Rmu_sc0000770.1_g000023

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000770.1
OG0019423
Physical Location & Seq
Reverse (-)
111854 .. 114041
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000770.1_g000023.1.cds

Sequence Viewer

Length: 1335 bp
atggagaaagcaagtgcattcctccgccatcctcctccttgtaagggaaaactcatcactatccttagcattgatggaggtggagtcaaagggattatccccggaactattctcgccgtcttagagaagaagcttcaggaaaaggagagcaatacaaatgcaagactggccgattatttcgatgtgattgcagggacgagcaccggtggccttgtgactgccatgctcactgctcctgatgaaaacaatcgtcccttgttcagtgctgaacagattgtccctttttatcttgaaaactgtcctaagatctttccaacaagcaagtctgcatttctaggcaacatggatgaattgccctctggttatagtgatgacacgcttcaacatttgaagctaatgccgaattcaggattttctcgtagttgtttattagagtggttatggaaattagttactaagcttcttcctggtccaaaatatgctgccactgcgttacagcatatagttgaaggaaaattaggagacatacggttgaatcagacattgaccaatgttgtaatcccgagttatgatataagtctgttccaaccggctatcttctcaacttacgagttgaaaaggaacgaggagcaagatccggctaaactctctgatatatgcatggcaacttcagcagcaccaatctattttcctgcatataaatttgaaaccaatactagagaattccatgtcgttgacggcggaatggctgctaataacccgggtttggttgctataaccgaagtgacaaaagaaatcaagaataaaaatccggactttagaaaagattgggcagctgatcagggcaatcggttcctacttatatcattgggtactggttcaccaaagcaaaagagttcatacaacgccgacgaagcagccaactggggtattgggggatggatgattcatttggatccattttcttgtccattgcaagaagtattcactgaagcaagtagtgacatggtcgatatccacctcactacccttttcgaatctatttggtccaagggaacttaccttcgaattcagcatgatcaattgcctgcagacatgaattctatggacaatgctaaagaagaaaacctgaaaaatcttgtgatagctgggcaggagctgttacagaaaccggtttcaaagatgaatacagctaccggtcgccttgagactgttaaacctgagatcacaaatgaaaaggctctcgaaatgatggctgaaaaactcgttgaggagaggaagaaacgtcaaagccgagaccgtgaccatggtcaccagaaggaaattgatgcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

444

Amino Acids

49.33

Weight (kDa)

