Rh5BG517300

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019423
Physical Location & Seq
Forward (+)
81883222 .. 81885603
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG517300.1

Sequence Viewer

Length: 1365 bp
ATGGAGAAAGCAAGTGCATTCCTCCGCCATCCTCCTCCTTGTAAGGGAAAACTCATCACTATCCTTAGCATTGATGGAGGTGGAGTCAAAGGGATTATCCCCGGAACTATTCTCGCCGTCTTAGAGAAGAAGCTTCAGGAAAAGGAGAGCAATACAAATGCAAGACTGGCCGATTATTTCGATGTGATTGCAGGGACGAGCACCGGTGGCCTTGTGACTGCCATGCTCACTGCTCCTGATGAAAACAATCGTCCCTTGTTCAGTGCTGACCAGATTGTCCCTTTTTATCTTGAAAACTGTCCTAAGATCTTTCCACCAAGCAAGTCTGCATTTCTAGGCAACATGGATGAATTGCCCTCTGGTTATAGTGATGACACGCTTCAGCATTTGAAGCTAATGCCGAATTCAGTATTTTCTCGTAGTTGGTTAGAGTGGTTATGGAAATTAGTTACTAAGCTTCTTCCTGGTTCAATTGGGAAGCTACTGGGAAGTCCAAAATATGCTGCCACTGCGTTACGGCATATAGTTGAAGGAAAATTAGGAGACATACGGTTGAATCAGACATTGACCAATGTTGTAATCCCGAGTTATGATATAAGTCTGCTCCAACCAGCTATCTTCTCAACTTACGAGTTGAAAAGGAACGAGGAGCAAGATCCGGCTAAACTCTCTGACATATGCATGGCAACTTCAGCAGCACCAACCTATTTTCCTGCATATAAATTTGAAACCAATACTAGAGAATTCCATGTCGTTGACGGCGGAATGGCTGCTAATAACCCGGGTTTGGTTGCTATAACCGAAGTGACAAAAGAAATCAAGAATAAAAATCCTGACTTTCGAAAAGATTGGGCAGCTGATCAGGGCAATCGGTTCCTACTTATATCATTGGGTACTGGTTCACCAAAGCAAAAGAGTTCATACAACGCTGACGAAGCAGCCAACTGGGGTATTGGGGGATGGATGATTCATTTGGATCCATTTTCTTGTCCATTGCAAGAAGTATTCACTGAAGCAAGTAGTGACATGGTCGATATCCACCTCACTACCCTTTTCGAATCTATTTGGTCCAAGGGAACTTACCTTCGAATTCAGCATGATCAATTGCCTGCAGACATGAATTCTATGGACAATGCTAAAGAAGAAAACCTGAAAAATCTTGTGATAGCTGGGCAGGAGCTGTTACAGAAACCGCTTTCAAAGATGAATACAGCTACCGGTCGCCTTGAGACTGTTAAACCTGAGATCACAAATGAAAAGGCTCTCGAAATGTATTATTACTCTCCATCAATACTAAGATTCATAGCAAAACATACATCTTATTTCTATATATTCGTTGTAAAAGCACTAATACTAGCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

454

Amino Acids

50.44

Weight (kDa)

