Rw5G013140

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0019425
Physical Location & Seq
Reverse (-)
15484974 .. 15486572
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013140.1

Sequence Viewer

Length: 1326 bp
ATGTTTGCTTCCTTGATGCTTATTCAGTCCATGACTAACAATTTTTATACCTTGATCCCCCAACCCCTCCAGAGGTTTGTCATTTCCATTGTCATCGGCTTCTTCAAACGAAGTGTACCCCTTTTCATCCAAAGGCTTCTCCTTTCAGCCTTCCATGAGTTCTCCAGACGATTCTTTCATGACCCCATCATGACCTTACTAGTTGAGGAATACCATGGCTTTTGTGACAACGAAATTTACCATGCTGTGGAGCACTACTTGCATAGCAAAATCAGCCCAAATACTCGACTCCTTAAAATAGCGCAAACCTGGCGAGACACTAGTTCCTCCTACCAATTTGTTGACAGGCAAGAGTTTGAAGACTTGTATGAGGGCATTGAGCTGAAATGGAAGTTCAGTTCCACTTCAAATCAGCAAAGCTTCCAGCTTATGTTTGACGCAAGGCACAAGGAAGCAGTGGGGCGTTCTTACATTCCTTATGTTTTGAAGAGGTTTCAGGCCATGAAAGAGGAAACAAAGATTGTCAGGCTGTGCACACTTGCGAGGAAAAATGGGCATAACATCAAGTGGCAATCAATAACATTCGGAAACTACTCTGCCACATTTGCTACTCTAGCCTTGGATCCCAAGCTGAAGACATCGATTTTAGAAGATTTGGACCGGTTTGTGAAGGGCAAACAGTTCTACCAAGGTGTAGGAAGGGCTTGGAAAAGAGGCTATCTTCTATATGGTCCCCCCGGCACCGGTAAATCCTCCCTGATAGCTGCAATTGCTAATCATCTCAAGTTCAACATCTATGACTTGCAGCTCTCACATTTGAAATCCGATGTGGAACTGAGAAGTGTACTAGTTGCAACCACAAACCAATCCATACTTGTGATTGAGGATATAGATTGCGGTCTGAAGCTACAAGATAGGACCCTTGTTAAGACAACCAGCACTTCGAAAGAGAAAGACCGAGCAGATGAGCCGGCGATTACACTTTCTGGCCTACTGAACTTCATAGATGGCCTATGGTCTGGTTCAGCAGAGCAAAGGATAATCATATTCACTACCAATCACAAGGACAAGCTTGACCCTGCATTGCTGCGTCCCGGACGAATGGATATGCAAATCCACATGTCATATTGCACCTTTGGTGGGTTCGAAACCTTGGCACGCAATTACTTGGGGATCAAAGAACACCAGCCTTTGTTTGATGAGATTGAAAACCTGCTGCAAAATGCAGAGGTCACTCCTGCACAAGTGGCGCAAGAGCTGATGAAGAGTGAGGATGCTGAAGTTGCACTCCAAGGTCTGATCACAATGCTGAGGGAAAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

441

Amino Acids

50.75

Weight (kDa)

