RLG00000034263

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017741
Physical Location & Seq
Forward (+)
39605984 .. 39609736
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034263

Sequence Viewer

Length: 2313 bp
ATGGAGGAGGCGCCGATCGAGTGGTTCTGCGTCGTCGGAGAAGGCGCCGATGAAGACCGAAACAGAGGAGGTGTTTTCCTCGGAAACCTTATCTTCCGGCAACGGAGCAATCCGGGTCGGGTCCGACACCGGGCTTATGGAAGGCCCGTCGTTCACGAACCGGGCGTGTTCTCTGGATATGGTTTCGTTGTGGGGGAAAGGTTCAGTTTTTCCGAGTGCACCATGACAATCACCGCAGTCTCTCCCTTAATGCTTGTCACCCAAACCCTCCCATTGTTAGAGAAATGCAAAACCATAACTGAACTGCGCCAGCTTCACGCCCACATAGTCAAATCAAACCTCGTCCACCACACATCAACCATAACCAAACTCATTTCCCTTTGCTCCCACCATGGTGGCCTAGACCATGCTCTTTCAGTCTTTCATCGAATCCCGGACCCCAACCCCTTCATCTTTTTCTCACTCATAAAGCAAATGTCCGAGAGTTCAAGTCCAACAGAAGCGGTACTCTTCTATGCTCACATGCTCTCTTCTTTAGAAAATTTGAACGGGGTCGAGTTTTTGCTCCCTGCATTGCTCAAAGCGTGCAGCAAGTCACGTTCTTTGGAGGAGGGGAGGCAAGTTCATGGCCAGATATTGAAAACCCAGATGTTGTTTGATCCGTATGTTGCGAATGCATTGATTAGGATGTACTCGGAGCTGGGTTGTATTGGATTGGCACACAGGGTGTTTGATAAAATGGTTCAGAGAGACCAAGTTTCTTGGAATTCACTGATTACTGGTTATTTGAGGGTCGGGGAGGTTGAGTTGGCTCGTGGGATTTTTAATGAGGTTCCGGAGAGGGACTTGGTTTCGTCTAATGCAATGATTGATGGGTATGGGAAGAGTGGCAAGTGTGAGCTTGCTGAGGAGGTTTTTAGGACAATGCATCATAAAGATGTGGTTACTTGGACGTCAATGGTTTCAGGGTATGTATTCAATCACCGTCCGAAGGAAGCATTGGGTTTGTTTAGGGAAATGTTGAGTCAGGGCATTCGGCCTGATGCCCCGGCTCTTGTTAGTGTTCTCTCAGCCATTGCTGACTTGGGTTTCGTTGAGGAAGGCAAATGGGTGCATGCTTATATTTGTACGAATAAGATTAGGTTGAGTTCAGGGTTTCTTGGATCAGCTCTTATAGATATGTATGCTAAATGTGGGCATATAGAGAATGCTTACCATGTCTTTGAAAACATAGCTCATTATAGGAACATTGGGGACTGGAATTGCATGATCTCTGGCCTTGCTATGCATGGCCTAGGTCATGAAGCGCTGGACATTTTCCTTGACATGAAAATGAAAGAAATTGAGCCTGATGAGATCACTTTCTTAGGACTTCTTACTGCTTGTAACCATGGAGGTCTAGTTGATGAAGGTAGATACTGTTTTGAACTCATGCAGGAGAAGTACGGACTTGTTCCTAAGATTCAGCATTATGGATGCATTATTGATCTTTTAGGAAGAGCAGGGCACTTGGAGGAGGCGGTTGACGTTATACAGAATATGCCCATAGAGGCGGATGCTATAGTTTGGAAGGCAATTCTCAGCGCTTGCACGATTCATGGTAATGTTGCAATTGGGGAAAGTGCTGCCCTCAAGGCAATAGATTTGGCTCCATACGACTCAAGTTGTTACATTCTCCTCTCGAATCTTTATGCCAAAGTTGGGAGATGGGATGATGTGGCCAATGTTAGATTGATGATGAAACAGAGGGGAGTTCGAAAGATTCCAGGGTCAAGTTCAGTACTCGTGAATGGAAAGGTTCATGAATTCCTTGTGGGGAAGGAAATGAATGTTAAATACAGTGTTGAGGTTCGTACCAAGATAGAGGAGGTTGTCTCTAGGTTGAAATTGGAAGGATACAAACCTGATTTGTCTCAAGTCCTTTTGGATATTGAAGAGGAAAGCAAAGAGGACTCACTTATTCTACACAGCGAGAAGATGGCCCTTGCATTTGGACTAATAAACACAAGCAAGGGTTCTCCTATTCACATAGTGAAGAACTTAAGGATTTGTTGTGATTGCCATTCCTTTATCAAGCTGGTTTCTGGAGTTTATAATTGTCGAATTGTTGTGAGAGACCAAAACCGTTTCCATCAATTTGAGAATGTCTATGAAGCTGTAAAGCTACATCATCCTGTGGAAGAATCGAGCTCAATGGGCAGGTTTCAGTCTCAATCTTTGGCATTAGGAATGCAGGCTACACTAGCATCTCTTCAAATTCCCCTGGAAAGGAGGGTGGCTCGATTATCACTAATATGCGCATTTAGAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR

