RLG00000032513

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019425
Physical Location & Seq
Reverse (-)
15992254 .. 15994360
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032513

Sequence Viewer

Length: 1443 bp
ATGCATCTTCCTCTGAAAAAAGTCCCCATTTACACACCAAAATGGCTAGCAGCTTGTGCATCAATGTTTGCTTCCATGATGCTTATTCAGTCCATGACTAACAATTTTTATACCTTGATCCCCCAACCCCTCCAGAGGTTTGTCATTTCCATTGTCATCGGCTTCTTCAAACGAAGTGTACCCCTTTTCATCCAAAGGCTTCTCCTTTCAGCCTTCCATGAGTTCTCCAGACGATTCTTTCATGACCCCATCATGACCTTACTAGTTGAGGAATACCATGGCTTTTGTGACAACGAAATTTACCATGCTGTGGAGCACTACTTGCATAGCAAAATCAGCCCAAATACTCGACTACTTAAAATAGTGCAAACCTGGCGAGACACTAGTTCCTCCTACCAATTTGTTGACAGGCAAGAGTTTGAAGACGTGTATGAGGGCATTGAGCTGAAATGGAAGTTCAGTTCCACTTCAAATCAGCAAAGCTTCCAGCTTATGTTTGACGTAAGGCACAAGGAAGCAGTGGGGTGTTCTTACATTCCTTATGTTTTGAAGAGGTTTAAGGCCATGAAAGAGGAAACAAAGATTGTCAGGCTGTGCACACTTGCGAGGAAAAATGGGCATAACATCAAGTGGCAATCAATAACATTCGGAAACTACTCTGCCACATTTGCTACTCTAGCCTTGGATCCCAAGCTGAAGACATCGATTTTAGAGGATTTGGACCGGTTTGTGAAGGGCAAAGAGTTCTACCAAGGTGTAGGAAGGGCTTGGAAGAGAGGCTATCTTCTATATGGTCCCCCCGGCACTGGTAAATCCTCCCTGATAGCTGCAATTGCTAATCATCTCAAGTTCAACATCTATGACTTGCAGCTCTCACATTTGAAATCCGATGTGGAACTCAGAAGCGTACTAGTTGCAACCACAAACCAATCCATACTTGTGATTGAGGATATAGATTGCGGTCTGAAGCTACAAAATAGAACCCTTGTTAAGACAACCAGCAGTTCGAAAGAGAAAGACCAAGCAGATGAGCCGGCTATTACACTTTCTGGCCTACTGAACTTCATAGATGGCCTATGGTCTGGTTCAGCAGAGCAAAGGATAATCATATTCACTACCAATCACAAGGACAGGCTTGACCCTGCATTGCTGCGTCCCGGACGAATGGATATGCATATCCACATGTCATATTGCACCTTTGGTGGGTTCGAAACCTTGGCGCGCAATTACTTGGGGATCAAAGAACACCAGCCTTTGTTTGATGAGATTGAAAGCCTGCTGCAAAATGCAGAGGTCACTCCTGCACAAGTAGCGCAAGAGTTGATGAAGAGTGACGATGCTGAAACTGCACTCCAAGGTCTGATCACAATGCTGAGGAAGAAGATCGAACATCAAAAGGTTGGATATCAAATCAAAATGAGAGAAGATGAAGACAGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

481

Amino Acids

55.25

Weight (kDa)