8.45

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 40 - 133 1e-15 Domain associated at C-terminal with AAA
AAA PF00004 240 - 375 1.1e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 377 - 440 3.1e-21 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019425)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0020261
rosa_laevigata RLG00000032513
rosa_multiflora Rmu_sc0002087.1_g000008
rosa_roxburghii Rroxscaffold_1G00057950
rosa_rugosa Rorug05G0058000
rosa_samantha Rh5AG148500 Rh5CG159400
rosa_wichuraiana Rw5G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1221
AccB1I GGYRCC 1 cut(s) 740
AccB7I CCANNNNNTGG 1 cut(s) 247
AciI CCGC 1 cut(s) 897
AclWI GGATC 4 cut(s) 49, 617, 630, 1181
AcsI RAATTY 1 cut(s) 234
AcuI CTGAAG 3 cut(s) 653, 923, 1299
AfaI GTAC 2 cut(s) 117, 846
AfiI CCNNNNNNNGG 4 cut(s) 72, 247, 743, 1140
AflIII ACRYGT 1 cut(s) 1119
AgeI ACCGGT 2 cut(s) 660, 743
AgsI TTSAA 7 cut(s) 106, 359, 408, 487, 790, 820, 1208
AhlI ACTAGT 3 cut(s) 199, 320, 847
AjnI CCWGG 1 cut(s) 308
AloI GAACNNNNNNTCC 2 cut(s) 382, 414
AluBI AGCT 9 cut(s) 382, 420, 427, 631, 764, 808, 907, 1072, 1258
AluI AGCT 9 cut(s) 382, 420, 427, 631, 764, 808, 907, 1072, 1258
Alw21I GWGCWC 2 cut(s) 255, 536
Alw26I GTCTC 1 cut(s) 309
Alw44I GTGCAC 1 cut(s) 532
AlwI GGATC 4 cut(s) 49, 617, 630, 1181
AoxI GGCC 3 cut(s) 498, 988, 1009
ApaLI GTGCAC 1 cut(s) 532
ApeKI GCWGC 4 cut(s) 764, 805, 1087, 1216
ApoI RAATTY 1 cut(s) 234
AsiGI ACCGGT 2 cut(s) 660, 743
AspLEI GCGC 2 cut(s) 304, 1252
AspS9I GGNCC 3 cut(s) 658, 731, 918
AsuC2I CCSGG 2 cut(s) 738, 1095
AsuII TTCGAA 2 cut(s) 944, 1146
AvaII GGWCC 3 cut(s) 658, 731, 918
BaeGI GKGCMC 1 cut(s) 536
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 366, 641
Bbv12I GWGCWC 2 cut(s) 255, 536
BbvCI CCTCAGC 1 cut(s) 1310
BbvI GCAGC 4 cut(s) 751, 817, 1074, 1203
BccI CCATC 2 cut(s) 194, 1001
BciT130I CCWGG 1 cut(s) 310
BclI TGATCA 1 cut(s) 1299
BcnI CCSGG 2 cut(s) 738, 1095
BcoDI GTCTC 1 cut(s) 309
BcuI ACTAGT 3 cut(s) 199, 320, 847
BfaI CTAG 4 cut(s) 200, 321, 614, 848
BfuAI ACCTGC 1 cut(s) 1221
BglII AGATCT 1 cut(s) 1320
BisI GCNGC 4 cut(s) 765, 806, 1088, 1217
BlsI GCNGC 4 cut(s) 766, 807, 1089, 1218
Bme1390I CCNGG 3 cut(s) 310, 738, 1095
Bme18I GGWCC 3 cut(s) 658, 731, 918
BmgT120I GGNCC 3 cut(s) 658, 731, 918
BmiI GGNNCC 4 cut(s) 624, 733, 742, 920
BmrFI CCNGG 3 cut(s) 310, 738, 1095
BmsI GCATC 2 cut(s) 6, 1264
BpiI GAAGAC 2 cut(s) 366, 641
BpmI CTGGAG 2 cut(s) 53, 148
Bpu10I CCTNAGC 1 cut(s) 1310
Bpu14I TTCGAA 2 cut(s) 944, 1146
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 2 cut(s) 738, 1095
Bsa29I ATCGAT 1 cut(s) 641
BsaJI CCNNGG 6 cut(s) 214, 618, 688, 736, 1152, 1291
