Rmu_sc0016148.1_g000002

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016148.1
OG0020446
Physical Location & Seq
Reverse (-)
2801 .. 3145
345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016148.1_g000002.1.cds

Sequence Viewer

Length: 345 bp
atggtcgccattgcggacatgatgaggaagaagatcacaatgccggcccatcttatgtacgatggggaggatcccaagctgttcgagcattttgcggccgtggctcaacggcttagggtgtacacggcacatgattatgcggacattttggagtttttgatcggacggtggaggctggagaaggtggaaggaatgacgggggagggtaagcgtgcacaggacttcgtgtgtgggttggcgccgaggatcaggaggctagaagagcgggccgatgagcgagcaaggaagatggagccgcatggcgtcaagtttagctggatttataataaagaagtggtgctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

114

Amino Acids

13.37

Weight (kDa)

8.76

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020446)

Species Orthologous Gene IDs
pyrus_communis pycom12g24230
rosa_multiflora Rmu_co8293057.1_g000001 Rmu_co8451205.1_g000001 Rmu_sc0016148.1_g000002 Rmu_ssc0000473.1_g000018
rosa_roxburghii Rroxscaffold_2G00120150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 265
AciI CCGC 5 cut(s) 14, 95, 140, 265, 296
AclWI GGATC 3 cut(s) 65, 78, 254
AcoI YGGCCR 1 cut(s) 96
AcyI GRCGYC 2 cut(s) 239, 303
AfaI GTAC 2 cut(s) 59, 122
AluBI AGCT 2 cut(s) 79, 315
AluI AGCT 2 cut(s) 79, 315
Alw21I GWGCWC 1 cut(s) 217
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 3 cut(s) 65, 78, 254
AoxI GGCC 3 cut(s) 45, 96, 267
ApaLI GTGCAC 1 cut(s) 213
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 2 cut(s) 46, 267
BaeGI GKGCMC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 217
BccI CCATC 3 cut(s) 56, 57, 283
BceAI ACGGC 3 cut(s) 83, 125, 141
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BfaI CTAG 1 cut(s) 257
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 2 cut(s) 96, 296
BlsI GCNGC 2 cut(s) 97, 297
BmgT120I GGNCC 2 cut(s) 46, 267
BmiI GGNNCC 3 cut(s) 72, 240, 294
BpmI CTGGAG 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 113
BsaHI GRCGYC 2 cut(s) 239, 303
BsaJI CCNNGG 2 cut(s) 99, 242
Bse118I RCCGGY 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 99, 242
BseMI GCAATG 1 cut(s) 9
BseSI GKGCMC 1 cut(s) 217
BseX3I CGGCCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 47, 98, 269
BshNI GGYRCC 1 cut(s) 238
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 44
BsnI GGCC 3 cut(s) 47, 98, 269
Bsp1286I GDGCHC 1 cut(s) 217
Bsp1407I TGTACA 1 cut(s) 120
Bsp143I GATC 4 cut(s) 33, 70, 159, 246
BspACI CCGC 5 cut(s) 14, 95, 140, 265, 296
BspANI GGCC 3 cut(s) 47, 98, 269
BspLI GGNNCC 3 cut(s) 72, 240, 294
BspPI GGATC 3 cut(s) 65, 78, 254
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 238
BsrBI CCGCTC 1 cut(s) 265
BsrDI GCAATG 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 43
BsrGI TGTACA 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 99, 242
BssMI GATC 4 cut(s) 33, 70, 159, 246
BssNI GRCGYC 2 cut(s) 239, 303
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 1 cut(s) 255
BstACI GRCGYC 2 cut(s) 239, 303
BstAUI TGTACA 1 cut(s) 120
BstC8I GCNNGC 4 cut(s) 45, 213, 267, 279
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 4 cut(s) 36, 73, 162, 249
BstMBI GATC 4 cut(s) 33, 70, 159, 246
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 3 cut(s) 85, 101, 262
BstSLI GKGCMC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 3 cut(s) 47, 98, 269
