Rh5DG457300

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016807
Physical Location & Seq
Reverse (-)
72719077 .. 72721260
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG457300.1

Sequence Viewer

Length: 1146 bp
ATGTCTTCCATAGGTATTGACAAGAAGGAGAAGAAGATGAAAAAGGCGAAAGAGGAACGCGTAGAAATTCCGATTCCGATGAGAATTGAGAAGAAGAGCAAGAAAGAGTCAAAGAAGCGAAAGGATTTGGATATGGACGTGAATGCTGGCGACAAGGAAAACAGGAGCGAGCCCTTGAACAATGCCAAATCCAACAGCGAGTTTGAGGTACAAAAGACTAAGAAGAAATCAAAGAAGGACAAGGTGGAGCAAGAGGAGAAGAAAACGAACGGGGAGGAGGAGACCCCGAAAGCGGTGTCGATGACCAAAAAGAAATCAAAGAAGGCCAAGGTGGAGGAAGAGGAGAAGAAAAAGAATGTGGAGGAGGAGAAACCTAATGCGGTGTCGCAGTTTCGGATTTCGCAGCCATTGAGGGCTAAGTTGAAGGAGAGGGGGGTCGAGTCCTTGTCTCCAATTCAGGCCATGACCTTCGATACCATCTTGGATGGGTTCGACTTGATGGGTCAGGCTAGCGCTGGCCAGGATAAAACTCTGGCTTTTGTGTTACCCATCGTGGAGTCTCTAATAAATGGTTCTGCAGAACAATCTAAAAAGACCGGGTACAGGGCTCCAACTGTTATCGTACTTTTACCTACAAGGGACTTGGCCATACAGGTTCTTAAGGAATTTGGATTTTACGGCGGTTCTTTGGGAATAGTGTCATGTTGTGTACACGGAGGATCCTCCCACAAGGTTCAAACAAAGAAGTTAAAGAGAGGCCGGGTTGATATCATTGTTGGAACACCTGGCCGTGTAAAGGATCAAATAGAAAAGGGCAACATAGACTTGAGCAATTTGAAGTTTCGGGTCCTTGGTGAAGCTGATGAAATGTCAAAGAAGGGGTTAGTTGAACATGTTGAATTCATTCTAGGCAAGGAATATGATGTAGCTAAAGTTCAAACGCTTCTCTTCAGTACTACCATGTCCCATTGGGTGGAAGAGATTGCTCCCTCCAGGTATCTTAAATCAAATTATAAAAAGGTTGATTTGGTTGGTAATGAAAAAATGAAGACCAGTCAATCCGATGGTGGTTTCTCTGATACAAGAAATGATAGGTTTTCCGGTCAAGGTGGGACAGGTCGCAGCAACTATGGCAACAGATGGTGA

Protein Analysis

381

Amino Acids

42.68

Weight (kDa)

