Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0017931

Physical Location & Seq

Genomic Coordinates Reverse (-)

16222565 .. 16225503

Gene Structure 2939 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032536

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGTGGAGTCGAGATTGGGGGACACCACCGACTAAGGCCAATTTAGTGTTTGACATAGATCGTCGGCCGCAATTGCTAGAAATCCCACATGGTAATCCAGTGCGCACGATGCAAGATGTTTCCCAGTTGCAGTGCCCACATTTGATCCCAGGACCAGGAGGTCCCCAACCTACCGGAGAAGGCGATCTTATAATCACTCCCTTTGCGCTTCTGCTTTTGGAAATCCCTCTGGCCAGGATTGCTGTTTTGCAGCGGACTAAATTTACCACGAAATCACAAAGGGGTGATTTTGGTGCATTGATGATACGTCAAGGACCTGAGGGGTGCGGCATCCTTGCAAATCCATTTCGATCAGACCTAATAATGGTCTCCACAGACTTCCACTTGAGTTCATTACAATTACCAGCACCAAACCCAGAACGCAGCGATAGCGAAGAGAGACAGTGTAAGCGATTGAAACCAGAGCAAGGTAGTTCATATACCCACAGATCAGTACTATCTCAGCTTCGTCCTTCATATGAATCTTTTCAAACTCCCAAGTCTTATGACACATCTAAGCTCCCCTCTGGGTGGTTAGATTGCTCATCGTTTGGTGAAGAGCTAGATTGCATGATACCTTCTAAAGTGCCACTAGGTGAATCCTACAATGTCTTTGTTCCTCATGGTAAGAGATACTCATTCAAGCAAGTGATTGAGGCACAGAGATGTCGGCGAAGAAAACTCGGTTTGGTTATCGATTTAACCAACACAACTCGTTACTATCGGACGTGTGATTTGGTGAAAATGGGTATCAAGCATGTCAGGATTCCATGCAATGGCCGGAATTCTGTACCGGACAACTTTTCAGTGAATCGTTTCGTATACGAGGTTATGCGATTCAAAGAGTACTACCCAGAGAAGTATATCCTTGTGCATTGTACACATGGACACAATCGCACAGGGTACATGATCATACATTACTTGATGCGGACGCAACCAATGAGGACAGTCACTCAAGCAATAAAAGATTTTGCTCAAGCGCGTCCTCCGGGAATATATAAACCTGACTACATCAATGCATTGTACTCATTCTATCATGAAATGAAGCCTGTGGAATTCGTTTGCCCACCTTCTCCTGAGTGGAAGAGATCTTCGGAATTTGTGCCAGACGACAATGATCAAATTTTGAAAAATAGTCATCAGGATAGGGATACAGATGGTGTAGTAATGACTAATGATGATATCCTGGGTGACGATATTCCTGATGACCAGCAAGAGGAACTGCGGAGGTTGTGCTACAAACTTCTTAAGGCCTTTCCCAGGGTAAGAGGAAGATGGCAATTCCCAGGGTCACACCCGGTTTCTCTTAACTGGGAGAATCTACAGTTGTTGAGGCAACAGTCTTACTATGCCACTTGGAAAGCTGATGGAACCCGTTATATGATGTTAATAACTTTACACGGATGTTACTTGATTGATAGAAGCTTCAATTTTAGGAGGGTTCAAATGAGGTTTCCCCGCAAACACTTAAATGGTTCTACTGAGAAGAGTACTCACCACTGCACGTTACTAGACGGGGAGATGGTGATCGATACTGAGCACCTGCCAGGTCCACAGAAGAAGCAGACCAGAAGATACCTTGTTTATGATATGATGGCACTCAACAACGGTTCTGTGGTAGAGTGGCCTTTTCAGGAGCGGTCGAAGAAGATTGAGGAGCAAGTAATTGAGCCTCGGACTAATGACCAGCGCCGAGTTTACGAAAGTAGGAGCAATAATCCTGACTACAGATATGACTTGGAACCATTCAGGGTGAGGATGAAGGGCTTTTGGCCACTCTCTAAGGTGAAGAAGATTTTGAAGGAGTTCATCCCAAAGCTTTCACATGATGCAGATGGCGTGATCTTTCAGGGTATGGACGATCCTTATGTTATCAGAAAACATGAACGCCTTGTGAAGTGGAAATACCCGGAAATGAACTCGGTTGATTTTCTCTTCGAGGTAGGTGGTGATGGTCGGCAACTGCTTTATCTGTATGCATATGGAGAAAAGCAACTAATGAAAGGAGAGAGGGTTGCATTTAAGGATAGTTCGGAACACTACTCTTTTTATTCGGGAAAAATAATAGAGTGTTTTCGGGATTCTGAGAAAAAAGAATGGGTCTACATGCGGAGCAGGACAGATAAATCGACTCCAAATGATGTAGAGACCTACAAAAAGGTAAAGAAAAGCATAGAGGACAATATCACGGAGGACGTCTTACTGAAGGAGATAGATAACATCATTCTTCTGCCTATGTATGTTGAGCAGCATGCAAATTCAGCACGGTTGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004484 GO:0004651 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006370 GO:0006396 GO:0006397 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008192 GO:0009452 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016071 GO:0016311 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016791 GO:0016817 GO:0016818 GO:0019222 GO:0034641 GO:0036260 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048518 GO:0050355 GO:0050789 GO:0060255 GO:0065007 GO:0070568 GO:0071704 GO:0090304 GO:0098501 GO:0098507 GO:0098518 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

