RLG00000036557

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019423
Physical Location & Seq
Forward (+)
81903682 .. 81905861
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036557

Sequence Viewer

Length: 1356 bp
ATGGAGAAAGCAAGTGCATTCCTCCGCCATCCTCCTCCTTGTAAGGGAAAACTCATCACTATCCTTAGCATTGATGGAGGTGGAGTCAAAGGGATTATCCCCGGAACTATTCTCGCCGTCTTAGAGAAGAAGCTTCAGGAAAAGGAGAGCAATACAAATGCAAGACTGGCCGATTATTTCGATGTGATTGCAGGGACGAGCACCGGTGGCCTTGTGACTGCCATGCTCACTGCTCCTGATGAAAACAACCGTCCCTTGTTCAGTGCTGACCAGATTGTCCCTTTTTATCTTGAAAACTGTCATAAGATCTTTCCACCAAGCAAGTCTGCATTTCTAAGCAACATGGATGAATTGCCCTCTGGTTATAGTGATGACACGCTTCAACATTTGAAGCTAATGCCGAATTCAGGATTTTCTCGTAGTTGGTTAGAGTGGTTATGGAAATTAGTTGCTAAGCTTCTTCCTGGTTCAATTGGGAAGCTACTGGGGAGTCCAAAATATGCTGCCACTGCGTTACGGGATATAGTTGAAGGAAAATTAGGAGACATACGGTTGAATCAGACATTGACCAATGTTGTAATCCCGAGTTATGATATAAGTCTGCTCCAACCAGCTATCTTCTCAACTTACGAGTTGAAAAGGAACGAGGAGCAAGATCCGGCAAAACTCTCTGATATATGCATGGCAACTTCAGCAGCACCAACCTATTTTCCTGCATATAAATTTGAAACCAATACTAGAGAATTCCATGTCGTTGACGGCGGAATGGCTGCTAATAACCCGGGTTTGGTTGCTATAACCGAAGTGACAAAAGAAATCAAGAATAAAAATCCTGACTTTCGAAAAGATTGGGCAGCTGATCAGGGCAATCGGTTCCTACTTATATCATTGGGTACTGGTTCACCAAAGCAAAAGAGTACATACAACGCCGACGAAGCAGCCAACTGGGGTATTGGGGGATGGATGATTCATTTGGATCCATTTTCTTGTCCATTGCAAGAAGTATTCACTGAAGCAAGTAGTGACATGGTCGATATCCACCTCACTACCCTTTTCGAATCTATTTGGTCCAAGGGAACTTACCTTCGAATTCAGCATGATCAATTGCCTACAGACATGAATTCTATGGACAATGCTACAGAAGAAAACCTGAAAAATCTTGTGATAGCTGGGCAGGAGCTGTTACAGAAATCGGTTTCAAAGATGAATACAGCTACCGGTCGCCTTGAGACTGTTAAACCTGAGATCACAAATGAAAAGGCTCTCGAAATGATGGCGGAAAAACTCGTTGAGGAGAGGAAGAAACGTCAAAGCCGAGACCATGACCATGGTCACCAGAAGGAAATTGATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

452

Amino Acids

50.03

Weight (kDa)

