Rroxscaffold_1G00006140

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019423
Physical Location & Seq
Reverse (-)
8163852 .. 8166249
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006140.1

Sequence Viewer

Length: 1299 bp
ATGGAGAAAGCAAGTGCATTCCTCCGCCATCCTCCTCCTTGTAAGGGAAAACTCATCACTATCCTTAGCATTGATGGAGGTGGAGTCAAAGGGATTATCCCCGGAACTATTCTCGCCGTCTTAGAGAAGAAGCTTCAGGAAAAGGAGAGCAATACAAATGCAAGACTGGCCGATTATTTCGATGTGATTGCAGGGACGAGCACCGGTGGCCTTGTGACTGCCATGCTCACTGCTCCTGATGAAAACAACCGTCCCTTGTTCAGTGCTGACCAGATTGTCCCTTTTTATCTTGAAAACTGTCCTATGATCTTTCCACCAAGCAAGTCTGCATTTCTAGGCAACATGGATGAATTGCCCTCTGGTATTAGTGATGACACACTTCAACATTTGAAGCTAATGCCGAATTCAGGATTTTCTCGTAGTTGGTTAGAGTGGTTATGGAAATTAGTTAAGCTTCTTCCAGGTTCAATTGGGAAGCTACTGGGGAGTCCAAAATATGCTGCCACTGTGTTACGGGATATAGTTGAAGGAAAATTAGGAGACATACGGTTGAATCAGACATTGACCAATTTGAAAAGGAACGAGGAGCAAGATCCGGCTAAACTCTCTGATATATGCATGGCAACTTCAGCAGCACCAACCTATTTTCCTGCATATAAATTTGCAACCAATACTAGAGAATTCCATGTCGTTGACGGCGGAATGGCTGCTAATAACCCGGTACTAGGTTTGGTTGCTATAACCGAAGTGACAAAAGAAATCAAGAATAAAAATCCTGACTTTCGAAAAGATTGGGCAGCTGATCAGGGCAATCGGTTCCTACTTATATCATTGGGTACTGGTTCACCAAAGCAAAAGAGTACATACAACGCCGACGAAGCAGCCAACTGGGGTATTGGGGGATGGATGATTCATTTGGATCCATTTTCTTGTCCATTGCAAGAAGTATTCACTGAAGCAAGTAGTGACATGGTCGATATCCACCTCACTACGCTTTTCGAATCTATTTGGTCCAAGGGAACTTACCTTCGAATTCAGCATGATCAATTGCCTACAGACATGAATTCTATGGACAATGCTACAGAAGAAAACCTGAAAAATCTTGTGATAGCTGGGCAGGAGCTGTTACAGAAATCGGTTTCAAAGATGAATACAGCTACCGGTCGCCTTGAGACTGTTAAACCTGAGATCACAAATGAAAAGGCTCTCGAAATGATGGCGGAAAAACTCGTTGAGGAGAGGAAGAAACGTCAAAGCCGAGACCATGACCATGGTCACCAGAAGGAAATTGATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

432

Amino Acids

47.79

Weight (kDa)

