RLG00000032588

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0020312
Physical Location & Seq
Forward (+)
16822124 .. 16823145
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032588

Sequence Viewer

Length: 456 bp
ATGGGGTTTCTGCAAGTTAGGGATCTATCAAAGAATCAACTGACTGGCTCATTACCAGACTTGGGGAGCTTGCGAAACCTTCGGTATCTATATCTACAGTACAACTTGCTGGAAGGCGCTATACCACCAACCTTGGGTTCACTAGTGAATCTTCGGCAACTGGATATTTCTGAGAACCAGCTCACTGGTCCGCTTCCCAAAACACTTGGAAATTTGAAATCCCTCAGCGGCTTCTGGGCCATAGCCAATTCCTTGAATGATAAATTTCCGGACACTTATGGGAACCTTACGAGTCTAAAATATTTTTCAATATCCGGGAATTATATCTCTGGTCCATTCCCAGTTGACATCATAAAGAACTGGACTAATATTGAAACTCTGTCACTCGTGGGAAACAATTTCAAAGGAAATTTGGCTAAAGAAGTATTCAATTTGCCAGAACTTCGGTATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COR LRR_4 LRR_8 MBT Roc TIR_2

Protein Analysis

152

Amino Acids

16.83

Weight (kDa)

8.86

Isoelectric Point (pI)

19.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 13 - 151 3e-12 Leucine-rich repeat region
LRR_8 PF13855 25 - 75 4.7e-06 Leucine rich repeat
LRR_4 PF12799 26 - 62 4.3e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020312)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37760
malus_domestica MD09G1116000.v1.1
rosa_laevigata RLG00000009836 RLG00000032588
rosa_multiflora Rmu_co7997308.1_g000001 Rmu_sc0008509.1_g000027

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 268
AciI CCGC 2 cut(s) 191, 228
AclWI GGATC 1 cut(s) 30
AcsI RAATTY 3 cut(s) 211, 263, 409
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 2 cut(s) 62, 134
AgsI TTSAA 6 cut(s) 217, 256, 309, 374, 403, 430
AhlI ACTAGT 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 121, 153
AluBI AGCT 2 cut(s) 69, 181
AluI AGCT 2 cut(s) 69, 181
AlwI GGATC 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 1 cut(s) 237
ApoI RAATTY 3 cut(s) 211, 263, 409
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 3 cut(s) 188, 237, 332
AsuC2I CCSGG 1 cut(s) 316
AvaII GGWCC 2 cut(s) 188, 332
BaeI ACNNNNGTAYC 1 cut(s) 431
BarI GAAGNNNNNNTAC 2 cut(s) 105, 137
BauI CACGAG 1 cut(s) 386
BbvCI CCTCAGC 1 cut(s) 224
BcnI CCSGG 1 cut(s) 316
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 95
BfoI RGCGCY 1 cut(s) 120
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 2 cut(s) 188, 332
BmgT120I GGNCC 3 cut(s) 188, 237, 332
BmiI GGNNCC 1 cut(s) 284
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 335
BmuI ACTGGG 1 cut(s) 335
Bpu10I CCTNAGC 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 134
Bse1I ACTGG 5 cut(s) 49, 165, 190, 341, 365
BseAI TCCGGA 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 62, 134
BseMII CTCAG 2 cut(s) 162, 238
BseNI ACTGG 5 cut(s) 49, 165, 190, 341, 365
BshFI GGCC 1 cut(s) 239
BsiSI CCGG 2 cut(s) 269, 315
BslI CCNNNNNNNGG 2 cut(s) 62, 134
BsnI GGCC 1 cut(s) 239
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 2 cut(s) 191, 228
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 2 cut(s) 163, 237
BspEI TCCGGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 284
BspPI GGATC 1 cut(s) 30
BsrI ACTGG 5 cut(s) 49, 165, 190, 341, 365
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 22
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 2 cut(s) 171, 224
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 314
BstSFI CTRYAG 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 1 cut(s) 22
BsuRI GGCC 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 71
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 188, 237, 332
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviJI RGCY 7 cut(s) 48, 69, 181, 231, 239, 245, 416
CviKI_1 RGCY 7 cut(s) 48, 69, 181, 231, 239, 245, 416
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 171, 224
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 188, 332
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaiI YATR 6 cut(s) 91, 122, 242, 279, 324, 353
Fnu4HI GCNGC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 229
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 118
GluI GCNGC 1 cut(s) 229
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 1 cut(s) 239
HapII CCGG 2 cut(s) 269, 315
HhaI GCGC 1 cut(s) 119
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 3 cut(s) 34, 148, 292
HpaII CCGG 2 cut(s) 269, 315
Hpy166II GTNNAC 2 cut(s) 140, 346
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 1 cut(s) 269
Hpy8I GTNNAC 2 cut(s) 140, 346
HpyAV CCTTC 2 cut(s) 89, 107
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 13
HpyF3I CTNAG 2 cut(s) 171, 224
HspAI GCGC 1 cut(s) 117
Kpn2I TCCGGA 1 cut(s) 268
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 66
MaeI CTAG 1 cut(s) 143
MaeIII GTNAC 1 cut(s) 381
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 1 cut(s) 143
MflI RGATCY 1 cut(s) 22
MluCI AATT 7 cut(s) 211, 247, 263, 319, 397, 409, 430
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 1 cut(s) 233
MroI TCCGGA 1 cut(s) 268
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 2 cut(s) 269, 315
MspR9I CCNGG 1 cut(s) 316
NciI CCSGG 1 cut(s) 316
NdeII GATC 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 284
NmuCI GTSAC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 34, 148
PfoI TCCNGGA 1 cut(s) 314
PkrI GCNGC 1 cut(s) 230
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 284
PspPI GGNCC 3 cut(s) 188, 237, 332
PsuI RGATCY 1 cut(s) 22
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 3 cut(s) 188, 237, 332
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 316
SetI ASST 5 cut(s) 71, 81, 134, 183, 288
SfcI CTRYAG 1 cut(s) 95
SinI GGWCC 2 cut(s) 188, 332
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 7 cut(s) 211, 247, 263, 319, 397, 409, 430
SsiI CCGC 2 cut(s) 191, 228
SspI AATATT 2 cut(s) 302, 370
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 99
TasI AATT 7 cut(s) 211, 247, 263, 319, 397, 409, 430
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 2 cut(s) 34, 148
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 381
Tsp45I GTSAC 1 cut(s) 381
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 188, 332
XapI RAATTY 3 cut(s) 211, 263, 409
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.