5.71

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019423)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0075361
rosa_laevigata RLG00000036557
rosa_multiflora Rmu_sc0000770.1_g000023
rosa_roxburghii Rroxscaffold_1G00006140
rosa_rugosa Rorug05G0439100
rosa_samantha Rh5AG495700 Rh5BG517300
rosa_wichuraiana Rw5G046000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 811
AciI CCGC 2 cut(s) 25, 741
AclWI GGATC 3 cut(s) 629, 950, 963
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 5 cut(s) 403, 701, 722, 1068, 1099
AcuI CTGAAG 3 cut(s) 119, 654, 1011
AfaI GTAC 1 cut(s) 874
AfiI CCNNNNNNNGG 3 cut(s) 44, 407, 766
AgeI ACCGGT 3 cut(s) 203, 1171, 1196
AgsI TTSAA 8 cut(s) 293, 383, 391, 509, 535, 616, 707, 1179
AjnI CCWGG 1 cut(s) 466
AloI GAACNNNNNNTCC 2 cut(s) 261, 293
AluBI AGCT 7 cut(s) 133, 394, 460, 836, 1148, 1159, 1193
AluI AGCT 7 cut(s) 133, 394, 460, 836, 1148, 1159, 1193
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 516, 1202, 1290
AlwI GGATC 3 cut(s) 629, 950, 963
AlwNI CAGNNNCTG 1 cut(s) 1159
Ama87I CYCGRG 2 cut(s) 562, 760
Aor13HI TCCGGA 1 cut(s) 811
AoxI GGCC 2 cut(s) 168, 208
ApeKI GCWGC 5 cut(s) 482, 674, 749, 833, 917
ApoI RAATTY 5 cut(s) 403, 701, 722, 1068, 1099
AsiGI ACCGGT 3 cut(s) 203, 1171, 1196
AspS9I GGNCC 2 cut(s) 470, 1047
AsuC2I CCSGG 3 cut(s) 102, 761, 762
AsuHPI GGTGA 2 cut(s) 873, 1304
AsuII TTCGAA 2 cut(s) 1035, 1066
AvaI CYCGRG 2 cut(s) 562, 760
AvaII GGWCC 2 cut(s) 470, 1047
BamHI GGATCC 1 cut(s) 955
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 5 cut(s) 469, 686, 736, 845, 929
BccI CCATC 4 cut(s) 36, 68, 933, 1246
BceAI ACGGC 2 cut(s) 101, 754
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 468
BclI TGATCA 2 cut(s) 838, 1078
BcnI CCSGG 3 cut(s) 102, 761, 762
BcoDI GTCTC 3 cut(s) 516, 1202, 1290
BfaI CTAG 2 cut(s) 335, 717
BfmI CTRYAG 1 cut(s) 1089
BglII AGATCT 1 cut(s) 306
BisI GCNGC 5 cut(s) 483, 675, 750, 834, 918
BlsI GCNGC 5 cut(s) 484, 676, 751, 835, 919
Bme1390I CCNGG 4 cut(s) 102, 468, 761, 762
Bme18I GGWCC 2 cut(s) 470, 1047
BmeT110I CYCGRG 2 cut(s) 562, 760
BmgT120I GGNCC 2 cut(s) 470, 1047
BmiI GGNNCC 2 cut(s) 854, 957
BmrFI CCNGG 4 cut(s) 102, 468, 761, 762
BmrI ACTGGG 1 cut(s) 934
BmsI GCATC 1 cut(s) 1318
BmuI ACTGGG 1 cut(s) 934
BoxI GACNNNNGTC 1 cut(s) 1308
Bpu10I CCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 2 cut(s) 1035, 1066
BpuEI CTTGAG 1 cut(s) 1226
BpuMI CCSGG 3 cut(s) 102, 761, 762
BsaI GGTCTC 1 cut(s) 1290
BsaJI CCNNGG 4 cut(s) 100, 760, 1050, 1306
BsaWI WCCGGW 4 cut(s) 203, 811, 1171, 1196
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 407, 766
Bse118I RCCGGY 4 cut(s) 203, 589, 1171, 1196
Bse1I ACTGG 3 cut(s) 171, 880, 929
Bse3DI GCAATG 1 cut(s) 971
BseAI TCCGGA 1 cut(s) 811