5.81

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 243 5.3e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019423)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0075361
rosa_laevigata RLG00000036557
rosa_multiflora Rmu_sc0000770.1_g000023
rosa_roxburghii Rroxscaffold_1G00006140
rosa_rugosa Rorug05G0439100
rosa_samantha Rh5AG495700 Rh5BG517300
rosa_wichuraiana Rw5G046000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AciI CCGC 3 cut(s) 25, 699, 1220
AclWI GGATC 3 cut(s) 587, 914, 927
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 5 cut(s) 403, 659, 680, 1032, 1063
AcuI CTGAAG 3 cut(s) 119, 612, 975
AfaI GTAC 3 cut(s) 723, 838, 862
AfiI CCNNNNNNNGG 3 cut(s) 44, 407, 725
AgeI ACCGGT 2 cut(s) 203, 1160
AgsI TTSAA 8 cut(s) 293, 383, 391, 468, 527, 553, 574, 1143
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 8 cut(s) 133, 394, 454, 478, 800, 1112, 1123, 1157
AluI AGCT 8 cut(s) 133, 394, 454, 478, 800, 1112, 1123, 1157
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 534, 1166, 1254
AlwI GGATC 3 cut(s) 587, 914, 927
AlwNI CAGNNNCTG 1 cut(s) 1123
AoxI GGCC 2 cut(s) 168, 208
ApeKI GCWGC 5 cut(s) 500, 632, 707, 797, 881
ApoI RAATTY 5 cut(s) 403, 659, 680, 1032, 1063
AsiGI ACCGGT 2 cut(s) 203, 1160
AspS9I GGNCC 1 cut(s) 1011
AsuC2I CCSGG 2 cut(s) 102, 719
AsuHPI GGTGA 2 cut(s) 837, 1268
AsuII TTCGAA 3 cut(s) 784, 999, 1030
AvaII GGWCC 1 cut(s) 1011
BamHI GGATCC 1 cut(s) 919
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 5 cut(s) 487, 644, 694, 809, 893
BccI CCATC 4 cut(s) 36, 68, 897, 1210
BceAI ACGGC 2 cut(s) 101, 712
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 462
BclI TGATCA 2 cut(s) 802, 1042
BcnI CCSGG 2 cut(s) 102, 719
BcoDI GTCTC 3 cut(s) 534, 1166, 1254
BfaI CTAG 3 cut(s) 335, 675, 725
BfmI CTRYAG 2 cut(s) 1053, 1080
BisI GCNGC 5 cut(s) 501, 633, 708, 798, 882
BlsI GCNGC 5 cut(s) 502, 634, 709, 799, 883
Bme1390I CCNGG 3 cut(s) 102, 462, 719
Bme18I GGWCC 1 cut(s) 1011
BmgT120I GGNCC 1 cut(s) 1011
BmiI GGNNCC 2 cut(s) 818, 921
BmrFI CCNGG 3 cut(s) 102, 462, 719
BmrI ACTGGG 2 cut(s) 491, 898
BmsI GCATC 1 cut(s) 1282
BmuI ACTGGG 2 cut(s) 491, 898
BoxI GACNNNNGTC 1 cut(s) 1272
Bpu10I CCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 3 cut(s) 784, 999, 1030
BpuEI CTTGAG 1 cut(s) 1190
BpuMI CCSGG 2 cut(s) 102, 719
BsaI GGTCTC 1 cut(s) 1254
BsaJI CCNNGG 3 cut(s) 100, 1014, 1270
BsaWI WCCGGW 2 cut(s) 203, 1160
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 407, 725
Bse118I RCCGGY 2 cut(s) 203, 1160
Bse1I ACTGG 4 cut(s) 171, 486, 844, 893
Bse3DI GCAATG 1 cut(s) 935