9.54

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 150 - 328 2.1e-33 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016807)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36510 FvH4_3g36510
pyrus_communis pycom01g19970
rosa_chinensis RchiOBHm_Chr5g0065371 RchiOBHm_Chr5g0065431
rosa_laevigata RLG00000035787
rosa_multiflora Rmu_co8502081.1_g000001
rosa_roxburghii Rroxscaffold_1G00015820
rosa_rugosa Rorug05G0371800
rosa_samantha Rh5AG429500 Rh5BG444800 Rh5CG467100 Rh5DG457300
rosa_wichuraiana Rw5G040320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1014
AccB7I CCANNNNNTGG 1 cut(s) 973
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 293, 380, 681
AclWI GGATC 3 cut(s) 714, 727, 807
AcoI YGGCCR 3 cut(s) 517, 645, 787
AcsI RAATTY 3 cut(s) 66, 665, 899
AcuI CTGAAG 1 cut(s) 934
AfaI GTAC 5 cut(s) 210, 602, 624, 711, 955
AfeI AGCGCT 1 cut(s) 514
AfiI CCNNNNNNNGG 4 cut(s) 292, 603, 796, 973
AflII CTTAAG 1 cut(s) 659
AflIII ACRYGT 2 cut(s) 58, 892
AgsI TTSAA 7 cut(s) 178, 424, 737, 838, 890, 899, 938
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 3 cut(s) 519, 784, 992
AluBI AGCT 2 cut(s) 860, 929
AluI AGCT 2 cut(s) 860, 929
Alw26I GTCTC 3 cut(s) 275, 453, 564
AlwI GGATC 3 cut(s) 714, 727, 807
Aor51HI AGCGCT 1 cut(s) 514
AoxI GGCC 6 cut(s) 324, 459, 517, 645, 757, 787
ApeKI GCWGC 2 cut(s) 403, 1122
ApoI RAATTY 3 cut(s) 66, 665, 899
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 1 cut(s) 847
AsuC2I CCSGG 2 cut(s) 598, 761
AsuHPI GGTGA 1 cut(s) 866
AsuNHI GCTAGC 1 cut(s) 509
AvaII GGWCC 1 cut(s) 847
BalI TGGCCA 2 cut(s) 519, 647
BamHI GGATCC 1 cut(s) 719
BanII GRGCYC 2 cut(s) 174, 610
BbsI GAAGAC 1 cut(s) 1055
BbvI GCAGC 2 cut(s) 415, 1134
BccI CCATC 6 cut(s) 479, 485, 493, 557, 1058, 1134
BceAI ACGGC 2 cut(s) 694, 774
BciT130I CCWGG 3 cut(s) 521, 786, 994
BcnI CCSGG 2 cut(s) 598, 761
BcoDI GTCTC 3 cut(s) 275, 453, 564
BfaI CTAG 2 cut(s) 510, 908
BfmI CTRYAG 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 516
BfrI CTTAAG 1 cut(s) 659
BisI GCNGC 2 cut(s) 404, 1123
BlsI GCNGC 2 cut(s) 405, 1124
BmcAI AGTACT 1 cut(s) 955
Bme1390I CCNGG 5 cut(s) 521, 598, 761, 786, 994
Bme18I GGWCC 1 cut(s) 847
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 847
BmiI GGNNCC 3 cut(s) 609, 721, 848
BmrFI CCNGG 5 cut(s) 521, 598, 761, 786, 994
BmtI GCTAGC 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 1055
BpmI CTGGAG 1 cut(s) 976
BpuEI CTTGAG 1 cut(s) 847
BpuMI CCSGG 2 cut(s) 598, 761
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 327, 850
BsaWI WCCGGW 1 cut(s) 1100
Bsc4I CCNNNNNNNGG 4 cut(s) 292, 603, 796, 973
Bse1I ACTGG 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 521, 786, 994
BseDI CCNNGG 2 cut(s) 327, 850
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 4 cut(s) 292, 603, 796, 973
BseNI ACTGG 1 cut(s) 1053
BseRI GAGGAG 6 cut(s) 269, 290, 293, 356, 377, 380
BseXI GCAGC 2 cut(s) 415, 1134
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 6 cut(s) 326, 461, 519, 647, 759, 789
BsiSI CCGG 3 cut(s) 597, 760, 1101
BslFI GGGAC 3 cut(s) 653, 949, 1126
BslI CCNNNNNNNGG 4 cut(s) 292, 603, 796, 973
BsmAI GTCTC 3 cut(s) 275, 453, 564
BsmFI GGGAC 3 cut(s) 653, 949, 1126
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 6 cut(s) 326, 461, 519, 647, 759, 789