8.51

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 61 - 154 5.9e-16 Domain associated at C-terminal with AAA
AAA PF00004 261 - 396 8.4e-21 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 398 - 464 3.9e-22 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019425)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0020261
rosa_laevigata RLG00000032513
rosa_multiflora Rmu_sc0002087.1_g000008
rosa_roxburghii Rroxscaffold_1G00057950
rosa_rugosa Rorug05G0058000
rosa_samantha Rh5AG148500 Rh5CG159400
rosa_wichuraiana Rw5G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 310
AccII CGCG 1 cut(s) 1222
AciI CCGC 1 cut(s) 960
AclWI GGATC 4 cut(s) 112, 680, 693, 1244
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 716, 986
AfaI GTAC 2 cut(s) 180, 909
AfiI CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
AflIII ACRYGT 2 cut(s) 426, 1182
AgeI ACCGGT 1 cut(s) 723
AgsI TTSAA 7 cut(s) 169, 422, 471, 550, 853, 883, 1271
AhlI ACTAGT 3 cut(s) 262, 383, 910
AjiI CACGTC 1 cut(s) 427
AjnI CCWGG 1 cut(s) 371
AloI GAACNNNNNNTCC 2 cut(s) 445, 477
AluBI AGCT 8 cut(s) 53, 445, 483, 490, 694, 827, 871, 970
AluI AGCT 8 cut(s) 53, 445, 483, 490, 694, 827, 871, 970
Alw21I GWGCWC 2 cut(s) 318, 599
Alw26I GTCTC 1 cut(s) 372
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 4 cut(s) 112, 680, 693, 1244
AoxI GGCC 3 cut(s) 561, 1051, 1072
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 5 cut(s) 50, 827, 868, 1150, 1279
ApoI RAATTY 1 cut(s) 297
AsiGI ACCGGT 1 cut(s) 723
AspLEI GCGC 3 cut(s) 1222, 1224, 1315
AspS9I GGNCC 2 cut(s) 721, 794
AsuC2I CCSGG 2 cut(s) 801, 1158
AsuII TTCGAA 2 cut(s) 1007, 1209
AsuNHI GCTAGC 1 cut(s) 46
AvaII GGWCC 2 cut(s) 721, 794
BaeGI GKGCMC 1 cut(s) 599
BamHI GGATCC 1 cut(s) 685
BbsI GAAGAC 3 cut(s) 429, 704, 1437
Bbv12I GWGCWC 2 cut(s) 318, 599
BbvCI CCTCAGC 1 cut(s) 1373
BbvI GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
BccI CCATC 2 cut(s) 257, 1064
BciT130I CCWGG 1 cut(s) 373
BclI TGATCA 1 cut(s) 1362
BcnI CCSGG 2 cut(s) 801, 1158
BcoDI GTCTC 1 cut(s) 372
BcuI ACTAGT 3 cut(s) 262, 383, 910
BfaI CTAG 6 cut(s) 47, 263, 384, 677, 911, 1441
BisI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
BlsI GCNGC 5 cut(s) 52, 829, 870, 1152, 1281
Bme1390I CCNGG 3 cut(s) 373, 801, 1158
Bme18I GGWCC 2 cut(s) 721, 794
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 721, 794
BmiI GGNNCC 2 cut(s) 687, 796
BmrFI CCNGG 3 cut(s) 373, 801, 1158
BmsI GCATC 4 cut(s) 13, 68, 69, 1327
BmtI GCTAGC 1 cut(s) 50
BpiI GAAGAC 3 cut(s) 429, 704, 1437
BpmI CTGGAG 2 cut(s) 116, 211
Bpu10I CCTNAGC 1 cut(s) 1373
Bpu14I TTCGAA 2 cut(s) 1007, 1209
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 2 cut(s) 801, 1158
Bsa29I ATCGAT 1 cut(s) 704
BsaJI CCNNGG 6 cut(s) 277, 681, 751, 799, 1215, 1354