DSPc mRNA_cap_C mRNA_cap_enzyme

Protein Analysis

772

Amino Acids

90.15

Weight (kDa)

8.92

Isoelectric Point (pI)

50.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	237 - 358	1.3e-10	Dual specificity phosphatase, catalytic domain
mRNA_cap_enzyme	PF01331	445 - 648	3.5e-63	mRNA capping enzyme, catalytic domain
mRNA_cap_C	PF03919	683 - 748	1.3e-16	mRNA capping enzyme, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01290
fragaria_vesca	FvH4_3g12521
rosa_chinensis	RchiOBHm_Chr5g0020531
rosa_laevigata	RLG00000032536
rosa_multiflora	Rmu_sc0009745.1_g000011 Rmu_sc0010439.1_g000007
rosa_rugosa	Rorug05G0060300
rosa_samantha	Rh5BG150300 Rh5CG161700 Rh5DG149600
rosa_wichuraiana	Rw5G013350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	191
AarI	CACCTGC	1 cut(s)	1590
AasI	GACNNNNNNGTC	1 cut(s)	159
AatII	GACGTC	1 cut(s)	2238
Acc16I	TGCGCA	1 cut(s)	104
Acc36I	ACCTGC	1 cut(s)	1590
AccB7I	CCANNNNNTGG	1 cut(s)	815
AccBSI	CCGCTC	1 cut(s)	1678
AccI	GTMKAC	2 cut(s)	861, 2142
AccII	CGCG	1 cut(s)	1021
AciI	CCGC	8 cut(s)	68, 253, 327, 967, 1264, 1498, 1678, 2149
AclWI	GGATC	2 cut(s)	139, 1895
AcoI	YGGCCR	4 cut(s)	65, 231, 817, 1811
AcsI	RAATTY	6 cut(s)	260, 823, 1094, 1136, 1161, 2296
AcuI	CTGAAG	1 cut(s)	2264
AcyI	GRCGYC	1 cut(s)	2235
AdeI	CACNNNGTG	1 cut(s)	635
AfaI	GTAC	7 cut(s)	495, 831, 887, 919, 944, 1064, 1531
AfiI	CCNNNNNNNGG	7 cut(s)	155, 364, 467, 570, 815, 1255, 1299
AflII	CTTAAG	1 cut(s)	1286
AflIII	ACRYGT	1 cut(s)	767
AgsI	TTSAA	8 cut(s)	457, 530, 682, 880, 1168, 1468, 1484, 1840
AjiI	CACGTC	1 cut(s)	768
AjnI	CCWGG	7 cut(s)	148, 154, 233, 1224, 1298, 1324, 1585
AluBI	AGCT	6 cut(s)	505, 559, 601, 1403, 1464, 1858
AluI	AGCT	6 cut(s)	505, 559, 601, 1403, 1464, 1858
Alw21I	GWGCWC	1 cut(s)	1581
Alw26I	GTCTC	3 cut(s)	373, 433, 2180
AlwI	GGATC	2 cut(s)	139, 1895
AoxI	GGCC	7 cut(s)	36, 65, 231, 817, 1290, 1664, 1811
ApeKI	GCWGC	3 cut(s)	250, 423, 2287
ApoI	RAATTY	6 cut(s)	260, 823, 1094, 1136, 1161, 2296