BsaWI WCCGGW 2 cut(s) 660, 743
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 247, 743, 1140
Bse118I RCCGGY 3 cut(s) 660, 743, 970
Bse3DI GCAATG 1 cut(s) 1082
BseBI CCWGG 1 cut(s) 310
BseCI ATCGAT 1 cut(s) 641
BseDI CCNNGG 6 cut(s) 214, 618, 688, 736, 1152, 1291
BseGI GGATG 2 cut(s) 126, 1279
BseLI CCNNNNNNNGG 4 cut(s) 72, 247, 743, 1140
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 2 cut(s) 827, 1301
BseSI GKGCMC 1 cut(s) 536
BseXI GCAGC 4 cut(s) 751, 817, 1074, 1203
BsgI GTGCAG 1 cut(s) 1224
BshFI GGCC 3 cut(s) 500, 990, 1011
BshNI GGYRCC 1 cut(s) 740
BshTI ACCGGT 2 cut(s) 660, 743
BshVI ATCGAT 1 cut(s) 641
BsiHKAI GWGCWC 2 cut(s) 255, 536
BsiSI CCGG 5 cut(s) 661, 738, 744, 971, 1095
BslFI GGGAC 2 cut(s) 717, 1077
BslI CCNNNNNNNGG 4 cut(s) 72, 247, 743, 1140
BsmAI GTCTC 1 cut(s) 309
BsmFI GGGAC 2 cut(s) 717, 1077
BsnI GGCC 3 cut(s) 500, 990, 1011
Bsp119I TTCGAA 2 cut(s) 944, 1146
Bsp1286I GDGCHC 2 cut(s) 255, 536
Bsp143I GATC 5 cut(s) 54, 622, 1173, 1299, 1320
Bsp19I CCATGG 1 cut(s) 214
BspACI CCGC 1 cut(s) 897
BspANI GGCC 3 cut(s) 500, 990, 1011
BspCNI CTCAG 2 cut(s) 828, 1302
BspDI ATCGAT 1 cut(s) 641
BspHI TCATGA 2 cut(s) 178, 189
BspLI GGNNCC 4 cut(s) 624, 733, 742, 920
BspMI ACCTGC 1 cut(s) 1221
BspPI GGATC 4 cut(s) 49, 617, 630, 1181
BspT104I TTCGAA 2 cut(s) 944, 1146
BspT107I GGYRCC 1 cut(s) 740
BsrDI GCAATG 1 cut(s) 1082
BsrFI RCCGGY 3 cut(s) 660, 743, 970
BssAI RCCGGY 3 cut(s) 660, 743, 970
BssECI CCNNGG 6 cut(s) 214, 618, 688, 736, 1152, 1291
BssMI GATC 5 cut(s) 54, 622, 1173, 1299, 1320
BssT1I CCWWGG 5 cut(s) 214, 618, 688, 1152, 1291
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 681
Bst6I CTCTTC 2 cut(s) 482, 1259
BstAPI GCANNNNNTGC 1 cut(s) 259
BstBI TTCGAA 2 cut(s) 944, 1146
BstC8I GCNNGC 2 cut(s) 972, 1159
BstDEI CTNAG 2 cut(s) 836, 1310
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 2 cut(s) 126, 1279
BstHHI GCGC 2 cut(s) 304, 1252
BstKTI GATC 5 cut(s) 57, 625, 1176, 1302, 1323
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 5 cut(s) 54, 622, 1173, 1299, 1320
BstMWI GCNNNNNNNGC 8 cut(s) 259, 273, 310, 605, 614, 770, 1247, 1283
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 1123
BstSCI CCNGG 3 cut(s) 308, 736, 1093
BstSLI GKGCMC 1 cut(s) 536
BstV1I GCAGC 4 cut(s) 751, 817, 1074, 1203
BstV2I GAAGAC 2 cut(s) 366, 641
BstX2I RGATCY 2 cut(s) 622, 1320
BstYI RGATCY 2 cut(s) 622, 1320
Bsu15I ATCGAT 1 cut(s) 641
BsuRI GGCC 3 cut(s) 500, 990, 1011
BsuTUI ATCGAT 1 cut(s) 641
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 2 cut(s) 126, 1279
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 462
BveI ACCTGC 1 cut(s) 1221
Cac8I GCNNGC 2 cut(s) 972, 1159