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 174, 610
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 2 cut(s) 719, 799
BspACI CCGC 3 cut(s) 293, 380, 681
BspANI GGCC 6 cut(s) 326, 461, 519, 647, 759, 789
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 609, 721, 848
BspMAI CTGCAG 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 513
BspPI GGATC 3 cut(s) 714, 727, 807
BspQI GCTCTTC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 1 cut(s) 1053
BssECI CCNNGG 2 cut(s) 327, 850
BssMI GATC 2 cut(s) 719, 799
BssT1I CCWWGG 2 cut(s) 327, 850
Bst2UI CCWGG 3 cut(s) 521, 786, 994
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 4 cut(s) 89, 333, 953, 972
BstAFI CTTAAG 1 cut(s) 659
BstAUI TGTACA 1 cut(s) 709
BstC8I GCNNGC 4 cut(s) 148, 170, 511, 517
BstDEI CTNAG 2 cut(s) 219, 417
BstF5I GGATG 1 cut(s) 490
BstFNI CGCG 1 cut(s) 60
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 2 cut(s) 722, 802
BstMAI GTCTC 3 cut(s) 275, 453, 564
BstMBI GATC 2 cut(s) 719, 799
BstMWI GCNNNNNNNGC 1 cut(s) 1131
BstNI CCWGG 3 cut(s) 521, 786, 994
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 5 cut(s) 519, 596, 759, 784, 992
BstSFI CTRYAG 1 cut(s) 576
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 415, 1134
BstV2I GAAGAC 1 cut(s) 1055
BstX2I RGATCY 1 cut(s) 719
BstYI RGATCY 1 cut(s) 719
BsuRI GGCC 6 cut(s) 326, 461, 519, 647, 759, 789
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 490
Cac8I GCNNGC 4 cut(s) 148, 170, 511, 517
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 847
Csp6I GTAC 5 cut(s) 209, 601, 623, 710, 954
CviAII CATG 4 cut(s) 463, 702, 893, 961
CviQI GTAC 5 cut(s) 209, 601, 623, 710, 954
DdeI CTNAG 2 cut(s) 219, 417
DpnI GATC 2 cut(s) 721, 801
DpnII GATC 2 cut(s) 719, 799
EaeI YGGCCR 3 cut(s) 517, 645, 787
Eam1104I CTCTTC 4 cut(s) 89, 333, 953, 972
EarI CTCTTC 4 cut(s) 89, 333, 953, 972
Eco130I CCWWGG 2 cut(s) 327, 850
Eco24I GRGCYC 2 cut(s) 174, 610
Eco31I GGTCTC 1 cut(s) 275
Eco32I GATATC 1 cut(s) 769
Eco47I GGWCC 1 cut(s) 847
Eco47III AGCGCT 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 934
EcoO109I RGGNCCY 1 cut(s) 847
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 3 cut(s) 519, 784, 992
EcoRV GATATC 1 cut(s) 769
EcoT14I CCWWGG 2 cut(s) 327, 850
EcoT38I GRGCYC 2 cut(s) 174, 610
ErhI CCWWGG 2 cut(s) 327, 850
FaeI CATG 4 cut(s) 466, 705, 896, 964
FaqI GGGAC 3 cut(s) 653, 949, 1126
FatI CATG 4 cut(s) 462, 701, 892, 960
Fnu4HI GCNGC 2 cut(s) 404, 1123
FokI GGATG 1 cut(s) 497
FriOI GRGCYC 2 cut(s) 174, 610
Fsp4HI GCNGC 2 cut(s) 404, 1123
FspBI CTAG 2 cut(s) 510, 908
GlaI GCGC 1 cut(s) 514
GluI GCNGC 2 cut(s) 404, 1123
GsuI CTGGAG 1 cut(s) 976
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 6 cut(s) 326, 461, 519, 647, 759, 789
HapII CCGG 3 cut(s) 597, 760, 1101
HhaI GCGC 1 cut(s) 515
Hin1II CATG 4 cut(s) 466, 705, 896, 964
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HinfI GANTC 4 cut(s) 73, 107, 440, 557
HpaII CCGG 3 cut(s) 597, 760, 1101
HphI GGTGA 1 cut(s) 866
Hpy166II GTNNAC 2 cut(s) 710, 712
Hpy188I TCNGA 5 cut(s) 72, 78, 396, 1063, 1078
Hpy8I GTNNAC 2 cut(s) 710, 712
HpyAV CCTTC 6 cut(s) 19, 229, 316, 418, 478, 871
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 1131
HpyF3I CTNAG 2 cut(s) 219, 417
