Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0014564

Physical Location & Seq

Genomic Coordinates Forward (+)

76125941 .. 76130914

Gene Structure 4974 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000036127

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGAACCCTTCTGAAGAACTACTGGAACAAGGAATTTCTCCAAGGAACTTGCAGAGGGTATCTTGTGCGACTGTGCTCACATTGGCTTATCAAAGTCTGGGAGTCATTTATGGAGACCTCAGCATATCCCCTCTATATGTTTACAAGACCACCTTCTCCGGAAAGTTGAGTCTTCATGAGGACGATGAAGAGATTTTCGGGGTGCTTTCATTTATTTTATGGACATTTTCTCTCATTGCTCTCTTCAAGTATGTTTTCATTGTGATGTTGGCTGATGACAATGGTGAAGGTGGTACCTTTGCCTTATACTCCCTCCTCTGTCGTCACGCAAGATTAAGCACTCTACCTAATCAAGAAATCACAGATGAGGAACTGTCTGAATATGTGACAGAGGGAAACAATGATACATGGCATAGTGCAGCTCTGAAATCATGCTTCGACAAGCACCCAAGATTTCAAAAAGGGCTGCTGATTTTTGTTCTGTTTGGGACCTGTATGGCAATTGGTGATGGTGTACTCACTCCGTCAATATCAGTTCTTTCAGCAGTGTCAGGGGTAAAGCTTAAGTTCACAGAACTCCATGACAATTATGTTGTTATCATCTCATGTGTCTTGCTGGTGGGGCTTTTCTCCCTTCAGCATCACGGAACGCACAGAGTGGCTTTCATGTTTGCTCCAATCATTACTGTATGGCTTCTGTGCATAAGTGGCATTGGAATATACAACATAGTTCAGTGGAATCAATGCGTATTTCAAGCACTCTCTCCAGTTTACATGTTGAAGTTCCTTAGAACCACAGGCATCGAAGGATGGCTATCGTTAGCTGGTGTTGTTCTTTCAATAACAGGTGTAGAGGCAATGTTTGCTAACTTGGGCCATTTCTCTTCACTCTCAATAAAGATAGCCTTCACATGTTTAGTATATCCTTCTCTTGTTCTTTCCTACATGGGCGAGGCTGCATTTCTTTCCAAGCACCATGAAGACATCCAAAGAAGTTTCTACAAATCCATACCAGAAGCTGTGTTTTGGCCGGTGTTTATACTCGCTAGTTTTGCATCTGTGGTAGGAAGCCAGGCAGTAATTTCAGCTACCTTCTCCATTATAAGCCAGTGTTCTGCGCTGAAGTGCTTTCCACGCGTCAAGATAGTTCATACTTCGAGTAAAATATATGGGCAAATATACATTCCAGAAGTCAATTGGATGTTAATGTGCCTTTGTTTAGCTGTGACAATTGGATTGAGGGATACTAACATGTTGGGTCATGCATATGGGTTGGCAGTGACCACTGTAATGTTTGTGACGACTTGCTTGATGGCTCTGGTGATGATAATTGTCTGGAAACGAAGGGTAGTTAGTGCATTAGCATTTTTAATGTTCTTTGGATCAATTGAGTTGATTTATATTTCAGCATCTGTCACGAAGATCCCCGAAGGTGGATGGATCCCGGTTCTGCTGTCTCTTGTCTTCATGGCTATAATGTACACTTGGAACTACGGGACAATGATGAAACACCAATTTGATGTTGAGAACAAGGTCTCAATTAACCGAATAGTGTGCTTAGGGCCTAGTCTGGGCATGGTTCGTGTCTCCGGAATTGGACTTGTGTACACTAATTTGGTGACTGGGGTACCAGCTGTTTTCGGACACTTTGTCACTAATCTACCTGCATTCCATAAGGTGCTGATTTTTGTTTGTGTCAGATCCGTTCAAGTTCCCCATGTTAGTGGAAAGGAAAGGTTGATCATTAGTAGGGTTGGCCCGAAGGAGTGTGGCATGTTCAGGTGCATTGTGAGGTATGGTTACAAGGATCTTCAACAGGAAAACTGTAATTTCGAGAATAGGTTGGTGTCTGGTATAGTGCAGTTTGTAGAAACAGAAGACTGTCCTGATGCTGAAGCCTTTTATGCCCCGGATGATCCGGATCAGACTTCCATGGAGTTGAAGAATGAAGAAACATTTTCACGTAATCTTCAAGATGAGTCACTGCAGATATTAGAAGCCAAAGAATCTGGAGTGACTTATATTCTTGGGCATTCACATGCAAAGGCGAAGAAATCAGCATCCTTTATCAAGAAAGTTGCTATAGATATTGTGTATAATTTTATGTGCAAGAATTGTAGAGAGCCTGATGTTGTGTTGAATGCGCCTCCTACTTCTTTGCTAGAAGATCCTAGATCCGGGTCCTCTTCCATCCTCCCGGCCCGACCTGCCCTTCTTCAGCCCAACCCAAAATCCAGGTCCAGCAATAGACCTGCATCCCAGCCAGTGCCCCACTCCTACTCTTGGCCGTTGTCCACCATTGCTGCACCGGCCAACTGCCAGGAACCAAGTCAAGCTCGCGACTGCTACATGGTACCGCTGTGCCTGCAGCTCCACTTCGACTCATCCTGGATCTATGCTCTGCCGCCCTCCATTGCCACACGCGACGACAACCGTGAATCGAGAGCACAGTTTGTTCTCCACGGCCGCATAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

826

Amino Acids

91.65

Weight (kDa)

6.74

Isoelectric Point (pI)

39.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	28 - 517	7.7e-162	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	530 - 722	2e-22	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30070 AT2G30070
fragaria_vesca	FvH4_3g39040
malus_domestica	MD11G1069200.v1.1
prunus_persica	Prupe.6G054100_v2.0.a1 Prupe.6G054100_v2.0.a1
pyrus_communis	pycom11g05770
rosa_chinensis	RchiOBHm_Chr5g0070351
rosa_laevigata	RLG00000036127
rosa_multiflora	Rmu_sc0007961.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010830
rosa_rugosa	Rorug05G0405200
rosa_samantha	Rh5AG460800 Rh5BG479100 Rh5CG503300 Rh5DG491000
rosa_wichuraiana	Rw5G042950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1103
Acc36I	ACCTGC	3 cut(s)	1672, 2215, 2260
Acc65I	GGTACC	3 cut(s)	293, 1627, 2353
AccB1I	GGYRCC	3 cut(s)	293, 1627, 2353
AccII	CGCG	3 cut(s)	1137, 2340, 2424
AccIII	TCCGGA	3 cut(s)	158, 1589, 1918
AciI	CCGC	3 cut(s)	2357, 2405, 2467
AcoI	YGGCCR	4 cut(s)	1028, 2285, 2310, 2464
AcsI	RAATTY	1 cut(s)	33
AcuI	CTGAAG	5 cut(s)	33, 620, 1142, 1914, 2201
AdeI	CACNNNGTG	1 cut(s)	658
AfaI	GTAC	6 cut(s)	295, 516, 1481, 1607, 1629, 2355
AfiI	CCNNNNNNNGG	4 cut(s)	1433, 1571, 2177, 2283
AflII	CTTAAG	1 cut(s)	563
AflIII	ACRYGT	4 cut(s)	774, 911, 1135, 1251
AhdI	GACNNNNNGTC	1 cut(s)	1649
AjnI	CCWGG	4 cut(s)	1071, 2234, 2319, 2387
AluBI	AGCT	9 cut(s)	422, 562, 824, 1019, 1088, 1223, 1634, 2336, 2371