6.12

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 265 1.8e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019423)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0075361
rosa_laevigata RLG00000036557
rosa_multiflora Rmu_sc0000770.1_g000023
rosa_roxburghii Rroxscaffold_1G00006140
rosa_rugosa Rorug05G0439100
rosa_samantha Rh5AG495700 Rh5BG517300
rosa_wichuraiana Rw5G046000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AciI CCGC 3 cut(s) 25, 762, 1193
AclWI GGATC 3 cut(s) 650, 971, 984
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 5 cut(s) 403, 722, 743, 1089, 1120
AcuI CTGAAG 4 cut(s) 119, 365, 675, 1032
AfaI GTAC 1 cut(s) 895
AfiI CCNNNNNNNGG 2 cut(s) 44, 787
AgeI ACCGGT 2 cut(s) 203, 1217
AgsI TTSAA 8 cut(s) 293, 391, 471, 530, 556, 637, 728, 1200
AjnI CCWGG 1 cut(s) 463
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 2 cut(s) 537, 1223
AlwI GGATC 3 cut(s) 650, 971, 984
AlwNI CAGNNNCTG 1 cut(s) 1180
Ama87I CYCGRG 2 cut(s) 583, 781
AoxI GGCC 2 cut(s) 168, 208
ApeKI GCWGC 5 cut(s) 503, 695, 770, 854, 938
ApoI RAATTY 5 cut(s) 403, 722, 743, 1089, 1120
AsiGI ACCGGT 2 cut(s) 203, 1217
AspS9I GGNCC 1 cut(s) 1068
AsuC2I CCSGG 3 cut(s) 102, 782, 783
AsuHPI GGTGA 1 cut(s) 894
AsuII TTCGAA 3 cut(s) 841, 1056, 1087
AvaI CYCGRG 2 cut(s) 583, 781
AvaII GGWCC 1 cut(s) 1068
BamHI GGATCC 1 cut(s) 976
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 5 cut(s) 490, 707, 757, 866, 950
BccI CCATC 4 cut(s) 36, 68, 954, 1294
BceAI ACGGC 3 cut(s) 101, 533, 775
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 465
BclI TGATCA 2 cut(s) 859, 1099
BcnI CCSGG 3 cut(s) 102, 782, 783
BcoDI GTCTC 2 cut(s) 537, 1223
BfaI CTAG 3 cut(s) 335, 738, 1355
BfmI CTRYAG 1 cut(s) 1110
BglII AGATCT 1 cut(s) 306
BisI GCNGC 5 cut(s) 504, 696, 771, 855, 939
BlsI GCNGC 5 cut(s) 505, 697, 772, 856, 940
Bme1390I CCNGG 4 cut(s) 102, 465, 782, 783
Bme18I GGWCC 1 cut(s) 1068
BmeT110I CYCGRG 2 cut(s) 583, 781
BmgT120I GGNCC 1 cut(s) 1068
BmiI GGNNCC 2 cut(s) 875, 978
BmrFI CCNGG 4 cut(s) 102, 465, 782, 783
BmrI ACTGGG 2 cut(s) 494, 955
BmuI ACTGGG 2 cut(s) 494, 955
Bpu10I CCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 3 cut(s) 841, 1056, 1087
BpuEI CTTGAG 1 cut(s) 1247
BpuMI CCSGG 3 cut(s) 102, 782, 783
BsaJI CCNNGG 3 cut(s) 100, 781, 1071
BsaWI WCCGGW 2 cut(s) 203, 1217
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 787
Bse118I RCCGGY 2 cut(s) 203, 1217
Bse1I ACTGG 4 cut(s) 171, 489, 901, 950
Bse3DI GCAATG 1 cut(s) 992
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 100, 781, 1071
BseGI GGATG 4 cut(s) 28, 352, 965, 969
BseLI CCNNNNNNNGG 2 cut(s) 44, 787
BseMI GCAATG 1 cut(s) 992
BseMII CTCAG 1 cut(s) 1233
BseNI ACTGG 4 cut(s) 171, 489, 901, 950
BseRI GAGGAG 2 cut(s) 24, 662
BseXI GCAGC 5 cut(s) 490, 707, 757, 866, 950
BseYI CCCAGC 1 cut(s) 1169
Bsh1285I CGRYCG 1 cut(s) 1222
BshFI GGCC 2 cut(s) 170, 210
BshTI ACCGGT 2 cut(s) 203, 1217
BsiEI CGRYCG 1 cut(s) 1222
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 2 cut(s) 583, 781
BsiSI CCGG 5 cut(s) 102, 204, 659, 782, 1218
BslFI GGGAC 3 cut(s) 208, 237, 263
BslI CCNNNNNNNGG 2 cut(s) 44, 787
BsmAI GTCTC 2 cut(s) 537, 1223
BsmFI GGGAC 3 cut(s) 208, 237, 263
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 170, 210
BsoBI CYCGRG 2 cut(s) 583, 781
