Rh5AG297500
ERF Family

BEST Arabidopsis thaliana protein match is alpha beta-Hydrolases superfamily protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017741
Physical Location & Seq
Forward (+)
41505833 .. 41512539
6707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG297500.1

Sequence Viewer

Length: 2691 bp
ATGTCCGAGAGTTCAAGTCCAACAGAAGCTGTACTCTTCTACGCTCGCATGCTCTCTTCTTTGGAAAATTTGAACGGGGTCGAGTTTTTGCTCCCTGCATTGCTCAAAGCGTGCAGCAAGTCACGTGCTTTAGAGGAGGGGAGGCAAGTTCATGGCCAGATATTGAAAACCCAGATGTTGTTCGATCCGTATGTTGCGAATGCATTGATTAGGATGTACTCGGAGCTGGGATGTATTGGATTGGCACACCGGGTGTTTGATAAAATAGCTCAGAGAGACCAAGTTTCTTGGAATTCACTGATTACTGGTTATTTGAGGGTCGGGGAGGTTGAGTTGGCTCGTGCGATTTTTAATGAGATTCCGGAGAGGGACTTGGTTTCGTGTAATGCAATGATTGATGGGTATGGGAAGAGTGGCAAGTGTGAGCTTGCTGAGGAGGTTTTTAGGACAATGCATCATAAAGATGTGGTTACTTGGACGTCAATGGTTTCAGGGTATGTATTCAATCACCGTCCGAAGGAAGCATTGGGTTTGTTTAGGGAAATGTTGAGTCAGGGCATTCGGCCTGATGCCCCGGCTCTTGTTAGTGTTCTCTCAGCCATTGCCGACTTGGGTTTCGTTGAGGAAGGCAAATGGGTGCATGCTTTTATTTGTACGAATAAGATTGGGTTGAATTCAGGGTTTATTGGATCAGCTCTTATAGATATGTATGCTAAATGTGGGCATATAGAGAATGCTTACCATGTCTTTGAAAACATAGCTCAATATAGGAACATTGGGGACTGGAATTGCATGATCTCTGGCCTTGCTATGCATGGCCTAGGTCATGAAGCGCTGGACATTTTCCTTGACATGAAAATGAAAGAAATTGAGCCTGATGAGATCACTTTCTTAGGACTTCTTACTGCTTGTAACCATGGAGGTCTAGTTGATGAAGGTAGAGCAGCGCACTTGGAGGAGGCGGTTGACGTTATACAGAATATGCCCATAGAGGCGGATGCTATAGTTTGGAAGGCAATTCTCAGCGCTTGCACGATTCATGGTAATGTTGCAACTGGGGAAAGTGCTGCCCTCAAGGCAATAGATTTGGCTCCAGATGACTCAAGTTGTTACATTCTCCTCTCGAATCTTTATGCCAAAATAGGGAGATGGGATGATGTGGGCAATGTTAGATTGATGATGAAACAGAGGGGAGTTCGAAAGATTCCAGGGTCAAGTTCAGTACTTGTGAATGGAAAGGTTCATGAATTCCTTGTGGGGAAGGAAATGAATGTTAAATACAGTGTTGAGCTGCATCATCCTGTGGAAGAATCGAGCTCAATGGGCAGGTTTCAGTCTCAATCTTTGGCATTAGGAATGCAGACTACATTAGCATCTCTTCAAATTCCCCTGGAAAGGAGGGTGGCTCAATTATCACTAATATGGGCATTTAGAGGAATCCCAGATTGGTTTTTCGCGAGTACTGAGAGAGGAGTCGACCAAGCTAGTCTCACTTCGTCCCAATTTTTCAGCAGGTGGGCTTTTGATTTCTTCTGTCTTTTTGCGATTTTGTTTATCTTTTTCTTTCCAGTGTGCTCAAAATCACTTGCCAGGTGGTTTGCGCTGCCTCAGCCGGGGATCACAGTCACCAGAGGAACAAGAGAGCCAGACCCTTTTCAACTTTCAGCAATGATTGCCCCAGTAGTACTAGCTTTGACAGTGGGTTTTCTTGGGTGGGCATATCAGGCACTAAAGCCTCCCCCTCCTAAAATATGCGGTTCACCTAGTGGTCCTCCTGTCACTTCACCTAGAGTGAAACTCAGTGATGGTAGATATTTGGCCTACAGGGAGTTTGGAGTGCCTAAAGAAGAGGCAAAACACAAGATCATCATCATTCATGGCTTTAGCAGTTCCAAAGATCTGGCTTTACCTGTTTCTCAAGAACTCGTTGACGAGTTAAGGGTATATTTTCTCTTCTTTGACAGAGCTGGTTATGGTGAAAGTGATCCGTATCCCTCACGCTCGGTTAAGAGTGAAGCATATGATATTCAAGAACTAGCCGACACGTTGCAGATTGGGTCCAAATTCTATGTAATTGGAATCTCAATGGGGGCTTACCCTGTTTGGAGTTGCTTGAAATACATACCGCACAGATTGTTAGGAGCTTCCCTGGTAGTTCCTTTTGTGAACTACTGGTGGCCTCGTATTCCTGCTAATCTATTGAAAGAGTCCTTTCAGAGGCTGCAGGTATCAGACCAACGGACGTTCCAAGTTGCACATTACACCCCATGGTTGCTTCATTGGTGGATGACCCAGAAATGGTTCACTGCATTAAGTATCATGGCTGGAAATATGGAGATCTTCTGCCCCAAAGATTTAGAAACATTGAAGAAACTTTCCGAATCCCCAAGTGTTGGTCAGGAAAAGATACAGCAGCAAGGAGAACACGAATCTCTCTACCGAGACCTACTGGCTGGCTATGGAAAATGGGAATTCGACCCCATAGACTTAACGAATCCATTTCCTAACAATGAGGGTTCAGTTCACATTTGGCAAGGCTATGCAGACAGGATCATTCCATATAAAGTAAACCGTTACATTGCAGAGAAGCTTCCCTGGATTCATTATCACGAAGTTCCTGATTATGGGCATTTCCTTATTTTTGAGAGTGATCTATGTGAGGCTGTTTTGAAGGCACTCTTGCGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

896

Amino Acids

100.68

Weight (kDa)

6.03

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 126 - 155 8.3e-07 PPR repeat
PPR_2 PF13041 154 - 191 1.3e-08 PPR repeat family
PPR PF01535 157 - 187 2.2e-07 PPR repeat
PPR_2 PF13041 262 - 304 2.5e-09 PPR repeat family
E_motif PF20431 347 - 408 2.2e-16 E motif
Hydrolase_4 PF12146 617 - 883 7.5e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 622 - 883 1.1e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 622 - 884 5.6e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017741)

Species Orthologous Gene IDs
malus_domestica MD11G1163700.v1.1
pyrus_communis pycom11g13520
rosa_chinensis RchiOBHm_Chr5g0044291
rosa_laevigata RLG00000034263
rosa_multiflora Rmu_sc0015666.1_g000004
rosa_roxburghii Rroxscaffold_1G00036670
rosa_rugosa Rorug05G0212900
rosa_samantha Rh5AG297500 Rh5BG304800 Rh5CG331700 Rh5DG314000
rosa_wichuraiana Rw5G027530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1503
AatII GACGTC 1 cut(s) 482
Acc36I ACCTGC 3 cut(s) 1317, 1503, 2215
AccB7I CCANNNNNTGG 1 cut(s) 2393
AccI GTMKAC 1 cut(s) 1476
AccII CGCG 1 cut(s) 1457
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 4 cut(s) 962, 995, 1755, 2126
AclWI GGATC 5 cut(s) 179, 697, 1625, 1979, 2558
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 7 cut(s) 67, 292, 673, 1247, 1383, 2063, 2471
AcvI CACGTG 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 479
AdeI CACNNNGTG 2 cut(s) 253, 1766