AluI	AGCT	9 cut(s)	422, 562, 824, 1019, 1088, 1223, 1634, 2336, 2371
Alw21I	GWGCWC	2 cut(s)	78, 2449
Alw26I	GTCTC	4 cut(s)	108, 1461, 1540, 1591
AlwNI	CAGNNNCTG	2 cut(s)	1019, 1412
Aor13HI	TCCGGA	3 cut(s)	158, 1589, 1918
AoxI	GGCC	8 cut(s)	874, 1028, 1562, 1756, 2199, 2285, 2310, 2464
ApeKI	GCWGC	5 cut(s)	419, 466, 956, 2303, 2368
ApoI	RAATTY	1 cut(s)	33
ArsI	GACNNNNNNTTYG	2 cut(s)	2222, 2254
Asp718I	GGTACC	3 cut(s)	293, 1627, 2353
AspLEI	GCGC	2 cut(s)	1120, 2146
AspS9I	GGNCC	7 cut(s)	489, 874, 1562, 1757, 2181, 2200, 2238
AsuC2I	CCSGG	4 cut(s)	1445, 1910, 2179, 2198
AsuHPI	GGTGA	4 cut(s)	296, 518, 1333, 1630
AvaII	GGWCC	3 cut(s)	489, 2181, 2238
BaeGI	GKGCMC	1 cut(s)	2271
BaeI	ACNNNNGTAYC	4 cut(s)	1611, 1644, 2345, 2378
BamHI	GGATCC	1 cut(s)	1440
BanI	GGYRCC	3 cut(s)	293, 1627, 2353
BbsI	GAAGAC	4 cut(s)	164, 987, 1456, 1884
Bbv12I	GWGCWC	2 cut(s)	78, 2449
BbvCI	CCTCAGC	1 cut(s)	119
BbvI	GCAGC	5 cut(s)	431, 453, 943, 2290, 2380
BccI	CCATC	5 cut(s)	503, 804, 1306, 1431, 2198
BceAI	ACGGC	1 cut(s)	2272
BcgI	CGANNNNNNTGC	2 cut(s)	1536, 1570
BciT130I	CCWGG	4 cut(s)	1073, 2236, 2321, 2389
BciVI	GTATCC	1 cut(s)	1237
BclI	TGATCA	1 cut(s)	1740
BcnI	CCSGG	4 cut(s)	1445, 1910, 2179, 2198
BcoDI	GTCTC	4 cut(s)	108, 1461, 1540, 1591
BfaI	CTAG	4 cut(s)	1047, 1566, 2162, 2172
BfmI	CTRYAG	3 cut(s)	1985, 2082, 2366
BfrI	CTTAAG	1 cut(s)	563
BfuAI	ACCTGC	3 cut(s)	1672, 2215, 2260
BfuI	GTATCC	1 cut(s)	1237
BisI	GCNGC	7 cut(s)	420, 467, 957, 2304, 2369, 2405, 2467
BlsI	GCNGC	7 cut(s)	421, 468, 958, 2305, 2370, 2406, 2468
Bme1390I	CCNGG	8 cut(s)	1073, 1445, 1910, 2179, 2198, 2236, 2321, 2389
Bme18I	GGWCC	3 cut(s)	489, 2181, 2238
BmeRI	GACNNNNNGTC	1 cut(s)	1649
BmgT120I	GGNCC	7 cut(s)	489, 874, 1562, 1757, 2181, 2200, 2238
BmiI	GGNNCC	7 cut(s)	295, 490, 1442, 1629, 2182, 2325, 2355
BmrFI	CCNGG	8 cut(s)	1073, 1445, 1910, 2179, 2198, 2236, 2321, 2389
BmrI	ACTGGG	1 cut(s)	1632
BmsI	GCATC	7 cut(s)	649, 810, 1064, 1418, 1879, 2069, 2264
BmuI	ACTGGG	1 cut(s)	1632
BpiI	GAAGAC	4 cut(s)	164, 987, 1456, 1884
BpmI	CTGGAG	2 cut(s)	750, 2031
Bpu10I	CCTNAGC	2 cut(s)	119, 1558
