RLG00000035787

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016807
Physical Location & Seq
Reverse (-)
71588502 .. 71609489
20988 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035787

Sequence Viewer

Length: 1728 bp
ATGTCTTCCATAGGTATTGACAAGAAGGAGAAGAAGATGAAAAAGGCGAAAGAGGAACGCGTAGAAACTCCAATTCCGATGACAATTGAGAAGAAGAGCAAGAAAGAGTCGAAGAAGCGAAAGGCTTTGGATATGGACGTGAATGCTGGCGACAAGGAAAACAGGAGCGAGCCCTTGAACAATGCCAAACCCAACAGCGAGTTAGAGGTACAAAAGACTAAAAAGAAATCAAAGAAGGACAAGGTGGAGCAAGAGGAGAAGAAAAAGAACGGGGAGGAGGAGACCCCGAAAGCGGTGTCGATGACCAAAAAGAAATCAAAAAAGGCCAAGGTGGAGCAAAAGGAGAAGAAAAAGAATGTGGAGGAGGAGAAACCTAATGCGGTGTCGCAGTTTCGGATTTCGCAGCCATTGAGGGCTAAGTTGAAGGAGAGGGGGGTCGAATCCTTGTCTCCAATTCAGGCCATGACCTTCGATACCATCTTGGATGGGTTCGACTTGATGGGTCAGGCTAGCGCTGGCCAGGATAAAACTCTGGCTTTTGTGTTACCCATCCTGGAGTCTCTAATAAATGGTTCTGCAGAACAATCTAAAAAGACCGGGTACAGGGCTCCAACTGTTATTGTACTTTTACCCACAAGGGACTTGGCCATACAGGTTCTTAAGGAATTTGGATTTTACGGCGGTTCTTTGGGAATAGTGTCATGCTGTGTATACGGAGGTTCCTCCCACAAGGTTCAAACTAAGAAGTTAAAGAGAGGCCGGGTTGATATCATTGTTGGAACACCTGGCCGTGTAAAGGATCAAATAGAAAAGGGCAACATAGACTTGAGCAATTTGAAGTTTCGGGTCCTTGGTGAAGCTGATGAAATGTCAAAGAAGGGGTTAGTTGAACATGTTGAATTCATTCTAGGCAAGGAATATGATGTAACTAAAGTTCAAACGCTTCTCTTCAGTACTACCATGCCTCATTGGGTGGAAGAGATTGCTCCCTCCAGGTATCTTAAATCAAATTATAAAAAGGTTGATTTGGTTGGTAATGAAAAAATGAAGGCCAGTCAATCCGATGGTGGTTTCTCTGATAAAAGAAATGATAGGTTTTCCATAGCCACAAATGTTGCAGCACATGGACTGGATATCAATGATATTCAATTAATTATTCAGTATGAACCTCCACGTAATGTAGAAGACTATATCCATCGATGTGGATGCACAGGGAGAGCAGTCGAGTCTGTTGCAGAGGAGCTTCTTAACACCTCTGGCCTATATGCAGAAAACTACTGGCAAAGGCCCTTGCTAAGACTGCTCCACCGAGATAAAGAAGAGAGCACGTCTTACCATGTCACTGTACTGCTTGAGTGTGAAAGAGCCCTTTACACCCGGGGGAGGTTCTTGCCTGAGGAGAAGGTTGAGTCACTGAAGGGTATGAAGTTAACAACTGATGGAAAGGGTGCAATGTTTTCAAGTTCGACGAAAGTCGCTAATGTGAACTTAAAGGCGTTGGAAATATTGCCAAATCTACAAGAAAAAGCAAGGGGAGGAAGATTTGGCCCTGGTGATGGATTTGGAAGAAGAGATGGCAGGGTTCATGATTACTCTGAAAGTCTGATAGAAGAAATAATAGGTTTTCCCATAAATCCGATGGGGGTTTCTCTGATAGAAGAAATAATAGTTTTTCCAATCGAGCCTGGAGAGGTTGTGGCTAGTGGCTACTATGGCAACACACGGTGA

Protein Analysis

576

Amino Acids

64.35

Weight (kDa)

9.18

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 150 - 328 2.6e-34 DEAD/DEAH box helicase
Helicase_C PF00271 313 - 407 4.5e-11 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016807)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36510 FvH4_3g36510
pyrus_communis pycom01g19970
rosa_chinensis RchiOBHm_Chr5g0065371 RchiOBHm_Chr5g0065431
