Rmu_co8293057.1_g000001

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8293057.1
OG0020446
Physical Location & Seq
Forward (+)
1 .. 789
789 bp
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UTR
Exon/CDS
Intron
Rmu_co8293057.1_g000001.1.cds

Sequence Viewer

Length: 561 bp
gatcctaaaacggagaacaatccctaccttggtttcatttatacttcatttcaagagagggctaccttcatctcccatgggaacacagccaggcaagcaaaagagcatggggatgtgaaattggctcagatatgtggcaccattgcctcggatgaaaaacgtcatgaaactgcctatacaaagattgtggagaagctctttgagatagacccagatggcactgtattggctttagctgacatgatgaagaaaaaaataacaatgcctgcccacttgatgtatgatggtcaggattataatcttttcgagaatttctcagctgttgcccagcggctggaagtttatacggccaaggattacgctgatattttggagtttctggttggtagatggaaaatagaggagttgactggtctttctggtgagggtcgtagagcacaagatttcgtatgtggattgccaccgagaattagaaggttggaggagagagctcagggaagagccaagaaatcatccagtgttccattcagctggctttttggtagagagataatcgtttaa

Protein Analysis

186

Amino Acids

21.18

Weight (kDa)

6.5

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020446)

Species Orthologous Gene IDs
pyrus_communis pycom12g24230
rosa_multiflora Rmu_co8293057.1_g000001 Rmu_co8451205.1_g000001 Rmu_sc0016148.1_g000002 Rmu_ssc0000473.1_g000018
rosa_roxburghii Rroxscaffold_2G00120150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 1 cut(s) 331
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 1 cut(s) 310
AfiI CCNNNNNNNGG 2 cut(s) 29, 334
AgsI TTSAA 1 cut(s) 53
AjnI CCWGG 1 cut(s) 89
AluBI AGCT 5 cut(s) 196, 236, 320, 491, 531
AluI AGCT 5 cut(s) 196, 236, 320, 491, 531
Alw21I GWGCWC 2 cut(s) 439, 493
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 1 cut(s) 348
ApoI RAATTY 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 434
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 493
Bbv12I GWGCWC 2 cut(s) 439, 493
BccI CCATC 3 cut(s) 209, 278, 384
BceAI ACGGC 1 cut(s) 363
BciT130I CCWGG 1 cut(s) 91
BisI GCNGC 1 cut(s) 332
BlsI GCNGC 1 cut(s) 333
Bme1390I CCNGG 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 91
BplI GAGNNNNNCTC 2 cut(s) 299, 331
Bpu10I CCTNAGC 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 297
BsaJI CCNNGG 4 cut(s) 28, 76, 147, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 334
Bse1I ACTGG 2 cut(s) 415, 516
Bse3DI GCAATG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 4 cut(s) 28, 76, 147, 351
BseGI GGATG 3 cut(s) 118, 157, 512
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 29, 334
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 3 cut(s) 140, 330, 506
BseNI ACTGG 2 cut(s) 415, 516
BseRI GAGGAG 2 cut(s) 416, 497
BseYI CCCAGC 1 cut(s) 327
BshFI GGCC 1 cut(s) 350
BshNI GGYRCC 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 439, 493
BslI CCNNNNNNNGG 2 cut(s) 29, 334
BsnI GGCC 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 439, 493
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 331
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 3 cut(s) 139, 329, 505
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 141
BsrI ACTGG 2 cut(s) 415, 516
BssECI CCNNGG 4 cut(s) 28, 76, 147, 351
BssT1I CCWWGG 3 cut(s) 28, 76, 351
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 493
BstC8I GCNNGC 3 cut(s) 96, 267, 533
BstDEI CTNAG 3 cut(s) 126, 316, 492
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 3 cut(s) 118, 157, 512
BstKTI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 89
BstXI CCANNNNNNTGG 1 cut(s) 531
BsuRI GGCC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 3 cut(s) 118, 157, 512
BtsIMutI CAGTG 2 cut(s) 219, 523
Cac8I GCNNGC 3 cut(s) 96, 267, 533
CaiI CAGNNNCTG 1 cut(s) 334
CciI TCATGA 1 cut(s) 163
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviAII CATG 4 cut(s) 77, 107, 164, 241
DdeI CTNAG 3 cut(s) 126, 316, 492
DpnI GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
Ecl136II GAGCTC 1 cut(s) 491
Eco130I CCWWGG 3 cut(s) 28, 76, 351
Eco24I GRGCYC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 3 cut(s) 28, 76, 351
EcoT38I GRGCYC 1 cut(s) 493
ErhI CCWWGG 3 cut(s) 28, 76, 351
FaeI CATG 4 cut(s) 80, 110, 167, 244
FatI CATG 4 cut(s) 76, 106, 163, 240
Fnu4HI GCNGC 1 cut(s) 332
FokI GGATG 3 cut(s) 125, 164, 499
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 1 cut(s) 332
GluI GCNGC 1 cut(s) 332
GsaI CCCAGC 1 cut(s) 331
HaeIII GGCC 1 cut(s) 350
Hin1II CATG 4 cut(s) 80, 110, 167, 244
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 2 cut(s) 129, 151
Hpy188III TCNNGA 4 cut(s) 53, 164, 290, 307
Hpy8I GTNNAC 1 cut(s) 408
HpyAV CCTTC 2 cut(s) 76, 468
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpyF3I CTNAG 3 cut(s) 126, 316, 492
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 4 cut(s) 80, 110, 167, 244
LguI GCTCTTC 1 cut(s) 493
MaeII ACGT 1 cut(s) 160
MalI GATC 1 cut(s) 3
MboII GAAGA 2 cut(s) 259, 510
MhlI GDGCHC 2 cut(s) 439, 493
MluCI AATT 3 cut(s) 119, 310, 468
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 5 cut(s) 51, 157, 394, 418, 475
MseI TTAA 1 cut(s) 559
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 3 cut(s) 320, 331, 531
MspR9I CCNGG 1 cut(s) 91
MvaI CCWGG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 95
NcoI CCATGG 1 cut(s) 76
NlaIII CATG 4 cut(s) 80, 110, 167, 244
NlaIV GGNNCC 1 cut(s) 139
PagI TCATGA 1 cut(s) 163
PciSI GCTCTTC 1 cut(s) 493
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 1 cut(s) 333
PsiI TTATAA 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 1 cut(s) 139
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstNI CAGNNNCTG 1 cut(s) 334
PvuII CAGCTG 2 cut(s) 320, 531
RseI CAYNNNNRTG 1 cut(s) 111
SacI GAGCTC 1 cut(s) 493
SapI GCTCTTC 1 cut(s) 493
SaqAI TTAA 1 cut(s) 559
SatI GCNGC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 2 cut(s) 439, 493
SetI ASST 9 cut(s) 30, 68, 163, 198, 238, 322, 479, 493, 533
SmiMI CAYNNNNRTG 1 cut(s) 111
Sse9I AATT 3 cut(s) 119, 310, 468
SsiI CCGC 1 cut(s) 331
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 3 cut(s) 28, 76, 351
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 306
TasI AATT 3 cut(s) 119, 310, 468
TauI GCSGC 1 cut(s) 334
Tru1I TTAA 1 cut(s) 559
Tru9I TTAA 1 cut(s) 559
TscAI CASTG 2 cut(s) 226, 523
TspDTI ATGAA 6 cut(s) 25, 36, 58, 168, 180, 260
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 2 cut(s) 226, 523
Van91I CCANNNNNTGG 1 cut(s) 334
XapI RAATTY 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.