BpuMI	CCSGG	4 cut(s)	1445, 1910, 2179, 2198
BsaAI	YACGTR	1 cut(s)	1964
BsaBI	GATNNNNATC	2 cut(s)	814, 1920
BsaI	GGTCTC	2 cut(s)	108, 1540
BsaJI	CCNNGG	4 cut(s)	41, 1908, 1932, 2461
BsaWI	WCCGGW	3 cut(s)	158, 1589, 1918
Bsc4I	CCNNNNNNNGG	4 cut(s)	1433, 1571, 2177, 2283
Bse118I	RCCGGY	2 cut(s)	1030, 2308
Bse1I	ACTGG	5 cut(s)	27, 767, 1108, 1627, 2264
Bse3DI	GCAATG	4 cut(s)	234, 864, 2298, 2412
Bse8I	GATNNNNATC	2 cut(s)	814, 1920
BseAI	TCCGGA	3 cut(s)	158, 1589, 1918
BseBI	CCWGG	4 cut(s)	1073, 2236, 2321, 2389
BseDI	CCNNGG	4 cut(s)	41, 1908, 1932, 2461
BseGI	GGATG	9 cut(s)	815, 984, 1206, 1442, 1918, 2060, 2190, 2255, 2384
BseJI	GATNNNNATC	2 cut(s)	814, 1920
BseLI	CCNNNNNNNGG	4 cut(s)	1433, 1571, 2177, 2283
BseMI	GCAATG	4 cut(s)	234, 864, 2298, 2412
BseMII	CTCAG	1 cut(s)	133
BseNI	ACTGG	5 cut(s)	27, 767, 1108, 1627, 2264
BseRI	GAGGAG	1 cut(s)	305
BseSI	GKGCMC	1 cut(s)	2271
BseX3I	CGGCCG	1 cut(s)	2464
BseXI	GCAGC	5 cut(s)	431, 453, 943, 2290, 2380
BseYI	CCCAGC	1 cut(s)	2259
BsgI	GTGCAG	3 cut(s)	438, 1880, 2289
Bsh1236I	CGCG	3 cut(s)	1137, 2340, 2424
Bsh1285I	CGRYCG	1 cut(s)	2467
BshFI	GGCC	8 cut(s)	876, 1030, 1564, 1758, 2201, 2287, 2312, 2466
BshNI	GGYRCC	3 cut(s)	293, 1627, 2353
BsiEI	CGRYCG	1 cut(s)	2467
BsiHKAI	GWGCWC	2 cut(s)	78, 2449
BsiSI	CCGG	9 cut(s)	159, 1031, 1445, 1590, 1910, 1919, 2178, 2198, 2309
BslFI	GGGAC	2 cut(s)	502, 1510
BslI	CCNNNNNNNGG	4 cut(s)	1433, 1571, 2177, 2283
BsmAI	GTCTC	4 cut(s)	108, 1461, 1540, 1591
BsmFI	GGGAC	2 cut(s)	502, 1510
BsmI	GAATGC	3 cut(s)	1667, 2032, 2146
BsnI	GGCC	8 cut(s)	876, 1030, 1564, 1758, 2201, 2287, 2312, 2466
Bso31I	GGTCTC	2 cut(s)	108, 1540
Bsp1286I	GDGCHC	3 cut(s)	78, 2271, 2449
Bsp13I	TCCGGA	3 cut(s)	158, 1589, 1918
Bsp1407I	TGTACA	2 cut(s)	1479, 1605
Bsp19I	CCATGG	1 cut(s)	1932
Bsp68I	TCGCGA	1 cut(s)	2340
BspACI	CCGC	3 cut(s)	2357, 2405, 2467
BspANI	GGCC	8 cut(s)	876, 1030, 1564, 1758, 2201, 2287, 2312, 2466
BspCNI	CTCAG	1 cut(s)	132
BspEI	TCCGGA	3 cut(s)	158, 1589, 1918
BspFNI	CGCG	3 cut(s)	1137, 2340, 2424
BspHI	TCATGA	1 cut(s)	175
BspLI	GGNNCC	7 cut(s)	295, 490, 1442, 1629, 2182, 2325, 2355
BspMAI	CTGCAG	2 cut(s)	1989, 2370
BspMI	ACCTGC	3 cut(s)	1672, 2215, 2260
