Rh5AG148500

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019425
Physical Location & Seq
Reverse (-)
15606716 .. 15609061
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG148500.1

Sequence Viewer

Length: 1389 bp
ATGCATCTTCCTCTGAAAAAAGTCCCCATTTACACACCAAAATGGCTAGCAGCTTGTGCATCAATGTTTGCTTCCTTGATGCTTATTCAGTCCATGACTAACAATTTTTATACCTTGATCCCCCAACCCCTCCAGAGGTTTGTCATTTCCATTGTCATCGGCTTCTTCAAACGAAGTGTACCCCTTTTCATCCAAAGGCTTCTCCTTTCAGCCTTCCATGAGTTCTCCAGACGATTCTTTCATGACCCCATCATGACCTTACTAGTTGAGGAATACCATGGCTTTTGTGACAACGAAATTTACCATGCTGTGGAGCACTACTTGCATAGCAAAATCAGCCCAAATACTCGACTCCTTAAAATAGCGCAAACCTGGCGAGACACTAGTTCCTCCTACCAATTTGTTGACAGGCAAGAGTTTGAAGACTTGTATGAGGGCATTGAGCTGAAATGGAAGTTCAGTTCCACTTCAAATCAGCAAAGCTTCCAGCTTATGTTTGACGCAAGGCACAAGGAAGCAGTGGGGCGTTCTTACATTCCTTATGTTTTGAAGAGGTTTCAGGCCATGAAAGAGGAAACAAAGATTGTCAGGCTGTGCACACTTGCGAGGAAAAATGGGCATAACATCAAGTGGCAATCAATAACATTCGGAAACTACTCTGCCACATTTGCTACTCTAGCCTTGGATCCCAAGCTGAAGACATCGATTTTAGAAGATTTGGACCGGTTTGTGAAGGGCAAACAGTTCTACCAAGGTGTAGGAAGGGCTTGGAAAAGAGGCTATCTTCTATATGGTCCCCCCGGCACCGGTAAATCCTCCCTGATAGCTGCAATTGCTAATCATCTCAAGTTCAACATCTATGACTTGCAGCTCTCACATTTGAAATCCGATGTGGAACTGAGAAGTGTACTAGTTGCAACCACAAACCAATCCATACTTGTGATTGAGGATATAGATTGCGGTCTGAAGCTACAAGATAGGACCCTTGTTAAGACAACCAGCACTTCGAAAGAGAAAGACCGAGCAGATGAGCCGGCGATTACACTTTCTGGCCTACTGAACTTCATAGATGGCCTATGGTCTGGTTCAGCAGAGCAAAGGATAATCATATTCACTACCAATCACAAGGACAAGCTTGACCCTGCATTGCTGCGTCCCGGACGAATGGATATGCAAATCCACATGTCATATTGCACCTTTGGTGGGTTCGAAACCTTGGCACGCAATTACTTGGGGATCAAAGAACACCAGCCTTTGTTTGATGAGATTGAAAACCTGCTGCAAAATGCAGAGGTCACTCCTGCACAAGTGGCGCAAGAGCTGATGAAGAGTGAGGATGCTGAAGTTGCACTCCAAGGTCTGATCACAATGCTGAGGGAAAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

462

Amino Acids

53.1

Weight (kDa)