AfaI GTAC 6 cut(s) 33, 218, 655, 1224, 1462, 1686
AfeI AGCGCT 2 cut(s) 834, 1027
AfiI CCNNNNNNNGG 8 cut(s) 517, 1143, 1395, 1613, 2217, 2298, 2393, 2624
AflIII ACRYGT 1 cut(s) 2043
AjnI CCWGG 5 cut(s) 1207, 1389, 1589, 2148, 2594
AjuI GAANNNNNNNTTGG 4 cut(s) 509, 541, 1429, 1461
Alw21I GWGCWC 2 cut(s) 1319, 1577
Alw26I GTCTC 4 cut(s) 270, 1341, 1493, 2436
AlwI GGATC 5 cut(s) 179, 697, 1625, 1979, 2558
AlwNI CAGNNNCTG 2 cut(s) 29, 2221
Aor13HI TCCGGA 1 cut(s) 361
Aor51HI AGCGCT 2 cut(s) 834, 1027
AoxI GGCC 6 cut(s) 154, 563, 802, 817, 1818, 2177
ApeKI GCWGC 7 cut(s) 114, 944, 1067, 1291, 1603, 2221, 2413
ApoI RAATTY 7 cut(s) 67, 292, 673, 1247, 1383, 2063, 2471
ArsI GACNNNNNNTTYG 2 cut(s) 599, 631
Asp700I GAANNNNTTC 1 cut(s) 2300
AspA2I CCTAGG 1 cut(s) 820
AspLEI GCGC 4 cut(s) 835, 949, 1028, 1603
AspS9I GGNCC 2 cut(s) 1769, 2058
AsuC2I CCSGG 3 cut(s) 251, 575, 1614
AsuHPI GGTGA 5 cut(s) 500, 1618, 1752, 1776, 1988
AsuII TTCGAA 1 cut(s) 1198
AvaII GGWCC 2 cut(s) 1769, 2058
AvrII CCTAGG 1 cut(s) 820
BalI TGGCCA 1 cut(s) 156
BanII GRGCYC 1 cut(s) 1319
BauI CACGAG 1 cut(s) 339
BbrPI CACGTG 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 1319, 1577
BbvCI CCTCAGC 2 cut(s) 432, 1608
BbvI GCAGC 7 cut(s) 126, 956, 1054, 1278, 1590, 2208, 2425
BccI CCATC 3 cut(s) 392, 1143, 1799
BciT130I CCWGG 5 cut(s) 1209, 1391, 1591, 2150, 2596
BciVI GTATCC 1 cut(s) 2001
BcnI CCSGG 3 cut(s) 251, 575, 1614
BcoDI GTCTC 4 cut(s) 270, 1341, 1493, 2436
BfaI CTAG 7 cut(s) 821, 926, 1485, 1688, 1764, 1788, 2036
BfmI CTRYAG 3 cut(s) 1002, 1822, 2222
BfoI RGCGCY 2 cut(s) 836, 1029
BfuAI ACCTGC 3 cut(s) 1317, 1503, 2215
BfuI GTATCC 1 cut(s) 2001
BglI GCCNNNNNGGC 1 cut(s) 1076
BglII AGATCT 2 cut(s) 1897, 2337
BisI GCNGC 7 cut(s) 115, 945, 1068, 1292, 1604, 2222, 2414
BlnI CCTAGG 1 cut(s) 820
BlsI GCNGC 7 cut(s) 116, 946, 1069, 1293, 1605, 2223, 2415
BmcAI AGTACT 3 cut(s) 1224, 1462, 1686
Bme1390I CCNGG 8 cut(s) 251, 575, 1209, 1391, 1591, 1614, 2150, 2596
Bme18I GGWCC 2 cut(s) 1769, 2058
BmgT120I GGNCC 2 cut(s) 1769, 2058
BmiI GGNNCC 2 cut(s) 1092, 2059
BmrFI CCNGG 8 cut(s) 251, 575, 1209, 1391, 1591, 1614, 2150, 2596
BmrI ACTGGG 2 cut(s) 1065, 1673
BmsI GCATC 5 cut(s) 463, 559, 988, 1303, 1382
BmuI ACTGGG 2 cut(s) 1065, 1673