5.68

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 265 5.4e-23 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019423)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0075361
rosa_laevigata RLG00000036557
rosa_multiflora Rmu_sc0000770.1_g000023
rosa_roxburghii Rroxscaffold_1G00006140
rosa_rugosa Rorug05G0439100
rosa_samantha Rh5AG495700 Rh5BG517300
rosa_wichuraiana Rw5G046000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AciI CCGC 3 cut(s) 25, 762, 1277
AclWI GGATC 3 cut(s) 650, 971, 984
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 5 cut(s) 403, 722, 743, 1089, 1120
AcuI CTGAAG 3 cut(s) 119, 675, 1032
AfaI GTAC 2 cut(s) 895, 919
AfiI CCNNNNNNNGG 3 cut(s) 44, 407, 787
AgeI ACCGGT 2 cut(s) 203, 1217
AgsI TTSAA 9 cut(s) 293, 383, 391, 471, 530, 556, 637, 728, 1200
AjnI CCWGG 1 cut(s) 463
AluBI AGCT 9 cut(s) 133, 394, 457, 481, 614, 857, 1169, 1180, 1214
AluI AGCT 9 cut(s) 133, 394, 457, 481, 614, 857, 1169, 1180, 1214
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 537, 1223, 1311
AlwI GGATC 3 cut(s) 650, 971, 984
AlwNI CAGNNNCTG 1 cut(s) 1180
Ama87I CYCGRG 2 cut(s) 583, 781
AoxI GGCC 2 cut(s) 168, 208
ApeKI GCWGC 5 cut(s) 503, 695, 770, 854, 938
ApoI RAATTY 5 cut(s) 403, 722, 743, 1089, 1120
AsiGI ACCGGT 2 cut(s) 203, 1217
AspS9I GGNCC 1 cut(s) 1068
AsuC2I CCSGG 3 cut(s) 102, 782, 783
AsuHPI GGTGA 2 cut(s) 894, 1325
AsuII TTCGAA 3 cut(s) 841, 1056, 1087
AvaI CYCGRG 2 cut(s) 583, 781
AvaII GGWCC 1 cut(s) 1068
BamHI GGATCC 1 cut(s) 976
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 5 cut(s) 490, 707, 757, 866, 950
BccI CCATC 4 cut(s) 36, 68, 954, 1267
BceAI ACGGC 2 cut(s) 101, 775
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 465
BclI TGATCA 2 cut(s) 859, 1099
BcnI CCSGG 3 cut(s) 102, 782, 783
BcoDI GTCTC 3 cut(s) 537, 1223, 1311
BfaI CTAG 2 cut(s) 738, 1354
BfmI CTRYAG 2 cut(s) 1110, 1137
BglII AGATCT 1 cut(s) 306
BisI GCNGC 5 cut(s) 504, 696, 771, 855, 939
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 5 cut(s) 505, 697, 772, 856, 940
Bme1390I CCNGG 4 cut(s) 102, 465, 782, 783
Bme18I GGWCC 1 cut(s) 1068
BmeT110I CYCGRG 2 cut(s) 583, 781
BmgT120I GGNCC 1 cut(s) 1068
BmiI GGNNCC 2 cut(s) 875, 978
BmrFI CCNGG 4 cut(s) 102, 465, 782, 783
BmrI ACTGGG 2 cut(s) 494, 955
BmsI GCATC 1 cut(s) 1339
BmuI ACTGGG 2 cut(s) 494, 955
BoxI GACNNNNGTC 1 cut(s) 1329
Bpu10I CCTNAGC 1 cut(s) 65
Bpu1102I GCTNAGC 1 cut(s) 453
Bpu14I TTCGAA 3 cut(s) 841, 1056, 1087
BpuEI CTTGAG 1 cut(s) 1247
BpuMI CCSGG 3 cut(s) 102, 782, 783
BsaI GGTCTC 1 cut(s) 1311
BsaJI CCNNGG 4 cut(s) 100, 781, 1071, 1327
BsaWI WCCGGW 2 cut(s) 203, 1217
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 407, 787
Bse118I RCCGGY 2 cut(s) 203, 1217
Bse1I ACTGG 4 cut(s) 171, 489, 901, 950