Protein Analysis

771

Amino Acids

86.06

Weight (kDa)

6.5

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 284 - 313 4.1e-06 PPR repeat
PPR_2 PF13041 312 - 349 1.1e-08 PPR repeat family
PPR PF01535 315 - 345 1.8e-07 PPR repeat
PPR_2 PF13041 420 - 462 2.1e-09 PPR repeat family
E_motif PF20431 533 - 594 2.2e-17 E motif
DYW_deaminase PF14432 632 - 715 1.3e-31 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017741)

Species Orthologous Gene IDs
malus_domestica MD11G1163700.v1.1
pyrus_communis pycom11g13520
rosa_chinensis RchiOBHm_Chr5g0044291
rosa_laevigata RLG00000034263
rosa_multiflora Rmu_sc0015666.1_g000004
rosa_roxburghii Rroxscaffold_1G00036670
rosa_rugosa Rorug05G0212900
rosa_samantha Rh5AG297500 Rh5BG304800 Rh5CG331700 Rh5DG314000
rosa_wichuraiana Rw5G027530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2094
AatII GACGTC 1 cut(s) 956
Acc16I TGCGCA 1 cut(s) 2299
Acc36I ACCTGC 1 cut(s) 2190
AccB1I GGYRCC 2 cut(s) 10, 44
AccIII TCCGGA 1 cut(s) 835
AciI CCGC 4 cut(s) 234, 503, 1520, 1553
AclWI GGATC 2 cut(s) 653, 1171
AcoI YGGCCR 2 cut(s) 628, 1719
AcsI RAATTY 4 cut(s) 541, 766, 1805, 2256
AcyI GRCGYC 3 cut(s) 11, 45, 953
AdeI CACNNNGTG 2 cut(s) 727, 2032
AfaI GTAC 6 cut(s) 507, 692, 1129, 1445, 1782, 1855
AfeI AGCGCT 2 cut(s) 1308, 1585
AfiI CCNNNNNNNGG 4 cut(s) 130, 991, 1701, 2268
AflII CTTAAG 1 cut(s) 2041
AgsI TTSAA 9 cut(s) 489, 547, 640, 979, 1226, 1427, 1885, 1934, 2255
AjnI CCWGG 2 cut(s) 1765, 2262
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
AleI CACNNNNGTG 1 cut(s) 393
AluBI AGCT 9 cut(s) 313, 700, 901, 1169, 1235, 2077, 2156, 2164, 2190
AluI AGCT 9 cut(s) 313, 700, 901, 1169, 1235, 2077, 2156, 2164, 2190
Alw21I GWGCWC 2 cut(s) 221, 2192
Alw26I GTCTC 6 cut(s) 244, 744, 1879, 1917, 2109, 2214
Alw44I GTGCAC 1 cut(s) 217
AlwI GGATC 2 cut(s) 653, 1171
Aor13HI TCCGGA 1 cut(s) 835
Aor51HI AGCGCT 2 cut(s) 1308, 1585
AoxI GGCC 8 cut(s) 143, 397, 628, 1037, 1276, 1291, 1719, 1980
ApaLI GTGCAC 1 cut(s) 217
ApeKI GCWGC 2 cut(s) 588, 1625
ApoI RAATTY 4 cut(s) 541, 766, 1805, 2256
ArsI GACNNNNNNTTYG 2 cut(s) 1073, 1105
AspA2I CCTAGG 1 cut(s) 1294
AspLEI GCGC 6 cut(s) 13, 47, 309, 1309, 1586, 2300
AspS9I GGNCC 4 cut(s) 121, 144, 436, 1981
AsuC2I CCSGG 5 cut(s) 114, 131, 162, 434, 1049
AsuHPI GGTGA 3 cut(s) 223, 250, 974
AsuII TTCGAA 1 cut(s) 1756