BplI GAGNNNNNCTC 4 cut(s) 1390, 1422, 1782, 1814
BpmI CTGGAG 1 cut(s) 1077
Bpu10I CCTNAGC 2 cut(s) 432, 1608
Bpu14I TTCGAA 1 cut(s) 1198
BpuEI CTTGAG 3 cut(s) 1058, 1087, 1902
BpuMI CCSGG 3 cut(s) 251, 575, 1614
BsaAI YACGTR 1 cut(s) 125
BsaBI GATNNNNATC 2 cut(s) 1868, 1989
BsaHI GRCGYC 1 cut(s) 479
BsaI GGTCTC 2 cut(s) 270, 2436
BsaJI CCNNGG 9 cut(s) 573, 820, 916, 1208, 1389, 1613, 2148, 2267, 2594
BsaWI WCCGGW 1 cut(s) 361
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 912, 942
Bsc4I CCNNNNNNNGG 8 cut(s) 517, 1143, 1395, 1613, 2217, 2298, 2393, 2624
Bse1I ACTGG 7 cut(s) 310, 788, 1060, 1568, 1679, 2177, 2454
Bse3DI GCAATG 6 cut(s) 98, 396, 600, 1171, 1674, 2577
Bse8I GATNNNNATC 2 cut(s) 1868, 1989
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 5 cut(s) 1209, 1391, 1591, 2150, 2596
BseDI CCNNGG 9 cut(s) 573, 820, 916, 1208, 1389, 1613, 2148, 2267, 2594
BseGI GGATG 6 cut(s) 219, 236, 1003, 1159, 1297, 2292
BseJI GATNNNNATC 2 cut(s) 1868, 1989
BseLI CCNNNNNNNGG 8 cut(s) 517, 1143, 1395, 1613, 2217, 2298, 2393, 2624
BseMI GCAATG 6 cut(s) 98, 396, 600, 1171, 1674, 2577
BseMII CTCAG 7 cut(s) 284, 423, 609, 1036, 1455, 1622, 1813
BseNI ACTGG 7 cut(s) 310, 788, 1060, 1568, 1679, 2177, 2454
BseRI GAGGAG 5 cut(s) 149, 449, 971, 1109, 1485
BseXI GCAGC 7 cut(s) 126, 956, 1054, 1278, 1590, 2208, 2425
BseYI CCCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 1457
BshFI GGCC 6 cut(s) 156, 565, 804, 819, 1820, 2179
BsiHKAI GWGCWC 2 cut(s) 1319, 1577
BsiSI CCGG 4 cut(s) 250, 362, 575, 1613
BslFI GGGAC 3 cut(s) 383, 794, 1483
BslI CCNNNNNNNGG 8 cut(s) 517, 1143, 1395, 1613, 2217, 2298, 2393, 2624
BsmAI GTCTC 4 cut(s) 270, 1341, 1493, 2436
BsmFI GGGAC 3 cut(s) 383, 794, 1483
BsmI GAATGC 4 cut(s) 205, 558, 739, 1362
BsnI GGCC 6 cut(s) 156, 565, 804, 819, 1820, 2179
Bso31I GGTCTC 2 cut(s) 270, 2436
Bsp119I TTCGAA 1 cut(s) 1198
Bsp1286I GDGCHC 2 cut(s) 1319, 1577
Bsp13I TCCGGA 1 cut(s) 361
Bsp19I CCATGG 2 cut(s) 916, 2267
Bsp68I TCGCGA 1 cut(s) 1457
BspACI CCGC 4 cut(s) 962, 995, 1755, 2126
BspANI GGCC 6 cut(s) 156, 565, 804, 819, 1820, 2179
BspCNI CTCAG 7 cut(s) 283, 424, 608, 1035, 1456, 1621, 1812
BspEI TCCGGA 1 cut(s) 361
BspFNI CGCG 1 cut(s) 1457
BspHI TCATGA 2 cut(s) 826, 1243
BspLI GGNNCC 2 cut(s) 1092, 2059