rosa_laevigata RLG00000035787
rosa_multiflora Rmu_co8502081.1_g000001
rosa_roxburghii Rroxscaffold_1G00015820
rosa_rugosa Rorug05G0371800
rosa_samantha Rh5AG429500 Rh5BG444800 Rh5CG467100 Rh5DG457300
rosa_wichuraiana Rw5G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1014
AccI GTMKAC 1 cut(s) 711
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 293, 380, 681
AclWI GGATC 1 cut(s) 807
AcoI YGGCCR 3 cut(s) 517, 645, 787
AcsI RAATTY 2 cut(s) 665, 899
AcuI CTGAAG 2 cut(s) 934, 1436
AdeI CACNNNGTG 1 cut(s) 1725
AfaI GTAC 5 cut(s) 210, 602, 624, 955, 1347
AfeI AGCGCT 1 cut(s) 514
AfiI CCNNNNNNNGG 5 cut(s) 292, 603, 796, 1383, 1556
AflII CTTAAG 1 cut(s) 659
AflIII ACRYGT 2 cut(s) 58, 892
AgsI TTSAA 9 cut(s) 178, 424, 737, 838, 890, 899, 938, 1148, 1461
AjiI CACGTC 2 cut(s) 139, 1329
AjnI CCWGG 6 cut(s) 519, 552, 784, 992, 1549, 1684
AluBI AGCT 2 cut(s) 860, 1243
AluI AGCT 2 cut(s) 860, 1243
Alw21I GWGCWC 1 cut(s) 1328
Alw26I GTCTC 3 cut(s) 275, 453, 564
AlwI GGATC 1 cut(s) 807
Ama87I CYCGRG 1 cut(s) 1377
Aor51HI AGCGCT 1 cut(s) 514
ApeKI GCWGC 2 cut(s) 403, 1118
ApoI RAATTY 2 cut(s) 665, 899
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AseI ATTAAT 1 cut(s) 1151
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 3 cut(s) 847, 1287, 1547
AsuC2I CCSGG 4 cut(s) 598, 761, 1378, 1379
AsuHPI GGTGA 2 cut(s) 866, 1565
AsuNHI GCTAGC 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 1377
AvaII GGWCC 1 cut(s) 847
AxyI CCTNAGG 1 cut(s) 1395
BalI TGGCCA 2 cut(s) 519, 647
BanII GRGCYC 3 cut(s) 174, 610, 1369
BbsI GAAGAC 1 cut(s) 1191
Bbv12I GWGCWC 1 cut(s) 1328
BbvI GCAGC 2 cut(s) 415, 1130
BceAI ACGGC 2 cut(s) 694, 774
BcgI CGANNNNNNTGC 4 cut(s) 1188, 1214, 1222, 1248
BciT130I CCWGG 6 cut(s) 521, 554, 786, 994, 1551, 1686
BcnI CCSGG 4 cut(s) 598, 761, 1378, 1379
BcoDI GTCTC 3 cut(s) 275, 453, 564
BfaI CTAG 3 cut(s) 510, 908, 1701
BfmI CTRYAG 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 516
BfrI CTTAAG 1 cut(s) 659
BisI GCNGC 2 cut(s) 404, 1119
BlsI GCNGC 2 cut(s) 405, 1120
BmcAI AGTACT 1 cut(s) 955
Bme18I GGWCC 1 cut(s) 847
BmeT110I CYCGRG 1 cut(s) 1377
BmgBI CACGTC 2 cut(s) 139, 1329
BmgT120I GGNCC 3 cut(s) 847, 1287, 1547
BmiI GGNNCC 3 cut(s) 609, 721, 848
BmsI GCATC 1 cut(s) 1196
BmtI GCTAGC 1 cut(s) 513
BoxI GACNNNNGTC 1 cut(s) 1472
BpiI GAAGAC 1 cut(s) 1191
BpmI CTGGAG 3 cut(s) 575, 976, 1707