BspT107I	GGYRCC	3 cut(s)	293, 1627, 2353
BspTI	CTTAAG	1 cut(s)	563
BspTNI	GGTCTC	2 cut(s)	108, 1540
BsrDI	GCAATG	4 cut(s)	234, 864, 2298, 2412
BsrFI	RCCGGY	2 cut(s)	1030, 2308
BsrGI	TGTACA	2 cut(s)	1479, 1605
BsrI	ACTGG	5 cut(s)	27, 767, 1108, 1627, 2264
BssAI	RCCGGY	2 cut(s)	1030, 2308
BssECI	CCNNGG	4 cut(s)	41, 1908, 1932, 2461
BssT1I	CCWWGG	2 cut(s)	41, 1932
Bst2UI	CCWGG	4 cut(s)	1073, 2236, 2321, 2389
Bst4CI	ACNGT	8 cut(s)	73, 375, 688, 1288, 1826, 1883, 2435, 2451
Bst6I	CTCTTC	4 cut(s)	183, 248, 889, 2191
BstAFI	CTTAAG	1 cut(s)	563
BstAPI	GCANNNNNTGC	1 cut(s)	863
BstAUI	TGTACA	2 cut(s)	1479, 1605
BstBAI	YACGTR	1 cut(s)	1964
BstC8I	GCNNGC	2 cut(s)	2338, 2366
BstDEI	CTNAG	3 cut(s)	119, 788, 1558
BstDSI	CCRYGG	2 cut(s)	1932, 2461
BstF5I	GGATG	9 cut(s)	815, 984, 1206, 1442, 1918, 2060, 2190, 2255, 2384
BstFNI	CGCG	3 cut(s)	1137, 2340, 2424
BstHHI	GCGC	2 cut(s)	1120, 2146
BstMAI	GTCTC	4 cut(s)	108, 1461, 1540, 1591
BstMCI	CGRYCG	1 cut(s)	2467
BstMWI	GCNNNNNNNGC	9 cut(s)	622, 708, 863, 1052, 1134, 1904, 2207, 2309, 2365
BstNI	CCWGG	4 cut(s)	1073, 2236, 2321, 2389
BstNSI	RCATGY	5 cut(s)	778, 915, 1255, 1777, 2042
BstSCI	CCNGG	8 cut(s)	1071, 1443, 1908, 2177, 2196, 2234, 2319, 2387
BstSFI	CTRYAG	3 cut(s)	1985, 2082, 2366
BstSLI	GKGCMC	1 cut(s)	2271
BstUI	CGCG	3 cut(s)	1137, 2340, 2424
BstV1I	GCAGC	5 cut(s)	431, 453, 943, 2290, 2380
BstV2I	GAAGAC	4 cut(s)	164, 987, 1456, 1884
BstX2I	RGATCY	7 cut(s)	1422, 1440, 1700, 1807, 2167, 2174, 2391
BstXI	CCANNNNNNTGG	1 cut(s)	1724
BstYI	RGATCY	7 cut(s)	1422, 1440, 1700, 1807, 2167, 2174, 2391
BstZI	CGGCCG	1 cut(s)	2464
BsuI	GTATCC	1 cut(s)	1237
BsuRI	GGCC	8 cut(s)	876, 1030, 1564, 1758, 2201, 2287, 2312, 2466
BtgI	CCRYGG	2 cut(s)	1932, 2461
BtsCI	GGATG	9 cut(s)	815, 984, 1206, 1442, 1918, 2060, 2190, 2255, 2384
BtsI	GCAGTG	3 cut(s)	552, 1284, 1982
BtsIMutI	CAGTG	7 cut(s)	552, 740, 1115, 1284, 1284, 1982, 2271
BtuMI	TCGCGA	1 cut(s)	2340
BveI	ACCTGC	3 cut(s)	1672, 2215, 2260
Cac8I	GCNNGC	2 cut(s)	2338, 2366
CaiI	CAGNNNCTG	2 cut(s)	1019, 1412
CciI	TCATGA	1 cut(s)	175
CfoI	GCGC	2 cut(s)	1120, 2146
Cfr10I	RCCGGY	2 cut(s)	1030, 2308
