Rw5G011170

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018814
Physical Location & Seq
Reverse (-)
12608038 .. 12611621
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011170.1

Sequence Viewer

Length: 648 bp
ATGGCCTCGCAGTTTCTATTGTTAGCATGCCTTGCTATGACAGCTTCTATTGCTTTGGCATCTGACCCAAGTTCTCTTCAGGATTTTTGTGTGGCTGATCCATCAAGCTCAGCTGCAGTGAATGGCGTTGTCTGCAAGGACCCTAATCTTGTTGAAGCCAATGACTTCTTCTATAGTGGACTTGACATAACAGGCAACACATCAACTGCATTCGGTTCACGTGTGACCTCTGTTAGGGTGAACCAAATTCCAGGACTCAACACGTTTGGCATCTCCATTGCACGCATCGATTTTGCTCCATGGGGTGTTAACCCTCCCCACACACACCCTCGCGCTGCCGAAATCTTGACAGTCCTTGAAGGCAGCCTCCAAGTTGGTTTTGTCACCTCCAACCCCGATAATCGTCTCATCACAAAAATACTTCAGGCTGGTGATGTGTTTGTGTTCCCGGAAGGTCTTGTGCATTTCCAATACAATGTTGGATATACTACTGCAGTCGCCTTTTCAGCTTTTAGCAGCCAAAACCCAGGAACTGTTACCCTTGCCAACACGGTATTTGGATCAAGGCCTCACATCTCTAGTGACATTCTTACAAAGGCTTTCCTAGTGGATAAGCAAGCAATCTACAACATACAGGCAAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

215

Amino Acids

22.89

Weight (kDa)

