Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_co8518493.1

Orthogroup ID

OG0010966

Physical Location & Seq

Genomic Coordinates Reverse (-)

462 .. 5130

Gene Structure 4669 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_co8518493.1_g000001.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

atgctgctggtttctggtattacaacaatattgcactcttactttggaacccgacttccattagttcaaggaagttcatttgtgtatttggccccagcgttagttatcatgaatgctcaagaatatcggaatcttacagaacataaatttaggcatataatgagagaactgcaaggagctataattattggttcgatatttcaaagcatcctgggatttactggtttaatgtctttattgctgagattgataaaccctattgtcgttgcaccaactgttgctgcagtaggtttggccttttttagttatggctttcctcaagctggtagttgtgtggaaatcagcattccacagatacttttggttcttatgttcaccttgtaccttcgaggggtaaacatctttgggcatcgtcttttccggatttatgcggttcccttgagcataatgatcatatggacttatgcattctttttgacagccggtggagcatatgattacaaaggctgtacctctgatatacctagttcaaatatcttaatagatgcttgtagaagacatgcatataccatgcaacattgtaggaccgatgtttcaaatgcatggagaactgccgcatgggtcaggattccctaccctctgcagtggggtatccctatcttccatttcaggacttccattatcatggtctttgtatcacttgttgcatcagttgattcggttggaacatatcactctgcatctatgcaaattaatctgaaacctccaacccgaggaattgtcagcagaggaattgcattggagggattctgcagtatattggcaggactttggggttcaggtactgggtcaacaaccttgacagaaaatgttcatacaatcaacataacaaaggtggcaagtcgaagagttgtggaacttggagcagttttcttaatcgtcttctcattcataggaaaagtgggtgccattcttgcctctataccactggccttggctgcttccgtactctgcttcacatgggcccttgttgtggcattgggtctctcaacgttgcagtatagtcgagcagcaagttttagaaacatgatgatagttggtgtttcattgtttcttggtttatctattcctgcatattttcaacagtatcaaccagaaacgagcttcatactccctagctacttcattccatatgcagcagcatctaatggaccggcccgcaccgggagtaaacaacttgactttgctataaatgcacttatgtctttgaacatggtggtgacactcgtggtagcattggtactggaaaacactgtcccaggcagccgtcaggaacgaggggtgtatatatggtcaaaaccagaagacataaataccgatccatcttcacttgaagattattccttaccaagaaaagtttccagatgtctttgccgctctaattgtctgggcgtctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Xan_ur_permease

Protein Analysis

485

Amino Acids

53.14

Weight (kDa)

9.41

Isoelectric Point (pI)