Cfr13I	GGNCC	7 cut(s)	489, 874, 1562, 1757, 2181, 2200, 2238
CseI	GACGC	1 cut(s)	1126
Csp6I	GTAC	6 cut(s)	294, 515, 1480, 1606, 1628, 2354
CviQI	GTAC	6 cut(s)	294, 515, 1480, 1606, 1628, 2354
DdeI	CTNAG	3 cut(s)	119, 788, 1558
DraIII	CACNNNGTG	1 cut(s)	658
DriI	GACNNNNNGTC	1 cut(s)	1649
EaeI	YGGCCR	4 cut(s)	1028, 2285, 2310, 2464
EagI	CGGCCG	1 cut(s)	2464
Eam1104I	CTCTTC	4 cut(s)	183, 248, 889, 2191
Eam1105I	GACNNNNNGTC	1 cut(s)	1649
EarI	CTCTTC	4 cut(s)	183, 248, 889, 2191
EclXI	CGGCCG	1 cut(s)	2464
Eco130I	CCWWGG	2 cut(s)	41, 1932
Eco31I	GGTCTC	2 cut(s)	108, 1540
Eco47I	GGWCC	3 cut(s)	489, 2181, 2238
Eco52I	CGGCCG	1 cut(s)	2464
Eco57I	CTGAAG	5 cut(s)	33, 620, 1142, 1914, 2201
EcoO109I	RGGNCCY	3 cut(s)	489, 1562, 2181
EcoRII	CCWGG	4 cut(s)	1071, 2234, 2319, 2387
EcoT14I	CCWWGG	2 cut(s)	41, 1932
EcoT22I	ATGCAT	1 cut(s)	1267
ErhI	CCWWGG	2 cut(s)	41, 1932
FalI	AAGNNNNNCTT	4 cut(s)	137, 169, 890, 922
FaqI	GGGAC	2 cut(s)	502, 1510
FauNDI	CATATG	1 cut(s)	1267
FbaI	TGATCA	1 cut(s)	1740
Fnu4HI	GCNGC	7 cut(s)	420, 467, 957, 2304, 2369, 2405, 2467
FokI	GGATG	9 cut(s)	822, 971, 1213, 1449, 1925, 2047, 2177, 2242, 2371
Fsp4HI	GCNGC	7 cut(s)	420, 467, 957, 2304, 2369, 2405, 2467
FspBI	CTAG	4 cut(s)	1047, 1566, 2162, 2172
GlaI	GCGC	2 cut(s)	1119, 2145
GluI	GCNGC	7 cut(s)	420, 467, 957, 2304, 2369, 2405, 2467
GsaI	CCCAGC	1 cut(s)	2263
GsuI	CTGGAG	2 cut(s)	750, 2031
HaeIII	GGCC	8 cut(s)	876, 1030, 1564, 1758, 2201, 2287, 2312, 2466
HapII	CCGG	9 cut(s)	159, 1031, 1445, 1590, 1910, 1919, 2178, 2198, 2309
HgaI	GACGC	1 cut(s)	1126
HhaI	GCGC	2 cut(s)	1120, 2146
Hin6I	GCGC	2 cut(s)	1118, 2144
HinP1I	GCGC	2 cut(s)	1118, 2144
HindIII	AAGCTT	1 cut(s)	560
HinfI	GANTC	7 cut(s)	102, 169, 739, 1979, 2006, 2381, 2438
HpaII	CCGG	9 cut(s)	159, 1031, 1445, 1590, 1910, 1919, 2178, 2198, 2309
HphI	GGTGA	4 cut(s)	296, 518, 1333, 1630
Hpy166II	GTNNAC	8 cut(s)	142, 515, 570, 772, 1482, 1606, 1608, 2295
Hpy188I	TCNGA	6 cut(s)	13, 379, 426, 1643, 1700, 1926
Hpy8I	GTNNAC	8 cut(s)	142, 515, 570, 772, 1482, 1606, 1608, 2295
Hpy99I	CGWCG	1 cut(s)	2429
HpyCH4III	ACNGT	8 cut(s)	73, 375, 688, 1288, 1826, 1883, 2435, 2451