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 3 cut(s) 100, 1014, 1270
BseGI GGATG 4 cut(s) 28, 352, 908, 912
BseLI CCNNNNNNNGG 3 cut(s) 44, 407, 725
BseMI GCAATG 1 cut(s) 935
BseMII CTCAG 1 cut(s) 1176
BseNI ACTGG 4 cut(s) 171, 486, 844, 893
BseRI GAGGAG 3 cut(s) 24, 599, 1250
BseXI GCAGC 5 cut(s) 487, 644, 694, 809, 893
BseYI CCCAGC 1 cut(s) 1112
Bsh1285I CGRYCG 1 cut(s) 1165
BshFI GGCC 2 cut(s) 170, 210
BshTI ACCGGT 2 cut(s) 203, 1160
BsiEI CGRYCG 1 cut(s) 1165
BsiHKAI GWGCWC 1 cut(s) 203
BsiSI CCGG 5 cut(s) 102, 204, 596, 719, 1161
BslFI GGGAC 3 cut(s) 208, 237, 263
BslI CCNNNNNNNGG 3 cut(s) 44, 407, 725
BsmAI GTCTC 3 cut(s) 534, 1166, 1254
BsmFI GGGAC 3 cut(s) 208, 237, 263
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 170, 210
Bso31I GGTCTC 1 cut(s) 1254
Bsp119I TTCGAA 3 cut(s) 784, 999, 1030
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
Bsp19I CCATGG 1 cut(s) 1270
BspACI CCGC 3 cut(s) 25, 699, 1220
BspANI GGCC 2 cut(s) 170, 210
BspCNI CTCAG 1 cut(s) 1177
BspLI GGNNCC 2 cut(s) 818, 921
BspPI GGATC 3 cut(s) 587, 914, 927
BspT104I TTCGAA 3 cut(s) 784, 999, 1030
BspTNI GGTCTC 1 cut(s) 1254
BsrDI GCAATG 1 cut(s) 935
BsrFI RCCGGY 2 cut(s) 203, 1160
BsrI ACTGG 4 cut(s) 171, 486, 844, 893
BssAI RCCGGY 2 cut(s) 203, 1160
BssECI CCNNGG 3 cut(s) 100, 1014, 1270
BssMI GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
BssT1I CCWWGG 2 cut(s) 1014, 1270
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 5 cut(s) 251, 299, 508, 549, 1177
BstBI TTCGAA 3 cut(s) 784, 999, 1030
BstDEI CTNAG 3 cut(s) 65, 121, 1185
BstDSI CCRYGG 1 cut(s) 1270
BstEII GGTNACC 1 cut(s) 1274
BstF5I GGATG 4 cut(s) 28, 352, 908, 912
BstKTI GATC 6 cut(s) 309, 595, 805, 922, 1045, 1191
BstMAI GTCTC 3 cut(s) 534, 1166, 1254
BstMBI GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
BstMCI CGRYCG 1 cut(s) 1165
BstMWI GCNNNNNNNGC 4 cut(s) 167, 207, 629, 878
BstNI CCWGG 1 cut(s) 462
BstPAI GACNNNNGTC 1 cut(s) 1272
BstPI GGTNACC 1 cut(s) 1274
BstSCI CCNGG 3 cut(s) 100, 460, 717
BstSFI CTRYAG 2 cut(s) 1053, 1080
BstV1I GCAGC 5 cut(s) 487, 644, 694, 809, 893
BstX2I RGATCY 2 cut(s) 592, 919
BstXI CCANNNNNNTGG 1 cut(s) 1271
BstYI RGATCY 2 cut(s) 592, 919
BsuRI GGCC 2 cut(s) 170, 210
BtgI CCRYGG 1 cut(s) 1270
BtsCI GGATG 4 cut(s) 28, 352, 908, 912
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 4 cut(s) 228, 268, 504, 951
CaiI CAGNNNCTG 1 cut(s) 1123
Cfr10I RCCGGY 2 cut(s) 203, 1160
Cfr13I GGNCC 1 cut(s) 1011
Csp6I GTAC 3 cut(s) 722, 837, 861
CspAI ACCGGT 2 cut(s) 203, 1160
CviAII CATG 9 cut(s) 223, 343, 619, 686, 970, 1040, 1060, 1265, 1271
CviQI GTAC 3 cut(s) 722, 837, 861
DdeI CTNAG 3 cut(s) 65, 121, 1185