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 4 cut(s) 466, 705, 896, 964
HspAI GCGC 1 cut(s) 513
Kzo9I GATC 2 cut(s) 719, 799
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 4 cut(s) 165, 247, 613, 991
Lsp1109I GCAGC 2 cut(s) 415, 1134
MaeI CTAG 2 cut(s) 510, 908
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 543
MalI GATC 2 cut(s) 721, 801
MboI GATC 2 cut(s) 719, 799
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 2 cut(s) 174, 610
MlsI TGGCCA 2 cut(s) 519, 647
MluCI AATT 7 cut(s) 66, 84, 453, 665, 832, 899, 1009
MluI ACGCGT 1 cut(s) 58
MluNI TGGCCA 2 cut(s) 519, 647
MlyI GAGTC 3 cut(s) 116, 449, 566
MmeI TCCRAC 3 cut(s) 216, 635, 757
Mox20I TGGCCA 2 cut(s) 519, 647
MroXI GAANNNNTTC 1 cut(s) 903
MscI TGGCCA 2 cut(s) 519, 647
MseI TTAA 3 cut(s) 660, 749, 1002
Msp20I TGGCCA 2 cut(s) 519, 647
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 3 cut(s) 597, 760, 1101
MspR9I CCNGG 5 cut(s) 521, 598, 761, 786, 994
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 3 cut(s) 521, 786, 994
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 1 cut(s) 1131
NciI CCSGG 2 cut(s) 598, 761
NdeII GATC 2 cut(s) 719, 799
NheI GCTAGC 1 cut(s) 509
NlaIII CATG 4 cut(s) 466, 705, 896, 964
NlaIV GGNNCC 3 cut(s) 609, 721, 848
NspI RCATGY 1 cut(s) 896
PciI ACATGT 1 cut(s) 892
PciSI GCTCTTC 1 cut(s) 89
PctI GAATGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 1 cut(s) 73
PflMI CCANNNNNTGG 1 cut(s) 973
PkrI GCNGC 2 cut(s) 405, 1124
PleI GAGTC 3 cut(s) 115, 448, 565
PpsI GAGTC 3 cut(s) 115, 448, 565
PpuMI RGGWCCY 1 cut(s) 847
PscI ACATGT 1 cut(s) 892
PsiI TTATAA 1 cut(s) 1014
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 3 cut(s) 519, 784, 992
PspGI CCWGG 3 cut(s) 519, 784, 992
PspN4I GGNNCC 3 cut(s) 609, 721, 848
PspPI GGNCC 1 cut(s) 847
PspPPI RGGWCCY 1 cut(s) 847
PsrI GAACNNNNNNTAC 2 cut(s) 918, 950
PstI CTGCAG 1 cut(s) 580
PsuI RGATCY 1 cut(s) 719
RsaI GTAC 5 cut(s) 210, 602, 624, 711, 955
RsaNI GTAC 5 cut(s) 209, 601, 623, 710, 954
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 3 cut(s) 660, 749, 1002
SatI GCNGC 2 cut(s) 404, 1123
Sau3AI GATC 2 cut(s) 719, 799
Sau96I GGNCC 1 cut(s) 847
ScaI AGTACT 1 cut(s) 955
SchI GAGTC 3 cut(s) 116, 449, 566
ScrFI CCNGG 5 cut(s) 521, 598, 761, 786, 994
SduI GDGCHC 2 cut(s) 174, 610
SfcI CTRYAG 1 cut(s) 576
SinI GGWCC 1 cut(s) 847
SmlI CTYRAG 2 cut(s) 659, 826
SmoI CTYRAG 2 cut(s) 659, 826
Sse9I AATT 7 cut(s) 66, 84, 453, 665, 832, 899, 1009
SsiI CCGC 3 cut(s) 293, 380, 681
SspMI CTAG 2 cut(s) 510, 908
StyD4I CCNGG 5 cut(s) 519, 596, 759, 784, 992
StyI CCWWGG 2 cut(s) 327, 850
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 1 cut(s) 141
TaqI TCGA 4 cut(s) 299, 438, 471, 492
TasI AATT 7 cut(s) 66, 84, 453, 665, 832, 899, 1009
TatI WGTACW 2 cut(s) 709, 953
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 3 cut(s) 660, 749, 1002
Tru9I TTAA 3 cut(s) 660, 749, 1002
TseI GCWGC 2 cut(s) 403, 1122
TspDTI ATGAA 5 cut(s) 53, 879, 892, 1053, 1061
TspGWI ACGGA 1 cut(s) 729
Van91I CCANNNNNTGG 1 cut(s) 973
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 847
XapI RAATTY 3 cut(s) 66, 665, 899
XceI RCATGY 1 cut(s) 896
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 510, 908
ZrmI AGTACT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.