HpyCH4IV	ACGT	1 cut(s)	1963
HpyF10VI	GCNNNNNNNGC	9 cut(s)	622, 708, 863, 1052, 1134, 1904, 2207, 2309, 2365
HpyF3I	CTNAG	3 cut(s)	119, 788, 1558
HpySE526I	ACGT	1 cut(s)	1963
HspAI	GCGC	2 cut(s)	1118, 2144
Kpn2I	TCCGGA	3 cut(s)	158, 1589, 1918
KpnI	GGTACC	3 cut(s)	297, 1631, 2357
Ksp22I	TGATCA	1 cut(s)	1740
LmnI	GCTCC	2 cut(s)	679, 2376
Lsp1109I	GCAGC	5 cut(s)	431, 453, 943, 2290, 2380
LweI	GCATC	7 cut(s)	649, 810, 1064, 1418, 1879, 2069, 2264
MaeI	CTAG	4 cut(s)	1047, 1566, 2162, 2172
MaeII	ACGT	1 cut(s)	1963
MfeI	CAATTG	4 cut(s)	501, 1195, 1230, 1386
MflI	RGATCY	7 cut(s)	1422, 1440, 1700, 1807, 2167, 2174, 2391
MhlI	GDGCHC	3 cut(s)	78, 2271, 2449
MluI	ACGCGT	1 cut(s)	1135
MlyI	GAGTC	4 cut(s)	111, 178, 1988, 2375
Mph1103I	ATGCAT	1 cut(s)	1267
MroI	TCCGGA	3 cut(s)	158, 1589, 1918
MseI	TTAA	5 cut(s)	335, 564, 1205, 1370, 1542
MslI	CAYNNNNRTG	5 cut(s)	263, 1266, 1289, 1722, 2037
MspA1I	CMGCKG	2 cut(s)	1634, 2359
MspCI	CTTAAG	1 cut(s)	563
MspI	CCGG	9 cut(s)	159, 1031, 1445, 1590, 1910, 1919, 2178, 2198, 2309
MspR9I	CCNGG	8 cut(s)	1073, 1445, 1910, 2179, 2198, 2236, 2321, 2389
MunI	CAATTG	4 cut(s)	501, 1195, 1230, 1386
Mva1269I	GAATGC	3 cut(s)	1667, 2032, 2146
MvaI	CCWGG	4 cut(s)	1073, 2236, 2321, 2389
MvnI	CGCG	3 cut(s)	1137, 2340, 2424
MwoI	GCNNNNNNNGC	9 cut(s)	622, 708, 863, 1052, 1134, 1904, 2207, 2309, 2365
NciI	CCSGG	4 cut(s)	1445, 1910, 2179, 2198
NcoI	CCATGG	1 cut(s)	1932
NdeI	CATATG	1 cut(s)	1267
NlaIV	GGNNCC	7 cut(s)	295, 490, 1442, 1629, 2182, 2325, 2355
NruI	TCGCGA	1 cut(s)	2340
NsiI	ATGCAT	1 cut(s)	1267
NspI	RCATGY	5 cut(s)	778, 915, 1255, 1777, 2042
PagI	TCATGA	1 cut(s)	175
PciI	ACATGT	3 cut(s)	774, 911, 1251
PctI	GAATGC	3 cut(s)	1667, 2032, 2146
PfeI	GAWTC	3 cut(s)	739, 2006, 2438
PfoI	TCCNGGA	1 cut(s)	2387
PkrI	GCNGC	7 cut(s)	421, 468, 958, 2305, 2370, 2406, 2468
PleI	GAGTC	4 cut(s)	110, 177, 1987, 2375
PpsI	GAGTC	4 cut(s)	110, 177, 1987, 2375
Ppu21I	YACGTR	1 cut(s)	1964
PpuMI	RGGWCCY	2 cut(s)	489, 2181
PscI	ACATGT	3 cut(s)	774, 911, 1251
PsiI	TTATAA	1 cut(s)	1103
Psp5II	RGGWCCY	2 cut(s)	489, 2181
Psp6I	CCWGG	4 cut(s)	1071, 2234, 2319, 2387
PspFI	CCCAGC	1 cut(s)	2259