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 4 cut(s) 100, 760, 1050, 1306
BseGI GGATG 4 cut(s) 28, 352, 944, 948
BseLI CCNNNNNNNGG 3 cut(s) 44, 407, 766
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 1 cut(s) 1212
BseNI ACTGG 3 cut(s) 171, 880, 929
BseRI GAGGAG 3 cut(s) 24, 641, 1286
BseXI GCAGC 5 cut(s) 469, 686, 736, 845, 929
BseYI CCCAGC 1 cut(s) 1148
Bsh1285I CGRYCG 1 cut(s) 1201
BshFI GGCC 2 cut(s) 170, 210
BshTI ACCGGT 3 cut(s) 203, 1171, 1196
BsiEI CGRYCG 1 cut(s) 1201
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 2 cut(s) 562, 760
BsiSI CCGG 8 cut(s) 102, 204, 590, 638, 761, 812, 1172, 1197
BslFI GGGAC 3 cut(s) 208, 237, 263
BslI CCNNNNNNNGG 3 cut(s) 44, 407, 766
BsmAI GTCTC 3 cut(s) 516, 1202, 1290
BsmFI GGGAC 3 cut(s) 208, 237, 263
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 170, 210
Bso31I GGTCTC 1 cut(s) 1290
BsoBI CYCGRG 2 cut(s) 562, 760
Bsp119I TTCGAA 2 cut(s) 1035, 1066
Bsp1286I GDGCHC 1 cut(s) 203
Bsp13I TCCGGA 1 cut(s) 811
Bsp143I GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
Bsp19I CCATGG 1 cut(s) 1306
BspACI CCGC 2 cut(s) 25, 741
BspANI GGCC 2 cut(s) 170, 210
BspCNI CTCAG 1 cut(s) 1213
BspEI TCCGGA 1 cut(s) 811
BspLI GGNNCC 2 cut(s) 854, 957
BspMAI CTGCAG 1 cut(s) 1093
BspPI GGATC 3 cut(s) 629, 950, 963
BspT104I TTCGAA 2 cut(s) 1035, 1066
BspTNI GGTCTC 1 cut(s) 1290
BsrDI GCAATG 1 cut(s) 971
BsrFI RCCGGY 4 cut(s) 203, 589, 1171, 1196
BsrI ACTGG 3 cut(s) 171, 880, 929
BssAI RCCGGY 4 cut(s) 203, 589, 1171, 1196
BssECI CCNNGG 4 cut(s) 100, 760, 1050, 1306
BssMI GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
BssT1I CCWWGG 2 cut(s) 1050, 1306
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 4 cut(s) 299, 531, 1213, 1301
BstBI TTCGAA 2 cut(s) 1035, 1066
BstC8I GCNNGC 1 cut(s) 1089
BstDEI CTNAG 5 cut(s) 65, 121, 303, 456, 1221
BstDSI CCRYGG 1 cut(s) 1306
BstEII GGTNACC 1 cut(s) 1310
BstF5I GGATG 4 cut(s) 28, 352, 944, 948
BstKTI GATC 6 cut(s) 309, 637, 841, 958, 1081, 1227
BstMAI GTCTC 3 cut(s) 516, 1202, 1290
BstMBI GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
BstMCI CGRYCG 1 cut(s) 1201
BstMWI GCNNNNNNNGC 5 cut(s) 167, 207, 488, 671, 914
BstNI CCWGG 1 cut(s) 468
BstPAI GACNNNNGTC 1 cut(s) 1308
BstPI GGTNACC 1 cut(s) 1310
BstSCI CCNGG 4 cut(s) 100, 466, 759, 760
BstSFI CTRYAG 1 cut(s) 1089
BstV1I GCAGC 5 cut(s) 469, 686, 736, 845, 929
BstX2I RGATCY 3 cut(s) 306, 634, 955
BstYI RGATCY 3 cut(s) 306, 634, 955
BsuRI GGCC 2 cut(s) 170, 210
BtgI CCRYGG 1 cut(s) 1306
BtsCI GGATG 4 cut(s) 28, 352, 944, 948
BtsI GCAGTG 2 cut(s) 228, 486
BtsIMutI CAGTG 4 cut(s) 228, 268, 486, 987
Cac8I GCNNGC 1 cut(s) 1089
CaiI CAGNNNCTG 1 cut(s) 1159
Cfr10I RCCGGY 4 cut(s) 203, 589, 1171, 1196
Cfr13I GGNCC 2 cut(s) 470, 1047
Cfr9I CCCGGG 1 cut(s) 760
Csp6I GTAC 1 cut(s) 873
CspAI ACCGGT 3 cut(s) 203, 1171, 1196
