RLG00000035228

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0020046
Physical Location & Seq
Reverse (-)
63220743 .. 63224335
3593 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035228

Sequence Viewer

Length: 696 bp
ATGTTGGGTCTACCGTCAGATCTTCTCCCCAACTGGGTTTTGCTAGAAACTGAACTAGAAGAGCTCGAATTGGAGCTGGAAGTTGTGGCGCCACCTCTATTGTTGTTAACACCATTCACTGAGTCATGGAAGCAATCTGTGAGAAATAGCAATGCAGAAGGAAATGATGGGTTAAGTCTTAGTACAAGTGGTTCTTTTGAATCCCAGAAGGCAAGTGATGAGGATGCTTGTTTGGTTACCACTTCTAGTTCCATTAATAAGCAGGAAGATGAAAATGTTGAGGAGATGGGTTTTTGGTTCAATTCAAGCTCAAGAGATGCAATGCAATGTGCTGAGCCAAAGAACTGCAACGTCTCGCTCCCTGTCGCCCTTTCAAACCTTCCACTCTTCCTCCACTTCTCTGGCAATCGAGGTAAGCTCAATCTCTTGCGCCCATACAGAAAATGTGATGTTGAGTTGGAAAGAAGGGGAGGAAGAAGAAAGTGGGGGAGAGTCGAAGGCGTGGTAGAAGAGGTTGAGTTAGAGAGAGCAGCTGCCGAGGAAGGAGCGAGACTAGGTCAAGAAGAAGAGGGCGAAGTCGGAGTGGTAAAGACATGTATTGAGGTTGGGGGTGGAGGCATCGACGGCGGTGGTGGTGGTTATGAGCAAAAGGGCTTGGGATTTAAGAGGCAAAATCAACAAAGTGAAGTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

232

Amino Acids

25.32

Weight (kDa)

