMAI GTCTC 3 cut(s) 166, 213, 252
BstMBI GATC 1 cut(s) 610
BstMWI GCNNNNNNNGC 2 cut(s) 545, 636
BstNSI RCATGY 1 cut(s) 548
BstPAI GACNNNNGTC 1 cut(s) 276
BstSFI CTRYAG 9 cut(s) 250, 558, 628, 777, 813, 849, 867, 885, 903
BstSLI GKGCMC 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 548
BstV2I GAAGAC 1 cut(s) 411
BsuRI GGCC 3 cut(s) 56, 353, 966
BtsCI GGATG 2 cut(s) 691, 797
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 5 cut(s) 281, 363, 445, 605, 637
Cac8I GCNNGC 1 cut(s) 414
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 3 cut(s) 351, 352, 964
CseI GACGC 2 cut(s) 74, 432
Csp6I GTAC 1 cut(s) 714
CviAII CATG 2 cut(s) 388, 545
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 4 cut(s) 315, 417, 597, 719
DpnI GATC 1 cut(s) 612
DpnII GATC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 54
EciI GGCGGA 5 cut(s) 777, 813, 831, 867, 885
Eco24I GRGCYC 3 cut(s) 355, 414, 434
Eco31I GGTCTC 1 cut(s) 252
Eco57I CTGAAG 3 cut(s) 474, 735, 816
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 4
EcoT22I ATGCAT 1 cut(s) 508
EcoT38I GRGCYC 3 cut(s) 355, 414, 434
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 2 cut(s) 391, 548
FaqI GGGAC 1 cut(s) 539
FatI CATG 2 cut(s) 387, 544
FauI CCCGC 1 cut(s) 421
FauNDI CATATG 1 cut(s) 347
FblI GTMKAC 1 cut(s) 170
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 2 cut(s) 698, 784
FriOI GRGCYC 3 cut(s) 355, 414, 434
Fsp4HI GCNGC 1 cut(s) 537
GlaI GCGC 1 cut(s) 549
GluI GCNGC 1 cut(s) 537
GsaI CCCAGC 1 cut(s) 711
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 3 cut(s) 56, 353, 966
HapII CCGG 1 cut(s) 53
HgaI GACGC 2 cut(s) 74, 432
HhaI GCGC 1 cut(s) 550
Hin1II CATG 2 cut(s) 391, 548
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 4 cut(s) 396, 518, 647, 758
HpaII CCGG 1 cut(s) 53
HphI GGTGA 2 cut(s) 376, 855
Hpy166II GTNNAC 2 cut(s) 64, 171
Hpy188I TCNGA 3 cut(s) 318, 395, 720
Hpy188III TCNNGA 3 cut(s) 335, 608, 614
Hpy8I GTNNAC 2 cut(s) 64, 171
HpyAV CCTTC 6 cut(s) 671, 759, 908, 946, 950, 955
HpyCH4III ACNGT 2 cut(s) 367, 440
HpyCH4V TGCA 9 cut(s) 75, 245, 363, 506, 536, 560, 590, 603, 630
HpyF10VI GCNNNNNNNGC 2 cut(s) 545, 636
HpyF3I CTNAG 4 cut(s) 315, 417, 597, 719
Hsp92II CATG 2 cut(s) 391, 548
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 1 cut(s) 610
LmnI GCTCC 2 cut(s) 55, 99
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 1 cut(s) 515
MaeIII GTNAC 3 cut(s) 287, 861, 929
MalI GATC 1 cut(s) 612
MbiI CCGCTC 1 cut(s) 416
MboI GATC 1 cut(s) 610
MboII GAAGA 5 cut(s) 413, 416, 467, 538, 656
MhlI GDGCHC 4 cut(s) 355, 414, 434, 598
MluCI AATT 6 cut(s) 4, 202, 240, 319, 530, 771
MmeI TCCRAC 1 cut(s) 465
MnlI CCTC 8 cut(s) 155, 167, 209, 248, 683, 687, 714, 725
Mph1103I ATGCAT 1 cut(s) 508
MseI TTAA 4 cut(s) 474, 654, 702, 982
MslI CAYNNNNRTG 1 cut(s) 386
MspA1I CMGCKG 2 cut(s) 711, 755
MspI CCGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 545, 636
NdeI CATATG 1 cut(s) 347
NdeII GATC 1 cut(s) 610
NlaIII CATG 2 cut(s) 391, 548
NlaIV GGNNCC 3 cut(s) 197, 223, 353
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 1 cut(s) 861
NsiI ATGCAT 1 cut(s) 508
NspI RCATGY 1 cut(s) 548
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 518
PfeI GAWTC 4 cut(s) 396, 518, 647, 758
PkrI GCNGC 1 cut(s) 538
PshAI GACNNNNGTC 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 707
PspN4I GGNNCC 3 cut(s) 197, 223, 353
PspOMI GGGCCC 1 cut(s) 351
PspPI GGNCC 3 cut(s) 351, 352, 964
PstI CTGCAG 2 cut(s) 562, 632
PvuII CAGCTG 2 cut(s) 711, 755
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 4 cut(s) 474, 654, 702, 982
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 1 cut(s) 610
Sau96I GGNCC 3 cut(s) 351, 352, 964
SbfI CCTGCAGG 1 cut(s) 562
SdaI CCTGCAGG 1 cut(s) 562
SduI GDGCHC 4 cut(s) 355, 414, 434, 598
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 9 cut(s) 250, 558, 628, 777, 813, 849, 867, 885, 903
SmiMI CAYNNNNRTG 1 cut(s) 386
Sse8387I CCTGCAGG 1 cut(s) 562
Sse9I AATT 6 cut(s) 4, 202, 240, 319, 530, 771
SsiI CCGC 6 cut(s) 414, 788, 824, 842, 878, 896
TaaI ACNGT 2 cut(s) 367, 440
TaqI TCGA 4 cut(s) 370, 377, 399, 521
TasI AATT 6 cut(s) 4, 202, 240, 319, 530, 771
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 4 cut(s) 396, 518, 647, 758
Tru1I TTAA 4 cut(s) 474, 654, 702, 982
Tru9I TTAA 4 cut(s) 474, 654, 702, 982
TscAI CASTG 5 cut(s) 281, 370, 445, 605, 637
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 861
TspDTI ATGAA 3 cut(s) 16, 17, 539
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 5 cut(s) 281, 370, 445, 605, 637
XapI RAATTY 3 cut(s) 4, 530, 771
XceI RCATGY 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.