8.83

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 61 - 154 1.1e-15 Domain associated at C-terminal with AAA
AAA PF00004 261 - 396 1.2e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 398 - 461 3.3e-21 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019425)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0020261
rosa_laevigata RLG00000032513
rosa_multiflora Rmu_sc0002087.1_g000008
rosa_roxburghii Rroxscaffold_1G00057950
rosa_rugosa Rorug05G0058000
rosa_samantha Rh5AG148500 Rh5CG159400
rosa_wichuraiana Rw5G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1284
AccB1I GGYRCC 1 cut(s) 803
AccB7I CCANNNNNTGG 1 cut(s) 310
AciI CCGC 1 cut(s) 960
AclWI GGATC 4 cut(s) 112, 680, 693, 1244
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 3 cut(s) 716, 986, 1362
AfaI GTAC 2 cut(s) 180, 909
AfiI CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
AflIII ACRYGT 1 cut(s) 1182
AgeI ACCGGT 2 cut(s) 723, 806
AgsI TTSAA 7 cut(s) 169, 422, 471, 550, 853, 883, 1271
AhlI ACTAGT 3 cut(s) 262, 383, 910
AjnI CCWGG 1 cut(s) 371
AloI GAACNNNNNNTCC 2 cut(s) 445, 477
Alw21I GWGCWC 2 cut(s) 318, 599
Alw26I GTCTC 1 cut(s) 372
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 4 cut(s) 112, 680, 693, 1244
AoxI GGCC 3 cut(s) 561, 1051, 1072
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 5 cut(s) 50, 827, 868, 1150, 1279
ApoI RAATTY 1 cut(s) 297
AsiGI ACCGGT 2 cut(s) 723, 806
AspLEI GCGC 2 cut(s) 367, 1315
AspS9I GGNCC 3 cut(s) 721, 794, 981
AsuC2I CCSGG 2 cut(s) 801, 1158
AsuII TTCGAA 2 cut(s) 1007, 1209
AsuNHI GCTAGC 1 cut(s) 46
AvaII GGWCC 3 cut(s) 721, 794, 981
BaeGI GKGCMC 1 cut(s) 599
BamHI GGATCC 1 cut(s) 685
BanI GGYRCC 1 cut(s) 803
BbsI GAAGAC 2 cut(s) 429, 704
Bbv12I GWGCWC 2 cut(s) 318, 599
BbvCI CCTCAGC 1 cut(s) 1373
BbvI GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
BccI CCATC 2 cut(s) 257, 1064
BciT130I CCWGG 1 cut(s) 373
BclI TGATCA 1 cut(s) 1362
BcnI CCSGG 2 cut(s) 801, 1158
BcoDI GTCTC 1 cut(s) 372
BcuI ACTAGT 3 cut(s) 262, 383, 910
BfaI CTAG 5 cut(s) 47, 263, 384, 677, 911
BfuAI ACCTGC 1 cut(s) 1284
BglII AGATCT 1 cut(s) 1383
BisI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
BlsI GCNGC 5 cut(s) 52, 829, 870, 1152, 1281
Bme1390I CCNGG 3 cut(s) 373, 801, 1158
Bme18I GGWCC 3 cut(s) 721, 794, 981
BmgT120I GGNCC 3 cut(s) 721, 794, 981
BmiI GGNNCC 4 cut(s) 687, 796, 805, 983
BmrFI CCNGG 3 cut(s) 373, 801, 1158
BmsI GCATC 4 cut(s) 13, 68, 69, 1327
BmtI GCTAGC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 429, 704
BpmI CTGGAG 2 cut(s) 116, 211
Bpu10I CCTNAGC 1 cut(s) 1373
Bpu14I TTCGAA 2 cut(s) 1007, 1209
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 2 cut(s) 801, 1158
Bsa29I ATCGAT 1 cut(s) 704
BsaJI CCNNGG 6 cut(s) 277, 681, 751, 799, 1215, 1354