BpuEI CTTGAG 2 cut(s) 847, 1373
BpuMI CCSGG 4 cut(s) 598, 761, 1378, 1379
Bsa29I ATCGAT 1 cut(s) 1198
BsaAI YACGTR 1 cut(s) 1175
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 5 cut(s) 327, 850, 1377, 1378, 1549
BsaXI ACNNNNNCTCC 2 cut(s) 1680, 1710
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 603, 796, 1383, 1556
Bse1I ACTGG 3 cut(s) 1053, 1134, 1283
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 1 cut(s) 1458
BseBI CCWGG 6 cut(s) 521, 554, 786, 994, 1551, 1686
BseCI ATCGAT 1 cut(s) 1198
BseDI CCNNGG 5 cut(s) 327, 850, 1377, 1378, 1549
BseGI GGATG 3 cut(s) 490, 549, 1211
BseLI CCNNNNNNNGG 5 cut(s) 292, 603, 796, 1383, 1556
BseMI GCAATG 1 cut(s) 1458
BseMII CTCAG 1 cut(s) 1386
BseNI ACTGG 3 cut(s) 1053, 1134, 1283
BseRI GAGGAG 7 cut(s) 269, 290, 293, 377, 380, 1253, 1412
BseXI GCAGC 2 cut(s) 415, 1130
Bsh1236I CGCG 1 cut(s) 60
BshVI ATCGAT 1 cut(s) 1198
BsiHKAI GWGCWC 1 cut(s) 1328
BsiHKCI CYCGRG 1 cut(s) 1377
BsiSI CCGG 3 cut(s) 597, 760, 1378
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 5 cut(s) 292, 603, 796, 1383, 1556
BsmAI GTCTC 3 cut(s) 275, 453, 564
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 148
Bso31I GGTCTC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 1377
Bsp1286I GDGCHC 4 cut(s) 174, 610, 1328, 1369
Bsp143I GATC 1 cut(s) 799
BspACI CCGC 3 cut(s) 293, 380, 681
BspCNI CTCAG 1 cut(s) 1387
BspDI ATCGAT 1 cut(s) 1198
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 1585
BspLI GGNNCC 3 cut(s) 609, 721, 848
BspMAI CTGCAG 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 513
BspPI GGATC 1 cut(s) 807
BspQI GCTCTTC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 1458
BsrI ACTGG 3 cut(s) 1053, 1134, 1283
BssECI CCNNGG 5 cut(s) 327, 850, 1377, 1378, 1549
BssMI GATC 1 cut(s) 799
BssNAI GTATAC 1 cut(s) 712
BssT1I CCWWGG 2 cut(s) 327, 850
Bst1107I GTATAC 1 cut(s) 712
Bst2UI CCWGG 6 cut(s) 521, 554, 786, 994, 1551, 1686
Bst4CI ACNGT 3 cut(s) 616, 1345, 1725
Bst6I CTCTTC 5 cut(s) 89, 953, 972, 1314, 1564
BstAFI CTTAAG 1 cut(s) 659
BstBAI YACGTR 1 cut(s) 1175
BstC8I GCNNGC 4 cut(s) 148, 170, 511, 517
BstDEI CTNAG 4 cut(s) 417, 741, 1295, 1395
BstF5I GGATG 3 cut(s) 490, 549, 1211
BstFNI CGCG 1 cut(s) 60
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 1 cut(s) 802
BstMAI GTCTC 3 cut(s) 275, 453, 564
BstMBI GATC 1 cut(s) 799
BstMWI GCNNNNNNNGC 2 cut(s) 1300, 1713