36.76

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010966)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38050
fragaria_vesca	FvH4_1g19590 FvH4_1g19590 FvH4_1g19590
malus_domestica	MD15G1311400.v1.1
prunus_persica	Prupe.6G202400_v2.0.a1 Prupe.6G202400_v2.0.a1 Prupe.6G202400_v2.0.a1 Prupe.6G202400_v2.0.a1
pyrus_communis	pycom15g27520
rosa_chinensis	RchiOBHm_Chr2g0111121
rosa_laevigata	RLG00000017866
rosa_multiflora	Rmu_co8518493.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132690
rosa_rugosa	Rorug02G0171000
rosa_samantha	Rh2AG224000 Rh2BG235600 Rh2CG227100 Rh2DG231600
rosa_wichuraiana	Rw2G017280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	965
AccBSI	CCGCTC	1 cut(s)	1437
AccIII	TCCGGA	1 cut(s)	420
AciI	CCGC	4 cut(s)	431, 615, 1219, 1435
AclI	AACGTT	1 cut(s)	1052
AclWI	GGATC	1 cut(s)	1373
AcsI	RAATTY	1 cut(s)	146
AcyI	GRCGYC	1 cut(s)	1452
AfaI	GTAC	5 cut(s)	383, 511, 844, 1008, 1302
AfiI	CCNNNNNNNGG	5 cut(s)	323, 391, 773, 1033, 1224
AgsI	TTSAA	7 cut(s)	68, 203, 531, 597, 1142, 1270, 1394
AjnI	CCWGG	2 cut(s)	210, 1318
AjuI	GAANNNNNNNTTGG	2 cut(s)	1402, 1434
AloI	GAACNNNNNNTCC	2 cut(s)	40, 72
AluBI	AGCT	4 cut(s)	179, 323, 1164, 1179
AluI	AGCT	4 cut(s)	179, 323, 1164, 1179
Alw26I	GTCTC	1 cut(s)	1049
AlwI	GGATC	1 cut(s)	1373
AlwNI	CAGNNNCTG	1 cut(s)	845
Ama87I	CYCGRG	1 cut(s)	771
Aor13HI	TCCGGA	1 cut(s)	420
AoxI	GGCC	5 cut(s)	90, 294, 990, 1023, 1215
ApaI	GGGCCC	1 cut(s)	1027
ApeKI	GCWGC	7 cut(s)	4, 281, 998, 1070, 1196, 1199, 1323
ApoI	RAATTY	1 cut(s)	146
AseI	ATTAAT	1 cut(s)	753
Asp700I	GAANNNNTTC	2 cut(s)	870, 1417
AspS9I	GGNCC	6 cut(s)	91, 585, 1023, 1024, 1211, 1216
AsuC2I	CCSGG	1 cut(s)	1225
AsuHPI	GGTGA	2 cut(s)	367, 1291
AvaI	CYCGRG	1 cut(s)	771
AvaII	GGWCC	2 cut(s)	585, 1211
BaeGI	GKGCMC	1 cut(s)	1027
BanI	GGYRCC	1 cut(s)	965
BanII	GRGCYC	1 cut(s)	1027
BauI	CACGAG	1 cut(s)	1286
BbsI	GAAGAC	3 cut(s)	562, 934, 1371
BbvI	GCAGC	6 cut(s)	268, 985, 1082, 1208, 1211, 1335
BccI	CCATC	1 cut(s)	1390
BceAI	ACGGC	1 cut(s)	1311
BcgI	CGANNNNNNTGC	2 cut(s)	1046, 1080
BciT130I	CCWGG	2 cut(s)	212, 1320
BciVI	GTATCC	1 cut(s)	662
BclI	TGATCA	1 cut(s)	450
BcnI	CCSGG	1 cut(s)	1225
BcoDI	GTCTC	1 cut(s)	1049
BfaI	CTAG	2 cut(s)	525, 1176
BfmI	CTRYAG	3 cut(s)	282, 641, 811