CciI TCATGA 2 cut(s) 178, 189
CfoI GCGC 2 cut(s) 304, 1252
Cfr10I RCCGGY 3 cut(s) 660, 743, 970
Cfr13I GGNCC 3 cut(s) 658, 731, 918
ClaI ATCGAT 1 cut(s) 641
CseI GACGC 2 cut(s) 446, 1079
Csp6I GTAC 2 cut(s) 116, 845
CspAI ACCGGT 2 cut(s) 660, 743
CviAII CATG 8 cut(s) 31, 155, 179, 190, 215, 242, 502, 1120
CviQI GTAC 2 cut(s) 116, 845
DdeI CTNAG 2 cut(s) 836, 1310
DpnI GATC 5 cut(s) 56, 624, 1175, 1301, 1322
DpnII GATC 5 cut(s) 54, 622, 1173, 1299, 1320
Eam1104I CTCTTC 2 cut(s) 482, 1259
EarI CTCTTC 2 cut(s) 482, 1259
Eco130I CCWWGG 5 cut(s) 214, 618, 688, 1152, 1291
Eco47I GGWCC 3 cut(s) 658, 731, 918
Eco57I CTGAAG 3 cut(s) 653, 923, 1299
EcoO109I RGGNCCY 1 cut(s) 918
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 5 cut(s) 214, 618, 688, 1152, 1291
ErhI CCWWGG 5 cut(s) 214, 618, 688, 1152, 1291
FaeI CATG 8 cut(s) 34, 158, 182, 193, 218, 245, 505, 1123
FaqI GGGAC 2 cut(s) 717, 1077
FatI CATG 8 cut(s) 30, 154, 178, 189, 214, 241, 501, 1119
FbaI TGATCA 1 cut(s) 1299
Fnu4HI GCNGC 4 cut(s) 765, 806, 1088, 1217
FokI GGATG 2 cut(s) 113, 1286
Fsp4HI GCNGC 4 cut(s) 765, 806, 1088, 1217
FspBI CTAG 4 cut(s) 200, 321, 614, 848
GlaI GCGC 2 cut(s) 303, 1251
GluI GCNGC 4 cut(s) 765, 806, 1088, 1217
GsuI CTGGAG 2 cut(s) 53, 148
HaeIII GGCC 3 cut(s) 500, 990, 1011
HapII CCGG 5 cut(s) 661, 738, 744, 971, 1095
HgaI GACGC 2 cut(s) 446, 1079
HhaI GCGC 2 cut(s) 304, 1252
Hin1II CATG 8 cut(s) 34, 158, 182, 193, 218, 245, 505, 1123
Hin6I GCGC 2 cut(s) 302, 1250
HinP1I GCGC 2 cut(s) 302, 1250
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 2 cut(s) 418, 1070
HinfI GANTC 2 cut(s) 171, 288
HpaII CCGG 5 cut(s) 661, 738, 744, 971, 1095
Hpy166II GTNNAC 4 cut(s) 116, 343, 534, 845
Hpy188I TCNGA 4 cut(s) 587, 826, 903, 1299
Hpy188III TCNNGA 4 cut(s) 70, 165, 179, 190
Hpy8I GTNNAC 4 cut(s) 116, 343, 534, 845
HpyAV CCTTC 3 cut(s) 160, 664, 693
HpyCH4III ACNGT 1 cut(s) 681
HpyF10VI GCNNNNNNNGC 8 cut(s) 259, 273, 310, 605, 614, 770, 1247, 1283
HpyF3I CTNAG 2 cut(s) 836, 1310
Hsp92II CATG 8 cut(s) 34, 158, 182, 193, 218, 245, 505, 1123
HspAI GCGC 2 cut(s) 302, 1250
KroI GCCGGC 1 cut(s) 970
KroNI GCCGGC 1 cut(s) 972
Ksp22I TGATCA 1 cut(s) 1299
Kzo9I GATC 5 cut(s) 54, 622, 1173, 1299, 1320
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 4 cut(s) 751, 817, 1074, 1203
LweI GCATC 2 cut(s) 6, 1264
MaeI CTAG 4 cut(s) 200, 321, 614, 848
MaeIII GTNAC 2 cut(s) 224, 1231
MalI GATC 5 cut(s) 56, 624, 1175, 1301, 1322
MboI GATC 5 cut(s) 54, 622, 1173, 1299, 1320
MboII GAAGA 7 cut(s) 94, 371, 499, 646, 662, 713, 1276
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 2 cut(s) 622, 1320
MhlI GDGCHC 2 cut(s) 255, 536