AvaII GGWCC 2 cut(s) 121, 436
AvrII CCTAGG 1 cut(s) 1294
BaeGI GKGCMC 2 cut(s) 221, 1509
BalI TGGCCA 2 cut(s) 630, 1721
BanI GGYRCC 2 cut(s) 10, 44
BanII GRGCYC 1 cut(s) 2192
BauI CACGAG 2 cut(s) 813, 1784
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 221, 2192
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 2 cut(s) 600, 1612
BccI CCATC 4 cut(s) 866, 1701, 1972, 2139
BciT130I CCWGG 2 cut(s) 1767, 2264
BciVI GTATCC 1 cut(s) 1889
BcnI CCSGG 5 cut(s) 114, 131, 162, 434, 1049
BcoDI GTCTC 6 cut(s) 244, 744, 1879, 1917, 2109, 2214
BfaI CTAG 5 cut(s) 401, 1295, 1400, 1878, 2243
BfmI CTRYAG 1 cut(s) 1560
BfoI RGCGCY 4 cut(s) 14, 48, 1310, 1587
BfrI CTTAAG 1 cut(s) 2041
BfuAI ACCTGC 1 cut(s) 2190
BfuI GTATCC 1 cut(s) 1889
BglI GCCNNNNNGGC 1 cut(s) 1634
BisI GCNGC 2 cut(s) 589, 1626
BlnI CCTAGG 1 cut(s) 1294
BlsI GCNGC 2 cut(s) 590, 1627
BmcAI AGTACT 1 cut(s) 1782
Bme1390I CCNGG 7 cut(s) 114, 131, 162, 434, 1049, 1767, 2264
Bme18I GGWCC 2 cut(s) 121, 436
BmgT120I GGNCC 4 cut(s) 121, 144, 436, 1981
BmiI GGNNCC 6 cut(s) 12, 46, 122, 438, 834, 1650
BmrFI CCNGG 7 cut(s) 114, 131, 162, 434, 1049, 1767, 2264
BmsI GCATC 5 cut(s) 937, 1033, 1466, 1546, 2255
BpiI GAAGAC 1 cut(s) 60
BplI GAGNNNNNCTC 4 cut(s) 1859, 1891, 2263, 2295
BpmI CTGGAG 1 cut(s) 2106
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 1756
BpuEI CTTGAG 3 cut(s) 1616, 1645, 1899
BpuMI CCSGG 5 cut(s) 114, 131, 162, 434, 1049
BsaHI GRCGYC 3 cut(s) 11, 45, 953
BsaI GGTCTC 2 cut(s) 744, 2109
BsaJI CCNNGG 7 cut(s) 79, 391, 1047, 1294, 1390, 1766, 2262
BsaWI WCCGGW 1 cut(s) 835
BsaXI ACNNNNNCTCC 4 cut(s) 791, 821, 1386, 1416
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 991, 1701, 2268
Bse1I ACTGG 2 cut(s) 784, 1262
Bse3DI GCAATG 3 cut(s) 572, 870, 1074
BseAI TCCGGA 1 cut(s) 835
BseBI CCWGG 2 cut(s) 1767, 2264
BseDI CCNNGG 7 cut(s) 79, 391, 1047, 1294, 1390, 1766, 2262
BseGI GGATG 5 cut(s) 693, 1481, 1561, 1717, 2170
BseLI CCNNNNNNNGG 4 cut(s) 130, 991, 1701, 2268
BseMI GCAATG 3 cut(s) 572, 870, 1074
BseMII CTCAG 3 cut(s) 897, 1083, 1594
BseNI ACTGG 2 cut(s) 784, 1262
BseRI GAGGAG 7 cut(s) 20, 81, 623, 923, 1529, 1667, 1880
BseSI GKGCMC 2 cut(s) 221, 1509
BseXI GCAGC 2 cut(s) 600, 1612
BseYI CCCAGC 1 cut(s) 700
BsgI GTGCAG 1 cut(s) 607
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 8 cut(s) 145, 399, 630, 1039, 1278, 1293, 1721, 1982
BshNI GGYRCC 2 cut(s) 10, 44
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 2 cut(s) 221, 2192