ArsI	GACNNNNNNTTYG	2 cut(s)	757, 789
Asp700I	GAANNNNTTC	3 cut(s)	525, 854, 1844
AspLEI	GCGC	4 cut(s)	105, 208, 1021, 1731
AspS9I	GGNCC	4 cut(s)	152, 161, 314, 1589
AsuC2I	CCSGG	3 cut(s)	1029, 1337, 1949
AvaII	GGWCC	4 cut(s)	152, 161, 314, 1589
AxyI	CCTNAGG	1 cut(s)	318
BaeGI	GKGCMC	1 cut(s)	137
BalI	TGGCCA	2 cut(s)	233, 1813
BarI	GAAGNNNNNNTAC	2 cut(s)	1928, 1960
Bbv12I	GWGCWC	1 cut(s)	1581
BbvI	GCAGC	3 cut(s)	262, 435, 2299
BccI	CCATC	7 cut(s)	1190, 1308, 1400, 1555, 1627, 1868, 1985
BciT130I	CCWGG	7 cut(s)	150, 156, 235, 1226, 1300, 1326, 1587
BciVI	GTATCC	1 cut(s)	1183
BclI	TGATCA	2 cut(s)	948, 1156
BcnI	CCSGG	3 cut(s)	1029, 1337, 1949
BcoDI	GTCTC	3 cut(s)	373, 433, 2180
BfaI	CTAG	4 cut(s)	77, 602, 632, 1550
BfmI	CTRYAG	2 cut(s)	1361, 1765
BfoI	RGCGCY	1 cut(s)	1732
BfrI	CTTAAG	1 cut(s)	1286
BfuAI	ACCTGC	1 cut(s)	1590
BfuI	GTATCC	1 cut(s)	1183
BglII	AGATCT	1 cut(s)	1127
BisI	GCNGC	5 cut(s)	68, 251, 328, 424, 2288
BlsI	GCNGC	5 cut(s)	69, 252, 329, 425, 2289
BmcAI	AGTACT	3 cut(s)	495, 887, 1531
Bme18I	GGWCC	4 cut(s)	152, 161, 314, 1589
BmgBI	CACGTC	1 cut(s)	768
BmgT120I	GGNCC	4 cut(s)	152, 161, 314, 1589
BmiI	GGNNCC	3 cut(s)	163, 1411, 1782
BmrI	ACTGGG	2 cut(s)	118, 1360
BmsI	GCATC	4 cut(s)	99, 339, 954, 1858
BmuI	ACTGGG	2 cut(s)	118, 1360
BpuEI	CTTGAG	3 cut(s)	406, 978, 999
BpuMI	CCSGG	3 cut(s)	1029, 1337, 1949
Bsa29I	ATCGAT	2 cut(s)	735, 1569
BsaBI	GATNNNNATC	1 cut(s)	1565
BsaHI	GRCGYC	1 cut(s)	2235
BsaI	GGTCTC	2 cut(s)	373, 2180
BsaJI	CCNNGG	7 cut(s)	148, 1225, 1298, 1299, 1324, 1325, 1712
BsaWI	WCCGGW	2 cut(s)	173, 832
BsaXI	ACNNNNNCTCC	2 cut(s)	2037, 2067
Bsc4I	CCNNNNNNNGG	7 cut(s)	155, 364, 467, 570, 815, 1255, 1299
Bse1I	ACTGG	3 cut(s)	98, 124, 1355
Bse21I	CCTNAGG	1 cut(s)	318
Bse3DI	GCAATG	1 cut(s)	820
Bse8I	GATNNNNATC	1 cut(s)	1565
BseBI	CCWGG	7 cut(s)	150, 156, 235, 1226, 1300, 1326, 1587
BseCI	ATCGAT	2 cut(s)	735, 1569
BseDI	CCNNGG	7 cut(s)	148, 1225, 1298, 1299, 1324, 1325, 1712