BfuI	GTATCC	1 cut(s)	662
BisI	GCNGC	9 cut(s)	5, 282, 615, 999, 1071, 1197, 1200, 1324, 1435
BlsI	GCNGC	9 cut(s)	6, 283, 616, 1000, 1072, 1198, 1201, 1325, 1436
Bme1390I	CCNGG	3 cut(s)	212, 1225, 1320
Bme18I	GGWCC	2 cut(s)	585, 1211
BmeT110I	CYCGRG	1 cut(s)	771
BmgT120I	GGNCC	6 cut(s)	91, 585, 1023, 1024, 1211, 1216
BmiI	GGNNCC	5 cut(s)	49, 93, 435, 967, 1025
BmrFI	CCNGG	3 cut(s)	212, 1225, 1320
BmrI	ACTGGG	1 cut(s)	855
BmsI	GCATC	6 cut(s)	216, 418, 535, 716, 749, 1211
BmuI	ACTGGG	1 cut(s)	855
BpiI	GAAGAC	3 cut(s)	562, 934, 1371
BpuEI	CTTGAG	3 cut(s)	102, 303, 460
BpuMI	CCSGG	1 cut(s)	1225
BsaHI	GRCGYC	1 cut(s)	1452
BsaI	GGTCTC	1 cut(s)	1049
BsaJI	CCNNGG	4 cut(s)	211, 772, 993, 1318
BsaWI	WCCGGW	1 cut(s)	420
Bsc4I	CCNNNNNNNGG	5 cut(s)	323, 391, 773, 1033, 1224
Bse118I	RCCGGY	2 cut(s)	482, 1213
Bse1I	ACTGG	4 cut(s)	226, 850, 993, 1308
BseAI	TCCGGA	1 cut(s)	420
BseBI	CCWGG	2 cut(s)	212, 1320
BseDI	CCNNGG	4 cut(s)	211, 772, 993, 1318
BseGI	GGATG	1 cut(s)	207
BseLI	CCNNNNNNNGG	5 cut(s)	323, 391, 773, 1033, 1224
BseMII	CTCAG	1 cut(s)	233
BseNI	ACTGG	4 cut(s)	226, 850, 993, 1308
BseSI	GKGCMC	1 cut(s)	1027
BseXI	GCAGC	6 cut(s)	268, 985, 1082, 1208, 1211, 1335
BseYI	CCCAGC	1 cut(s)	94
BshFI	GGCC	5 cut(s)	92, 296, 992, 1025, 1217
BshNI	GGYRCC	1 cut(s)	965
BsiHKCI	CYCGRG	1 cut(s)	771
BsiSI	CCGG	4 cut(s)	421, 483, 1214, 1224
BslFI	GGGAC	1 cut(s)	1301
BslI	CCNNNNNNNGG	5 cut(s)	323, 391, 773, 1033, 1224
BsmAI	GTCTC	1 cut(s)	1049
BsmFI	GGGAC	1 cut(s)	1301
BsmI	GAATGC	3 cut(s)	118, 345, 467
BsnI	GGCC	5 cut(s)	92, 296, 992, 1025, 1217
Bso31I	GGTCTC	1 cut(s)	1049
BsoBI	CYCGRG	1 cut(s)	771
Bsp120I	GGGCCC	1 cut(s)	1023
Bsp1286I	GDGCHC	1 cut(s)	1027
Bsp13I	TCCGGA	1 cut(s)	420
Bsp143I	GATC	2 cut(s)	450, 1378
BspACI	CCGC	4 cut(s)	431, 615, 1219, 1435
BspANI	GGCC	5 cut(s)	92, 296, 992, 1025, 1217
BspCNI	CTCAG	1 cut(s)	234
BspEI	TCCGGA	1 cut(s)	420
BspHI	TCATGA	1 cut(s)	108
BspLI	GGNNCC	5 cut(s)	49, 93, 435, 967, 1025
BspMAI	CTGCAG	3 cut(s)	286, 645, 815
BspPI	GGATC	1 cut(s)	1373
BspT107I	GGYRCC	1 cut(s)	965
BspTNI	GGTCTC	1 cut(s)	1049
BsrBI	CCGCTC	1 cut(s)	1437
BsrFI	RCCGGY	2 cut(s)	482, 1213
BsrI	ACTGG	4 cut(s)	226, 850, 993, 1308
BssAI	RCCGGY	2 cut(s)	482, 1213