BtgI CCRYGG 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 45, 213, 267, 279
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 46, 267
CseI GACGC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 58, 121
CviAII CATG 3 cut(s) 19, 131, 299
CviQI GTAC 2 cut(s) 58, 121
DdeI CTNAG 1 cut(s) 113
DinI GGCGCC 1 cut(s) 240
DpnI GATC 4 cut(s) 35, 72, 161, 248
DpnII GATC 4 cut(s) 33, 70, 159, 246
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EclXI CGGCCG 1 cut(s) 96
Eco52I CGGCCG 1 cut(s) 96
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
FaeI CATG 3 cut(s) 22, 134, 302
FaiI YATR 6 cut(s) 20, 56, 132, 138, 300, 324
FatI CATG 3 cut(s) 18, 130, 298
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 96, 296
Fsp4HI GCNGC 2 cut(s) 96, 296
FspBI CTAG 1 cut(s) 257
GlaI GCGC 1 cut(s) 240
GluI GCNGC 2 cut(s) 96, 296
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 3 cut(s) 47, 98, 269
HapII CCGG 1 cut(s) 44
HgaI GACGC 1 cut(s) 292
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 2 cut(s) 239, 303
Hin1II CATG 3 cut(s) 22, 134, 302
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HpaII CCGG 1 cut(s) 44
Hpy166II GTNNAC 3 cut(s) 121, 123, 215
Hpy188I TCNGA 1 cut(s) 164
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 3 cut(s) 121, 123, 215
HpyAV CCTTC 2 cut(s) 175, 182
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4V TGCA 1 cut(s) 215
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 101, 262
HpyF3I CTNAG 1 cut(s) 113
Hsp92I GRCGYC 2 cut(s) 239, 303
Hsp92II CATG 3 cut(s) 22, 134, 302
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
KroI GCCGGC 1 cut(s) 43
KroNI GCCGGC 1 cut(s) 45
Kzo9I GATC 4 cut(s) 33, 70, 159, 246
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 5 cut(s) 57, 161, 203, 235, 301
MaeI CTAG 1 cut(s) 257
MalI GATC 4 cut(s) 35, 72, 161, 248
MbiI CCGCTC 1 cut(s) 265
MboI GATC 4 cut(s) 33, 70, 159, 246
MboII GAAGA 4 cut(s) 40, 43, 272, 298
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 217
Mly113I GGCGCC 1 cut(s) 239
MnlI CCTC 6 cut(s) 18, 61, 165, 196, 237, 246
MroNI GCCGGC 1 cut(s) 43
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 1 cut(s) 44
MwoI GCNNNNNNNGC 3 cut(s) 85, 101, 262
NaeI GCCGGC 1 cut(s) 45
NarI GGCGCC 1 cut(s) 239
NdeII GATC 4 cut(s) 33, 70, 159, 246
NgoMIV GCCGGC 1 cut(s) 43
NlaIII CATG 3 cut(s) 22, 134, 302
NlaIV GGNNCC 3 cut(s) 72, 240, 294
NmeAIII GCCGAG 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 45
PkrI GCNGC 2 cut(s) 97, 297
PluTI GGCGCC 1 cut(s) 242
PsiI TTATAA 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 72, 240, 294
PspPI GGNCC 2 cut(s) 46, 267
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 2 cut(s) 59, 122
RsaNI GTAC 2 cut(s) 58, 121
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 255
SatI GCNGC 2 cut(s) 96, 296
Sau3AI GATC 4 cut(s) 33, 70, 159, 246
Sau96I GGNCC 2 cut(s) 46, 267
SduI GDGCHC 1 cut(s) 217
SetI ASST 3 cut(s) 81, 186, 317
SfoI GGCGCC 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 135
SsiI CCGC 5 cut(s) 14, 95, 140, 265, 296
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 1 cut(s) 257
TaaI ACNGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 84
TatI WGTACW 1 cut(s) 120
TauI GCSGC 2 cut(s) 98, 298
VneI GTGCAC 1 cut(s) 213
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.