BspMAI CTGCAG 1 cut(s) 2226
BspMI ACCTGC 3 cut(s) 1317, 1503, 2215
BspPI GGATC 5 cut(s) 179, 697, 1625, 1979, 2558
BspT104I TTCGAA 1 cut(s) 1198
BspTNI GGTCTC 2 cut(s) 270, 2436
BsrDI GCAATG 6 cut(s) 98, 396, 600, 1171, 1674, 2577
BsrI ACTGG 7 cut(s) 310, 788, 1060, 1568, 1679, 2177, 2454
BssECI CCNNGG 9 cut(s) 573, 820, 916, 1208, 1389, 1613, 2148, 2267, 2594
BssNI GRCGYC 1 cut(s) 479
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 3 cut(s) 820, 916, 2267
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 5 cut(s) 1209, 1391, 1591, 2150, 2596
Bst4CI ACNGT 5 cut(s) 512, 1283, 1624, 1699, 2573
Bst6I CTCTTC 6 cut(s) 41, 61, 404, 1383, 1842, 1958
BstACI GRCGYC 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 1673
BstBAI YACGTR 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 1198
BstC8I GCNNGC 7 cut(s) 46, 50, 112, 429, 642, 1030, 2455
BstDEI CTNAG 8 cut(s) 270, 432, 595, 892, 1022, 1464, 1608, 1799
BstDSI CCRYGG 2 cut(s) 916, 2267
BstENI CCTNNNNNAGG 1 cut(s) 2215
BstF5I GGATG 6 cut(s) 219, 236, 1003, 1159, 1297, 2292
BstFNI CGCG 1 cut(s) 1457
BstH2I RGCGCY 2 cut(s) 836, 1029
BstHHI GCGC 4 cut(s) 835, 949, 1028, 1603
BstMAI GTCTC 4 cut(s) 270, 1341, 1493, 2436
BstMWI GCNNNNNNNGC 5 cut(s) 1076, 1323, 1609, 1673, 1724
BstNI CCWGG 5 cut(s) 1209, 1391, 1591, 2150, 2596
BstNSI RCATGY 2 cut(s) 52, 644
BstSCI CCNGG 8 cut(s) 249, 573, 1207, 1389, 1589, 1612, 2148, 2594
BstSFI CTRYAG 3 cut(s) 1002, 1822, 2222
BstUI CGCG 1 cut(s) 1457
BstV1I GCAGC 7 cut(s) 126, 956, 1054, 1278, 1590, 2208, 2425
BstX2I RGATCY 2 cut(s) 1897, 2337
BstXI CCANNNNNNTGG 1 cut(s) 1900
BstYI RGATCY 2 cut(s) 1897, 2337
BsuI GTATCC 1 cut(s) 2001
BsuRI GGCC 6 cut(s) 156, 565, 804, 819, 1820, 2179
BtgI CCRYGG 2 cut(s) 916, 2267
BtsCI GGATG 6 cut(s) 219, 236, 1003, 1159, 1297, 2292
BtsI GCAGTG 1 cut(s) 2304
BtsIMutI CAGTG 6 cut(s) 296, 1288, 1575, 1704, 1807, 2304
BtuMI TCGCGA 1 cut(s) 1457
BveI ACCTGC 3 cut(s) 1317, 1503, 2215
Cac8I GCNNGC 7 cut(s) 46, 50, 112, 429, 642, 1030, 2455
CaiI CAGNNNCTG 2 cut(s) 29, 2221
CciI TCATGA 2 cut(s) 826, 1243
CfoI GCGC 4 cut(s) 835, 949, 1028, 1603
Cfr13I GGNCC 2 cut(s) 1769, 2058
Csp6I GTAC 6 cut(s) 32, 217, 654, 1223, 1461, 1685
CviQI GTAC 6 cut(s) 32, 217, 654, 1223, 1461, 1685