BssECI	CCNNGG	4 cut(s)	211, 772, 993, 1318
BssMI	GATC	2 cut(s)	450, 1378
BssNI	GRCGYC	1 cut(s)	1452
BssSI	CACGAG	1 cut(s)	1286
BssT1I	CCWWGG	1 cut(s)	993
Bst2BI	CACGAG	1 cut(s)	1286
Bst2UI	CCWGG	2 cut(s)	212, 1320
Bst4CI	ACNGT	3 cut(s)	277, 1146, 1315
Bst6I	CTCTTC	1 cut(s)	901
BstACI	GRCGYC	1 cut(s)	1452
BstC8I	GCNNGC	1 cut(s)	1219
BstDEI	CTNAG	1 cut(s)	242
BstF5I	GGATG	1 cut(s)	207
BstKTI	GATC	2 cut(s)	453, 1381
BstMAI	GTCTC	1 cut(s)	1049
BstMBI	GATC	2 cut(s)	450, 1378
BstMWI	GCNNNNNNNGC	4 cut(s)	488, 974, 998, 1253
BstNI	CCWGG	2 cut(s)	212, 1320
BstNSI	RCATGY	1 cut(s)	563
BstSCI	CCNGG	3 cut(s)	210, 1223, 1318
BstSFI	CTRYAG	3 cut(s)	282, 641, 811
BstSLI	GKGCMC	1 cut(s)	1027
BstV1I	GCAGC	6 cut(s)	268, 985, 1082, 1208, 1211, 1335
BstV2I	GAAGAC	3 cut(s)	562, 934, 1371
BstXI	CCANNNNNNTGG	1 cut(s)	685
BsuI	GTATCC	1 cut(s)	662
BsuRI	GGCC	5 cut(s)	92, 296, 992, 1025, 1217
BtsCI	GGATG	1 cut(s)	207
BtsI	GCAGTG	1 cut(s)	650
BtsIMutI	CAGTG	3 cut(s)	650, 986, 1311
Cac8I	GCNNGC	1 cut(s)	1219
CaiI	CAGNNNCTG	1 cut(s)	845
CciI	TCATGA	1 cut(s)	108
Cfr10I	RCCGGY	2 cut(s)	482, 1213
Cfr13I	GGNCC	6 cut(s)	91, 585, 1023, 1024, 1211, 1216
CseI	GACGC	1 cut(s)	1441
Csp6I	GTAC	5 cut(s)	382, 510, 843, 1007, 1301
CspCI	CAANNNNNGTGG	2 cut(s)	975, 1010
CviAII	CATG	9 cut(s)	109, 560, 571, 603, 618, 685, 1020, 1087, 1273
CviQI	GTAC	5 cut(s)	382, 510, 843, 1007, 1301
DdeI	CTNAG	1 cut(s)	242
DpnI	GATC	2 cut(s)	452, 1380
DpnII	GATC	2 cut(s)	450, 1378
Eam1104I	CTCTTC	1 cut(s)	901
EarI	CTCTTC	1 cut(s)	901
Eco130I	CCWWGG	1 cut(s)	993
Eco24I	GRGCYC	1 cut(s)	1027
Eco31I	GGTCTC	1 cut(s)	1049
Eco47I	GGWCC	2 cut(s)	585, 1211
Eco88I	CYCGRG	1 cut(s)	771
EcoO109I	RGGNCCY	1 cut(s)	1024
EcoRII	CCWGG	2 cut(s)	210, 1318
EcoT14I	CCWWGG	1 cut(s)	993
EcoT22I	ATGCAT	3 cut(s)	469, 565, 604
EcoT38I	GRGCYC	1 cut(s)	1027
ErhI	CCWWGG	1 cut(s)	993
FaeI	CATG	9 cut(s)	112, 563, 574, 606, 621, 688, 1023, 1090, 1276
FaqI	GGGAC	1 cut(s)	1301
FatI	CATG	9 cut(s)	108, 559, 570, 602, 617, 684, 1019, 1086, 1272
FauI	CCCGC	1 cut(s)	1226
FauNDI	CATATG	3 cut(s)	455, 493, 1192
FbaI	TGATCA	1 cut(s)	450
Fnu4HI	GCNGC	9 cut(s)	5, 282, 615, 999, 1071, 1197, 1200, 1324, 1435
FokI	GGATG	1 cut(s)	194
FriOI	GRGCYC	1 cut(s)	1027
Fsp4HI	GCNGC	9 cut(s)	5, 282, 615, 999, 1071, 1197, 1200, 1324, 1435
