Rmu_co8451205.1_g000001

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8451205.1
OG0020446
Physical Location & Seq
Reverse (-)
646 .. 1587
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8451205.1_g000001.1.cds

Sequence Viewer

Length: 573 bp
atgtcggagccaggaacggagaacaacccgtacttgggtttcgtgtacgcgtcatttcaagagcgagccacgttcatatctcacggcaacacggctcggctggctaaggactgtggtgatccggtgcttgcccgcatatgcggcaccattgcatccgatgagaggcgccacgagtacgcctattccaagattgtagaaaagctatttgaagtggacccgactacagcaatggttgccattgcagacatgatgaggaagaagatcataatgccgggtcatctcatgtatgatggggaggaccccaagctatacgagcactttggggccgtggctcaacggcttggggtgtacacaacacatgattatgcggatattttggagttttttatcggacggtggaggctggagaagatggagggattgacgggagagggtaagcgtgcacaagactttgtgtgtgggttgttggtgaggatcaggaggctagaagagtgggccgatgagcgagcaagaaagatgaagcctcatggcgtcaagtttagctggatttataataaagaagtggtgctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

190

Amino Acids

21.86

Weight (kDa)

6.6

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020446)

Species Orthologous Gene IDs
pyrus_communis pycom12g24230
rosa_multiflora Rmu_co8293057.1_g000001 Rmu_co8451205.1_g000001 Rmu_sc0016148.1_g000002 Rmu_ssc0000473.1_g000018
rosa_roxburghii Rroxscaffold_2G00120150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccB1I GGYRCC 2 cut(s) 143, 165
AccII CGCG 1 cut(s) 50
AciI CCGC 3 cut(s) 133, 141, 368
AclWI GGATC 2 cut(s) 113, 482
AcyI GRCGYC 2 cut(s) 166, 531
AfaI GTAC 4 cut(s) 32, 47, 176, 350
AfiI CCNNNNNNNGG 3 cut(s) 34, 35, 162
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 2 cut(s) 59, 209
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 3 cut(s) 202, 307, 543
AluI AGCT 3 cut(s) 202, 307, 543
Alw21I GWGCWC 2 cut(s) 318, 445
Alw44I GTGCAC 1 cut(s) 441
AlwI GGATC 2 cut(s) 113, 482
AoxI GGCC 2 cut(s) 324, 495
ApaLI GTGCAC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 4 cut(s) 214, 298, 324, 495
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 128, 481
AvaII GGWCC 2 cut(s) 214, 298
BaeGI GKGCMC 1 cut(s) 445
BanI GGYRCC 2 cut(s) 143, 165
BauI CACGAG 1 cut(s) 170
Bbv12I GWGCWC 2 cut(s) 318, 445
BccI CCATC 2 cut(s) 284, 406
BceAI ACGGC 4 cut(s) 100, 108, 311, 353
BciT130I CCWGG 1 cut(s) 12
BcnI CCSGG 1 cut(s) 273
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 169
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
Bme1390I CCNGG 2 cut(s) 12, 273
Bme18I GGWCC 2 cut(s) 214, 298
BmgT120I GGNCC 4 cut(s) 214, 298, 324, 495
BmiI GGNNCC 6 cut(s) 9, 145, 167, 216, 300, 325
BmrFI CCNGG 2 cut(s) 12, 273
BmsI GCATC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 425
Bpu10I CCTNAGC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 273
BsaHI GRCGYC 2 cut(s) 166, 531
BsaJI CCNNGG 1 cut(s) 327
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 35, 162
Bse3DI GCAATG 3 cut(s) 147, 234, 237
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 1 cut(s) 152
BseLI CCNNNNNNNGG 3 cut(s) 34, 35, 162
BseMI GCAATG 3 cut(s) 147, 234, 237
BseSI GKGCMC 1 cut(s) 445
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 326, 497
BshNI GGYRCC 2 cut(s) 143, 165
BsiHKAI GWGCWC 2 cut(s) 318, 445
BsiSI CCGG 2 cut(s) 122, 272
BslI CCNNNNNNNGG 3 cut(s) 34, 35, 162
BsnI GGCC 2 cut(s) 326, 497
Bsp1286I GDGCHC 2 cut(s) 318, 445
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 3 cut(s) 118, 261, 474
BspACI CCGC 3 cut(s) 133, 141, 368
BspANI GGCC 2 cut(s) 326, 497
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 6 cut(s) 9, 145, 167, 216, 300, 325
BspPI GGATC 2 cut(s) 113, 482
BspT107I GGYRCC 2 cut(s) 143, 165
BsrDI GCAATG 3 cut(s) 147, 234, 237
BsrGI TGTACA 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 3 cut(s) 118, 261, 474
BssNI GRCGYC 2 cut(s) 166, 531
BssSI CACGAG 1 cut(s) 170
Bst2BI CACGAG 1 cut(s) 170
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 2 cut(s) 113, 396
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 2 cut(s) 166, 531
BstAPI GCANNNNNTGC 1 cut(s) 233
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 6 cut(s) 66, 102, 129, 133, 441, 507
BstDEI CTNAG 1 cut(s) 105