DdeI CTNAG 8 cut(s) 270, 432, 595, 892, 1022, 1464, 1608, 1799
DraIII CACNNNGTG 2 cut(s) 253, 1766
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 6 cut(s) 41, 61, 404, 1383, 1842, 1958
EarI CTCTTC 6 cut(s) 41, 61, 404, 1383, 1842, 1958
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 1317
Eco130I CCWWGG 3 cut(s) 820, 916, 2267
Eco24I GRGCYC 1 cut(s) 1319
Eco31I GGTCTC 2 cut(s) 270, 2436
Eco47I GGWCC 2 cut(s) 1769, 2058
Eco47III AGCGCT 2 cut(s) 834, 1027
Eco53kI GAGCTC 1 cut(s) 1317
Eco72I CACGTG 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 1317
EcoNI CCTNNNNNAGG 1 cut(s) 2215
EcoRI GAATTC 4 cut(s) 292, 673, 1247, 2471
EcoRII CCWGG 5 cut(s) 1207, 1389, 1589, 2148, 2594
EcoT14I CCWWGG 3 cut(s) 820, 916, 2267
EcoT22I ATGCAT 3 cut(s) 205, 456, 816
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 3 cut(s) 820, 916, 2267
FalI AAGNNNNNCTT 1 cut(s) 2663
FaqI GGGAC 3 cut(s) 383, 794, 1483
FauNDI CATATG 1 cut(s) 2020
FblI GTMKAC 1 cut(s) 1476
Fnu4HI GCNGC 7 cut(s) 115, 945, 1068, 1292, 1604, 2222, 2414
FokI GGATG 6 cut(s) 226, 243, 1010, 1166, 1284, 2299
FriOI GRGCYC 1 cut(s) 1319
Fsp4HI GCNGC 7 cut(s) 115, 945, 1068, 1292, 1604, 2222, 2414
FspBI CTAG 7 cut(s) 821, 926, 1485, 1688, 1764, 1788, 2036
GlaI GCGC 4 cut(s) 834, 948, 1027, 1602
GluI GCNGC 7 cut(s) 115, 945, 1068, 1292, 1604, 2222, 2414
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 1077
HaeII RGCGCY 2 cut(s) 836, 1029
HaeIII GGCC 6 cut(s) 156, 565, 804, 819, 1820, 2179
HapII CCGG 4 cut(s) 250, 362, 575, 1613
HhaI GCGC 4 cut(s) 835, 949, 1028, 1603
Hin1I GRCGYC 1 cut(s) 479
Hin6I GCGC 4 cut(s) 833, 947, 1026, 1601
HinP1I GCGC 4 cut(s) 833, 947, 1026, 1601
HincII GTYRAC 3 cut(s) 967, 1477, 1930
HindII GTYRAC 3 cut(s) 967, 1477, 1930
HindIII AAGCTT 1 cut(s) 2588
HpaII CCGG 4 cut(s) 250, 362, 575, 1613
HphI GGTGA 5 cut(s) 500, 1618, 1752, 1776, 1988
Hpy166II GTNNAC 8 cut(s) 967, 1477, 1760, 1930, 2167, 2304, 2524, 2569
Hpy188I TCNGA 7 cut(s) 7, 223, 273, 516, 2217, 2233, 2380
Hpy8I GTNNAC 8 cut(s) 967, 1477, 1760, 1930, 2167, 2304, 2524, 2569
HpyAV CCTTC 6 cut(s) 511, 620, 929, 1006, 1255, 2665
HpyCH4III ACNGT 5 cut(s) 512, 1283, 1624, 1699, 2573
HpyCH4IV ACGT 5 cut(s) 124, 479, 969, 2045, 2243
HpyF10VI GCNNNNNNNGC 5 cut(s) 1076, 1323, 1609, 1673, 1724