BseGI	GGATG	4 cut(s)	330, 1448, 1803, 1848
BseJI	GATNNNNATC	1 cut(s)	1565
BseLI	CCNNNNNNNGG	7 cut(s)	155, 364, 467, 570, 815, 1255, 1299
BseMI	GCAATG	1 cut(s)	820
BseMII	CTCAG	6 cut(s)	309, 515, 1107, 1512, 1566, 2115
BseNI	ACTGG	3 cut(s)	98, 124, 1355
BseRI	GAGGAG	1 cut(s)	1709
BseSI	GKGCMC	1 cut(s)	137
BseX3I	CGGCCG	1 cut(s)	65
BseXI	GCAGC	3 cut(s)	262, 435, 2299
BsgI	GTGCAG	1 cut(s)	1525
Bsh1236I	CGCG	1 cut(s)	1021
Bsh1285I	CGRYCG	2 cut(s)	68, 1682
BshFI	GGCC	7 cut(s)	38, 67, 233, 819, 1292, 1666, 1813
BshVI	ATCGAT	2 cut(s)	735, 1569
BsiEI	CGRYCG	2 cut(s)	68, 1682
BsiHKAI	GWGCWC	1 cut(s)	1581
BsiSI	CCGG	6 cut(s)	174, 820, 833, 1028, 1337, 1949
BslFI	GGGAC	2 cut(s)	34, 147
BslI	CCNNNNNNNGG	7 cut(s)	155, 364, 467, 570, 815, 1255, 1299
BsmAI	GTCTC	3 cut(s)	373, 433, 2180
BsmFI	GGGAC	2 cut(s)	34, 147
BsnI	GGCC	7 cut(s)	38, 67, 233, 819, 1292, 1666, 1813
Bso31I	GGTCTC	2 cut(s)	373, 2180
Bsp1286I	GDGCHC	2 cut(s)	137, 1581
Bsp1407I	TGTACA	1 cut(s)	917
BspACI	CCGC	8 cut(s)	68, 253, 327, 967, 1264, 1498, 1678, 2149
BspANI	GGCC	7 cut(s)	38, 67, 233, 819, 1292, 1666, 1813
BspCNI	CTCAG	6 cut(s)	310, 514, 1108, 1513, 1567, 2116
BspDI	ATCGAT	2 cut(s)	735, 1569
BspFNI	CGCG	1 cut(s)	1021
BspHI	TCATGA	1 cut(s)	1075
BspLI	GGNNCC	3 cut(s)	163, 1411, 1782
BspMI	ACCTGC	1 cut(s)	1590
BspPI	GGATC	2 cut(s)	139, 1895
BspQI	GCTCTTC	1 cut(s)	591
BspTI	CTTAAG	1 cut(s)	1286
BspTNI	GGTCTC	2 cut(s)	373, 2180
BsrBI	CCGCTC	1 cut(s)	1678
BsrDI	GCAATG	1 cut(s)	820
BsrGI	TGTACA	1 cut(s)	917
BsrI	ACTGG	3 cut(s)	98, 124, 1355
BssECI	CCNNGG	7 cut(s)	148, 1225, 1298, 1299, 1324, 1325, 1712
BssNAI	GTATAC	1 cut(s)	862
BssNI	GRCGYC	1 cut(s)	2235
Bst1107I	GTATAC	1 cut(s)	862
Bst2UI	CCWGG	7 cut(s)	150, 156, 235, 1226, 1300, 1326, 1587
Bst4CI	ACNGT	6 cut(s)	444, 988, 1365, 1380, 1649, 2307
Bst6I	CTCTTC	5 cut(s)	429, 591, 1118, 1520, 1979
BstACI	GRCGYC	1 cut(s)	2235
BstAFI	CTTAAG	1 cut(s)	1286
BstAUI	TGTACA	1 cut(s)	917
