Rmu_sc0005319.1_g000007

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005319.1
OG0018814
Physical Location & Seq
Forward (+)
20153 .. 20838
686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005319.1_g000007.1.cds

Sequence Viewer

Length: 606 bp
atgacttgctccattgctttggcaactgagagtcttcaagatttctgcgtagcacagccaacacgcttagctgcagtgaatggcgttgtctgcaaggaccctaatcttgttgaagccaatgacttcttctatagtggactcgacataacaggcaacacatcaactgcattcggttcacgtgtgacctctgttagggtgaaccaaattccaggactcaacacgtttggcatctccattgcacgcatcgattttgctccatggggtgttaaccctccccacacacaccctcgcgctgccgaaatcttgacagtccttgaaggcagcctccaagttggttttgtcacctccaaccccgataatcgtctcatcacaaaaatacttcaggctggtgatgtgtttgtgttcccggaaggtcttgtgcatttccaatacaatgttggatatactactgcagtcgccttttcagcttttagcagccaaaacccaggaactgttacccttgccaacacggtatttggatcaaggcctcacatctctagtgacattcttacaaaggctttcctagtggataagcaagcaatctacaacatacaggcaaggttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

201

Amino Acids

21.63

Weight (kDa)

6.03

Isoelectric Point (pI)