DpnI GATC 6 cut(s) 308, 594, 804, 921, 1044, 1190
DpnII GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 168
EciI GGCGGA 3 cut(s) 14, 714, 1235
Eco130I CCWWGG 2 cut(s) 1014, 1270
Eco31I GGTCTC 1 cut(s) 1254
Eco32I GATATC 1 cut(s) 979
Eco47I GGWCC 1 cut(s) 1011
Eco57I CTGAAG 3 cut(s) 119, 612, 975
Eco91I GGTNACC 1 cut(s) 1274
EcoO65I GGTNACC 1 cut(s) 1274
EcoRI GAATTC 4 cut(s) 403, 680, 1032, 1063
EcoRII CCWGG 1 cut(s) 460
EcoRV GATATC 1 cut(s) 979
EcoT14I CCWWGG 2 cut(s) 1014, 1270
EcoT22I ATGCAT 1 cut(s) 620
ErhI CCWWGG 2 cut(s) 1014, 1270
FaeI CATG 9 cut(s) 226, 346, 622, 689, 973, 1043, 1063, 1268, 1274
FaqI GGGAC 3 cut(s) 208, 237, 263
FatI CATG 9 cut(s) 222, 342, 618, 685, 969, 1039, 1059, 1264, 1270
FbaI TGATCA 2 cut(s) 802, 1042
Fnu4HI GCNGC 5 cut(s) 501, 633, 708, 798, 882
FokI GGATG 4 cut(s) 15, 359, 915, 919
Fsp4HI GCNGC 5 cut(s) 501, 633, 708, 798, 882
FspBI CTAG 3 cut(s) 335, 675, 725
GluI GCNGC 5 cut(s) 501, 633, 708, 798, 882
GsaI CCCAGC 1 cut(s) 1116
HaeIII GGCC 2 cut(s) 170, 210
HapII CCGG 5 cut(s) 102, 204, 596, 719, 1161
Hin1II CATG 9 cut(s) 226, 346, 622, 689, 973, 1043, 1063, 1268, 1274
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HindIII AAGCTT 2 cut(s) 131, 452
HinfI GANTC 5 cut(s) 84, 487, 553, 910, 1001
HpaII CCGG 5 cut(s) 102, 204, 596, 719, 1161
HphI GGTGA 2 cut(s) 837, 1268
Hpy166II GTNNAC 2 cut(s) 694, 845
Hpy188I TCNGA 2 cut(s) 558, 610
Hpy188III TCNNGA 7 cut(s) 137, 236, 290, 408, 763, 776, 1208
Hpy8I GTNNAC 2 cut(s) 694, 845
Hpy99I CGWCG 1 cut(s) 878
HpyAV CCTTC 3 cut(s) 521, 1037, 1276
HpyCH4III ACNGT 5 cut(s) 251, 299, 508, 549, 1177
HpyCH4IV ACGT 1 cut(s) 1249
HpyCH4V TGCA 8 cut(s) 17, 161, 191, 329, 618, 653, 665, 940
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 207, 629, 878
HpyF3I CTNAG 3 cut(s) 65, 121, 1185
HpySE526I ACGT 1 cut(s) 1249
Hsp92II CATG 9 cut(s) 226, 346, 622, 689, 973, 1043, 1063, 1268, 1274
Ksp22I TGATCA 2 cut(s) 802, 1042
Kzo9I GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
LmnI GCTCC 3 cut(s) 238, 586, 1120
Lsp1109I GCAGC 5 cut(s) 487, 644, 694, 809, 893
LweI GCATC 1 cut(s) 1282
MaeI CTAG 3 cut(s) 335, 675, 725
MaeII ACGT 1 cut(s) 1249
MaeIII GTNAC 6 cut(s) 214, 510, 748, 965, 1125, 1274
MalI GATC 6 cut(s) 308, 594, 804, 921, 1044, 1190
MboI GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
MboII GAAGA 4 cut(s) 139, 449, 1097, 1255
MfeI CAATTG 2 cut(s) 468, 1046
MflI RGATCY 2 cut(s) 592, 919
MhlI GDGCHC 1 cut(s) 203
MlyI GAGTC 2 cut(s) 93, 496
MnlI CCTC 9 cut(s) 32, 42, 45, 71, 367, 577, 995, 1228, 1233
Mph1103I ATGCAT 1 cut(s) 620
MseI TTAA 2 cut(s) 450, 1179