FspBI	CTAG	2 cut(s)	525, 1176
GluI	GCNGC	9 cut(s)	5, 282, 615, 999, 1071, 1197, 1200, 1324, 1435
GsaI	CCCAGC	1 cut(s)	98
HaeIII	GGCC	5 cut(s)	92, 296, 992, 1025, 1217
HapII	CCGG	4 cut(s)	421, 483, 1214, 1224
HgaI	GACGC	1 cut(s)	1441
Hin1I	GRCGYC	1 cut(s)	1452
Hin1II	CATG	9 cut(s)	112, 563, 574, 606, 621, 688, 1023, 1090, 1276
HincII	GTYRAC	1 cut(s)	852
HindII	GTYRAC	1 cut(s)	852
HinfI	GANTC	4 cut(s)	130, 628, 716, 807
HpaII	CCGG	4 cut(s)	421, 483, 1214, 1224
HphI	GGTGA	2 cut(s)	367, 1291
Hpy166II	GTNNAC	4 cut(s)	375, 397, 852, 1232
Hpy188I	TCNGA	4 cut(s)	129, 517, 759, 1457
Hpy188III	TCNNGA	7 cut(s)	109, 119, 421, 625, 670, 1331, 1422
Hpy8I	GTNNAC	4 cut(s)	375, 397, 852, 1232
HpyAV	CCTTC	1 cut(s)	395
HpyCH4III	ACNGT	3 cut(s)	277, 1146, 1315
HpyCH4IV	ACGT	1 cut(s)	1052
HpyF10VI	GCNNNNNNNGC	4 cut(s)	488, 974, 998, 1253
HpyF3I	CTNAG	1 cut(s)	242
HpySE526I	ACGT	1 cut(s)	1052
Hsp92I	GRCGYC	1 cut(s)	1452
Hsp92II	CATG	9 cut(s)	112, 563, 574, 606, 621, 688, 1023, 1090, 1276
Kpn2I	TCCGGA	1 cut(s)	420
Ksp22I	TGATCA	1 cut(s)	450
Kzo9I	GATC	2 cut(s)	450, 1378
LmnI	GCTCC	3 cut(s)	176, 488, 923
Lsp1109I	GCAGC	6 cut(s)	268, 985, 1082, 1208, 1211, 1335
LweI	GCATC	6 cut(s)	216, 418, 535, 716, 749, 1211
MaeI	CTAG	2 cut(s)	525, 1176
MaeII	ACGT	1 cut(s)	1052
MaeIII	GTNAC	1 cut(s)	1279
MalI	GATC	2 cut(s)	452, 1380
MbiI	CCGCTC	1 cut(s)	1437
MboI	GATC	2 cut(s)	450, 1378
MboII	GAAGA	7 cut(s)	567, 652, 918, 934, 1376, 1377, 1406
MhlI	GDGCHC	1 cut(s)	1027
MluCI	AATT	6 cut(s)	146, 183, 750, 777, 792, 1441
MmeI	TCCRAC	2 cut(s)	703, 791
Mph1103I	ATGCAT	3 cut(s)	469, 565, 604
MroI	TCCGGA	1 cut(s)	420
MroXI	GAANNNNTTC	2 cut(s)	870, 1417
MseI	TTAA	4 cut(s)	227, 539, 753, 935
MslI	CAYNNNNRTG	2 cut(s)	683, 1277
MspI	CCGG	4 cut(s)	421, 483, 1214, 1224
MspR9I	CCNGG	3 cut(s)	212, 1225, 1320
Mva1269I	GAATGC	3 cut(s)	118, 345, 467
MvaI	CCWGG	2 cut(s)	212, 1320
MwoI	GCNNNNNNNGC	4 cut(s)	488, 974, 998, 1253
NciI	CCSGG	1 cut(s)	1225
NdeI	CATATG	3 cut(s)	455, 493, 1192
NdeII	GATC	2 cut(s)	450, 1378
NlaIII	CATG	9 cut(s)	112, 563, 574, 606, 621, 688, 1023, 1090, 1276
NlaIV	GGNNCC	5 cut(s)	49, 93, 435, 967, 1025
NmuCI	GTSAC	1 cut(s)	1279
NsiI	ATGCAT	3 cut(s)	469, 565, 604