BstC8I	GCNNGC	1 cut(s)	2292
BstDEI	CTNAG	9 cut(s)	33, 318, 501, 555, 1116, 1521, 1575, 1821, 2124
BstENI	CCTNNNNNAGG	1 cut(s)	1297
BstF5I	GGATG	4 cut(s)	330, 1448, 1803, 1848
BstFNI	CGCG	1 cut(s)	1021
BstH2I	RGCGCY	1 cut(s)	1732
BstHHI	GCGC	4 cut(s)	105, 208, 1021, 1731
BstMAI	GTCTC	3 cut(s)	373, 433, 2180
BstMCI	CGRYCG	2 cut(s)	68, 1682
BstMWI	GCNNNNNNNGC	5 cut(s)	73, 109, 239, 429, 2300
BstNI	CCWGG	7 cut(s)	150, 156, 235, 1226, 1300, 1326, 1587
BstNSI	RCATGY	3 cut(s)	800, 2149, 2294
BstSFI	CTRYAG	2 cut(s)	1361, 1765
BstSLI	GKGCMC	1 cut(s)	137
BstUI	CGCG	1 cut(s)	1021
BstV1I	GCAGC	3 cut(s)	262, 435, 2299
BstX2I	RGATCY	1 cut(s)	1127
BstYI	RGATCY	1 cut(s)	1127
BstZ17I	GTATAC	1 cut(s)	862
BstZI	CGGCCG	1 cut(s)	65
Bsu15I	ATCGAT	2 cut(s)	735, 1569
Bsu36I	CCTNAGG	1 cut(s)	318
BsuI	GTATCC	1 cut(s)	1183
BsuRI	GGCC	7 cut(s)	38, 67, 233, 819, 1292, 1666, 1813
BsuTUI	ATCGAT	2 cut(s)	735, 1569
BtrI	CACGTC	1 cut(s)	768
BtsCI	GGATG	4 cut(s)	330, 1448, 1803, 1848
BtsI	GCAGTG	2 cut(s)	137, 1537
BtsIMutI	CAGTG	5 cut(s)	105, 137, 449, 852, 1537
BveI	ACCTGC	1 cut(s)	1590
Cac8I	GCNNGC	1 cut(s)	2292
CciI	TCATGA	1 cut(s)	1075
CfoI	GCGC	4 cut(s)	105, 208, 1021, 1731
Cfr13I	GGNCC	4 cut(s)	152, 161, 314, 1589
ClaI	ATCGAT	2 cut(s)	735, 1569
CseI	GACGC	2 cut(s)	979, 1010
Csp6I	GTAC	7 cut(s)	494, 830, 886, 918, 943, 1063, 1530
CviQI	GTAC	7 cut(s)	494, 830, 886, 918, 943, 1063, 1530
DdeI	CTNAG	9 cut(s)	33, 318, 501, 555, 1116, 1521, 1575, 1821, 2124
DraIII	CACNNNGTG	1 cut(s)	635
DrdI	GACNNNNNNGTC	1 cut(s)	159
DseDI	GACNNNNNNGTC	1 cut(s)	159
EaeI	YGGCCR	4 cut(s)	65, 231, 817, 1811
EagI	CGGCCG	1 cut(s)	65
Eam1104I	CTCTTC	5 cut(s)	429, 591, 1118, 1520, 1979
EarI	CTCTTC	5 cut(s)	429, 591, 1118, 1520, 1979
EclXI	CGGCCG	1 cut(s)	65
Eco147I	AGGCCT	1 cut(s)	1292
Eco31I	GGTCTC	2 cut(s)	373, 2180
Eco32I	GATATC	1 cut(s)	1222
Eco47I	GGWCC	4 cut(s)	152, 161, 314, 1589
Eco52I	CGGCCG	1 cut(s)	65
Eco57I	CTGAAG	1 cut(s)	2264
Eco81I	CCTNAGG	1 cut(s)	318