5.41

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 60 - 206 2e-49 Cupin
Cupin_2 PF07883 96 - 168 7.2e-09 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018814)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10640
rosa_laevigata RLG00000032276
rosa_multiflora Rmu_co8267887.1_g000001 Rmu_sc0005319.1_g000007
rosa_rugosa Rorug05G0034200
rosa_samantha Rh5AG129300 Rh5BG127100 Rh5CG139900
rosa_wichuraiana Rw5G011170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 333
AclWI GGATC 2 cut(s) 92, 568
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 62, 407
AcvI CACGTG 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 234
AflIII ACRYGT 2 cut(s) 220, 261
AgsI TTSAA 2 cut(s) 155, 359
AjnI CCWGG 2 cut(s) 250, 526
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 4 cut(s) 44, 108, 113, 509
AluI AGCT 4 cut(s) 44, 108, 113, 509
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 2 cut(s) 92, 568
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 2 cut(s) 3, 566
ApeKI GCWGC 4 cut(s) 113, 335, 363, 516
ApoI RAATTY 1 cut(s) 246
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 139
AsuC2I CCSGG 1 cut(s) 449
AsuHPI GGTGA 3 cut(s) 250, 376, 443
AvaII GGWCC 1 cut(s) 139
BbrPI CACGTG 1 cut(s) 221
BbvI GCAGC 4 cut(s) 100, 322, 375, 528
BccI CCATC 1 cut(s) 109
BciT130I CCWGG 2 cut(s) 252, 528
BcnI CCSGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 2 cut(s) 579, 605
BfmI CTRYAG 3 cut(s) 114, 172, 492
BisI GCNGC 4 cut(s) 114, 336, 364, 517
BlpI GCTNAGC 1 cut(s) 109
BlsI GCNGC 4 cut(s) 115, 337, 365, 518
Bme1390I CCNGG 3 cut(s) 252, 449, 528
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 3 cut(s) 252, 449, 528
BmsI GCATC 3 cut(s) 68, 279, 294
Bpu1102I GCTNAGC 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 449
Bsa29I ATCGAT 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 299, 526
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 2 cut(s) 252, 528
BseCI ATCGAT 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 299, 526
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 1 cut(s) 123
BseXI GCAGC 4 cut(s) 100, 322, 375, 528
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 5, 568
BshVI ATCGAT 1 cut(s) 288
BsiSI CCGG 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 1 cut(s) 410
BsmBI CGTCTC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 2 cut(s) 5, 568
Bsp143I GATC 2 cut(s) 97, 560
Bsp1720I GCTNAGC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 299
BspANI GGCC 2 cut(s) 5, 568
BspCNI CTCAG 1 cut(s) 122
BspDI ATCGAT 1 cut(s) 288
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 141
BspMAI CTGCAG 2 cut(s) 118, 496
BspPI GGATC 2 cut(s) 92, 568
BsrDI GCAATG 1 cut(s) 276
BssECI CCNNGG 2 cut(s) 299, 526
BssMI GATC 2 cut(s) 97, 560
BssT1I CCWWGG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 252, 528
Bst4CI ACNGT 3 cut(s) 352, 535, 553
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 28, 283, 618
BstDEI CTNAG 1 cut(s) 109
BstDSI CCRYGG 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 232
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 2 cut(s) 100, 563
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 2 cut(s) 97, 560
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 50, 132, 506
BstNI CCWGG 2 cut(s) 252, 528
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 3 cut(s) 250, 447, 526
BstSFI CTRYAG 3 cut(s) 114, 172, 492
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 100, 322, 375, 528
Bsu15I ATCGAT 1 cut(s) 288
BsuRI GGCC 2 cut(s) 5, 568
BsuTUI ATCGAT 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 299
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 3 cut(s) 28, 283, 618
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 139
ClaI ATCGAT 1 cut(s) 288
CviAII CATG 2 cut(s) 27, 300
DdeI CTNAG 1 cut(s) 109
DpnI GATC 2 cut(s) 99, 562
DpnII GATC 2 cut(s) 97, 560
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 299
Eco147I AGGCCT 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 62, 407
Eco72I CACGTG 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 250, 526
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 2 cut(s) 30, 303
FaiI YATR 7 cut(s) 28, 38, 174, 188, 301, 486, 632
FatI CATG 2 cut(s) 26, 299
Fnu4HI GCNGC 4 cut(s) 114, 336, 364, 517
Fsp4HI GCNGC 4 cut(s) 114, 336, 364, 517
FspBI CTAG 2 cut(s) 579, 605
GlaI GCGC 1 cut(s) 334
GluI GCNGC 4 cut(s) 114, 336, 364, 517
HaeIII GGCC 2 cut(s) 5, 568
HapII CCGG 1 cut(s) 449
HhaI GCGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 30, 303
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 1 cut(s) 255
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 1 cut(s) 449
HphI GGTGA 3 cut(s) 250, 376, 443
Hpy166II GTNNAC 4 cut(s) 179, 218, 241, 310
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 80, 346
Hpy8I GTNNAC 4 cut(s) 179, 218, 241, 310
HpyAV CCTTC 2 cut(s) 353, 446
HpyCH4III ACNGT 3 cut(s) 352, 535, 553
HpyCH4IV ACGT 2 cut(s) 220, 263
HpyCH4V TGCA 6 cut(s) 116, 135, 209, 281, 463, 494
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 50, 132, 506
HpyF3I CTNAG 1 cut(s) 109
HpySE526I ACGT 2 cut(s) 220, 263
Hsp92II CATG 2 cut(s) 30, 303
HspAI GCGC 1 cut(s) 333
KspAI GTTAAC 1 cut(s) 310
Kzo9I GATC 2 cut(s) 97, 560
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 4 cut(s) 100, 322, 375, 528
LweI GCATC 3 cut(s) 68, 279, 294
MaeI CTAG 2 cut(s) 579, 605
MaeII ACGT 2 cut(s) 220, 263
MaeIII GTNAC 4 cut(s) 223, 382, 535, 581
MalI GATC 2 cut(s) 99, 562
MboI GATC 2 cut(s) 97, 560
MboII GAAGA 2 cut(s) 68, 160
MluCI AATT 1 cut(s) 246
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 2 cut(s) 414, 460
MnlI CCTC 7 cut(s) 16, 238, 324, 339, 377, 397, 579
MseI TTAA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 3 cut(s) 252, 449, 528
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 2 cut(s) 252, 528
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 50, 132, 506
NciI CCSGG 1 cut(s) 449
NcoI CCATGG 1 cut(s) 299
NdeII GATC 2 cut(s) 97, 560
NlaIII CATG 2 cut(s) 30, 303
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 3 cut(s) 223, 382, 581
NspI RCATGY 1 cut(s) 30
PaeI GCATGC 1 cut(s) 30
PceI AGGCCT 1 cut(s) 568
PctI GAATGC 1 cut(s) 209
PfoI TCCNGGA 2 cut(s) 250, 447
PkrI GCNGC 4 cut(s) 115, 337, 365, 518
PleI GAGTC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 221
PmlI CACGTG 1 cut(s) 221
PpsI GAGTC 1 cut(s) 249
Ppu21I YACGTR 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 2 cut(s) 250, 526
PspCI CACGTG 1 cut(s) 221
PspGI CCWGG 2 cut(s) 250, 526
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
PstI CTGCAG 2 cut(s) 118, 496
PstNI CAGNNNCTG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 113
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 4 cut(s) 114, 336, 364, 517
Sau3AI GATC 2 cut(s) 97, 560
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 1 cut(s) 249
ScrFI CCNGG 3 cut(s) 252, 449, 528
SfaNI GCATC 3 cut(s) 68, 279, 294
SfcI CTRYAG 3 cut(s) 114, 172, 492
SinI GGWCC 1 cut(s) 139
SphI GCATGC 1 cut(s) 30
Sse9I AATT 1 cut(s) 246
SseBI AGGCCT 1 cut(s) 568
SspMI CTAG 2 cut(s) 579, 605
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 3 cut(s) 250, 447, 526
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 3 cut(s) 352, 535, 553
TaiI ACGT 2 cut(s) 223, 266
TaqI TCGA 1 cut(s) 288
TasI AATT 1 cut(s) 246
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 3 cut(s) 223, 382, 581
TseI GCWGC 4 cut(s) 113, 335, 363, 516
Tsp45I GTSAC 3 cut(s) 223, 382, 581
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 139
XagI CCTNNNNNAGG 1 cut(s) 232
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 30
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 2 cut(s) 579, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.