BsaWI WCCGGW 2 cut(s) 723, 806
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
Bse118I RCCGGY 3 cut(s) 723, 806, 1033
Bse3DI GCAATG 1 cut(s) 1145
BseBI CCWGG 1 cut(s) 373
BseCI ATCGAT 1 cut(s) 704
BseDI CCNNGG 6 cut(s) 277, 681, 751, 799, 1215, 1354
BseGI GGATG 2 cut(s) 189, 1342
BseLI CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
BseMI GCAATG 1 cut(s) 1145
BseMII CTCAG 2 cut(s) 890, 1364
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
BsgI GTGCAG 1 cut(s) 1287
BshFI GGCC 3 cut(s) 563, 1053, 1074
BshNI GGYRCC 1 cut(s) 803
BshTI ACCGGT 2 cut(s) 723, 806
BshVI ATCGAT 1 cut(s) 704
BsiHKAI GWGCWC 2 cut(s) 318, 599
BsiSI CCGG 5 cut(s) 724, 801, 807, 1034, 1158
BslFI GGGAC 3 cut(s) 8, 780, 1140
BslI CCNNNNNNNGG 4 cut(s) 135, 310, 806, 1203
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 3 cut(s) 8, 780, 1140
BsnI GGCC 3 cut(s) 563, 1053, 1074
Bsp119I TTCGAA 2 cut(s) 1007, 1209
Bsp1286I GDGCHC 2 cut(s) 318, 599
Bsp143I GATC 5 cut(s) 117, 685, 1236, 1362, 1383
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 1 cut(s) 960
BspANI GGCC 3 cut(s) 563, 1053, 1074
BspCNI CTCAG 2 cut(s) 891, 1365
BspDI ATCGAT 1 cut(s) 704
BspHI TCATGA 2 cut(s) 241, 252
BspLI GGNNCC 4 cut(s) 687, 796, 805, 983
BspMI ACCTGC 1 cut(s) 1284
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 4 cut(s) 112, 680, 693, 1244
BspT104I TTCGAA 2 cut(s) 1007, 1209
BspT107I GGYRCC 1 cut(s) 803
BsrDI GCAATG 1 cut(s) 1145
BsrFI RCCGGY 3 cut(s) 723, 806, 1033
BssAI RCCGGY 3 cut(s) 723, 806, 1033
BssECI CCNNGG 6 cut(s) 277, 681, 751, 799, 1215, 1354
BssMI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
BssT1I CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 744
Bst6I CTCTTC 2 cut(s) 545, 1322
BstAPI GCANNNNNTGC 2 cut(s) 56, 322
BstBI TTCGAA 2 cut(s) 1007, 1209
BstC8I GCNNGC 3 cut(s) 48, 1035, 1222
BstDEI CTNAG 2 cut(s) 899, 1373
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 189, 1342
BstHHI GCGC 2 cut(s) 367, 1315
BstKTI GATC 5 cut(s) 120, 688, 1239, 1365, 1386
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
BstMWI GCNNNNNNNGC 9 cut(s) 56, 322, 336, 373, 668, 677, 833, 1310, 1346
BstNI CCWGG 1 cut(s) 373
BstNSI RCATGY 1 cut(s) 1186
BstSCI CCNGG 3 cut(s) 371, 799, 1156
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
BstV2I GAAGAC 2 cut(s) 429, 704
BstX2I RGATCY 2 cut(s) 685, 1383
BstYI RGATCY 2 cut(s) 685, 1383
Bsu15I ATCGAT 1 cut(s) 704
BsuRI GGCC 3 cut(s) 563, 1053, 1074
BsuTUI ATCGAT 1 cut(s) 704
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 189, 1342
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 525
BveI ACCTGC 1 cut(s) 1284
Cac8I GCNNGC 3 cut(s) 48, 1035, 1222
CciI TCATGA 2 cut(s) 241, 252
CfoI GCGC 2 cut(s) 367, 1315
Cfr10I RCCGGY 3 cut(s) 723, 806, 1033