EcoNI	CCTNNNNNAGG	1 cut(s)	1297
EcoO109I	RGGNCCY	2 cut(s)	161, 314
EcoRI	GAATTC	2 cut(s)	823, 1094
EcoRII	CCWGG	7 cut(s)	148, 154, 233, 1224, 1298, 1324, 1585
EcoRV	GATATC	1 cut(s)	1222
EcoT22I	ATGCAT	2 cut(s)	1060, 2020
FalI	AAGNNNNNCTT	2 cut(s)	171, 203
FaqI	GGGAC	2 cut(s)	34, 147
FauI	CCCGC	1 cut(s)	1505
FauNDI	CATATG	2 cut(s)	517, 2020
FbaI	TGATCA	2 cut(s)	948, 1156
FblI	GTMKAC	2 cut(s)	861, 2142
Fnu4HI	GCNGC	5 cut(s)	68, 251, 328, 424, 2288
FokI	GGATG	4 cut(s)	317, 1455, 1810, 1835
Fsp4HI	GCNGC	5 cut(s)	68, 251, 328, 424, 2288
FspAI	RTGCGCAY	1 cut(s)	104
FspBI	CTAG	4 cut(s)	77, 602, 632, 1550
FspI	TGCGCA	1 cut(s)	104
GlaI	GCGC	4 cut(s)	104, 207, 1020, 1730
GluI	GCNGC	5 cut(s)	68, 251, 328, 424, 2288
HaeII	RGCGCY	1 cut(s)	1732
HaeIII	GGCC	7 cut(s)	38, 67, 233, 819, 1292, 1666, 1813
HapII	CCGG	6 cut(s)	174, 820, 833, 1028, 1337, 1949
HgaI	GACGC	2 cut(s)	979, 1010
HhaI	GCGC	4 cut(s)	105, 208, 1021, 1731
Hin1I	GRCGYC	1 cut(s)	2235
Hin6I	GCGC	4 cut(s)	103, 206, 1019, 1729
HinP1I	GCGC	4 cut(s)	103, 206, 1019, 1729
HindIII	AAGCTT	2 cut(s)	1462, 1856
HinfI	GANTC	9 cut(s)	7, 521, 638, 805, 850, 876, 1357, 2120, 2170
HpaII	CCGG	6 cut(s)	174, 820, 833, 1028, 1337, 1949
Hpy166II	GTNNAC	5 cut(s)	862, 920, 1592, 1738, 2143
Hpy188I	TCNGA	7 cut(s)	355, 765, 1135, 1716, 1916, 2074, 2125
Hpy8I	GTNNAC	5 cut(s)	862, 920, 1592, 1738, 2143
Hpy99I	CGWCG	1 cut(s)	66
HpyAV	CCTTC	7 cut(s)	173, 522, 627, 1119, 1795, 1834, 2239
HpyCH4III	ACNGT	6 cut(s)	444, 988, 1365, 1380, 1649, 2307
HpyCH4IV	ACGT	4 cut(s)	307, 767, 1544, 2235
HpyF10VI	GCNNNNNNNGC	5 cut(s)	73, 109, 239, 429, 2300
HpyF3I	CTNAG	9 cut(s)	33, 318, 501, 555, 1116, 1521, 1575, 1821, 2124
HpySE526I	ACGT	4 cut(s)	307, 767, 1544, 2235
Hsp92I	GRCGYC	1 cut(s)	2235
HspAI	GCGC	4 cut(s)	103, 206, 1019, 1729
Ksp22I	TGATCA	2 cut(s)	948, 1156
LguI	GCTCTTC	1 cut(s)	591
LmnI	GCTCC	5 cut(s)	564, 1675, 1696, 1749, 2151
Lsp1109I	GCAGC	3 cut(s)	262, 435, 2299
