Rroxscaffold_2G00120150

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020446
Physical Location & Seq
Reverse (-)
52253341 .. 52256173
2833 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00120150.1

Sequence Viewer

Length: 345 bp
ATGGTTGCCATTGCAGACATGATGAGGAAGAAGATCATAATGCCGGGTCATCTCATGTCTGATGGGGAGGACCCCAAGTTGTACGAGCACTTTGGGGCCGTGGCTCAACGGCTTGGGGTGTACACAACACATGATTATGCGGATATTTTGGAGTTTTTTATCGGACGGTGGAGGCTGGAGAAGATGGAGGGATTGACGGAAGAGGGTAAGCGTGCACAAGACTTTGTGTGCGGGTTGTTGGTGAGGATCCGGAGGCTAGAAGAGCTGGCCGATGAGCGAGCAAGGAAGATGAAGCCGCATGGCGTCAAGTTTAGCTGGATTTATAATAAAGAAGTGGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

114

Amino Acids

13.34

Weight (kDa)

7.88

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 1 - 108 6.9e-39 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020446)

Species Orthologous Gene IDs
pyrus_communis pycom12g24230
rosa_multiflora Rmu_co8293057.1_g000001 Rmu_co8451205.1_g000001 Rmu_sc0016148.1_g000002 Rmu_ssc0000473.1_g000018
rosa_roxburghii Rroxscaffold_2G00120150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 324
AccIII TCCGGA 1 cut(s) 249
AciI CCGC 3 cut(s) 140, 231, 296
AclWI GGATC 2 cut(s) 241, 254
AcoI YGGCCR 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 83, 122
AluBI AGCT 2 cut(s) 265, 315
AluI AGCT 2 cut(s) 265, 315
Alw21I GWGCWC 2 cut(s) 90, 217
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 2 cut(s) 241, 254
Aor13HI TCCGGA 1 cut(s) 249
AoxI GGCC 2 cut(s) 96, 267
ApaLI GTGCAC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 70, 96
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 253
AvaII GGWCC 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 246
Bbv12I GWGCWC 2 cut(s) 90, 217
BccI CCATC 2 cut(s) 56, 178
BceAI ACGGC 2 cut(s) 83, 125
BcnI CCSGG 1 cut(s) 45
BfaI CTAG 1 cut(s) 257
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 70, 96
BmiI GGNNCC 3 cut(s) 72, 97, 248
BmrFI CCNGG 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 45
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 9
BseAI TCCGGA 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 99
BseMI GCAATG 1 cut(s) 9
BseSI GKGCMC 1 cut(s) 217
BshFI GGCC 2 cut(s) 98, 269
BsiHKAI GWGCWC 2 cut(s) 90, 217
BsiSI CCGG 2 cut(s) 44, 250
BsnI GGCC 2 cut(s) 98, 269
Bsp1286I GDGCHC 2 cut(s) 90, 217
Bsp13I TCCGGA 1 cut(s) 249
Bsp1407I TGTACA 1 cut(s) 120
Bsp143I GATC 2 cut(s) 33, 246
BspACI CCGC 3 cut(s) 140, 231, 296
BspANI GGCC 2 cut(s) 98, 269
BspEI TCCGGA 1 cut(s) 249
BspLI GGNNCC 3 cut(s) 72, 97, 248
BspPI GGATC 2 cut(s) 241, 254
BspQI GCTCTTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 9
BsrGI TGTACA 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 33, 246
BssNI GRCGYC 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 2 cut(s) 195, 255
BstACI GRCGYC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 213, 267, 279
BstDSI CCRYGG 1 cut(s) 99
BstKTI GATC 2 cut(s) 36, 249
BstMBI GATC 2 cut(s) 33, 246
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 2 cut(s) 98, 269
BtgI CCRYGG 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 213, 267, 279
Cfr13I GGNCC 2 cut(s) 70, 96
CseI GACGC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 82, 121
CviAII CATG 4 cut(s) 19, 55, 131, 299
CviJI RGCY 9 cut(s) 98, 104, 112, 175, 256, 265, 269, 295, 315
CviKI_1 RGCY 9 cut(s) 98, 104, 112, 175, 256, 265, 269, 295, 315
CviQI GTAC 2 cut(s) 82, 121
DpnI GATC 2 cut(s) 35, 248
DpnII GATC 2 cut(s) 33, 246
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 195, 255
EarI CTCTTC 2 cut(s) 195, 255
Eco47I GGWCC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 70
FaeI CATG 4 cut(s) 22, 58, 134, 302
FaiI YATR 7 cut(s) 20, 38, 56, 132, 138, 300, 324
FatI CATG 4 cut(s) 18, 54, 130, 298
FauI CCCGC 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 1 cut(s) 257
GluI GCNGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 197
HaeIII GGCC 2 cut(s) 98, 269
HapII CCGG 2 cut(s) 44, 250
HgaI GACGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 4 cut(s) 22, 58, 134, 302
HpaII CCGG 2 cut(s) 44, 250
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 3 cut(s) 121, 123, 215
Hpy188I TCNGA 2 cut(s) 61, 164
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 3 cut(s) 121, 123, 215
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 14, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 4 cut(s) 22, 58, 134, 302
Kpn2I TCCGGA 1 cut(s) 249
Kzo9I GATC 2 cut(s) 33, 246
LguI GCTCTTC 1 cut(s) 255
LpnPI CCDG 5 cut(s) 57, 161, 251, 263, 301
MaeI CTAG 1 cut(s) 257
MalI GATC 2 cut(s) 35, 248
MboI GATC 2 cut(s) 33, 246
MboII GAAGA 6 cut(s) 40, 43, 193, 212, 272, 298
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 2 cut(s) 90, 217
MnlI CCTC 7 cut(s) 18, 61, 165, 181, 196, 237, 246
MroI TCCGGA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 2 cut(s) 44, 250
MspR9I CCNGG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 45
NdeII GATC 2 cut(s) 33, 246
NlaIII CATG 4 cut(s) 22, 58, 134, 302
NlaIV GGNNCC 3 cut(s) 72, 97, 248
PciSI GCTCTTC 1 cut(s) 255
PkrI GCNGC 1 cut(s) 297
PpuMI RGGWCCY 1 cut(s) 70
PsiI TTATAA 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 70
PspN4I GGNNCC 3 cut(s) 72, 97, 248
PspPI GGNCC 2 cut(s) 70, 96
PspPPI RGGWCCY 1 cut(s) 70
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 2 cut(s) 83, 122
RsaNI GTAC 2 cut(s) 82, 121
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 255
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 2 cut(s) 33, 246
Sau96I GGNCC 2 cut(s) 70, 96
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 2 cut(s) 90, 217
SetI ASST 2 cut(s) 267, 317
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 135
SsiI CCGC 3 cut(s) 140, 231, 296
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 43
TaaI ACNGT 1 cut(s) 168
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 298
TspDTI ATGAA 1 cut(s) 305
TspGWI ACGGA 1 cut(s) 212
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 70
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.