RLG00000036486

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0021541
Physical Location & Seq
Forward (+)
81210131 .. 81211375
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036486

Sequence Viewer

Length: 654 bp
ATGGCTGACCCGCTGTCATCACAGGACAAGGGACACCACCACCAGAGCCACGAATGGAACGTGAAGCCCAACTTTGCACCACCATCCCTTGAACCTATCCTCGATCTCACCTGCCTCAGAGATGCATCAAGTCAGGAGACCCAGGCAGTAGTCCACTTTCATCAGTCTTCATACCAGAATTCAACCTGCAGCCAAGAGCGAGCTTTAGAAAAACACAGCTCGTCCGGCAGCAGCACCACCAAGTCGCAAGACCAGGGCAGAAACAAACGTTGGGCCGCTGTCGGACGAGCACCGCTGGATTGCGTTGGAAATCCACCTAGGCGACCACCGTCAGCCTCGAAAATCGGCGTCCTTGAGTCTGGTTCAATTAAAGAGCAAAAGCAAGCGGCGATGGAGATCAGGCTCCTCGCCAAAAACAAATCAAAAACTGGTTCGATCTCCGTCACTGTGCTCGGCGTGAACCGAATTTGGAATTCTGGATCGGAAATTTTCGGTCATCAGTTCTTTGCTCGGCGTCGCTGTGCTCGGCGTGTCGTCCTAACTCGGTTCAGCAACTCGGCCAAGTCCAAGTCGAATTGGATTTTGTTAGCTAGAAAGTCGCCGCTTTCTGTGCTCAGCTTTGTTGTCACTGACTCTGTCTGGAGCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

218

Amino Acids

24.04

Weight (kDa)

10.22

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021541)