MluCI AATT 5 cut(s) 40, 234, 335, 768, 1162
MlyI GAGTC 1 cut(s) 282
MroNI GCCGGC 1 cut(s) 970
MseI TTAA 2 cut(s) 294, 927
MslI CAYNNNNRTG 1 cut(s) 875
MspI CCGG 5 cut(s) 661, 738, 744, 971, 1095
MspR9I CCNGG 3 cut(s) 310, 738, 1095
MunI CAATTG 1 cut(s) 768
MvaI CCWGG 1 cut(s) 310
MwoI GCNNNNNNNGC 8 cut(s) 259, 273, 310, 605, 614, 770, 1247, 1283
NaeI GCCGGC 1 cut(s) 972
NciI CCSGG 2 cut(s) 738, 1095
NcoI CCATGG 1 cut(s) 214
NdeII GATC 5 cut(s) 54, 622, 1173, 1299, 1320
NgoMIV GCCGGC 1 cut(s) 970
NlaIII CATG 8 cut(s) 34, 158, 182, 193, 218, 245, 505, 1123
NlaIV GGNNCC 4 cut(s) 624, 733, 742, 920
NmuCI GTSAC 2 cut(s) 224, 1231
NspI RCATGY 1 cut(s) 1123
NspV TTCGAA 2 cut(s) 944, 1146
PagI TCATGA 2 cut(s) 178, 189
PciI ACATGT 1 cut(s) 1119
PdiI GCCGGC 1 cut(s) 972
PfeI GAWTC 1 cut(s) 171
PflMI CCANNNNNTGG 1 cut(s) 247
PfoI TCCNGGA 1 cut(s) 1093
PinAI ACCGGT 2 cut(s) 660, 743
PkrI GCNGC 4 cut(s) 766, 807, 1089, 1218
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 918
PscI ACATGT 1 cut(s) 1119
Psp5II RGGWCCY 1 cut(s) 918
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 4 cut(s) 624, 733, 742, 920
PspPI GGNCC 3 cut(s) 658, 731, 918
PspPPI RGGWCCY 1 cut(s) 918
PsuI RGATCY 2 cut(s) 622, 1320
RsaI GTAC 2 cut(s) 117, 846
RsaNI GTAC 2 cut(s) 116, 845
RseI CAYNNNNRTG 1 cut(s) 875
SaqAI TTAA 2 cut(s) 294, 927
SatI GCNGC 4 cut(s) 765, 806, 1088, 1217
Sau3AI GATC 5 cut(s) 54, 622, 1173, 1299, 1320
Sau96I GGNCC 3 cut(s) 658, 731, 918
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 310, 738, 1095
SduI GDGCHC 2 cut(s) 255, 536
SfaNI GCATC 2 cut(s) 6, 1264
SfuI TTCGAA 2 cut(s) 944, 1146
SinI GGWCC 3 cut(s) 658, 731, 918
SmiMI CAYNNNNRTG 1 cut(s) 875
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SpeI ACTAGT 3 cut(s) 199, 320, 847
Sse9I AATT 5 cut(s) 40, 234, 335, 768, 1162
SsiI CCGC 1 cut(s) 897
SspMI CTAG 4 cut(s) 200, 321, 614, 848
StyD4I CCNGG 3 cut(s) 308, 736, 1093
StyI CCWWGG 5 cut(s) 214, 618, 688, 1152, 1291
TaaI ACNGT 1 cut(s) 681
TaqI TCGA 4 cut(s) 286, 641, 944, 1146
TaqII GACCGA 1 cut(s) 972
TasI AATT 5 cut(s) 40, 234, 335, 768, 1162
TatI WGTACW 1 cut(s) 844
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 2 cut(s) 294, 927
Tru9I TTAA 2 cut(s) 294, 927
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 2 cut(s) 224, 1231
TseI GCWGC 4 cut(s) 764, 805, 1087, 1216
Tsp45I GTSAC 2 cut(s) 224, 1231
TspDTI ATGAA 5 cut(s) 115, 167, 518, 991, 1277
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 247
VneI GTGCAC 1 cut(s) 532
VpaK11BI GGWCC 3 cut(s) 658, 731, 918
XapI RAATTY 1 cut(s) 234
XceI RCATGY 1 cut(s) 1123
XspI CTAG 4 cut(s) 200, 321, 614, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.