LweI	GCATC	4 cut(s)	99, 339, 954, 1858
MaeI	CTAG	4 cut(s)	77, 602, 632, 1550
MaeII	ACGT	4 cut(s)	307, 767, 1544, 2235
MaeIII	GTNAC	6 cut(s)	755, 988, 1229, 1329, 1445, 1545
MbiI	CCGCTC	1 cut(s)	1678
MfeI	CAATTG	1 cut(s)	71
MflI	RGATCY	1 cut(s)	1127
MhlI	GDGCHC	2 cut(s)	137, 1581
MlsI	TGGCCA	2 cut(s)	233, 1813
MluNI	TGGCCA	2 cut(s)	233, 1813
MlyI	GAGTC	2 cut(s)	16, 2164
Mox20I	TGGCCA	2 cut(s)	233, 1813
Mph1103I	ATGCAT	2 cut(s)	1060, 2020
MroXI	GAANNNNTTC	3 cut(s)	525, 854, 1844
MscI	TGGCCA	2 cut(s)	233, 1813
MseI	TTAA	6 cut(s)	740, 1287, 1347, 1427, 1508, 2061
MslI	CAYNNNNRTG	2 cut(s)	703, 1509
Msp20I	TGGCCA	2 cut(s)	233, 1813
MspA1I	CMGCKG	1 cut(s)	253
MspCI	CTTAAG	1 cut(s)	1286
MspI	CCGG	6 cut(s)	174, 820, 833, 1028, 1337, 1949
MunI	CAATTG	1 cut(s)	71
MvaI	CCWGG	7 cut(s)	150, 156, 235, 1226, 1300, 1326, 1587
MvnI	CGCG	1 cut(s)	1021
MwoI	GCNNNNNNNGC	5 cut(s)	73, 109, 239, 429, 2300
NciI	CCSGG	3 cut(s)	1029, 1337, 1949
NdeI	CATATG	2 cut(s)	517, 2020
NlaIV	GGNNCC	3 cut(s)	163, 1411, 1782
NmeAIII	GCCGAG	1 cut(s)	1757
NmuCI	GTSAC	3 cut(s)	988, 1229, 1329
NsbI	TGCGCA	1 cut(s)	104
NsiI	ATGCAT	2 cut(s)	1060, 2020
NspI	RCATGY	3 cut(s)	800, 2149, 2294
PaeI	GCATGC	1 cut(s)	2294
PagI	TCATGA	1 cut(s)	1075
PaqCI	CACCTGC	1 cut(s)	1590
PasI	CCCWGGG	2 cut(s)	1299, 1325
PceI	AGGCCT	1 cut(s)	1292
PciSI	GCTCTTC	1 cut(s)	591
PdmI	GAANNNNTTC	3 cut(s)	525, 854, 1844
PfeI	GAWTC	7 cut(s)	521, 638, 805, 850, 876, 1357, 2120
PflMI	CCANNNNNTGG	1 cut(s)	815
PfoI	TCCNGGA	1 cut(s)	1027
PkrI	GCNGC	5 cut(s)	69, 252, 329, 425, 2289
PleI	GAGTC	2 cut(s)	15, 2164
PpsI	GAGTC	2 cut(s)	15, 2164
PpuMI	RGGWCCY	2 cut(s)	161, 314
PsiI	TTATAA	1 cut(s)	191
Psp5II	RGGWCCY	2 cut(s)	161, 314
Psp6I	CCWGG	7 cut(s)	148, 154, 233, 1224, 1298, 1324, 1585
PspGI	CCWGG	7 cut(s)	148, 154, 233, 1224, 1298, 1324, 1585
PspN4I	GGNNCC	3 cut(s)	163, 1411, 1782
PspPI	GGNCC	4 cut(s)	152, 161, 314, 1589
PspPPI	RGGWCCY	2 cut(s)	161, 314
PsuI	RGATCY	1 cut(s)	1127