HpyF3I CTNAG 8 cut(s) 270, 432, 595, 892, 1022, 1464, 1608, 1799
HpySE526I ACGT 5 cut(s) 124, 479, 969, 2045, 2243
Hsp92I GRCGYC 1 cut(s) 479
HspAI GCGC 4 cut(s) 833, 947, 1026, 1601
Kpn2I TCCGGA 1 cut(s) 361
LmnI GCTCC 4 cut(s) 96, 223, 1096, 2141
Lsp1109I GCAGC 7 cut(s) 126, 956, 1054, 1278, 1590, 2208, 2425
LweI GCATC 5 cut(s) 463, 559, 988, 1303, 1382
MaeI CTAG 7 cut(s) 821, 926, 1485, 1688, 1764, 1788, 2036
MaeII ACGT 5 cut(s) 124, 479, 969, 2045, 2243
MaeIII GTNAC 7 cut(s) 120, 469, 911, 1109, 1624, 1777, 2573
MflI RGATCY 2 cut(s) 1897, 2337
MhlI GDGCHC 2 cut(s) 1319, 1577
MlsI TGGCCA 1 cut(s) 156
MluNI TGGCCA 1 cut(s) 156
MlyI GAGTC 4 cut(s) 559, 1094, 1482, 2216
MmeI TCCRAC 1 cut(s) 44
Mox20I TGGCCA 1 cut(s) 156
Mph1103I ATGCAT 3 cut(s) 205, 456, 816
MroI TCCGGA 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 2300
MscI TGGCCA 1 cut(s) 156
MseI TTAA 6 cut(s) 351, 1275, 1937, 2007, 2312, 2489
MslI CAYNNNNRTG 4 cut(s) 462, 857, 1044, 1420
Msp20I TGGCCA 1 cut(s) 156
MspI CCGG 4 cut(s) 250, 362, 575, 1613
MspR9I CCNGG 8 cut(s) 251, 575, 1209, 1391, 1591, 1614, 2150, 2596
Mva1269I GAATGC 4 cut(s) 205, 558, 739, 1362
MvaI CCWGG 5 cut(s) 1209, 1391, 1591, 2150, 2596
MvnI CGCG 1 cut(s) 1457
MwoI GCNNNNNNNGC 5 cut(s) 1076, 1323, 1609, 1673, 1724
NciI CCSGG 3 cut(s) 251, 575, 1614
NcoI CCATGG 2 cut(s) 916, 2267
NdeI CATATG 1 cut(s) 2020
NlaIV GGNNCC 2 cut(s) 1092, 2059
NmuCI GTSAC 3 cut(s) 120, 1624, 1777
NruI TCGCGA 1 cut(s) 1457
NsiI ATGCAT 3 cut(s) 205, 456, 816
NspI RCATGY 2 cut(s) 52, 644
NspV TTCGAA 1 cut(s) 1198
PaeI GCATGC 2 cut(s) 52, 644
PagI TCATGA 2 cut(s) 826, 1243
PaqCI CACCTGC 1 cut(s) 1503
PctI GAATGC 4 cut(s) 205, 558, 739, 1362
PdmI GAANNNNTTC 1 cut(s) 2300
PflMI CCANNNNNTGG 1 cut(s) 2393
PkrI GCNGC 7 cut(s) 116, 946, 1069, 1293, 1605, 2223, 2415
PleI GAGTC 4 cut(s) 558, 1094, 1481, 2215
PmaCI CACGTG 1 cut(s) 125
PmlI CACGTG 1 cut(s) 125
PpsI GAGTC 4 cut(s) 558, 1094, 1481, 2215
Ppu21I YACGTR 1 cut(s) 125
Psp124BI GAGCTC 1 cut(s) 1319
Psp6I CCWGG 5 cut(s) 1207, 1389, 1589, 2148, 2594
PspCI CACGTG 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 5 cut(s) 1207, 1389, 1589, 2148, 2594
PspN4I GGNNCC 2 cut(s) 1092, 2059
PspPI GGNCC 2 cut(s) 1769, 2058
PstI CTGCAG 1 cut(s) 2226