BsaWI WCCGGW 1 cut(s) 723
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
Bse118I RCCGGY 2 cut(s) 723, 1033
Bse1I ACTGG 1 cut(s) 811
Bse3DI GCAATG 1 cut(s) 1145
BseBI CCWGG 1 cut(s) 373
BseCI ATCGAT 1 cut(s) 704
BseDI CCNNGG 6 cut(s) 277, 681, 751, 799, 1215, 1354
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
BseMI GCAATG 1 cut(s) 1145
BseMII CTCAG 2 cut(s) 913, 1364
BseNI ACTGG 1 cut(s) 811
BsePI GCGCGC 1 cut(s) 1220
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
BsgI GTGCAG 2 cut(s) 1287, 1332
Bsh1236I CGCG 1 cut(s) 1222
BshFI GGCC 3 cut(s) 563, 1053, 1074
BshTI ACCGGT 1 cut(s) 723
BshVI ATCGAT 1 cut(s) 704
BsiHKAI GWGCWC 2 cut(s) 318, 599
BsiSI CCGG 4 cut(s) 724, 801, 1034, 1158
BslFI GGGAC 3 cut(s) 8, 780, 1140
BslI CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 3 cut(s) 8, 780, 1140
BsnI GGCC 3 cut(s) 563, 1053, 1074
Bsp119I TTCGAA 2 cut(s) 1007, 1209
Bsp1286I GDGCHC 2 cut(s) 318, 599
Bsp143I GATC 5 cut(s) 117, 685, 1236, 1362, 1383
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 1 cut(s) 960
BspANI GGCC 3 cut(s) 563, 1053, 1074
BspCNI CTCAG 2 cut(s) 912, 1365
BspDI ATCGAT 1 cut(s) 704
BspFNI CGCG 1 cut(s) 1222
BspHI TCATGA 2 cut(s) 241, 252
BspLI GGNNCC 2 cut(s) 687, 796
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 4 cut(s) 112, 680, 693, 1244
BspT104I TTCGAA 2 cut(s) 1007, 1209
BsrDI GCAATG 1 cut(s) 1145
BsrFI RCCGGY 2 cut(s) 723, 1033
BsrI ACTGG 1 cut(s) 811
BssAI RCCGGY 2 cut(s) 723, 1033
BssECI CCNNGG 6 cut(s) 277, 681, 751, 799, 1215, 1354
BssHII GCGCGC 1 cut(s) 1220
BssMI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
BssT1I CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 1436
Bst6I CTCTTC 3 cut(s) 545, 767, 1322
BstAPI GCANNNNNTGC 2 cut(s) 56, 322
BstBI TTCGAA 2 cut(s) 1007, 1209
BstC8I GCNNGC 4 cut(s) 48, 1035, 1222, 1277
BstDEI CTNAG 2 cut(s) 899, 1373
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 1 cut(s) 1222
BstHHI GCGC 3 cut(s) 1222, 1224, 1315
BstKTI GATC 5 cut(s) 120, 688, 1239, 1365, 1386
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
BstMWI GCNNNNNNNGC 9 cut(s) 56, 322, 336, 373, 668, 677, 833, 1310, 1346
BstNI CCWGG 1 cut(s) 373
BstNSI RCATGY 1 cut(s) 1186
BstSCI CCNGG 3 cut(s) 371, 799, 1156
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 1 cut(s) 1222
BstV1I GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
BstV2I GAAGAC 3 cut(s) 429, 704, 1437
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
Bsu15I ATCGAT 1 cut(s) 704
BsuRI GGCC 3 cut(s) 563, 1053, 1074
BsuTUI ATCGAT 1 cut(s) 704
BtgI CCRYGG 1 cut(s) 277
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 3 cut(s) 525, 804, 1441
Cac8I GCNNGC 4 cut(s) 48, 1035, 1222, 1277
CciI TCATGA 2 cut(s) 241, 252
CfoI GCGC 3 cut(s) 1222, 1224, 1315