CviAII CATG 8 cut(s) 223, 343, 661, 728, 1006, 1076, 1096, 1307
CviQI GTAC 1 cut(s) 873
DdeI CTNAG 5 cut(s) 65, 121, 303, 456, 1221
DpnI GATC 6 cut(s) 308, 636, 840, 957, 1080, 1226
DpnII GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
EaeI YGGCCR 1 cut(s) 168
EciI GGCGGA 2 cut(s) 14, 756
Eco130I CCWWGG 2 cut(s) 1050, 1306
Eco31I GGTCTC 1 cut(s) 1290
Eco32I GATATC 1 cut(s) 1015
Eco47I GGWCC 2 cut(s) 470, 1047
Eco57I CTGAAG 3 cut(s) 119, 654, 1011
Eco88I CYCGRG 2 cut(s) 562, 760
Eco91I GGTNACC 1 cut(s) 1310
EcoO65I GGTNACC 1 cut(s) 1310
EcoRI GAATTC 4 cut(s) 403, 722, 1068, 1099
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 2 cut(s) 1050, 1306
EcoT22I ATGCAT 1 cut(s) 662
ErhI CCWWGG 2 cut(s) 1050, 1306
FaeI CATG 8 cut(s) 226, 346, 664, 731, 1009, 1079, 1099, 1310
FaqI GGGAC 3 cut(s) 208, 237, 263
FatI CATG 8 cut(s) 222, 342, 660, 727, 1005, 1075, 1095, 1306
FbaI TGATCA 2 cut(s) 838, 1078
Fnu4HI GCNGC 5 cut(s) 483, 675, 750, 834, 918
FokI GGATG 4 cut(s) 15, 359, 951, 955
Fsp4HI GCNGC 5 cut(s) 483, 675, 750, 834, 918
FspBI CTAG 2 cut(s) 335, 717
GluI GCNGC 5 cut(s) 483, 675, 750, 834, 918
GsaI CCCAGC 1 cut(s) 1152
HaeIII GGCC 2 cut(s) 170, 210
HapII CCGG 8 cut(s) 102, 204, 590, 638, 761, 812, 1172, 1197
Hin1II CATG 8 cut(s) 226, 346, 664, 731, 1009, 1079, 1099, 1310
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 2 cut(s) 131, 458
HinfI GANTC 4 cut(s) 84, 535, 946, 1037
HpaII CCGG 8 cut(s) 102, 204, 590, 638, 761, 812, 1172, 1197
HphI GGTGA 2 cut(s) 873, 1304
Hpy166II GTNNAC 2 cut(s) 736, 881
Hpy188I TCNGA 2 cut(s) 540, 652
Hpy188III TCNNGA 8 cut(s) 137, 236, 290, 408, 562, 799, 812, 1244
Hpy8I GTNNAC 2 cut(s) 736, 881
Hpy99I CGWCG 1 cut(s) 914
HpyAV CCTTC 3 cut(s) 503, 1073, 1312
HpyCH4III ACNGT 4 cut(s) 299, 531, 1213, 1301
HpyCH4IV ACGT 1 cut(s) 1285
HpyCH4V TGCA 8 cut(s) 17, 161, 191, 329, 660, 695, 976, 1091
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 207, 488, 671, 914
HpyF3I CTNAG 5 cut(s) 65, 121, 303, 456, 1221
HpySE526I ACGT 1 cut(s) 1285
Hsp92II CATG 8 cut(s) 226, 346, 664, 731, 1009, 1079, 1099, 1310
Kpn2I TCCGGA 1 cut(s) 811
Ksp22I TGATCA 2 cut(s) 838, 1078
Kzo9I GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
LmnI GCTCC 3 cut(s) 238, 628, 1156
Lsp1109I GCAGC 5 cut(s) 469, 686, 736, 845, 929
LweI GCATC 1 cut(s) 1318
MaeI CTAG 2 cut(s) 335, 717
MaeII ACGT 1 cut(s) 1285
MaeIII GTNAC 8 cut(s) 214, 451, 492, 784, 1001, 1161, 1301, 1310
MalI GATC 6 cut(s) 308, 636, 840, 957, 1080, 1226
MboI GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
MboII GAAGA 5 cut(s) 139, 455, 589, 1133, 1291
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 3 cut(s) 306, 634, 955
MhlI GDGCHC 1 cut(s) 203
MlyI GAGTC 1 cut(s) 93
MmeI TCCRAC 2 cut(s) 338, 610