BstNI CCWGG 6 cut(s) 521, 554, 786, 994, 1551, 1686
BstNSI RCATGY 1 cut(s) 896
BstPAI GACNNNNGTC 1 cut(s) 1472
BstSFI CTRYAG 1 cut(s) 576
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 415, 1130
BstV2I GAAGAC 1 cut(s) 1191
BstXI CCANNNNNNTGG 1 cut(s) 1202
BstZ17I GTATAC 1 cut(s) 712
Bsu15I ATCGAT 1 cut(s) 1198
Bsu36I CCTNAGG 1 cut(s) 1395
BsuTUI ATCGAT 1 cut(s) 1198
BtrI CACGTC 2 cut(s) 139, 1329
BtsCI GGATG 3 cut(s) 490, 549, 1211
BtsIMutI CAGTG 2 cut(s) 1341, 1412
Cac8I GCNNGC 4 cut(s) 148, 170, 511, 517
CciI TCATGA 1 cut(s) 1585
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 3 cut(s) 847, 1287, 1547
Cfr9I CCCGGG 1 cut(s) 1377
ClaI ATCGAT 1 cut(s) 1198
Csp6I GTAC 5 cut(s) 209, 601, 623, 954, 1346
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
CviAII CATG 7 cut(s) 463, 702, 893, 961, 1124, 1337, 1586
CviQI GTAC 5 cut(s) 209, 601, 623, 954, 1346
DdeI CTNAG 4 cut(s) 417, 741, 1295, 1395
DpnI GATC 1 cut(s) 801
DpnII GATC 1 cut(s) 799
DraIII CACNNNGTG 1 cut(s) 1725
EaeI YGGCCR 3 cut(s) 517, 645, 787
Eam1104I CTCTTC 5 cut(s) 89, 953, 972, 1314, 1564
EarI CTCTTC 5 cut(s) 89, 953, 972, 1314, 1564
Eco130I CCWWGG 2 cut(s) 327, 850
Eco24I GRGCYC 3 cut(s) 174, 610, 1369
Eco31I GGTCTC 1 cut(s) 275
Eco32I GATATC 2 cut(s) 769, 1135
Eco47I GGWCC 1 cut(s) 847
Eco47III AGCGCT 1 cut(s) 514
Eco57I CTGAAG 2 cut(s) 934, 1436
Eco81I CCTNAGG 1 cut(s) 1395
Eco88I CYCGRG 1 cut(s) 1377
EcoO109I RGGNCCY 2 cut(s) 847, 1287
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 6 cut(s) 519, 552, 784, 992, 1549, 1684
EcoRV GATATC 2 cut(s) 769, 1135
EcoT14I CCWWGG 2 cut(s) 327, 850
EcoT38I GRGCYC 3 cut(s) 174, 610, 1369
ErhI CCWWGG 2 cut(s) 327, 850
FaeI CATG 7 cut(s) 466, 705, 896, 964, 1127, 1340, 1589
FaqI GGGAC 1 cut(s) 653
FatI CATG 7 cut(s) 462, 701, 892, 960, 1123, 1336, 1585
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 2 cut(s) 404, 1119
FokI GGATG 3 cut(s) 497, 536, 1218
FriOI GRGCYC 3 cut(s) 174, 610, 1369
Fsp4HI GCNGC 2 cut(s) 404, 1119
FspBI CTAG 3 cut(s) 510, 908, 1701
GlaI GCGC 1 cut(s) 514
GluI GCNGC 2 cut(s) 404, 1119
GsuI CTGGAG 3 cut(s) 575, 976, 1707
HaeII RGCGCY 1 cut(s) 516
HapII CCGG 3 cut(s) 597, 760, 1378
HhaI GCGC 1 cut(s) 515
Hin1II CATG 7 cut(s) 466, 705, 896, 964, 1127, 1340, 1589
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 1 cut(s) 1431
HindII GTYRAC 1 cut(s) 1431
HinfI GANTC 5 cut(s) 107, 440, 557, 1226, 1409