Cfr10I RCCGGY 2 cut(s) 723, 1033
Cfr13I GGNCC 2 cut(s) 721, 794
ClaI ATCGAT 1 cut(s) 704
CseI GACGC 1 cut(s) 1142
Csp6I GTAC 2 cut(s) 179, 908
CspAI ACCGGT 1 cut(s) 723
CviAII CATG 9 cut(s) 76, 94, 218, 242, 253, 278, 305, 565, 1183
CviQI GTAC 2 cut(s) 179, 908
DdeI CTNAG 2 cut(s) 899, 1373
DpnI GATC 5 cut(s) 119, 687, 1238, 1364, 1385
DpnII GATC 5 cut(s) 117, 685, 1236, 1362, 1383
Eam1104I CTCTTC 3 cut(s) 545, 767, 1322
EarI CTCTTC 3 cut(s) 545, 767, 1322
Eco130I CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
Eco32I GATATC 1 cut(s) 1406
Eco47I GGWCC 2 cut(s) 721, 794
Eco57I CTGAAG 2 cut(s) 716, 986
EcoRII CCWGG 1 cut(s) 371
EcoRV GATATC 1 cut(s) 1406
EcoT14I CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
EcoT22I ATGCAT 2 cut(s) 6, 1176
ErhI CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
FaeI CATG 9 cut(s) 79, 97, 221, 245, 256, 281, 308, 568, 1186
FaqI GGGAC 3 cut(s) 8, 780, 1140
FatI CATG 9 cut(s) 75, 93, 217, 241, 252, 277, 304, 564, 1182
FbaI TGATCA 1 cut(s) 1362
Fnu4HI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
FspBI CTAG 6 cut(s) 47, 263, 384, 677, 911, 1441
GlaI GCGC 3 cut(s) 1221, 1223, 1314
GluI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
GsuI CTGGAG 2 cut(s) 116, 211
HaeIII GGCC 3 cut(s) 563, 1053, 1074
HapII CCGG 4 cut(s) 724, 801, 1034, 1158
HgaI GACGC 1 cut(s) 1142
HhaI GCGC 3 cut(s) 1222, 1224, 1315
Hin1II CATG 9 cut(s) 79, 97, 221, 245, 256, 281, 308, 568, 1186
Hin6I GCGC 3 cut(s) 1220, 1222, 1313
HinP1I GCGC 3 cut(s) 1220, 1222, 1313
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 1 cut(s) 234
HpaII CCGG 4 cut(s) 724, 801, 1034, 1158
Hpy166II GTNNAC 3 cut(s) 179, 406, 597
Hpy188I TCNGA 6 cut(s) 15, 650, 889, 902, 966, 1362
Hpy188III TCNNGA 4 cut(s) 133, 228, 242, 253
Hpy8I GTNNAC 3 cut(s) 179, 406, 597
HpyAV CCTTC 3 cut(s) 223, 727, 756
HpyCH4III ACNGT 1 cut(s) 1436
HpyCH4IV ACGT 2 cut(s) 426, 501
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 322, 336, 373, 668, 677, 833, 1310, 1346
HpyF3I CTNAG 2 cut(s) 899, 1373
HpySE526I ACGT 2 cut(s) 426, 501
Hsp92II CATG 9 cut(s) 79, 97, 221, 245, 256, 281, 308, 568, 1186
HspAI GCGC 3 cut(s) 1220, 1222, 1313
KroI GCCGGC 1 cut(s) 1033
KroNI GCCGGC 1 cut(s) 1035
Ksp22I TGATCA 1 cut(s) 1362
Kzo9I GATC 5 cut(s) 117, 685, 1236, 1362, 1383
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
LweI GCATC 4 cut(s) 13, 68, 69, 1327
MaeI CTAG 6 cut(s) 47, 263, 384, 677, 911, 1441
MaeII ACGT 2 cut(s) 426, 501
MaeIII GTNAC 4 cut(s) 287, 1294, 1331, 1436
MalI GATC 5 cut(s) 119, 687, 1238, 1364, 1385
MboI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
MfeI CAATTG 1 cut(s) 831
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 318, 599
MluCI AATT 5 cut(s) 103, 297, 398, 831, 1225
MmeI TCCRAC 1 cut(s) 1381
Mph1103I ATGCAT 2 cut(s) 6, 1176