NspI	RCATGY	1 cut(s)	563
PagI	TCATGA	1 cut(s)	108
PctI	GAATGC	3 cut(s)	118, 345, 467
PdmI	GAANNNNTTC	2 cut(s)	870, 1417
PfeI	GAWTC	4 cut(s)	130, 628, 716, 807
PkrI	GCNGC	9 cut(s)	6, 283, 616, 1000, 1072, 1198, 1201, 1325, 1436
PshBI	ATTAAT	1 cut(s)	753
Psp1406I	AACGTT	1 cut(s)	1052
Psp6I	CCWGG	2 cut(s)	210, 1318
PspFI	CCCAGC	1 cut(s)	94
PspGI	CCWGG	2 cut(s)	210, 1318
PspN4I	GGNNCC	5 cut(s)	49, 93, 435, 967, 1025
PspOMI	GGGCCC	1 cut(s)	1023
PspPI	GGNCC	6 cut(s)	91, 585, 1023, 1024, 1211, 1216
PstI	CTGCAG	3 cut(s)	286, 645, 815
PstNI	CAGNNNCTG	1 cut(s)	845
RsaI	GTAC	5 cut(s)	383, 511, 844, 1008, 1302
RsaNI	GTAC	5 cut(s)	382, 510, 843, 1007, 1301
RseI	CAYNNNNRTG	2 cut(s)	683, 1277
SaqAI	TTAA	4 cut(s)	227, 539, 753, 935
SatI	GCNGC	9 cut(s)	5, 282, 615, 999, 1071, 1197, 1200, 1324, 1435
Sau3AI	GATC	2 cut(s)	450, 1378
Sau96I	GGNCC	6 cut(s)	91, 585, 1023, 1024, 1211, 1216
ScrFI	CCNGG	3 cut(s)	212, 1225, 1320
SduI	GDGCHC	1 cut(s)	1027
SfaNI	GCATC	6 cut(s)	216, 418, 535, 716, 749, 1211
SfcI	CTRYAG	3 cut(s)	282, 641, 811
SinI	GGWCC	2 cut(s)	585, 1211
SmiMI	CAYNNNNRTG	2 cut(s)	683, 1277
SmlI	CTYRAG	3 cut(s)	117, 318, 439
SmoI	CTYRAG	3 cut(s)	117, 318, 439
Sse9I	AATT	6 cut(s)	146, 183, 750, 777, 792, 1441
SsiI	CCGC	4 cut(s)	431, 615, 1219, 1435
SspI	AATATT	1 cut(s)	30
SspMI	CTAG	2 cut(s)	525, 1176
StyD4I	CCNGG	3 cut(s)	210, 1223, 1318
StyI	CCWWGG	1 cut(s)	993
TaaI	ACNGT	3 cut(s)	277, 1146, 1315
TaiI	ACGT	1 cut(s)	1055
TaqI	TCGA	4 cut(s)	194, 388, 904, 1066
TaqII	GACCGA	1 cut(s)	602
TasI	AATT	6 cut(s)	146, 183, 750, 777, 792, 1441
TauI	GCSGC	2 cut(s)	617, 1437
TfiI	GAWTC	4 cut(s)	130, 628, 716, 807
Tru1I	TTAA	4 cut(s)	227, 539, 753, 935
Tru9I	TTAA	4 cut(s)	227, 539, 753, 935
TscAI	CASTG	3 cut(s)	650, 993, 1318
TseFI	GTSAC	1 cut(s)	1279
TseI	GCWGC	7 cut(s)	4, 281, 998, 1070, 1196, 1199, 1323
Tsp45I	GTSAC	1 cut(s)	1279
TspDTI	ATGAA	7 cut(s)	66, 125, 863, 940, 1095, 1156, 1174
TspGWI	ACGGA	1 cut(s)	994
TspRI	CASTG	3 cut(s)	650, 993, 1318
VpaK11BI	GGWCC	2 cut(s)	585, 1211
VspI	ATTAAT	1 cut(s)	753
XapI	RAATTY	1 cut(s)	146
XceI	RCATGY	1 cut(s)	563
XmnI	GAANNNNTTC	2 cut(s)	870, 1417
XspI	CTAG	2 cut(s)	525, 1176
Zsp2I	ATGCAT	3 cut(s)	469, 565, 604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.