HpaI GTTAAC 1 cut(s) 1431
HpaII CCGG 3 cut(s) 597, 760, 1378
HphI GGTGA 2 cut(s) 866, 1565
Hpy166II GTNNAC 3 cut(s) 712, 1431, 1486
Hpy188I TCNGA 8 cut(s) 78, 396, 1063, 1078, 1597, 1605, 1638, 1653
Hpy188III TCNNGA 1 cut(s) 1586
Hpy8I GTNNAC 3 cut(s) 712, 1431, 1486
Hpy99I CGWCG 1 cut(s) 1471
HpyAV CCTTC 8 cut(s) 19, 229, 418, 478, 871, 1042, 1396, 1411
HpyCH4III ACNGT 3 cut(s) 616, 1345, 1725
HpyCH4IV ACGT 3 cut(s) 138, 1174, 1328
HpyCH4V TGCA 6 cut(s) 578, 1118, 1209, 1235, 1268, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 1300, 1713
HpyF3I CTNAG 4 cut(s) 417, 741, 1295, 1395
HpySE526I ACGT 3 cut(s) 138, 1174, 1328
Hsp92II CATG 7 cut(s) 466, 705, 896, 964, 1127, 1340, 1589
HspAI GCGC 1 cut(s) 513
KspAI GTTAAC 1 cut(s) 1431
Kzo9I GATC 1 cut(s) 799
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 7 cut(s) 165, 247, 334, 613, 991, 1240, 1308
Lsp1109I GCAGC 2 cut(s) 415, 1130
LweI GCATC 1 cut(s) 1196
MaeI CTAG 3 cut(s) 510, 908, 1701
MaeII ACGT 3 cut(s) 138, 1174, 1328
MaeIII GTNAC 4 cut(s) 543, 925, 1339, 1410
MalI GATC 1 cut(s) 801
MboI GATC 1 cut(s) 799
MfeI CAATTG 1 cut(s) 84
MhlI GDGCHC 4 cut(s) 174, 610, 1328, 1369
MlsI TGGCCA 2 cut(s) 519, 647
MluCI AATT 9 cut(s) 72, 84, 453, 665, 832, 899, 1009, 1148, 1152
MluI ACGCGT 1 cut(s) 58
MluNI TGGCCA 2 cut(s) 519, 647
MlyI GAGTC 4 cut(s) 116, 566, 1235, 1418
MmeI TCCRAC 3 cut(s) 635, 757, 1479
Mox20I TGGCCA 2 cut(s) 519, 647
MroXI GAANNNNTTC 1 cut(s) 903
MscI TGGCCA 2 cut(s) 519, 647
MseI TTAA 7 cut(s) 660, 749, 1002, 1151, 1248, 1430, 1490
MslI CAYNNNNRTG 1 cut(s) 1200
Msp20I TGGCCA 2 cut(s) 519, 647
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 3 cut(s) 597, 760, 1378
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 6 cut(s) 521, 554, 786, 994, 1551, 1686
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 2 cut(s) 1300, 1713
NciI CCSGG 4 cut(s) 598, 761, 1378, 1379
NdeII GATC 1 cut(s) 799
NheI GCTAGC 1 cut(s) 509
NlaIII CATG 7 cut(s) 466, 705, 896, 964, 1127, 1340, 1589
NlaIV GGNNCC 3 cut(s) 609, 721, 848
NmuCI GTSAC 2 cut(s) 1339, 1410
NspI RCATGY 1 cut(s) 896
PagI TCATGA 1 cut(s) 1585
PciI ACATGT 1 cut(s) 892
PciSI GCTCTTC 1 cut(s) 89
PctI GAATGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 1 cut(s) 440
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 2 cut(s) 405, 1120
PleI GAGTC 4 cut(s) 115, 565, 1234, 1417