Bse3DI GCAATG 1 cut(s) 992
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 4 cut(s) 100, 781, 1071, 1327
BseGI GGATG 4 cut(s) 28, 352, 965, 969
BseLI CCNNNNNNNGG 3 cut(s) 44, 407, 787
BseMI GCAATG 1 cut(s) 992
BseMII CTCAG 1 cut(s) 1233
BseNI ACTGG 4 cut(s) 171, 489, 901, 950
BseRI GAGGAG 3 cut(s) 24, 662, 1307
BseXI GCAGC 5 cut(s) 490, 707, 757, 866, 950
BseYI CCCAGC 1 cut(s) 1169
Bsh1285I CGRYCG 1 cut(s) 1222
BshFI GGCC 2 cut(s) 170, 210
BshTI ACCGGT 2 cut(s) 203, 1217
BsiEI CGRYCG 1 cut(s) 1222
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 2 cut(s) 583, 781
BsiSI CCGG 5 cut(s) 102, 204, 659, 782, 1218
BslFI GGGAC 3 cut(s) 208, 237, 263
BslI CCNNNNNNNGG 3 cut(s) 44, 407, 787
BsmAI GTCTC 3 cut(s) 537, 1223, 1311
BsmFI GGGAC 3 cut(s) 208, 237, 263
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 170, 210
Bso31I GGTCTC 1 cut(s) 1311
BsoBI CYCGRG 2 cut(s) 583, 781
Bsp119I TTCGAA 3 cut(s) 841, 1056, 1087
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
Bsp1720I GCTNAGC 1 cut(s) 453
Bsp19I CCATGG 1 cut(s) 1327
BspACI CCGC 3 cut(s) 25, 762, 1277
BspANI GGCC 2 cut(s) 170, 210
BspCNI CTCAG 1 cut(s) 1234
BspLI GGNNCC 2 cut(s) 875, 978
BspPI GGATC 3 cut(s) 650, 971, 984
BspT104I TTCGAA 3 cut(s) 841, 1056, 1087
BspTNI GGTCTC 1 cut(s) 1311
BsrDI GCAATG 1 cut(s) 992
BsrFI RCCGGY 2 cut(s) 203, 1217
BsrI ACTGG 4 cut(s) 171, 489, 901, 950
BssAI RCCGGY 2 cut(s) 203, 1217
BssECI CCNNGG 4 cut(s) 100, 781, 1071, 1327
BssMI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
BssT1I CCWWGG 2 cut(s) 1071, 1327
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 4 cut(s) 251, 299, 552, 1234
BstBI TTCGAA 3 cut(s) 841, 1056, 1087
BstDEI CTNAG 5 cut(s) 65, 121, 335, 453, 1242
BstDSI CCRYGG 1 cut(s) 1327
BstEII GGTNACC 1 cut(s) 1331
BstF5I GGATG 4 cut(s) 28, 352, 965, 969
BstKTI GATC 6 cut(s) 309, 658, 862, 979, 1102, 1248
BstMAI GTCTC 3 cut(s) 537, 1223, 1311
BstMBI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
BstMCI CGRYCG 1 cut(s) 1222
BstMWI GCNNNNNNNGC 5 cut(s) 167, 207, 509, 692, 935
BstNI CCWGG 1 cut(s) 465
BstPAI GACNNNNGTC 1 cut(s) 1329
BstPI GGTNACC 1 cut(s) 1331
BstSCI CCNGG 4 cut(s) 100, 463, 780, 781
BstSFI CTRYAG 2 cut(s) 1110, 1137
BstV1I GCAGC 5 cut(s) 490, 707, 757, 866, 950
BstX2I RGATCY 3 cut(s) 306, 655, 976
BstXI CCANNNNNNTGG 1 cut(s) 1328
BstYI RGATCY 3 cut(s) 306, 655, 976
BsuRI GGCC 2 cut(s) 170, 210
BtgI CCRYGG 1 cut(s) 1327
BtsCI GGATG 4 cut(s) 28, 352, 965, 969
BtsI GCAGTG 2 cut(s) 228, 507
BtsIMutI CAGTG 4 cut(s) 228, 268, 507, 1008
CaiI CAGNNNCTG 1 cut(s) 1180
Cfr10I RCCGGY 2 cut(s) 203, 1217
Cfr13I GGNCC 1 cut(s) 1068
Cfr9I CCCGGG 1 cut(s) 781
Csp6I GTAC 2 cut(s) 894, 918
CspAI ACCGGT 2 cut(s) 203, 1217
CviAII CATG 9 cut(s) 223, 343, 682, 749, 1027, 1097, 1117, 1322, 1328