Species Orthologous Gene IDs
rosa_laevigata RLG00000011211 RLG00000036486 RLG00000036670
rosa_multiflora Rmu_sc0000407.1_g000002
rosa_roxburghii Rroxscaffold_7G00166290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 2 cut(s) 119, 194
AciI CCGC 5 cut(s) 11, 276, 293, 386, 602
AclI AACGTT 1 cut(s) 268
AclWI GGATC 1 cut(s) 487
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 4 cut(s) 178, 465, 472, 486
AcyI GRCGYC 2 cut(s) 348, 514
AgsI TTSAA 3 cut(s) 92, 183, 366
AhdI GACNNNNNGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 141, 252
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 4 cut(s) 203, 219, 590, 618
AluI AGCT 4 cut(s) 203, 219, 590, 618
Alw21I GWGCWC 4 cut(s) 292, 453, 526, 615
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 487
AoxI GGCC 2 cut(s) 273, 558
ApeKI GCWGC 3 cut(s) 189, 228, 231
ApoI RAATTY 4 cut(s) 178, 465, 472, 486
AspA2I CCTAGG 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 100
AvrII CCTAGG 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 159
Bbv12I GWGCWC 4 cut(s) 292, 453, 526, 615
BbvI GCAGC 3 cut(s) 201, 240, 243
BccI CCATC 2 cut(s) 91, 385
BciT130I CCWGG 2 cut(s) 143, 254
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 318, 591
BfmI CTRYAG 1 cut(s) 187
BfuAI ACCTGC 2 cut(s) 119, 194
BisI GCNGC 6 cut(s) 190, 229, 232, 276, 387, 602
BlnI CCTAGG 1 cut(s) 317
BlpI GCTNAGC 1 cut(s) 614
BlsI GCNGC 6 cut(s) 191, 230, 233, 277, 388, 603
Bme1390I CCNGG 2 cut(s) 143, 254
BmeRI GACNNNNNGTC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 2 cut(s) 143, 254
BmsI GCATC 2 cut(s) 112, 134
BoxI GACNNNNGTC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 159
Bpu1102I GCTNAGC 1 cut(s) 614
BpuEI CTTGAG 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 395
BsaHI GRCGYC 2 cut(s) 348, 514
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 141, 253, 317
Bse1I ACTGG 1 cut(s) 433
Bse8I GATNNNNATC 1 cut(s) 395
BseBI CCWGG 2 cut(s) 143, 254
BseDI CCNNGG 3 cut(s) 141, 253, 317
BseGI GGATG 1 cut(s) 83
BseJI GATNNNNATC 1 cut(s) 395
BseMII CTCAG 2 cut(s) 130, 628
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 3 cut(s) 201, 240, 243
BshFI GGCC 2 cut(s) 275, 560
BsiHKAI GWGCWC 4 cut(s) 292, 453, 526, 615
BsiSI CCGG 1 cut(s) 225
BslFI GGGAC 1 cut(s) 45
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 2 cut(s) 275, 560
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 4 cut(s) 292, 453, 526, 615
Bsp143I GATC 4 cut(s) 103, 396, 435, 479
Bsp1720I GCTNAGC 1 cut(s) 614
BspACI CCGC 5 cut(s) 11, 276, 293, 386, 602
BspANI GGCC 2 cut(s) 275, 560
BspCNI CTCAG 2 cut(s) 129, 627
BspLI GGNNCC 1 cut(s) 404
BspMAI CTGCAG 1 cut(s) 191
BspMI ACCTGC 2 cut(s) 119, 194
BspPI GGATC 1 cut(s) 487
BspTNI GGTCTC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 3 cut(s) 141, 253, 317
BssMI GATC 4 cut(s) 103, 396, 435, 479
BssNI GRCGYC 2 cut(s) 348, 514
BssT1I CCWWGG 1 cut(s) 317
Bst2UI CCWGG 2 cut(s) 143, 254
Bst4CI ACNGT 2 cut(s) 330, 448
BstACI GRCGYC 2 cut(s) 348, 514
BstC8I GCNNGC 2 cut(s) 201, 384
BstDEI CTNAG 2 cut(s) 116, 614
BstF5I GGATG 1 cut(s) 83
BstKTI GATC 4 cut(s) 106, 399, 438, 482
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 4 cut(s) 103, 396, 435, 479
BstMWI GCNNNNNNNGC 2 cut(s) 225, 610
BstNI CCWGG 2 cut(s) 143, 254
BstPAI GACNNNNGTC 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 141, 252
BstSFI CTRYAG 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 201, 240, 243
BstV2I GAAGAC 1 cut(s) 159
BsuRI GGCC 2 cut(s) 275, 560
BtgZI GCGATG 1 cut(s) 404
BtsCI GGATG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 444, 627
BveI ACCTGC 2 cut(s) 119, 194
Cac8I GCNNGC 2 cut(s) 201, 384
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 2 cut(s) 337, 503
DdeI CTNAG 2 cut(s) 116, 614
DpnI GATC 4 cut(s) 105, 398, 437, 481