MnlI CCTC 9 cut(s) 32, 42, 45, 71, 367, 619, 1031, 1264, 1269
Mph1103I ATGCAT 1 cut(s) 662
MroI TCCGGA 1 cut(s) 811
MseI TTAA 1 cut(s) 1215
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 8 cut(s) 102, 204, 590, 638, 761, 812, 1172, 1197
MspR9I CCNGG 4 cut(s) 102, 468, 761, 762
MunI CAATTG 1 cut(s) 1082
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 5 cut(s) 167, 207, 488, 671, 914
NciI CCSGG 3 cut(s) 102, 761, 762
NcoI CCATGG 1 cut(s) 1306
NdeII GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
NlaIII CATG 8 cut(s) 226, 346, 664, 731, 1009, 1079, 1099, 1310
NlaIV GGNNCC 2 cut(s) 854, 957
NmeAIII GCCGAG 1 cut(s) 1319
NmuCI GTSAC 5 cut(s) 214, 784, 1001, 1301, 1310
NsiI ATGCAT 1 cut(s) 662
NspV TTCGAA 2 cut(s) 1035, 1066
PcsI WCGNNNNNNNCGW 1 cut(s) 1291
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 535, 946, 1037
PflFI GACNNNGTC 1 cut(s) 1007
PinAI ACCGGT 3 cut(s) 203, 1171, 1196
PkrI GCNGC 5 cut(s) 484, 676, 751, 835, 919
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PshAI GACNNNNGTC 1 cut(s) 1308
Psp6I CCWGG 1 cut(s) 466
PspEI GGTNACC 1 cut(s) 1310
PspFI CCCAGC 1 cut(s) 1148
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 854, 957
PspPI GGNCC 2 cut(s) 470, 1047
PstI CTGCAG 1 cut(s) 1093
PstNI CAGNNNCTG 1 cut(s) 1159
PsuI RGATCY 3 cut(s) 306, 634, 955
PsyI GACNNNGTC 1 cut(s) 1007
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 1 cut(s) 874
RsaNI GTAC 1 cut(s) 873
SaqAI TTAA 1 cut(s) 1215
SatI GCNGC 5 cut(s) 483, 675, 750, 834, 918
Sau3AI GATC 6 cut(s) 306, 634, 838, 955, 1078, 1224
Sau96I GGNCC 2 cut(s) 470, 1047
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 4 cut(s) 102, 468, 761, 762
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 1318
SfcI CTRYAG 1 cut(s) 1089
SfuI TTCGAA 2 cut(s) 1035, 1066
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 2 cut(s) 470, 1047
SmaI CCCGGG 1 cut(s) 762
SmlI CTYRAG 1 cut(s) 1205
SmoI CTYRAG 1 cut(s) 1205
SsiI CCGC 2 cut(s) 25, 741
SspMI CTAG 2 cut(s) 335, 717
StyD4I CCNGG 4 cut(s) 100, 466, 759, 760
StyI CCWWGG 2 cut(s) 1050, 1306
TaaI ACNGT 4 cut(s) 299, 531, 1213, 1301
TaiI ACGT 1 cut(s) 1288
TaqI TCGA 5 cut(s) 180, 1011, 1035, 1066, 1245
TfiI GAWTC 3 cut(s) 535, 946, 1037
Tru1I TTAA 1 cut(s) 1215
Tru9I TTAA 1 cut(s) 1215
TscAI CASTG 4 cut(s) 235, 268, 493, 994
TseFI GTSAC 5 cut(s) 214, 784, 1001, 1301, 1310
TseI GCWGC 5 cut(s) 482, 674, 749, 833, 917
Tsp45I GTSAC 5 cut(s) 214, 784, 1001, 1301, 1310
TspDTI ATGAA 7 cut(s) 255, 363, 888, 938, 1112, 1199, 1248
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 4 cut(s) 235, 268, 493, 994
Tth111I GACNNNGTC 1 cut(s) 1007
VpaK11BI GGWCC 2 cut(s) 470, 1047
XapI RAATTY 5 cut(s) 403, 701, 722, 1068, 1099
XmaI CCCGGG 1 cut(s) 760
XspI CTAG 2 cut(s) 335, 717
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.