CviQI GTAC 2 cut(s) 894, 918
DdeI CTNAG 5 cut(s) 65, 121, 335, 453, 1242
DpnI GATC 6 cut(s) 308, 657, 861, 978, 1101, 1247
DpnII GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 168
EciI GGCGGA 3 cut(s) 14, 777, 1292
Eco130I CCWWGG 2 cut(s) 1071, 1327
Eco31I GGTCTC 1 cut(s) 1311
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 1 cut(s) 1068
Eco57I CTGAAG 3 cut(s) 119, 675, 1032
Eco88I CYCGRG 2 cut(s) 583, 781
Eco91I GGTNACC 1 cut(s) 1331
EcoO65I GGTNACC 1 cut(s) 1331
EcoRI GAATTC 4 cut(s) 403, 743, 1089, 1120
EcoRII CCWGG 1 cut(s) 463
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 2 cut(s) 1071, 1327
EcoT22I ATGCAT 1 cut(s) 683
ErhI CCWWGG 2 cut(s) 1071, 1327
FaeI CATG 9 cut(s) 226, 346, 685, 752, 1030, 1100, 1120, 1325, 1331
FaqI GGGAC 3 cut(s) 208, 237, 263
FatI CATG 9 cut(s) 222, 342, 681, 748, 1026, 1096, 1116, 1321, 1327
FbaI TGATCA 2 cut(s) 859, 1099
Fnu4HI GCNGC 5 cut(s) 504, 696, 771, 855, 939
FokI GGATG 4 cut(s) 15, 359, 972, 976
Fsp4HI GCNGC 5 cut(s) 504, 696, 771, 855, 939
FspBI CTAG 2 cut(s) 738, 1354
GluI GCNGC 5 cut(s) 504, 696, 771, 855, 939
GsaI CCCAGC 1 cut(s) 1173
HaeIII GGCC 2 cut(s) 170, 210
HapII CCGG 5 cut(s) 102, 204, 659, 782, 1218
Hin1II CATG 9 cut(s) 226, 346, 685, 752, 1030, 1100, 1120, 1325, 1331
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HindIII AAGCTT 2 cut(s) 131, 455
HinfI GANTC 5 cut(s) 84, 490, 556, 967, 1058
HpaII CCGG 5 cut(s) 102, 204, 659, 782, 1218
HphI GGTGA 2 cut(s) 894, 1325
Hpy166II GTNNAC 2 cut(s) 757, 902
Hpy188I TCNGA 2 cut(s) 561, 673
Hpy188III TCNNGA 8 cut(s) 137, 236, 290, 408, 583, 820, 833, 1265
Hpy8I GTNNAC 2 cut(s) 757, 902
Hpy99I CGWCG 1 cut(s) 935
HpyAV CCTTC 3 cut(s) 524, 1094, 1333
HpyCH4III ACNGT 4 cut(s) 251, 299, 552, 1234
HpyCH4IV ACGT 1 cut(s) 1306
HpyCH4V TGCA 7 cut(s) 17, 161, 191, 329, 681, 716, 997
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 207, 509, 692, 935
HpyF3I CTNAG 5 cut(s) 65, 121, 335, 453, 1242
HpySE526I ACGT 1 cut(s) 1306
Hsp92II CATG 9 cut(s) 226, 346, 685, 752, 1030, 1100, 1120, 1325, 1331
Ksp22I TGATCA 2 cut(s) 859, 1099
Kzo9I GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
LmnI GCTCC 4 cut(s) 238, 609, 649, 1177
Lsp1109I GCAGC 5 cut(s) 490, 707, 757, 866, 950
LweI GCATC 1 cut(s) 1339
MaeI CTAG 2 cut(s) 738, 1354
MaeII ACGT 1 cut(s) 1306
MaeIII GTNAC 6 cut(s) 214, 513, 805, 1022, 1182, 1331
MalI GATC 6 cut(s) 308, 657, 861, 978, 1101, 1247
MboI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
MboII GAAGA 5 cut(s) 139, 452, 610, 1154, 1312
MfeI CAATTG 2 cut(s) 471, 1103
MflI RGATCY 3 cut(s) 306, 655, 976
MhlI GDGCHC 1 cut(s) 203
MlyI GAGTC 2 cut(s) 93, 499
MmeI TCCRAC 1 cut(s) 631
MnlI CCTC 9 cut(s) 32, 42, 45, 71, 367, 640, 1052, 1285, 1290
Mph1103I ATGCAT 1 cut(s) 683