BsiSI CCGG 7 cut(s) 97, 113, 130, 161, 434, 836, 1049
BslFI GGGAC 2 cut(s) 857, 1268
BslI CCNNNNNNNGG 4 cut(s) 130, 991, 1701, 2268
BsmAI GTCTC 6 cut(s) 244, 744, 1879, 1917, 2109, 2214
BsmFI GGGAC 2 cut(s) 857, 1268
BsmI GAATGC 4 cut(s) 679, 1032, 1213, 2235
BsnI GGCC 8 cut(s) 145, 399, 630, 1039, 1278, 1293, 1721, 1982
Bso31I GGTCTC 2 cut(s) 744, 2109
Bsp119I TTCGAA 1 cut(s) 1756
Bsp1286I GDGCHC 3 cut(s) 221, 1509, 2192
Bsp13I TCCGGA 1 cut(s) 835
Bsp143I GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
Bsp19I CCATGG 2 cut(s) 391, 1390
BspACI CCGC 4 cut(s) 234, 503, 1520, 1553
BspANI GGCC 8 cut(s) 145, 399, 630, 1039, 1278, 1293, 1721, 1982
BspCNI CTCAG 3 cut(s) 898, 1082, 1593
BspEI TCCGGA 1 cut(s) 835
BspHI TCATGA 2 cut(s) 1300, 1801
BspLI GGNNCC 6 cut(s) 12, 46, 122, 438, 834, 1650
BspMI ACCTGC 1 cut(s) 2190
BspPI GGATC 2 cut(s) 653, 1171
BspQI GCTCTTC 1 cut(s) 1492
BspT104I TTCGAA 1 cut(s) 1756
BspT107I GGYRCC 2 cut(s) 10, 44
BspTI CTTAAG 1 cut(s) 2041
BspTNI GGTCTC 2 cut(s) 744, 2109
BsrDI GCAATG 3 cut(s) 572, 870, 1074
BsrI ACTGG 2 cut(s) 784, 1262
BssECI CCNNGG 7 cut(s) 79, 391, 1047, 1294, 1390, 1766, 2262
BssMI GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
BssNI GRCGYC 3 cut(s) 11, 45, 953
BssSI CACGAG 2 cut(s) 813, 1784
BssT1I CCWWGG 3 cut(s) 391, 1294, 1390
Bst2BI CACGAG 2 cut(s) 813, 1784
Bst2UI CCWGG 2 cut(s) 1767, 2264
Bst4CI ACNGT 4 cut(s) 986, 1421, 1841, 2126
Bst6I CTCTTC 6 cut(s) 515, 535, 878, 1492, 1929, 2256
BstACI GRCGYC 3 cut(s) 11, 45, 953
BstAFI CTTAAG 1 cut(s) 2041
BstBI TTCGAA 1 cut(s) 1756
BstC8I GCNNGC 6 cut(s) 311, 586, 903, 1116, 1588, 2235
BstDEI CTNAG 5 cut(s) 906, 1069, 1366, 1458, 1580
BstDSI CCRYGG 2 cut(s) 391, 1390
BstF5I GGATG 5 cut(s) 693, 1481, 1561, 1717, 2170
BstH2I RGCGCY 4 cut(s) 14, 48, 1310, 1587
BstHHI GCGC 6 cut(s) 13, 47, 309, 1309, 1586, 2300
BstKTI GATC 6 cut(s) 18, 661, 1166, 1272, 1359, 1489
BstMAI GTCTC 6 cut(s) 244, 744, 1879, 1917, 2109, 2214
BstMBI GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 3 cut(s) 1634, 2196, 2243
BstNI CCWGG 2 cut(s) 1767, 2264
BstNSI RCATGY 2 cut(s) 526, 1118
BstSCI CCNGG 7 cut(s) 112, 129, 160, 432, 1047, 1765, 2262
BstSFI CTRYAG 1 cut(s) 1560
BstSLI GKGCMC 2 cut(s) 221, 1509
BstV1I GCAGC 2 cut(s) 600, 1612
BstV2I GAAGAC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 395
BsuI GTATCC 1 cut(s) 1889
BsuRI GGCC 8 cut(s) 145, 399, 630, 1039, 1278, 1293, 1721, 1982
BtgI CCRYGG 2 cut(s) 391, 1390