PstNI CAGNNNCTG 2 cut(s) 29, 2221
PsuI RGATCY 2 cut(s) 1897, 2337
RruI TCGCGA 1 cut(s) 1457
RsaI GTAC 6 cut(s) 33, 218, 655, 1224, 1462, 1686
RsaNI GTAC 6 cut(s) 32, 217, 654, 1223, 1461, 1685
RseI CAYNNNNRTG 4 cut(s) 462, 857, 1044, 1420
SacI GAGCTC 1 cut(s) 1319
SalI GTCGAC 1 cut(s) 1475
SaqAI TTAA 6 cut(s) 351, 1275, 1937, 2007, 2312, 2489
SatI GCNGC 7 cut(s) 115, 945, 1068, 1292, 1604, 2222, 2414
Sau96I GGNCC 2 cut(s) 1769, 2058
ScaI AGTACT 3 cut(s) 1224, 1462, 1686
SchI GAGTC 4 cut(s) 559, 1094, 1482, 2216
ScrFI CCNGG 8 cut(s) 251, 575, 1209, 1391, 1591, 1614, 2150, 2596
SduI GDGCHC 2 cut(s) 1319, 1577
SfaNI GCATC 5 cut(s) 463, 559, 988, 1303, 1382
SfcI CTRYAG 3 cut(s) 1002, 1822, 2222
SfuI TTCGAA 1 cut(s) 1198
SinI GGWCC 2 cut(s) 1769, 2058
SmiMI CAYNNNNRTG 4 cut(s) 462, 857, 1044, 1420
SmlI CTYRAG 3 cut(s) 1073, 1102, 1917
SmoI CTYRAG 3 cut(s) 1073, 1102, 1917
SphI GCATGC 2 cut(s) 52, 644
SsiI CCGC 4 cut(s) 962, 995, 1755, 2126
SspMI CTAG 7 cut(s) 821, 926, 1485, 1688, 1764, 1788, 2036
SstI GAGCTC 1 cut(s) 1319
StyD4I CCNGG 8 cut(s) 249, 573, 1207, 1389, 1589, 1612, 2148, 2594
StyI CCWWGG 3 cut(s) 820, 916, 2267
TaaI ACNGT 5 cut(s) 512, 1283, 1624, 1699, 2573
TaiI ACGT 5 cut(s) 127, 482, 972, 2048, 2246
TaqI TCGA 7 cut(s) 81, 183, 1124, 1198, 1313, 1476, 2475
TatI WGTACW 5 cut(s) 31, 216, 1222, 1460, 1684
Tru1I TTAA 6 cut(s) 351, 1275, 1937, 2007, 2312, 2489
Tru9I TTAA 6 cut(s) 351, 1275, 1937, 2007, 2312, 2489
TscAI CASTG 6 cut(s) 303, 1288, 1575, 1704, 1807, 2311
TseFI GTSAC 3 cut(s) 120, 1624, 1777
TseI GCWGC 7 cut(s) 114, 944, 1067, 1291, 1603, 2221, 2413
Tsp45I GTSAC 3 cut(s) 120, 1624, 1777
TspGWI ACGGA 3 cut(s) 177, 1977, 2254
TspRI CASTG 6 cut(s) 303, 1288, 1575, 1704, 1807, 2311
Van91I CCANNNNNTGG 1 cut(s) 2393
VpaK11BI GGWCC 2 cut(s) 1769, 2058
XagI CCTNNNNNAGG 1 cut(s) 2215
XapI RAATTY 7 cut(s) 67, 292, 673, 1247, 1383, 2063, 2471
XceI RCATGY 2 cut(s) 52, 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmaJI CCTAGG 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 1476
XmnI GAANNNNTTC 1 cut(s) 2300
XspI CTAG 7 cut(s) 821, 926, 1485, 1688, 1764, 1788, 2036
ZraI GACGTC 1 cut(s) 480
ZrmI AGTACT 3 cut(s) 1224, 1462, 1686
Zsp2I ATGCAT 3 cut(s) 205, 456, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.