DpnII GATC 4 cut(s) 103, 396, 435, 479
DriI GACNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 558
Eam1105I GACNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 131
EcoRI GAATTC 2 cut(s) 178, 472
EcoRII CCWGG 2 cut(s) 141, 252
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 317
FaiI YATR 1 cut(s) 172
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 45
FauI CCCGC 1 cut(s) 18
Fnu4HI GCNGC 6 cut(s) 190, 229, 232, 276, 387, 602
FokI GGATG 1 cut(s) 70
Fsp4HI GCNGC 6 cut(s) 190, 229, 232, 276, 387, 602
FspBI CTAG 2 cut(s) 318, 591
GluI GCNGC 6 cut(s) 190, 229, 232, 276, 387, 602
HaeIII GGCC 2 cut(s) 275, 560
HapII CCGG 1 cut(s) 225
HgaI GACGC 2 cut(s) 337, 503
Hin1I GRCGYC 2 cut(s) 348, 514
HinfI GANTC 2 cut(s) 356, 632
HpaII CCGG 1 cut(s) 225
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 154, 460
Hpy188I TCNGA 4 cut(s) 119, 284, 484, 653
Hpy188III TCNNGA 3 cut(s) 134, 477, 640
Hpy8I GTNNAC 2 cut(s) 154, 460
Hpy99I CGWCG 1 cut(s) 519
HpyCH4III ACNGT 2 cut(s) 330, 448
HpyCH4IV ACGT 2 cut(s) 60, 268
HpyCH4V TGCA 3 cut(s) 77, 125, 189
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 610
HpyF3I CTNAG 2 cut(s) 116, 614
HpySE526I ACGT 2 cut(s) 60, 268
Hsp92I GRCGYC 2 cut(s) 348, 514
Kzo9I GATC 4 cut(s) 103, 396, 435, 479
LmnI GCTCC 2 cut(s) 408, 642
Lsp1109I GCAGC 3 cut(s) 201, 240, 243
LweI GCATC 2 cut(s) 112, 134
MaeI CTAG 2 cut(s) 318, 591
MaeII ACGT 2 cut(s) 60, 268
MaeIII GTNAC 2 cut(s) 442, 625
MalI GATC 4 cut(s) 105, 398, 437, 481
MboI GATC 4 cut(s) 103, 396, 435, 479
MboII GAAGA 1 cut(s) 159
MhlI GDGCHC 4 cut(s) 292, 453, 526, 615
MluCI AATT 6 cut(s) 178, 366, 465, 472, 486, 574
MlyI GAGTC 2 cut(s) 365, 626
MmeI TCCRAC 2 cut(s) 262, 286
MnlI CCTC 4 cut(s) 110, 125, 346, 416
Mph1103I ATGCAT 1 cut(s) 127
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 3 cut(s) 13, 278, 295
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 2 cut(s) 143, 254
MvaI CCWGG 2 cut(s) 143, 254
MwoI GCNNNNNNNGC 2 cut(s) 225, 610
NdeII GATC 4 cut(s) 103, 396, 435, 479
NlaIV GGNNCC 1 cut(s) 404
NmeAIII GCCGAG 4 cut(s) 432, 490, 505, 536
NmuCI GTSAC 2 cut(s) 442, 625
NsiI ATGCAT 1 cut(s) 127
PaqCI CACCTGC 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 635
PkrI GCNGC 6 cut(s) 191, 230, 233, 277, 388, 603
PleI GAGTC 2 cut(s) 364, 626
PpsI GAGTC 2 cut(s) 364, 626
PshAI GACNNNNGTC 1 cut(s) 328
Psp1406I AACGTT 1 cut(s) 268
Psp6I CCWGG 2 cut(s) 141, 252
PspGI CCWGG 2 cut(s) 141, 252
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 1 cut(s) 273
PstI CTGCAG 1 cut(s) 191
PsyI GACNNNGTC 1 cut(s) 635
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 6 cut(s) 190, 229, 232, 276, 387, 602
Sau3AI GATC 4 cut(s) 103, 396, 435, 479
Sau96I GGNCC 1 cut(s) 273
SchI GAGTC 2 cut(s) 365, 626
ScrFI CCNGG 2 cut(s) 143, 254
SduI GDGCHC 4 cut(s) 292, 453, 526, 615
SfaNI GCATC 2 cut(s) 112, 134
SfcI CTRYAG 1 cut(s) 187
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 6 cut(s) 178, 366, 465, 472, 486, 574
SsiI CCGC 5 cut(s) 11, 276, 293, 386, 602
SspMI CTAG 2 cut(s) 318, 591
StyD4I CCNGG 2 cut(s) 141, 252
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 2 cut(s) 330, 448
TaiI ACGT 2 cut(s) 63, 271
TaqI TCGA 4 cut(s) 102, 338, 434, 572
TaqII GACCGA 1 cut(s) 482
TasI AATT 6 cut(s) 178, 366, 465, 472, 486, 574
TauI GCSGC 3 cut(s) 278, 389, 604
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 451, 634
TseFI GTSAC 2 cut(s) 442, 625
TseI GCWGC 3 cut(s) 189, 228, 231
Tsp45I GTSAC 2 cut(s) 442, 625
TspDTI ATGAA 2 cut(s) 149, 159
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 2 cut(s) 451, 634
Tth111I GACNNNGTC 1 cut(s) 635
XapI RAATTY 4 cut(s) 178, 465, 472, 486
XmaJI CCTAGG 1 cut(s) 317
XspI CTAG 2 cut(s) 318, 591
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.