Cfr13I GGNCC 3 cut(s) 721, 794, 981
ClaI ATCGAT 1 cut(s) 704
CseI GACGC 2 cut(s) 509, 1142
Csp6I GTAC 2 cut(s) 179, 908
CspAI ACCGGT 2 cut(s) 723, 806
CviAII CATG 8 cut(s) 94, 218, 242, 253, 278, 305, 565, 1183
CviQI GTAC 2 cut(s) 179, 908
DdeI CTNAG 2 cut(s) 899, 1373
DpnI GATC 5 cut(s) 119, 687, 1238, 1364, 1385
DpnII GATC 5 cut(s) 117, 685, 1236, 1362, 1383
Eam1104I CTCTTC 2 cut(s) 545, 1322
EarI CTCTTC 2 cut(s) 545, 1322
Eco130I CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
Eco47I GGWCC 3 cut(s) 721, 794, 981
Eco57I CTGAAG 3 cut(s) 716, 986, 1362
EcoO109I RGGNCCY 1 cut(s) 981
EcoRII CCWGG 1 cut(s) 371
EcoT14I CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
FaeI CATG 8 cut(s) 97, 221, 245, 256, 281, 308, 568, 1186
FaqI GGGAC 3 cut(s) 8, 780, 1140
FatI CATG 8 cut(s) 93, 217, 241, 252, 277, 304, 564, 1182
FbaI TGATCA 1 cut(s) 1362
Fnu4HI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
FokI GGATG 2 cut(s) 176, 1349
Fsp4HI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
FspBI CTAG 5 cut(s) 47, 263, 384, 677, 911
GlaI GCGC 2 cut(s) 366, 1314
GluI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
GsuI CTGGAG 2 cut(s) 116, 211
HaeIII GGCC 3 cut(s) 563, 1053, 1074
HapII CCGG 5 cut(s) 724, 801, 807, 1034, 1158
HgaI GACGC 2 cut(s) 509, 1142
HhaI GCGC 2 cut(s) 367, 1315
Hin1II CATG 8 cut(s) 97, 221, 245, 256, 281, 308, 568, 1186
Hin6I GCGC 2 cut(s) 365, 1313
HinP1I GCGC 2 cut(s) 365, 1313
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 2 cut(s) 481, 1133
HinfI GANTC 2 cut(s) 234, 351
HpaII CCGG 5 cut(s) 724, 801, 807, 1034, 1158
Hpy166II GTNNAC 4 cut(s) 179, 406, 597, 908
Hpy188I TCNGA 5 cut(s) 15, 650, 889, 966, 1362
Hpy188III TCNNGA 4 cut(s) 133, 228, 242, 253
Hpy8I GTNNAC 4 cut(s) 179, 406, 597, 908
HpyAV CCTTC 3 cut(s) 223, 727, 756
HpyCH4III ACNGT 1 cut(s) 744
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 322, 336, 373, 668, 677, 833, 1310, 1346
HpyF3I CTNAG 2 cut(s) 899, 1373
Hsp92II CATG 8 cut(s) 97, 221, 245, 256, 281, 308, 568, 1186
HspAI GCGC 2 cut(s) 365, 1313
KroI GCCGGC 1 cut(s) 1033
KroNI GCCGGC 1 cut(s) 1035
Ksp22I TGATCA 1 cut(s) 1362
Kzo9I GATC 5 cut(s) 117, 685, 1236, 1362, 1383
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 5 cut(s) 62, 814, 880, 1137, 1266
LweI GCATC 4 cut(s) 13, 68, 69, 1327
MaeI CTAG 5 cut(s) 47, 263, 384, 677, 911
MaeIII GTNAC 2 cut(s) 287, 1294
MalI GATC 5 cut(s) 119, 687, 1238, 1364, 1385
MboI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
MboII GAAGA 7 cut(s) 157, 434, 562, 709, 725, 776, 1339
MfeI CAATTG 1 cut(s) 831
MflI RGATCY 2 cut(s) 685, 1383
MhlI GDGCHC 2 cut(s) 318, 599
MluCI AATT 5 cut(s) 103, 297, 398, 831, 1225
MlyI GAGTC 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 1033
MseI TTAA 2 cut(s) 357, 990