MslI CAYNNNNRTG 1 cut(s) 1269
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 5 cut(s) 102, 204, 596, 719, 1161
MspR9I CCNGG 3 cut(s) 102, 462, 719
MunI CAATTG 2 cut(s) 468, 1046
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 4 cut(s) 167, 207, 629, 878
NciI CCSGG 2 cut(s) 102, 719
NcoI CCATGG 1 cut(s) 1270
NdeII GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
NlaIII CATG 9 cut(s) 226, 346, 622, 689, 973, 1043, 1063, 1268, 1274
NlaIV GGNNCC 2 cut(s) 818, 921
NmeAIII GCCGAG 1 cut(s) 1283
NmuCI GTSAC 4 cut(s) 214, 748, 965, 1274
NsiI ATGCAT 1 cut(s) 620
NspV TTCGAA 3 cut(s) 784, 999, 1030
PcsI WCGNNNNNNNCGW 1 cut(s) 1255
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 553, 910, 1001
PflFI GACNNNGTC 1 cut(s) 971
PinAI ACCGGT 2 cut(s) 203, 1160
PkrI GCNGC 5 cut(s) 502, 634, 709, 799, 883
PleI GAGTC 2 cut(s) 92, 495
PpsI GAGTC 2 cut(s) 92, 495
PshAI GACNNNNGTC 1 cut(s) 1272
Psp6I CCWGG 1 cut(s) 460
PspEI GGTNACC 1 cut(s) 1274
PspFI CCCAGC 1 cut(s) 1112
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 818, 921
PspPI GGNCC 1 cut(s) 1011
PstNI CAGNNNCTG 1 cut(s) 1123
PsuI RGATCY 2 cut(s) 592, 919
PsyI GACNNNGTC 1 cut(s) 971
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 3 cut(s) 723, 838, 862
RsaNI GTAC 3 cut(s) 722, 837, 861
RseI CAYNNNNRTG 1 cut(s) 1269
SaqAI TTAA 2 cut(s) 450, 1179
SatI GCNGC 5 cut(s) 501, 633, 708, 798, 882
Sau3AI GATC 6 cut(s) 306, 592, 802, 919, 1042, 1188
Sau96I GGNCC 1 cut(s) 1011
SchI GAGTC 2 cut(s) 93, 496
ScrFI CCNGG 3 cut(s) 102, 462, 719
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 1282
SfcI CTRYAG 2 cut(s) 1053, 1080
SfuI TTCGAA 3 cut(s) 784, 999, 1030
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 1011
SmiMI CAYNNNNRTG 1 cut(s) 1269
SmlI CTYRAG 1 cut(s) 1169
SmoI CTYRAG 1 cut(s) 1169
SsiI CCGC 3 cut(s) 25, 699, 1220
SspMI CTAG 3 cut(s) 335, 675, 725
StyD4I CCNGG 3 cut(s) 100, 460, 717
StyI CCWWGG 2 cut(s) 1014, 1270
TaaI ACNGT 5 cut(s) 251, 299, 508, 549, 1177
TaiI ACGT 1 cut(s) 1252
TaqI TCGA 6 cut(s) 180, 784, 975, 999, 1030, 1209
TatI WGTACW 1 cut(s) 860
TfiI GAWTC 3 cut(s) 553, 910, 1001
Tru1I TTAA 2 cut(s) 450, 1179
Tru9I TTAA 2 cut(s) 450, 1179
TscAI CASTG 4 cut(s) 235, 268, 511, 958
TseFI GTSAC 4 cut(s) 214, 748, 965, 1274
TseI GCWGC 5 cut(s) 500, 632, 707, 797, 881
Tsp45I GTSAC 4 cut(s) 214, 748, 965, 1274
TspDTI ATGAA 6 cut(s) 255, 363, 902, 1076, 1163, 1212
TspRI CASTG 4 cut(s) 235, 268, 511, 958
Tth111I GACNNNGTC 1 cut(s) 971
VpaK11BI GGWCC 1 cut(s) 1011
XapI RAATTY 5 cut(s) 403, 659, 680, 1032, 1063
XspI CTAG 3 cut(s) 335, 675, 725
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.