MseI TTAA 1 cut(s) 1236
MslI CAYNNNNRTG 1 cut(s) 1326
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 5 cut(s) 102, 204, 659, 782, 1218
MspR9I CCNGG 4 cut(s) 102, 465, 782, 783
MunI CAATTG 2 cut(s) 471, 1103
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 5 cut(s) 167, 207, 509, 692, 935
NciI CCSGG 3 cut(s) 102, 782, 783
NcoI CCATGG 1 cut(s) 1327
NdeII GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
NlaIII CATG 9 cut(s) 226, 346, 685, 752, 1030, 1100, 1120, 1325, 1331
NlaIV GGNNCC 2 cut(s) 875, 978
NmeAIII GCCGAG 1 cut(s) 1340
NmuCI GTSAC 4 cut(s) 214, 805, 1022, 1331
NsiI ATGCAT 1 cut(s) 683
NspV TTCGAA 3 cut(s) 841, 1056, 1087
PcsI WCGNNNNNNNCGW 1 cut(s) 1312
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 556, 967, 1058
PflFI GACNNNGTC 1 cut(s) 1028
PinAI ACCGGT 2 cut(s) 203, 1217
PkrI GCNGC 5 cut(s) 505, 697, 772, 856, 940
PleI GAGTC 2 cut(s) 92, 498
PpsI GAGTC 2 cut(s) 92, 498
PshAI GACNNNNGTC 1 cut(s) 1329
Psp6I CCWGG 1 cut(s) 463
PspEI GGTNACC 1 cut(s) 1331
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 2 cut(s) 875, 978
PspPI GGNCC 1 cut(s) 1068
PstNI CAGNNNCTG 1 cut(s) 1180
PsuI RGATCY 3 cut(s) 306, 655, 976
PsyI GACNNNGTC 1 cut(s) 1028
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 2 cut(s) 895, 919
RsaNI GTAC 2 cut(s) 894, 918
RseI CAYNNNNRTG 1 cut(s) 1326
SaqAI TTAA 1 cut(s) 1236
SatI GCNGC 5 cut(s) 504, 696, 771, 855, 939
Sau3AI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
Sau96I GGNCC 1 cut(s) 1068
SchI GAGTC 2 cut(s) 93, 499
ScrFI CCNGG 4 cut(s) 102, 465, 782, 783
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 1339
SfcI CTRYAG 2 cut(s) 1110, 1137
SfuI TTCGAA 3 cut(s) 841, 1056, 1087
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 1068
SmaI CCCGGG 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 1326
SmlI CTYRAG 1 cut(s) 1226
SmoI CTYRAG 1 cut(s) 1226
SsiI CCGC 3 cut(s) 25, 762, 1277
SspMI CTAG 2 cut(s) 738, 1354
StyD4I CCNGG 4 cut(s) 100, 463, 780, 781
StyI CCWWGG 2 cut(s) 1071, 1327
TaaI ACNGT 4 cut(s) 251, 299, 552, 1234
TaiI ACGT 1 cut(s) 1309
TaqI TCGA 6 cut(s) 180, 841, 1032, 1056, 1087, 1266
TatI WGTACW 1 cut(s) 917
TfiI GAWTC 3 cut(s) 556, 967, 1058
Tru1I TTAA 1 cut(s) 1236
Tru9I TTAA 1 cut(s) 1236
TscAI CASTG 4 cut(s) 235, 268, 514, 1015
TseFI GTSAC 4 cut(s) 214, 805, 1022, 1331
TseI GCWGC 5 cut(s) 503, 695, 770, 854, 938
Tsp45I GTSAC 4 cut(s) 214, 805, 1022, 1331
TspDTI ATGAA 6 cut(s) 255, 363, 959, 1133, 1220, 1269
TspMI CCCGGG 1 cut(s) 781
TspRI CASTG 4 cut(s) 235, 268, 514, 1015
Tth111I GACNNNGTC 1 cut(s) 1028
VpaK11BI GGWCC 1 cut(s) 1068
XapI RAATTY 5 cut(s) 403, 722, 743, 1089, 1120
XmaI CCCGGG 1 cut(s) 781
XspI CTAG 2 cut(s) 738, 1354
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.