PpsI GAGTC 4 cut(s) 115, 565, 1234, 1417
Ppu21I YACGTR 1 cut(s) 1175
PpuMI RGGWCCY 1 cut(s) 847
PscI ACATGT 1 cut(s) 892
PshAI GACNNNNGTC 1 cut(s) 1472
PshBI ATTAAT 1 cut(s) 1151
PsiI TTATAA 1 cut(s) 1014
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 6 cut(s) 519, 552, 784, 992, 1549, 1684
PspGI CCWGG 6 cut(s) 519, 552, 784, 992, 1549, 1684
PspN4I GGNNCC 3 cut(s) 609, 721, 848
PspPI GGNCC 3 cut(s) 847, 1287, 1547
PspPPI RGGWCCY 1 cut(s) 847
PsrI GAACNNNNNNTAC 2 cut(s) 918, 950
PstI CTGCAG 1 cut(s) 580
RsaI GTAC 5 cut(s) 210, 602, 624, 955, 1347
RsaNI GTAC 5 cut(s) 209, 601, 623, 954, 1346
RseI CAYNNNNRTG 1 cut(s) 1200
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 7 cut(s) 660, 749, 1002, 1151, 1248, 1430, 1490
SatI GCNGC 2 cut(s) 404, 1119
Sau3AI GATC 1 cut(s) 799
Sau96I GGNCC 3 cut(s) 847, 1287, 1547
ScaI AGTACT 1 cut(s) 955
SchI GAGTC 4 cut(s) 116, 566, 1235, 1418
SduI GDGCHC 4 cut(s) 174, 610, 1328, 1369
SfaNI GCATC 1 cut(s) 1196
SfcI CTRYAG 1 cut(s) 576
SinI GGWCC 1 cut(s) 847
SmaI CCCGGG 1 cut(s) 1379
SmiMI CAYNNNNRTG 1 cut(s) 1200
SmlI CTYRAG 3 cut(s) 659, 826, 1352
SmoI CTYRAG 3 cut(s) 659, 826, 1352
Sse9I AATT 9 cut(s) 72, 84, 453, 665, 832, 899, 1009, 1148, 1152
SsiI CCGC 3 cut(s) 293, 380, 681
SspI AATATT 1 cut(s) 1506
SspMI CTAG 3 cut(s) 510, 908, 1701
StyI CCWWGG 2 cut(s) 327, 850
TaaI ACNGT 3 cut(s) 616, 1345, 1725
TaiI ACGT 3 cut(s) 141, 1177, 1331
TaqI TCGA 9 cut(s) 110, 299, 438, 471, 492, 1198, 1224, 1466, 1680
TasI AATT 9 cut(s) 72, 84, 453, 665, 832, 899, 1009, 1148, 1152
TatI WGTACW 3 cut(s) 622, 953, 1345
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 7 cut(s) 660, 749, 1002, 1151, 1248, 1430, 1490
Tru9I TTAA 7 cut(s) 660, 749, 1002, 1151, 1248, 1430, 1490
TscAI CASTG 2 cut(s) 1348, 1419
TseFI GTSAC 2 cut(s) 1339, 1410
TseI GCWGC 2 cut(s) 403, 1118
Tsp45I GTSAC 2 cut(s) 1339, 1410
TspDTI ATGAA 8 cut(s) 53, 879, 892, 1053, 1061, 1179, 1439, 1574
TspGWI ACGGA 1 cut(s) 729
TspMI CCCGGG 1 cut(s) 1377
TspRI CASTG 2 cut(s) 1348, 1419
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 847
VspI ATTAAT 1 cut(s) 1151
XapI RAATTY 2 cut(s) 665, 899
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 2 cut(s) 640, 1636
XmaI CCCGGG 1 cut(s) 1377
XmiI GTMKAC 1 cut(s) 711
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 3 cut(s) 510, 908, 1701
ZrmI AGTACT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.