25.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018814)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10640
rosa_laevigata RLG00000032276
rosa_multiflora Rmu_co8267887.1_g000001 Rmu_sc0005319.1_g000007
rosa_rugosa Rorug05G0034200
rosa_samantha Rh5AG129300 Rh5BG127100 Rh5CG139900
rosa_wichuraiana Rw5G011170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 291
AclWI GGATC 1 cut(s) 526
AcsI RAATTY 1 cut(s) 204
AcuI CTGAAG 1 cut(s) 365
AcvI CACGTG 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 192
AflIII ACRYGT 2 cut(s) 178, 219
AgsI TTSAA 3 cut(s) 38, 113, 317
AjnI CCWGG 2 cut(s) 208, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 2 cut(s) 71, 467
AluI AGCT 2 cut(s) 71, 467
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 526
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 1 cut(s) 524
ApeKI GCWGC 4 cut(s) 71, 293, 321, 474
ApoI RAATTY 1 cut(s) 204
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 97
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 3 cut(s) 208, 334, 401
AvaII GGWCC 1 cut(s) 97
BbrPI CACGTG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 4 cut(s) 58, 280, 333, 486
BciT130I CCWGG 2 cut(s) 210, 486
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 368
BfaI CTAG 2 cut(s) 537, 563
BfmI CTRYAG 3 cut(s) 72, 130, 450
BisI GCNGC 4 cut(s) 72, 294, 322, 475
BlpI GCTNAGC 1 cut(s) 67
BlsI GCNGC 4 cut(s) 73, 295, 323, 476
Bme1390I CCNGG 3 cut(s) 210, 407, 486
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 3 cut(s) 210, 407, 486
BmsI GCATC 2 cut(s) 237, 252
BpiI GAAGAC 1 cut(s) 26
Bpu1102I GCTNAGC 1 cut(s) 67
BpuMI CCSGG 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 257, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse3DI GCAATG 2 cut(s) 12, 234
BseBI CCWGG 2 cut(s) 210, 486
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 257, 484
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMI GCAATG 2 cut(s) 12, 234
BseMII CTCAG 1 cut(s) 18
BseXI GCAGC 4 cut(s) 58, 280, 333, 486
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 1 cut(s) 526
BshVI ATCGAT 1 cut(s) 246
BsiSI CCGG 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 1 cut(s) 368
BsmBI CGTCTC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 1 cut(s) 526
Bsp143I GATC 1 cut(s) 518
Bsp1720I GCTNAGC 1 cut(s) 67
Bsp19I CCATGG 1 cut(s) 257
BspANI GGCC 1 cut(s) 526
BspCNI CTCAG 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 99
BspMAI CTGCAG 2 cut(s) 76, 454
BspPI GGATC 1 cut(s) 526
BsrDI GCAATG 2 cut(s) 12, 234
BssECI CCNNGG 2 cut(s) 257, 484
BssMI GATC 1 cut(s) 518
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 2 cut(s) 210, 486
Bst4CI ACNGT 3 cut(s) 310, 493, 511
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 241, 576
BstDEI CTNAG 2 cut(s) 27, 67
BstDSI CCRYGG 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 190
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 1 cut(s) 521
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 1 cut(s) 518
BstMWI GCNNNNNNNGC 2 cut(s) 90, 464
BstNI CCWGG 2 cut(s) 210, 486
BstSCI CCNGG 3 cut(s) 208, 405, 484
BstSFI CTRYAG 3 cut(s) 72, 130, 450
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 58, 280, 333, 486
BstV2I GAAGAC 1 cut(s) 26
BstXI CCANNNNNNTGG 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 1 cut(s) 526
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 257
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 2 cut(s) 241, 576
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 97
ClaI ATCGAT 1 cut(s) 246
CviAII CATG 1 cut(s) 258
CviJI RGCY 9 cut(s) 58, 71, 116, 324, 386, 467, 477, 526, 557
CviKI_1 RGCY 9 cut(s) 58, 71, 116, 324, 386, 467, 477, 526, 557
DdeI CTNAG 2 cut(s) 27, 67
DpnI GATC 1 cut(s) 520
DpnII GATC 1 cut(s) 518
Eco130I CCWWGG 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 365
Eco72I CACGTG 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 208, 484
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 1 cut(s) 261
FaiI YATR 5 cut(s) 132, 146, 259, 444, 590
FatI CATG 1 cut(s) 257
Fnu4HI GCNGC 4 cut(s) 72, 294, 322, 475
Fsp4HI GCNGC 4 cut(s) 72, 294, 322, 475
FspBI CTAG 2 cut(s) 537, 563
GlaI GCGC 1 cut(s) 292
GluI GCNGC 4 cut(s) 72, 294, 322, 475
HaeIII GGCC 1 cut(s) 526
HapII CCGG 1 cut(s) 407
HhaI GCGC 1 cut(s) 293
Hin1II CATG 1 cut(s) 261
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 3 cut(s) 31, 138, 213
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 1 cut(s) 407
HphI GGTGA 3 cut(s) 208, 334, 401
Hpy166II GTNNAC 4 cut(s) 137, 176, 199, 268
Hpy188III TCNNGA 2 cut(s) 38, 304
Hpy8I GTNNAC 4 cut(s) 137, 176, 199, 268
HpyAV CCTTC 2 cut(s) 311, 404
HpyCH4III ACNGT 3 cut(s) 310, 493, 511
HpyCH4IV ACGT 2 cut(s) 178, 221
HpyCH4V TGCA 6 cut(s) 74, 93, 167, 239, 421, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 464
HpyF3I CTNAG 2 cut(s) 27, 67
HpySE526I ACGT 2 cut(s) 178, 221
Hsp92II CATG 1 cut(s) 261
HspAI GCGC 1 cut(s) 291
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 1 cut(s) 518
LmnI GCTCC 2 cut(s) 14, 259
LpnPI CCDG 9 cut(s) 135, 195, 222, 368, 372, 420, 471, 498, 578
Lsp1109I GCAGC 4 cut(s) 58, 280, 333, 486
LweI GCATC 2 cut(s) 237, 252
MaeI CTAG 2 cut(s) 537, 563
MaeII ACGT 2 cut(s) 178, 221
MaeIII GTNAC 4 cut(s) 181, 340, 493, 539
MalI GATC 1 cut(s) 520
MboI GATC 1 cut(s) 518
MboII GAAGA 2 cut(s) 26, 118
MluCI AATT 1 cut(s) 204
MlyI GAGTC 3 cut(s) 40, 132, 207
MmeI TCCRAC 2 cut(s) 372, 418
MnlI CCTC 6 cut(s) 196, 282, 297, 335, 355, 537
MseI TTAA 1 cut(s) 267
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 3 cut(s) 210, 407, 486
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 2 cut(s) 210, 486
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 90, 464
NciI CCSGG 1 cut(s) 407
NcoI CCATGG 1 cut(s) 257
NdeII GATC 1 cut(s) 518
NlaIII CATG 1 cut(s) 261
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 3 cut(s) 181, 340, 539
PceI AGGCCT 1 cut(s) 526
PctI GAATGC 1 cut(s) 167
PfoI TCCNGGA 2 cut(s) 208, 405
PkrI GCNGC 4 cut(s) 73, 295, 323, 476
PleI GAGTC 3 cut(s) 39, 132, 207
PmaCI CACGTG 1 cut(s) 179
PmlI CACGTG 1 cut(s) 179
PpsI GAGTC 3 cut(s) 39, 132, 207
Ppu21I YACGTR 1 cut(s) 179
PpuMI RGGWCCY 1 cut(s) 97
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 208, 484
PspCI CACGTG 1 cut(s) 179
PspGI CCWGG 2 cut(s) 208, 484
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 97
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 2 cut(s) 76, 454
PstNI CAGNNNCTG 1 cut(s) 491
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 4 cut(s) 72, 294, 322, 475
Sau3AI GATC 1 cut(s) 518
Sau96I GGNCC 1 cut(s) 97
SchI GAGTC 3 cut(s) 40, 132, 207
ScrFI CCNGG 3 cut(s) 210, 407, 486
SetI ASST 8 cut(s) 73, 181, 188, 224, 347, 415, 469, 602
SfaNI GCATC 2 cut(s) 237, 252
SfcI CTRYAG 3 cut(s) 72, 130, 450
SinI GGWCC 1 cut(s) 97
Sse9I AATT 1 cut(s) 204
SseBI AGGCCT 1 cut(s) 526
SspMI CTAG 2 cut(s) 537, 563
StuI AGGCCT 1 cut(s) 526
StyD4I CCNGG 3 cut(s) 208, 405, 484
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 3 cut(s) 310, 493, 511
TaiI ACGT 2 cut(s) 181, 224
TaqI TCGA 2 cut(s) 141, 246
TasI AATT 1 cut(s) 204
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 3 cut(s) 181, 340, 539
TseI GCWGC 4 cut(s) 71, 293, 321, 474
Tsp45I GTSAC 3 cut(s) 181, 340, 539
TspRI CASTG 1 cut(s) 81
VpaK11BI GGWCC 1 cut(s) 97
XagI CCTNNNNNAGG 1 cut(s) 190
XapI RAATTY 1 cut(s) 204
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XspI CTAG 2 cut(s) 537, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.