RsaI	GTAC	7 cut(s)	495, 831, 887, 919, 944, 1064, 1531
RsaNI	GTAC	7 cut(s)	494, 830, 886, 918, 943, 1063, 1530
RseI	CAYNNNNRTG	2 cut(s)	703, 1509
SapI	GCTCTTC	1 cut(s)	591
SaqAI	TTAA	6 cut(s)	740, 1287, 1347, 1427, 1508, 2061
SatI	GCNGC	5 cut(s)	68, 251, 328, 424, 2288
Sau96I	GGNCC	4 cut(s)	152, 161, 314, 1589
ScaI	AGTACT	3 cut(s)	495, 887, 1531
SchI	GAGTC	2 cut(s)	16, 2164
SduI	GDGCHC	2 cut(s)	137, 1581
SfaNI	GCATC	4 cut(s)	99, 339, 954, 1858
SfcI	CTRYAG	2 cut(s)	1361, 1765
SinI	GGWCC	4 cut(s)	152, 161, 314, 1589
SmiMI	CAYNNNNRTG	2 cut(s)	703, 1509
SmlI	CTYRAG	4 cut(s)	385, 993, 1014, 1286
SmoI	CTYRAG	4 cut(s)	385, 993, 1014, 1286
SphI	GCATGC	1 cut(s)	2294
SseBI	AGGCCT	1 cut(s)	1292
SsiI	CCGC	8 cut(s)	68, 253, 327, 967, 1264, 1498, 1678, 2149
SspMI	CTAG	4 cut(s)	77, 602, 632, 1550
StuI	AGGCCT	1 cut(s)	1292
TaaI	ACNGT	6 cut(s)	444, 988, 1365, 1380, 1649, 2307
TaiI	ACGT	4 cut(s)	310, 770, 1547, 2238
TaqI	TCGA	7 cut(s)	10, 349, 735, 1569, 1682, 1977, 2168
TatI	WGTACW	5 cut(s)	493, 885, 917, 1062, 1529
TauI	GCSGC	2 cut(s)	70, 330
TfiI	GAWTC	7 cut(s)	521, 638, 805, 850, 876, 1357, 2120
Tru1I	TTAA	6 cut(s)	740, 1287, 1347, 1427, 1508, 2061
Tru9I	TTAA	6 cut(s)	740, 1287, 1347, 1427, 1508, 2061
TscAI	CASTG	5 cut(s)	105, 137, 449, 852, 1544
TseFI	GTSAC	3 cut(s)	988, 1229, 1329
TseI	GCWGC	3 cut(s)	250, 423, 2287
Tsp45I	GTSAC	3 cut(s)	988, 1229, 1329
TspGWI	ACGGA	2 cut(s)	1455, 2243
TspRI	CASTG	5 cut(s)	105, 137, 449, 852, 1544
Van91I	CCANNNNNTGG	1 cut(s)	815
Vha464I	CTTAAG	1 cut(s)	1286
VpaK11BI	GGWCC	4 cut(s)	152, 161, 314, 1589
XagI	CCTNNNNNAGG	1 cut(s)	1297
XapI	RAATTY	6 cut(s)	260, 823, 1094, 1136, 1161, 2296
XceI	RCATGY	3 cut(s)	800, 2149, 2294
XmiI	GTMKAC	2 cut(s)	861, 2142
XmnI	GAANNNNTTC	3 cut(s)	525, 854, 1844
XspI	CTAG	4 cut(s)	77, 602, 632, 1550
ZraI	GACGTC	1 cut(s)	2236
ZrmI	AGTACT	3 cut(s)	495, 887, 1531
Zsp2I	ATGCAT	2 cut(s)	1060, 2020

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.