BstDSI CCRYGG 1 cut(s) 327
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 3 cut(s) 121, 264, 477
BstMBI GATC 3 cut(s) 118, 261, 474
BstMWI GCNNNNNNNGC 4 cut(s) 101, 141, 233, 313
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 2 cut(s) 10, 271
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 445
BstUI CGCG 1 cut(s) 50
BsuRI GGCC 2 cut(s) 326, 497
BtgI CCRYGG 1 cut(s) 327
BtsCI GGATG 1 cut(s) 152
Cac8I GCNNGC 6 cut(s) 66, 102, 129, 133, 441, 507
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 4 cut(s) 214, 298, 324, 495
CseI GACGC 2 cut(s) 39, 520
Csp6I GTAC 4 cut(s) 31, 46, 175, 349
CviAII CATG 4 cut(s) 247, 283, 359, 527
CviQI GTAC 4 cut(s) 31, 46, 175, 349
DdeI CTNAG 1 cut(s) 105
DinI GGCGCC 1 cut(s) 167
DpnI GATC 3 cut(s) 120, 263, 476
DpnII GATC 3 cut(s) 118, 261, 474
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 214, 298
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 10
EgeI GGCGCC 1 cut(s) 167
EheI GGCGCC 1 cut(s) 167
FaeI CATG 4 cut(s) 250, 286, 362, 530
FatI CATG 4 cut(s) 246, 282, 358, 526
FauI CCCGC 1 cut(s) 140
FauNDI CATATG 1 cut(s) 137
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 1 cut(s) 485
GlaI GCGC 1 cut(s) 167
GluI GCNGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 326, 497
HapII CCGG 2 cut(s) 122, 272
HgaI GACGC 2 cut(s) 39, 520
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 2 cut(s) 166, 531
Hin1II CATG 4 cut(s) 250, 286, 362, 530
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HpaII CCGG 2 cut(s) 122, 272
HphI GGTGA 2 cut(s) 128, 481
Hpy166II GTNNAC 5 cut(s) 46, 214, 349, 351, 443
Hpy188I TCNGA 3 cut(s) 7, 157, 392
Hpy188III TCNNGA 2 cut(s) 59, 478
Hpy8I GTNNAC 5 cut(s) 46, 214, 349, 351, 443
HpyCH4III ACNGT 2 cut(s) 113, 396
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 3 cut(s) 152, 242, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 141, 233, 313
HpyF3I CTNAG 1 cut(s) 105
HpySE526I ACGT 1 cut(s) 71
Hsp92I GRCGYC 2 cut(s) 166, 531
Hsp92II CATG 4 cut(s) 250, 286, 362, 530
HspAI GCGC 1 cut(s) 166
KasI GGCGCC 1 cut(s) 165
Kzo9I GATC 3 cut(s) 118, 261, 474
LmnI GCTCC 1 cut(s) 7
LpnPI CCDG 7 cut(s) 24, 86, 135, 285, 389, 463, 529
LweI GCATC 1 cut(s) 161
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 71
MalI GATC 3 cut(s) 120, 263, 476
MboI GATC 3 cut(s) 118, 261, 474
MboII GAAGA 4 cut(s) 268, 271, 421, 500
MhlI GDGCHC 2 cut(s) 318, 445
MluI ACGCGT 1 cut(s) 48
Mly113I GGCGCC 1 cut(s) 166
MnlI CCTC 9 cut(s) 156, 246, 289, 393, 409, 424, 465, 474, 534
MslI CAYNNNNRTG 1 cut(s) 363
MspI CCGG 2 cut(s) 122, 272
MspR9I CCNGG 2 cut(s) 12, 273
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 4 cut(s) 101, 141, 233, 313
NarI GGCGCC 1 cut(s) 166
NciI CCSGG 1 cut(s) 273
NdeI CATATG 1 cut(s) 137
NdeII GATC 3 cut(s) 118, 261, 474
NlaIII CATG 4 cut(s) 250, 286, 362, 530
NlaIV GGNNCC 6 cut(s) 9, 145, 167, 216, 300, 325
NmeAIII GCCGAG 1 cut(s) 76
PkrI GCNGC 1 cut(s) 143
PluTI GGCGCC 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 298
PsiI TTATAA 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 6 cut(s) 9, 145, 167, 216, 300, 325
PspPI GGNCC 4 cut(s) 214, 298, 324, 495
PspPPI RGGWCCY 1 cut(s) 298
PsrI GAACNNNNNNTAC 2 cut(s) 14, 46
RsaI GTAC 4 cut(s) 32, 47, 176, 350
RsaNI GTAC 4 cut(s) 31, 46, 175, 349
RseI CAYNNNNRTG 1 cut(s) 363
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 3 cut(s) 118, 261, 474
Sau96I GGNCC 4 cut(s) 214, 298, 324, 495
ScrFI CCNGG 2 cut(s) 12, 273
SduI GDGCHC 2 cut(s) 318, 445
SetI ASST 4 cut(s) 74, 204, 309, 545
SfaNI GCATC 1 cut(s) 161
SfcI CTRYAG 1 cut(s) 222
SfoI GGCGCC 1 cut(s) 167
SinI GGWCC 2 cut(s) 214, 298
SmiMI CAYNNNNRTG 1 cut(s) 363
SsiI CCGC 3 cut(s) 133, 141, 368
SspDI GGCGCC 1 cut(s) 165
SspMI CTAG 1 cut(s) 485
StyD4I CCNGG 2 cut(s) 10, 271
TaaI ACNGT 2 cut(s) 113, 396
TaiI ACGT 1 cut(s) 74
TatI WGTACW 1 cut(s) 348
TauI GCSGC 1 cut(s) 144
TspDTI ATGAA 2 cut(s) 64, 533
TspGWI ACGGA 1 cut(s) 32
VneI GTGCAC 1 cut(s) 441
VpaK11BI GGWCC 2 cut(s) 214, 298
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.