MroNI GCCGGC 1 cut(s) 1033
MseI TTAA 3 cut(s) 357, 558, 990
MslI CAYNNNNRTG 2 cut(s) 40, 938
MspI CCGG 4 cut(s) 724, 801, 1034, 1158
MspR9I CCNGG 3 cut(s) 373, 801, 1158
MunI CAATTG 1 cut(s) 831
MvaI CCWGG 1 cut(s) 373
MvnI CGCG 1 cut(s) 1222
MwoI GCNNNNNNNGC 9 cut(s) 56, 322, 336, 373, 668, 677, 833, 1310, 1346
NaeI GCCGGC 1 cut(s) 1035
NciI CCSGG 2 cut(s) 801, 1158
NcoI CCATGG 1 cut(s) 277
NdeII GATC 5 cut(s) 117, 685, 1236, 1362, 1383
NgoMIV GCCGGC 1 cut(s) 1033
NheI GCTAGC 1 cut(s) 46
NlaIII CATG 9 cut(s) 79, 97, 221, 245, 256, 281, 308, 568, 1186
NlaIV GGNNCC 2 cut(s) 687, 796
NmuCI GTSAC 4 cut(s) 287, 1294, 1331, 1436
NsiI ATGCAT 2 cut(s) 6, 1176
NspI RCATGY 1 cut(s) 1186
NspV TTCGAA 2 cut(s) 1007, 1209
PagI TCATGA 2 cut(s) 241, 252
PauI GCGCGC 1 cut(s) 1220
PciI ACATGT 1 cut(s) 1182
PdiI GCCGGC 1 cut(s) 1035
PfeI GAWTC 1 cut(s) 234
PflMI CCANNNNNTGG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 1156
PinAI ACCGGT 1 cut(s) 723
PkrI GCNGC 5 cut(s) 52, 829, 870, 1152, 1281
PscI ACATGT 1 cut(s) 1182
Psp6I CCWGG 1 cut(s) 371
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 687, 796
PspPI GGNCC 2 cut(s) 721, 794
PsuI RGATCY 1 cut(s) 685
PteI GCGCGC 1 cut(s) 1220
RsaI GTAC 2 cut(s) 180, 909
RsaNI GTAC 2 cut(s) 179, 908
RseI CAYNNNNRTG 2 cut(s) 40, 938
SaqAI TTAA 3 cut(s) 357, 558, 990
SatI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
Sau3AI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
Sau96I GGNCC 2 cut(s) 721, 794
ScrFI CCNGG 3 cut(s) 373, 801, 1158
SduI GDGCHC 2 cut(s) 318, 599
SfaNI GCATC 4 cut(s) 13, 68, 69, 1327
SfuI TTCGAA 2 cut(s) 1007, 1209
SinI GGWCC 2 cut(s) 721, 794
SmiMI CAYNNNNRTG 2 cut(s) 40, 938
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SpeI ACTAGT 3 cut(s) 262, 383, 910
Sse9I AATT 5 cut(s) 103, 297, 398, 831, 1225
SsiI CCGC 1 cut(s) 960
SspMI CTAG 6 cut(s) 47, 263, 384, 677, 911, 1441
StyD4I CCNGG 3 cut(s) 371, 799, 1156
StyI CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
TaaI ACNGT 1 cut(s) 1436
TaiI ACGT 2 cut(s) 429, 504
TaqI TCGA 5 cut(s) 349, 704, 1007, 1209, 1386
TasI AATT 5 cut(s) 103, 297, 398, 831, 1225
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 3 cut(s) 357, 558, 990
Tru9I TTAA 3 cut(s) 357, 558, 990
TscAI CASTG 3 cut(s) 525, 811, 1441
TseFI GTSAC 4 cut(s) 287, 1294, 1331, 1436
TseI GCWGC 5 cut(s) 50, 827, 868, 1150, 1279
Tsp45I GTSAC 4 cut(s) 287, 1294, 1331, 1436
TspDTI ATGAA 6 cut(s) 178, 230, 581, 1054, 1340, 1443
TspRI CASTG 3 cut(s) 525, 811, 1441
Van91I CCANNNNNTGG 1 cut(s) 310
VneI GTGCAC 1 cut(s) 595
VpaK11BI GGWCC 2 cut(s) 721, 794
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 1186
XspI CTAG 6 cut(s) 47, 263, 384, 677, 911, 1441
Zsp2I ATGCAT 2 cut(s) 6, 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.