Bsp119I TTCGAA 3 cut(s) 841, 1056, 1087
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
BspACI CCGC 3 cut(s) 25, 762, 1193
BspANI GGCC 2 cut(s) 170, 210
BspCNI CTCAG 1 cut(s) 1234
BspLI GGNNCC 2 cut(s) 875, 978
BspMAI CTGCAG 1 cut(s) 1114
BspPI GGATC 3 cut(s) 650, 971, 984
BspT104I TTCGAA 3 cut(s) 841, 1056, 1087
BsrDI GCAATG 1 cut(s) 992
BsrFI RCCGGY 2 cut(s) 203, 1217
BsrI ACTGG 4 cut(s) 171, 489, 901, 950
BssAI RCCGGY 2 cut(s) 203, 1217
BssECI CCNNGG 3 cut(s) 100, 781, 1071
BssMI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
BssT1I CCWWGG 1 cut(s) 1071
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 3 cut(s) 299, 552, 1234
BstBI TTCGAA 3 cut(s) 841, 1056, 1087
BstC8I GCNNGC 1 cut(s) 1110
BstDEI CTNAG 6 cut(s) 65, 121, 303, 453, 1242, 1295
BstF5I GGATG 4 cut(s) 28, 352, 965, 969
BstKTI GATC 6 cut(s) 309, 658, 862, 979, 1102, 1248
BstMAI GTCTC 2 cut(s) 537, 1223
BstMBI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
BstMCI CGRYCG 1 cut(s) 1222
BstMWI GCNNNNNNNGC 6 cut(s) 167, 207, 391, 509, 692, 935
BstNI CCWGG 1 cut(s) 465
BstSCI CCNGG 4 cut(s) 100, 463, 780, 781
BstSFI CTRYAG 1 cut(s) 1110
BstV1I GCAGC 5 cut(s) 490, 707, 757, 866, 950
BstX2I RGATCY 3 cut(s) 306, 655, 976
BstYI RGATCY 3 cut(s) 306, 655, 976
BsuRI GGCC 2 cut(s) 170, 210
BtsCI GGATG 4 cut(s) 28, 352, 965, 969
BtsI GCAGTG 2 cut(s) 228, 507
BtsIMutI CAGTG 4 cut(s) 228, 268, 507, 1008
Cac8I GCNNGC 1 cut(s) 1110
CaiI CAGNNNCTG 1 cut(s) 1180
Cfr10I RCCGGY 2 cut(s) 203, 1217
Cfr13I GGNCC 1 cut(s) 1068
Cfr9I CCCGGG 1 cut(s) 781
Csp6I GTAC 1 cut(s) 894
CspAI ACCGGT 2 cut(s) 203, 1217
CviAII CATG 7 cut(s) 223, 343, 682, 749, 1027, 1097, 1117
CviQI GTAC 1 cut(s) 894
DdeI CTNAG 6 cut(s) 65, 121, 303, 453, 1242, 1295
DpnI GATC 6 cut(s) 308, 657, 861, 978, 1101, 1247
DpnII GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 168
EciI GGCGGA 2 cut(s) 14, 777
Eco130I CCWWGG 1 cut(s) 1071
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 1 cut(s) 1068
Eco57I CTGAAG 4 cut(s) 119, 365, 675, 1032
Eco88I CYCGRG 2 cut(s) 583, 781
EcoRI GAATTC 4 cut(s) 403, 743, 1089, 1120
EcoRII CCWGG 1 cut(s) 463
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 1 cut(s) 1071
EcoT22I ATGCAT 1 cut(s) 683
ErhI CCWWGG 1 cut(s) 1071
FaeI CATG 7 cut(s) 226, 346, 685, 752, 1030, 1100, 1120
FaqI GGGAC 3 cut(s) 208, 237, 263
FatI CATG 7 cut(s) 222, 342, 681, 748, 1026, 1096, 1116
FauNDI CATATG 1 cut(s) 677
FbaI TGATCA 2 cut(s) 859, 1099
Fnu4HI GCNGC 5 cut(s) 504, 696, 771, 855, 939
FokI GGATG 4 cut(s) 15, 359, 972, 976
Fsp4HI GCNGC 5 cut(s) 504, 696, 771, 855, 939
FspBI CTAG 3 cut(s) 335, 738, 1355
GluI GCNGC 5 cut(s) 504, 696, 771, 855, 939
GsaI CCCAGC 1 cut(s) 1173
HaeIII GGCC 2 cut(s) 170, 210
HapII CCGG 5 cut(s) 102, 204, 659, 782, 1218
Hin1II CATG 7 cut(s) 226, 346, 685, 752, 1030, 1100, 1120
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HindIII AAGCTT 2 cut(s) 131, 455
HinfI GANTC 5 cut(s) 84, 556, 967, 1058, 1299
HpaII CCGG 5 cut(s) 102, 204, 659, 782, 1218
HphI GGTGA 1 cut(s) 894
Hpy166II GTNNAC 2 cut(s) 757, 902
Hpy188I TCNGA 2 cut(s) 561, 673
Hpy188III TCNNGA 7 cut(s) 137, 236, 290, 583, 820, 833, 1265
Hpy8I GTNNAC 2 cut(s) 757, 902
HpyAV CCTTC 2 cut(s) 524, 1094
HpyCH4III ACNGT 3 cut(s) 299, 552, 1234
HpyCH4V TGCA 8 cut(s) 17, 161, 191, 329, 681, 716, 997, 1112