4.6

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 10
AciI CCGC 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 4 cut(s) 200, 301, 306, 375
AluBI AGCT 5 cut(s) 64, 76, 309, 418, 533
AluI AGCT 5 cut(s) 64, 76, 309, 418, 533
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 2 cut(s) 358, 544
ApeKI GCWGC 2 cut(s) 530, 533
AseI ATTAAT 1 cut(s) 255
AspLEI GCGC 2 cut(s) 91, 432
BanI GGYRCC 1 cut(s) 88
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 520, 542
BccI CCATC 2 cut(s) 161, 280
BceAI ACGGC 1 cut(s) 640
BcoDI GTCTC 2 cut(s) 358, 544
BfaI CTAG 4 cut(s) 44, 56, 246, 554
BfoI RGCGCY 1 cut(s) 92
BglII AGATCT 1 cut(s) 19
BisI GCNGC 2 cut(s) 531, 534
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 2 cut(s) 532, 535
BmiI GGNNCC 1 cut(s) 90
BmrI ACTGGG 1 cut(s) 43
BmsI GCATC 3 cut(s) 214, 307, 627
BmuI ACTGGG 1 cut(s) 43
BplI GAGNNNNNCTC 2 cut(s) 402, 434
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 537
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 38
Bse3DI GCAATG 3 cut(s) 157, 327, 332
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 3 cut(s) 157, 327, 332
BseMII CTCAG 2 cut(s) 111, 324
BseNI ACTGG 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 296
BseXI GCAGC 2 cut(s) 520, 542
BshNI GGYRCC 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 358, 544
BsmBI CGTCTC 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 19
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 1 cut(s) 627
BspCNI CTCAG 2 cut(s) 112, 325
BspLI GGNNCC 1 cut(s) 90
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 88
BsrDI GCAATG 3 cut(s) 157, 327, 332
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 1 cut(s) 19
BssNI GRCGYC 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 15
Bst6I CTCTTC 4 cut(s) 54, 392, 504, 561
BstACI GRCGYC 1 cut(s) 89
BstDEI CTNAG 3 cut(s) 120, 179, 333
BstEII GGTNACC 1 cut(s) 235
BstF5I GGATG 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 91, 432
BstKTI GATC 1 cut(s) 22
BstMAI GTCTC 2 cut(s) 358, 544
BstMBI GATC 1 cut(s) 19
BstMWI GCNNNNNNNGC 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 597
BstPI GGTNACC 1 cut(s) 235
BstV1I GCAGC 2 cut(s) 520, 542
BstX2I RGATCY 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 1 cut(s) 19
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 117
CfoI GCGC 2 cut(s) 91, 432
Csp6I GTAC 1 cut(s) 183
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviAII CATG 2 cut(s) 126, 594
CviJI RGCY 7 cut(s) 64, 76, 309, 337, 418, 533, 654
CviKI_1 RGCY 7 cut(s) 64, 76, 309, 337, 418, 533, 654
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 3 cut(s) 120, 179, 333
DinI GGCGCC 1 cut(s) 90
DpnI GATC 1 cut(s) 21
DpnII GATC 1 cut(s) 19
Eam1104I CTCTTC 4 cut(s) 54, 392, 504, 561
EarI CTCTTC 4 cut(s) 54, 392, 504, 561
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
Eco91I GGTNACC 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 64
EcoO65I GGTNACC 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 2 cut(s) 129, 597
FaiI YATR 4 cut(s) 127, 436, 595, 642
FalI AAGNNNNNCTT 2 cut(s) 178, 210
FatI CATG 2 cut(s) 125, 593
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 2 cut(s) 531, 534
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 531, 534
FspBI CTAG 4 cut(s) 44, 56, 246, 554
GlaI GCGC 2 cut(s) 90, 431
GluI GCNGC 2 cut(s) 531, 534
HaeII RGCGCY 1 cut(s) 92
HhaI GCGC 2 cut(s) 91, 432
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 2 cut(s) 129, 597
Hin6I GCGC 2 cut(s) 89, 430
HinP1I GCGC 2 cut(s) 89, 430
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HinfI GANTC 3 cut(s) 122, 200, 492
HpaI GTTAAC 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 11, 108
Hpy188I TCNGA 2 cut(s) 19, 581
Hpy188III TCNNGA 2 cut(s) 312, 560
Hpy8I GTNNAC 2 cut(s) 11, 108
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 6 cut(s) 152, 202, 389, 459, 491, 536
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 155, 320, 325, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 624
HpyF3I CTNAG 3 cut(s) 120, 179, 333
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 2 cut(s) 129, 597
HspAI GCGC 2 cut(s) 89, 430
KasI GGCGCC 1 cut(s) 88
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 19
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 3 cut(s) 73, 363, 545
LpnPI CCDG 6 cut(s) 19, 62, 218, 248, 375, 387
Lsp1109I GCAGC 2 cut(s) 520, 542
LweI GCATC 3 cut(s) 214, 307, 627
MaeI CTAG 4 cut(s) 44, 56, 246, 554
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 21
MboI GATC 1 cut(s) 19
MboII GAAGA 9 cut(s) 14, 71, 278, 379, 486, 489, 521, 575, 578
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 2 cut(s) 68, 301
Mly113I GGCGCC 1 cut(s) 89
MlyI GAGTC 2 cut(s) 131, 501
MmeI TCCRAC 2 cut(s) 438, 559
MseI TTAA 4 cut(s) 107, 173, 255, 663
MspA1I CMGCKG 1 cut(s) 533
MwoI GCNNNNNNNGC 1 cut(s) 624
NarI GGCGCC 1 cut(s) 89
NdeII GATC 1 cut(s) 19
NlaIII CATG 2 cut(s) 129, 597
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 562
NspI RCATGY 1 cut(s) 597
PciI ACATGT 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 1 cut(s) 200
PflFI GACNNNGTC 1 cut(s) 555
PkrI GCNGC 2 cut(s) 532, 535
PleI GAGTC 2 cut(s) 130, 500
PluTI GGCGCC 1 cut(s) 92
PpsI GAGTC 2 cut(s) 130, 500
PscI ACATGT 1 cut(s) 593
PshBI ATTAAT 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 66
PspEI GGTNACC 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 90
PsrI GAACNNNNNNTAC 2 cut(s) 175, 207
PsuI RGATCY 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 555
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 4 cut(s) 107, 173, 255, 663
SatI GCNGC 2 cut(s) 531, 534
Sau3AI GATC 1 cut(s) 19
SchI GAGTC 2 cut(s) 131, 501
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 214, 307, 627
SfoI GGCGCC 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 2 cut(s) 68, 301
SsiI CCGC 1 cut(s) 627
SspDI GGCGCC 1 cut(s) 88
SspMI CTAG 4 cut(s) 44, 56, 246, 554
SstI GAGCTC 1 cut(s) 66
TaaI ACNGT 1 cut(s) 15
TaiI ACGT 1 cut(s) 354
TaqI TCGA 4 cut(s) 66, 409, 495, 621
TasI AATT 2 cut(s) 68, 301
TatI WGTACW 1 cut(s) 182
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 4 cut(s) 107, 173, 255, 663
Tru9I TTAA 4 cut(s) 107, 173, 255, 663
TscAI CASTG 1 cut(s) 124
TseI GCWGC 2 cut(s) 530, 533
TspDTI ATGAA 1 cut(s) 285
TspRI CASTG 1 cut(s) 124
Tth111I GACNNNGTC 1 cut(s) 555
VspI ATTAAT 1 cut(s) 255
XceI RCATGY 1 cut(s) 597
XmiI GTMKAC 1 cut(s) 10
XspI CTAG 4 cut(s) 44, 56, 246, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.