MslI CAYNNNNRTG 2 cut(s) 40, 938
MspI CCGG 5 cut(s) 724, 801, 807, 1034, 1158
MspR9I CCNGG 3 cut(s) 373, 801, 1158
MunI CAATTG 1 cut(s) 831
MvaI CCWGG 1 cut(s) 373
MwoI GCNNNNNNNGC 9 cut(s) 56, 322, 336, 373, 668, 677, 833, 1310, 1346
NaeI GCCGGC 1 cut(s) 1035
NciI CCSGG 2 cut(s) 801, 1158
NcoI CCATGG 1 cut(s) 277
NdeII GATC 5 cut(s) 117, 685, 1236, 1362, 1383
NgoMIV GCCGGC 1 cut(s) 1033
NheI GCTAGC 1 cut(s) 46
NlaIII CATG 8 cut(s) 97, 221, 245, 256, 281, 308, 568, 1186
NlaIV GGNNCC 4 cut(s) 687, 796, 805, 983
NmuCI GTSAC 2 cut(s) 287, 1294
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1186
NspV TTCGAA 2 cut(s) 1007, 1209
PagI TCATGA 2 cut(s) 241, 252
PciI ACATGT 1 cut(s) 1182
PdiI GCCGGC 1 cut(s) 1035
PfeI GAWTC 1 cut(s) 234
PflMI CCANNNNNTGG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 1156
PinAI ACCGGT 2 cut(s) 723, 806
PkrI GCNGC 5 cut(s) 52, 829, 870, 1152, 1281
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
PpuMI RGGWCCY 1 cut(s) 981
PscI ACATGT 1 cut(s) 1182
Psp5II RGGWCCY 1 cut(s) 981
Psp6I CCWGG 1 cut(s) 371
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 4 cut(s) 687, 796, 805, 983
PspPI GGNCC 3 cut(s) 721, 794, 981
PspPPI RGGWCCY 1 cut(s) 981
PsuI RGATCY 2 cut(s) 685, 1383
RsaI GTAC 2 cut(s) 180, 909
RsaNI GTAC 2 cut(s) 179, 908
RseI CAYNNNNRTG 2 cut(s) 40, 938
SaqAI TTAA 2 cut(s) 357, 990
SatI GCNGC 5 cut(s) 51, 828, 869, 1151, 1280
Sau3AI GATC 5 cut(s) 117, 685, 1236, 1362, 1383
Sau96I GGNCC 3 cut(s) 721, 794, 981
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 3 cut(s) 373, 801, 1158
SduI GDGCHC 2 cut(s) 318, 599
SfaNI GCATC 4 cut(s) 13, 68, 69, 1327
SfuI TTCGAA 2 cut(s) 1007, 1209
SinI GGWCC 3 cut(s) 721, 794, 981
SmiMI CAYNNNNRTG 2 cut(s) 40, 938
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SpeI ACTAGT 3 cut(s) 262, 383, 910
Sse9I AATT 5 cut(s) 103, 297, 398, 831, 1225
SsiI CCGC 1 cut(s) 960
SspMI CTAG 5 cut(s) 47, 263, 384, 677, 911
StyD4I CCNGG 3 cut(s) 371, 799, 1156
StyI CCWWGG 5 cut(s) 277, 681, 751, 1215, 1354
TaaI ACNGT 1 cut(s) 744
TaqI TCGA 4 cut(s) 349, 704, 1007, 1209
TaqII GACCGA 1 cut(s) 1035
TasI AATT 5 cut(s) 103, 297, 398, 831, 1225
TatI WGTACW 1 cut(s) 907
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 2 cut(s) 357, 990
Tru9I TTAA 2 cut(s) 357, 990
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 2 cut(s) 287, 1294
TseI GCWGC 5 cut(s) 50, 827, 868, 1150, 1279
Tsp45I GTSAC 2 cut(s) 287, 1294
TspDTI ATGAA 5 cut(s) 178, 230, 581, 1054, 1340
TspRI CASTG 1 cut(s) 525
Van91I CCANNNNNTGG 1 cut(s) 310
VneI GTGCAC 1 cut(s) 595
VpaK11BI GGWCC 3 cut(s) 721, 794, 981
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 1186
XspI CTAG 5 cut(s) 47, 263, 384, 677, 911
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.