BtsCI GGATG 5 cut(s) 693, 1481, 1561, 1717, 2170
BtsIMutI CAGTG 2 cut(s) 770, 1846
BveI ACCTGC 1 cut(s) 2190
Cac8I GCNNGC 6 cut(s) 311, 586, 903, 1116, 1588, 2235
CciI TCATGA 2 cut(s) 1300, 1801
CfoI GCGC 6 cut(s) 13, 47, 309, 1309, 1586, 2300
Cfr13I GGNCC 4 cut(s) 121, 144, 436, 1981
CseI GACGC 1 cut(s) 19
Csp6I GTAC 6 cut(s) 506, 691, 1128, 1444, 1781, 1854
CviQI GTAC 6 cut(s) 506, 691, 1128, 1444, 1781, 1854
DdeI CTNAG 5 cut(s) 906, 1069, 1366, 1458, 1580
DinI GGCGCC 2 cut(s) 12, 46
DpnI GATC 6 cut(s) 17, 660, 1165, 1271, 1358, 1488
DpnII GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
DraIII CACNNNGTG 2 cut(s) 727, 2032
EaeI YGGCCR 2 cut(s) 628, 1719
Eam1104I CTCTTC 6 cut(s) 515, 535, 878, 1492, 1929, 2256
EarI CTCTTC 6 cut(s) 515, 535, 878, 1492, 1929, 2256
EciI GGCGGA 1 cut(s) 1568
Ecl136II GAGCTC 1 cut(s) 2190
Eco130I CCWWGG 3 cut(s) 391, 1294, 1390
Eco24I GRGCYC 1 cut(s) 2192
Eco31I GGTCTC 2 cut(s) 744, 2109
Eco47I GGWCC 2 cut(s) 121, 436
Eco47III AGCGCT 2 cut(s) 1308, 1585
Eco53kI GAGCTC 1 cut(s) 2190
EcoICRI GAGCTC 1 cut(s) 2190
EcoRI GAATTC 2 cut(s) 766, 1805
EcoRII CCWGG 2 cut(s) 1765, 2262
EcoT14I CCWWGG 3 cut(s) 391, 1294, 1390
EcoT22I ATGCAT 4 cut(s) 679, 930, 1290, 1481
EcoT38I GRGCYC 1 cut(s) 2192
EgeI GGCGCC 2 cut(s) 12, 46
EheI GGCGCC 2 cut(s) 12, 46
ErhI CCWWGG 3 cut(s) 391, 1294, 1390
FaqI GGGAC 2 cut(s) 857, 1268
Fnu4HI GCNGC 2 cut(s) 589, 1626
FokI GGATG 5 cut(s) 700, 1488, 1568, 1724, 2157
FriOI GRGCYC 1 cut(s) 2192
Fsp4HI GCNGC 2 cut(s) 589, 1626
FspAI RTGCGCAY 1 cut(s) 2299
FspBI CTAG 5 cut(s) 401, 1295, 1400, 1878, 2243
FspI TGCGCA 1 cut(s) 2299
GlaI GCGC 6 cut(s) 12, 46, 308, 1308, 1585, 2299
GluI GCNGC 2 cut(s) 589, 1626
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 1 cut(s) 2106
HaeII RGCGCY 4 cut(s) 14, 48, 1310, 1587
HaeIII GGCC 8 cut(s) 145, 399, 630, 1039, 1278, 1293, 1721, 1982
HapII CCGG 7 cut(s) 97, 113, 130, 161, 434, 836, 1049
HgaI GACGC 1 cut(s) 19
HhaI GCGC 6 cut(s) 13, 47, 309, 1309, 1586, 2300
Hin1I GRCGYC 3 cut(s) 11, 45, 953
Hin6I GCGC 6 cut(s) 11, 45, 307, 1307, 1584, 2298
HinP1I GCGC 6 cut(s) 11, 45, 307, 1307, 1584, 2298
HincII GTYRAC 1 cut(s) 1525
HindII GTYRAC 1 cut(s) 1525
HinfI GANTC 9 cut(s) 429, 1024, 1462, 1594, 1658, 1684, 1762, 1952, 2183
HpaII CCGG 7 cut(s) 97, 113, 130, 161, 434, 836, 1049
HphI GGTGA 3 cut(s) 223, 250, 974
Hpy166II GTNNAC 4 cut(s) 154, 219, 346, 1525
Hpy188I TCNGA 8 cut(s) 38, 83, 125, 214, 481, 697, 747, 990
Hpy188III TCNNGA 8 cut(s) 155, 174, 836, 1301, 1681, 1786, 1802, 2085