PspGI	CCWGG	4 cut(s)	1071, 2234, 2319, 2387
PspN4I	GGNNCC	7 cut(s)	295, 490, 1442, 1629, 2182, 2325, 2355
PspPI	GGNCC	7 cut(s)	489, 874, 1562, 1757, 2181, 2200, 2238
PspPPI	RGGWCCY	2 cut(s)	489, 2181
PstI	CTGCAG	2 cut(s)	1989, 2370
PstNI	CAGNNNCTG	2 cut(s)	1019, 1412
PsuI	RGATCY	7 cut(s)	1422, 1440, 1700, 1807, 2167, 2174, 2391
PvuII	CAGCTG	1 cut(s)	1634
RruI	TCGCGA	1 cut(s)	2340
RsaI	GTAC	6 cut(s)	295, 516, 1481, 1607, 1629, 2355
RsaNI	GTAC	6 cut(s)	294, 515, 1480, 1606, 1628, 2354
RseI	CAYNNNNRTG	5 cut(s)	263, 1266, 1289, 1722, 2037
SaqAI	TTAA	5 cut(s)	335, 564, 1205, 1370, 1542
SatI	GCNGC	7 cut(s)	420, 467, 957, 2304, 2369, 2405, 2467
Sau96I	GGNCC	7 cut(s)	489, 874, 1562, 1757, 2181, 2200, 2238
SchI	GAGTC	4 cut(s)	111, 178, 1988, 2375
ScrFI	CCNGG	8 cut(s)	1073, 1445, 1910, 2179, 2198, 2236, 2321, 2389
SduI	GDGCHC	3 cut(s)	78, 2271, 2449
SfaNI	GCATC	7 cut(s)	649, 810, 1064, 1418, 1879, 2069, 2264
SfcI	CTRYAG	3 cut(s)	1985, 2082, 2366
SinI	GGWCC	3 cut(s)	489, 2181, 2238
SmiMI	CAYNNNNRTG	5 cut(s)	263, 1266, 1289, 1722, 2037
SmlI	CTYRAG	1 cut(s)	563
SmoI	CTYRAG	1 cut(s)	563
SsiI	CCGC	3 cut(s)	2357, 2405, 2467
SspMI	CTAG	4 cut(s)	1047, 1566, 2162, 2172
StyD4I	CCNGG	8 cut(s)	1071, 1443, 1908, 2177, 2196, 2234, 2319, 2387
StyI	CCWWGG	2 cut(s)	41, 1932
TaaI	ACNGT	8 cut(s)	73, 375, 688, 1288, 1826, 1883, 2435, 2451
TaiI	ACGT	1 cut(s)	1966
TaqI	TCGA	6 cut(s)	438, 804, 1157, 1833, 2379, 2441
TatI	WGTACW	3 cut(s)	514, 1479, 1605
TauI	GCSGC	2 cut(s)	2407, 2469
TfiI	GAWTC	3 cut(s)	739, 2006, 2438
Tru1I	TTAA	5 cut(s)	335, 564, 1205, 1370, 1542
Tru9I	TTAA	5 cut(s)	335, 564, 1205, 1370, 1542
TscAI	CASTG	7 cut(s)	552, 740, 1115, 1284, 1291, 1989, 2271
TseI	GCWGC	5 cut(s)	419, 466, 956, 2303, 2368
TspGWI	ACGGA	3 cut(s)	513, 660, 1693
TspRI	CASTG	7 cut(s)	552, 740, 1115, 1284, 1291, 1989, 2271
Vha464I	CTTAAG	1 cut(s)	563
VpaK11BI	GGWCC	3 cut(s)	489, 2181, 2238
XapI	RAATTY	1 cut(s)	33
XceI	RCATGY	5 cut(s)	778, 915, 1255, 1777, 2042
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1194
XspI	CTAG	4 cut(s)	1047, 1566, 2162, 2172
Zsp2I	ATGCAT	1 cut(s)	1267

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.