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 207, 391, 509, 692, 935
HpyF3I CTNAG 6 cut(s) 65, 121, 303, 453, 1242, 1295
Hsp92II CATG 7 cut(s) 226, 346, 685, 752, 1030, 1100, 1120
Ksp22I TGATCA 2 cut(s) 859, 1099
Kzo9I GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
LmnI GCTCC 4 cut(s) 238, 609, 649, 1177
Lsp1109I GCAGC 5 cut(s) 490, 707, 757, 866, 950
MaeI CTAG 3 cut(s) 335, 738, 1355
MaeIII GTNAC 6 cut(s) 214, 448, 513, 805, 1022, 1182
MalI GATC 6 cut(s) 308, 657, 861, 978, 1101, 1247
MboI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
MboII GAAGA 4 cut(s) 139, 452, 610, 1154
MfeI CAATTG 2 cut(s) 471, 1103
MflI RGATCY 3 cut(s) 306, 655, 976
MhlI GDGCHC 1 cut(s) 203
MlyI GAGTC 1 cut(s) 93
MmeI TCCRAC 1 cut(s) 631
MnlI CCTC 7 cut(s) 32, 42, 45, 71, 367, 640, 1052
Mph1103I ATGCAT 1 cut(s) 683
MseI TTAA 2 cut(s) 1236, 1363
MslI CAYNNNNRTG 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 5 cut(s) 102, 204, 659, 782, 1218
MspR9I CCNGG 4 cut(s) 102, 465, 782, 783
MunI CAATTG 2 cut(s) 471, 1103
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 6 cut(s) 167, 207, 391, 509, 692, 935
NciI CCSGG 3 cut(s) 102, 782, 783
NdeI CATATG 1 cut(s) 677
NdeII GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
NlaIII CATG 7 cut(s) 226, 346, 685, 752, 1030, 1100, 1120
NlaIV GGNNCC 2 cut(s) 875, 978
NmuCI GTSAC 3 cut(s) 214, 805, 1022
NsiI ATGCAT 1 cut(s) 683
NspV TTCGAA 3 cut(s) 841, 1056, 1087
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 4 cut(s) 556, 967, 1058, 1299
PflFI GACNNNGTC 1 cut(s) 1028
PinAI ACCGGT 2 cut(s) 203, 1217
PkrI GCNGC 5 cut(s) 505, 697, 772, 856, 940
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 2 cut(s) 875, 978
PspPI GGNCC 1 cut(s) 1068
PstI CTGCAG 1 cut(s) 1114
PstNI CAGNNNCTG 1 cut(s) 1180
PsuI RGATCY 3 cut(s) 306, 655, 976
PsyI GACNNNGTC 1 cut(s) 1028
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 1 cut(s) 895
RsaNI GTAC 1 cut(s) 894
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 2 cut(s) 1236, 1363
SatI GCNGC 5 cut(s) 504, 696, 771, 855, 939
Sau3AI GATC 6 cut(s) 306, 655, 859, 976, 1099, 1245
Sau96I GGNCC 1 cut(s) 1068
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 4 cut(s) 102, 465, 782, 783
SduI GDGCHC 1 cut(s) 203
SfcI CTRYAG 1 cut(s) 1110
SfuI TTCGAA 3 cut(s) 841, 1056, 1087
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 1068
SmaI CCCGGG 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 1226
SmoI CTYRAG 1 cut(s) 1226
SsiI CCGC 3 cut(s) 25, 762, 1193
SspMI CTAG 3 cut(s) 335, 738, 1355
StyD4I CCNGG 4 cut(s) 100, 463, 780, 781
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 3 cut(s) 299, 552, 1234
TaqI TCGA 6 cut(s) 180, 841, 1032, 1056, 1087, 1266
TfiI GAWTC 4 cut(s) 556, 967, 1058, 1299
Tru1I TTAA 2 cut(s) 1236, 1363
Tru9I TTAA 2 cut(s) 1236, 1363
TscAI CASTG 4 cut(s) 235, 268, 514, 1015
TseFI GTSAC 3 cut(s) 214, 805, 1022
TseI GCWGC 5 cut(s) 503, 695, 770, 854, 938
Tsp45I GTSAC 3 cut(s) 214, 805, 1022
TspDTI ATGAA 8 cut(s) 255, 363, 909, 959, 1133, 1220, 1269, 1291
TspMI CCCGGG 1 cut(s) 781
TspRI CASTG 4 cut(s) 235, 268, 514, 1015
Tth111I GACNNNGTC 1 cut(s) 1028
VpaK11BI GGWCC 1 cut(s) 1068
XapI RAATTY 5 cut(s) 403, 722, 743, 1089, 1120
XmaI CCCGGG 1 cut(s) 781
XspI CTAG 3 cut(s) 335, 738, 1355
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.