Hpy8I GTNNAC 4 cut(s) 154, 219, 346, 1525
Hpy99I CGWCG 3 cut(s) 35, 38, 152
HpyAV CCTTC 9 cut(s) 35, 135, 457, 985, 1094, 1403, 1564, 1813, 1886
HpyCH4III ACNGT 4 cut(s) 986, 1421, 1841, 2126
HpyCH4IV ACGT 3 cut(s) 598, 953, 1527
HpyF10VI GCNNNNNNNGC 3 cut(s) 1634, 2196, 2243
HpyF3I CTNAG 5 cut(s) 906, 1069, 1366, 1458, 1580
HpySE526I ACGT 3 cut(s) 598, 953, 1527
Hsp92I GRCGYC 3 cut(s) 11, 45, 953
HspAI GCGC 6 cut(s) 11, 45, 307, 1307, 1584, 2298
KasI GGCGCC 2 cut(s) 10, 44
Kpn2I TCCGGA 1 cut(s) 835
Kzo9I GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
LguI GCTCTTC 1 cut(s) 1492
LmnI GCTCC 5 cut(s) 105, 389, 570, 697, 1654
Lsp1109I GCAGC 2 cut(s) 600, 1612
LweI GCATC 5 cut(s) 937, 1033, 1466, 1546, 2255
MaeI CTAG 5 cut(s) 401, 1295, 1400, 1878, 2243
MaeII ACGT 3 cut(s) 598, 953, 1527
MaeIII GTNAC 5 cut(s) 256, 594, 943, 1385, 1667
MalI GATC 6 cut(s) 17, 660, 1165, 1271, 1358, 1488
MboI GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
MfeI CAATTG 1 cut(s) 1611
MhlI GDGCHC 3 cut(s) 221, 1509, 2192
MlsI TGGCCA 2 cut(s) 630, 1721
MluNI TGGCCA 2 cut(s) 630, 1721
Mly113I GGCGCC 2 cut(s) 11, 45
MlyI GAGTC 3 cut(s) 1033, 1652, 1946
MmeI TCCRAC 3 cut(s) 16, 148, 518
Mox20I TGGCCA 2 cut(s) 630, 1721
Mph1103I ATGCAT 4 cut(s) 679, 930, 1290, 1481
MroI TCCGGA 1 cut(s) 835
MscI TGGCCA 2 cut(s) 630, 1721
MseI TTAA 4 cut(s) 248, 825, 1833, 2042
MslI CAYNNNNRTG 5 cut(s) 393, 936, 1331, 1602, 2293
Msp20I TGGCCA 2 cut(s) 630, 1721
MspCI CTTAAG 1 cut(s) 2041
MspI CCGG 7 cut(s) 97, 113, 130, 161, 434, 836, 1049
MspR9I CCNGG 7 cut(s) 114, 131, 162, 434, 1049, 1767, 2264
MunI CAATTG 1 cut(s) 1611
Mva1269I GAATGC 4 cut(s) 679, 1032, 1213, 2235
MvaI CCWGG 2 cut(s) 1767, 2264
MwoI GCNNNNNNNGC 3 cut(s) 1634, 2196, 2243
NarI GGCGCC 2 cut(s) 11, 45
NciI CCSGG 5 cut(s) 114, 131, 162, 434, 1049
NcoI CCATGG 2 cut(s) 391, 1390
NdeII GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
NlaIV GGNNCC 6 cut(s) 12, 46, 122, 438, 834, 1650
NmuCI GTSAC 2 cut(s) 256, 594
NsbI TGCGCA 1 cut(s) 2299
NsiI ATGCAT 4 cut(s) 679, 930, 1290, 1481
NspI RCATGY 2 cut(s) 526, 1118
NspV TTCGAA 1 cut(s) 1756
OliI CACNNNNGTG 1 cut(s) 393
PaeI GCATGC 1 cut(s) 1118
PagI TCATGA 2 cut(s) 1300, 1801
PciSI GCTCTTC 1 cut(s) 1492
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 4 cut(s) 679, 1032, 1213, 2235
PfeI GAWTC 6 cut(s) 429, 1462, 1594, 1684, 1762, 2183
PfoI TCCNGGA 1 cut(s) 432
PkrI GCNGC 2 cut(s) 590, 1627
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 3 cut(s) 1032, 1652, 1946
PluTI GGCGCC 2 cut(s) 14, 48
PpsI GAGTC 3 cut(s) 1032, 1652, 1946
PsiI TTATAA 1 cut(s) 2094
Psp124BI GAGCTC 1 cut(s) 2192
Psp6I CCWGG 2 cut(s) 1765, 2262
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 2 cut(s) 1765, 2262
PspN4I GGNNCC 6 cut(s) 12, 46, 122, 438, 834, 1650
PspPI GGNCC 4 cut(s) 121, 144, 436, 1981
PvuI CGATCG 1 cut(s) 18
RsaI GTAC 6 cut(s) 507, 692, 1129, 1445, 1782, 1855
RsaNI GTAC 6 cut(s) 506, 691, 1128, 1444, 1781, 1854
RseI CAYNNNNRTG 5 cut(s) 393, 936, 1331, 1602, 2293
SacI GAGCTC 1 cut(s) 2192
SapI GCTCTTC 1 cut(s) 1492
SaqAI TTAA 4 cut(s) 248, 825, 1833, 2042
SatI GCNGC 2 cut(s) 589, 1626
Sau3AI GATC 6 cut(s) 15, 658, 1163, 1269, 1356, 1486
Sau96I GGNCC 4 cut(s) 121, 144, 436, 1981
ScaI AGTACT 1 cut(s) 1782
SchI GAGTC 3 cut(s) 1033, 1652, 1946
ScrFI CCNGG 7 cut(s) 114, 131, 162, 434, 1049, 1767, 2264
SduI GDGCHC 3 cut(s) 221, 1509, 2192
SfaNI GCATC 5 cut(s) 937, 1033, 1466, 1546, 2255
SfcI CTRYAG 1 cut(s) 1560
SfoI GGCGCC 2 cut(s) 12, 46
SfuI TTCGAA 1 cut(s) 1756
SinI GGWCC 2 cut(s) 121, 436
SmiMI CAYNNNNRTG 5 cut(s) 393, 936, 1331, 1602, 2293
SmlI CTYRAG 4 cut(s) 1631, 1660, 1914, 2041
SmoI CTYRAG 4 cut(s) 1631, 1660, 1914, 2041
SphI GCATGC 1 cut(s) 1118
SsiI CCGC 4 cut(s) 234, 503, 1520, 1553
SspDI GGCGCC 2 cut(s) 10, 44
SspMI CTAG 5 cut(s) 401, 1295, 1400, 1878, 2243
SstI GAGCTC 1 cut(s) 2192
StyD4I CCNGG 7 cut(s) 112, 129, 160, 432, 1047, 1765, 2262
StyI CCWWGG 3 cut(s) 391, 1294, 1390
TaaI ACNGT 4 cut(s) 986, 1421, 1841, 2126
TaiI ACGT 3 cut(s) 601, 956, 1530
TaqI TCGA 8 cut(s) 18, 427, 555, 1682, 1756, 2101, 2186, 2281
TaqII GACCGA 1 cut(s) 72
TatI WGTACW 2 cut(s) 690, 1780
TfiI GAWTC 6 cut(s) 429, 1462, 1594, 1684, 1762, 2183
Tru1I TTAA 4 cut(s) 248, 825, 1833, 2042
Tru9I TTAA 4 cut(s) 248, 825, 1833, 2042
TscAI CASTG 2 cut(s) 777, 1846
TseFI GTSAC 2 cut(s) 256, 594
TseI GCWGC 2 cut(s) 588, 1625
Tsp45I GTSAC 2 cut(s) 256, 594
TspGWI ACGGA 3 cut(s) 118, 651, 1461
TspRI CASTG 2 cut(s) 777, 1846
Vha464I CTTAAG 1 cut(s) 2041
VneI GTGCAC 1 cut(s) 217
VpaK11BI GGWCC 2 cut(s) 121, 436
XapI RAATTY 4 cut(s) 541, 766, 1805, 2256
XceI RCATGY 2 cut(s) 526, 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmaJI CCTAGG 1 cut(s) 1294
XspI CTAG 5 cut(s) 401, 1295, 1400, 1878, 2243
ZraI GACGTC 1 cut(s) 954
ZrmI AGTACT 1 cut(s) 1782
Zsp2I ATGCAT 4 cut(s) 679, 930, 1290, 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.